Restriction map for
pFN21AB5343
(Circular) (Six-base) MAPSORT of: pFN21AB5343.seq Check: 7429 from: 1 to: 6469
pFN21AB5343 6469 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3162 3162
Size: 6469
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3108 4279 278
Size: 53 83 186 936 1572 1171 2468
Fragments arranged by size:
2468 1572 1171 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3138 3812 1937
Size: 1201 674 4594
Fragments arranged by size:
4594 1201 674
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 2192 4596 4969 1563
Size: 629 2404 373 3063
Fragments arranged by size:
3063 2404 629 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 3014 3344 4532 5916 6184 1632
Size: 512 870 330 1188 1384 268 1917
Fragments arranged by size:
1917 1384 1188 870 512 330 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6272
AflIII A'CryG_T
Cuts at: 1203 2799 5368 6345 1203
Size: 1596 2569 977 1327
Fragments arranged by size:
2569 1596 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 5198 5198
Size: 6469
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6469
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3291 3323 3291
Size: 32 6437
AlwNI CAG_nnn'CTG
Cuts at: 3461 3574 4212 5784 3461
Size: 113 638 1572 4146
Fragments arranged by size:
4146 1572 638 113
ApaLI G'TGCA_C
Cuts at: 4526 5682 4526
Size: 1156 5313
ApoI r'AATT_y
Cuts at: 1493 3778 4004 4156 6265 1493
Size: 2285 226 152 2109 1697
Fragments arranged by size:
2285 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5023 160
Size: 872 3991 1606
Fragments arranged by size:
3991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 3794 1870
Size: 73 810 1041 4545
Fragments arranged by size:
4545 1041 810 73
BamHI G'GATC_C
Cuts at: 3799 4166 3799
Size: 367 6102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2792 2866 3016 3545 3790
Size: 325 217 627 1005 74 150 529 245
Cuts at: 3790 5245 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1005 627 529 325 245 217
150 74
BanII G_rGCy'C
Cuts at: 1391 3788 1391
Size: 2397 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2539 2943 928
Size: 971 640 404 4454
Fragments arranged by size:
4454 971 640 404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4660 4694 1903
Size: 34 2723 34 3678
Fragments arranged by size:
3678 2723 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2385 4361 5571 2385
Size: 1976 1210 3283
Fragments arranged by size:
3283 1976 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2852 3491 3755
Size: 107 122 71 631 605 1180 639 264
Cuts at: 3755 5080 136
Size: 1325 1525
Fragments arranged by size:
1525 1325 1180 639 631 605 264 122
107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 2090 3886 2090
Size: 1796 4673
Bme1580I G_kGCm'C
Cuts at: 1790 2536 2795 3021 4530 5686 1790
Size: 746 259 226 1509 1156 2573
Fragments arranged by size:
2573 1509 1156 746 259 226
BmgBI CAC'GTC
Cuts at: 1318 2647 1318
Size: 1329 5140
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2597 3184 4200
Size: 416 53 83 399 24 1180 587 1016
Cuts at: 4200 5158 442
Size: 958 1753
Fragments arranged by size:
1753 1180 1016 958 587 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3262 3262
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2975 3578 5129 1239
Size: 830 906 603 1551 2579
Fragments arranged by size:
2579 1551 906 830 603
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 4594 5459 5757 2036
Size: 2558 865 298 2748
Fragments arranged by size:
2748 2558 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5132 882
Size: 617 3633 2219
Fragments arranged by size:
3633 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2467 3181 4260 493
Size: 711 1263 714 1079 2702
Fragments arranged by size:
2702 1263 1079 714 711
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3105 4276 4658 5293
Size: 53 83 186 936 1572 1171 382 635
Cuts at: 5293 275
Size: 1451
Fragments arranged by size:
1572 1451 1171 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2271 2280 4902 5300 5574 5721 6132 2271
Size: 9 2622 398 274 147 411 2608
Fragments arranged by size:
2622 2608 411 398 274 147 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3040 3070 3291 3321 1178
Size: 30 165 30 1637 30 221 30 4326
Fragments arranged by size:
4326 1637 221 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2894 2897 3395 3398 3452 3455 3458
Size: 1027 681 3 498 3 54 3 3
Cuts at: 3458 3599 3602 1186
Size: 141 3 4053
Fragments arranged by size:
4053 1027 681 498 141 54 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2695 5672 6168 2695
Size: 2977 496 2996
Fragments arranged by size:
2996 2977 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2489 3203 3275 1163
Size: 573 753 714 72 4357
Fragments arranged by size:
4357 753 714 573 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3919 4680 5708 1540
Size: 275 186 1141 777 761 1028 2301
Fragments arranged by size:
2301 1141 1028 777 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4530 4615 5686 4530
Size: 85 1071 5313
Fragments arranged by size:
5313 1071 85
BsmI GAATG_Cn'
Cuts at: 2118 3980 4073 2118
Size: 1862 93 4514
Fragments arranged by size:
4514 1862 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2536 2795 3021 3788 4530 4615 5686
Size: 399 746 259 226 767 742 85 1071
Cuts at: 5686 1391
Size: 2174
Fragments arranged by size:
2174 1071 767 746 742 399 259 226
85
BspEI T'CCGG_A
Cuts at: 2271 2280 6132 2271
Size: 9 3852 2608
Fragments arranged by size:
3852 2608 9
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3162 3824 844
Size: 2318 662 3489
Fragments arranged by size:
3489 2318 662
BsrBI CCG'CTC
Cuts at: 1394 1823 4356 1394
Size: 429 2533 3507
Fragments arranged by size:
3507 2533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4964 5138 65
Size: 1183 3716 174 1396
Fragments arranged by size:
3716 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2490 2783 3141 5113 5300 1700
Size: 110 680 293 358 1972 187 2869
Fragments arranged by size:
2869 1972 680 358 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 4529 5541 4529
Size: 1012 5457
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6469
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6469
BstXI CCAn_nnnn'nTGG
Cuts at: 2695 3538 3607 3697 2695
Size: 843 69 90 5467
Fragments arranged by size:
5467 843 90 69
BstYI r'GATC_y
Cuts at: 1972 3799 4166 4551 4568 6020 6106 6118 6464
Size: 1827 367 385 17 1452 86 12 346
Cuts at: 6464 1972
Size: 1977
Fragments arranged by size:
1977 1827 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2447 3231 513
Size: 552 939 443 784 3751
Fragments arranged by size:
3751 939 784 552 443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5730
Fragments arranged by size:
5730 683 56
BtsI GCAGTG_nn'
Cuts at: 3985 4779 4799 3985
Size: 794 20 5655
Fragments arranged by size:
5655 794 20
ClaI AT'CG_AT
Cuts at: 1554 2388 1554
Size: 834 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 2325 3773 2325
Size: 1448 5021
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2657 5476 809
Size: 507 1341 2819 1802
Fragments arranged by size:
2819 1802 1341 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2664 3609
Size: 54 1425 50 275 18 213 621 945
Cuts at: 3609 3916 4805 6434 8
Size: 307 889 1629 43
Fragments arranged by size:
1629 1425 945 889 621 307 275 213
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 3372 3384 3417 3474 3498 4399 1353
Size: 938 1081 12 33 57 24 901 3423
Fragments arranged by size:
3423 1081 938 901 57 33 24 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2702 3716 5056 5430 5576 387
Size: 1064 138 1113 1014 1340 374 146 1280
Fragments arranged by size:
1340 1280 1113 1064 1014 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2144 2975 3014 3344 3578 4532
Size: 393 437 75 831 39 330 234 954
Cuts at: 4532 5129 5916 6184 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 954 831 787 597 437 393 330
268 234 75 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2540 2540
Size: 6469
EcoO109I rG'GnC_Cy
Cuts at: 2401 2989 3028 3352 2401
Size: 588 39 324 5518
Fragments arranged by size:
5518 588 324 39
EcoRI G'AATT_C
Cuts at: 3778 4156 3778
Size: 378 6091
FspI TGC'GCA
Cuts at: 2540 4975 2540
Size: 2435 4034
HaeII r_GCGC'y
Cuts at: 1678 3607 5616 1678
Size: 1929 2009 2531
Fragments arranged by size:
2531 2009 1929
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 5110 5142 5184
Size: 32 135 32 830 32 2676 32 42
Cuts at: 5184 5216 1373
Size: 32 2626
Fragments arranged by size:
2676 2626 830 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2167 2242 2707 3139 3813 4059
Size: 988 281 229 75 465 432 674 246
Cuts at: 4059 669
Size: 3079
Fragments arranged by size:
3079 988 674 465 432 281 246 229
75
HindIII A'AGCT_T
Cuts at: 748 2978 748
Size: 2230 4239
HpaI GTT'AAC
Cuts at: 2167 4059 2167
Size: 1892 4577
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 2799 6345 2799
Size: 3546 2923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2784 5583 5767 1305
Size: 1479 2799 184 2007
Fragments arranged by size:
2799 2007 1479 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2666 3611 10
Size: 54 1425 343 834 945 2868
Fragments arranged by size:
2868 1425 945 834 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3617 4427 4786 4945 518
Size: 648 2451 810 359 159 2042
Fragments arranged by size:
2451 2042 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2449 2482 3259 3343 3571 3885 4562
Size: 483 360 33 777 84 228 314 677
Cuts at: 4562 5710 5955 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 777 677 483 360 314 245
228 84 33
NaeI GCC'GGC
Cuts at: 2492 2492
Size: 6469
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4978
Fragments arranged by size:
4978 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NgoMIV G'CCGG_C
Cuts at: 2490 2490
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 3827 5372 1132
Size: 97 2598 1545 2229
Fragments arranged by size:
2598 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3531 1348
Size: 2183 4286
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2604 1108
Size: 1496 4973
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PmlI CAC'GTG
Cuts at: 2467 2467
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3291 3323 4538 4570 6051 6083 3291
Size: 32 1215 32 1481 32 3677
Fragments arranged by size:
3677 1481 1215 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2401 3352 2401
Size: 951 5518
PshAI GACnn'nnGTC
Cuts at: 1613 3161 1613
Size: 1548 4921
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PspXI vC'TCGA_Gb
Cuts at: 2753 2753
Size: 6469
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2366 2398 2366
Size: 32 6437
PstI C_TGCA'G
Cuts at: 830 3342 3821 830
Size: 2512 479 3478
Fragments arranged by size:
3478 2512 479
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
PvuII CAG'CTG
Cuts at: 2089 2482 3259 2089
Size: 393 777 5299
Fragments arranged by size:
5299 777 393
SacII CC_GC'GG
Cuts at: 2450 2450
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3137 3811 1936
Size: 1201 674 4594
Fragments arranged by size:
4594 1201 674
SapI GCTCTTCn'nnn_
Cuts at: 2291 3498 2291
Size: 1207 5262
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 3242 4717 6429 3242
Size: 1475 1712 3282
Fragments arranged by size:
3282 1712 1475
SfcI C'TryA_G
Cuts at: 826 1045 2178 2313 2653 3338 3817 4952 5633
Size: 219 1133 135 340 685 479 1135 681
Cuts at: 5633 5824 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1133 685 681 479 340 219
191 135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 2753 4573 5474 5736 820
Size: 197 926 72 738 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 738 262 197 72
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3827 1132
Size: 2695 3774
SspI AAT'ATT
Cuts at: 5 52 4393 5
Size: 47 4341 2081
Fragments arranged by size:
4341 2081 47
StuI AGG'CCT
Cuts at: 1517 2013 1517
Size: 496 5973
StyI C'CwwG_G
Cuts at: 513 1065 2004 3908 513
Size: 552 939 1904 3074
Fragments arranged by size:
3074 1904 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3140 3184 3302 4496 4513 4666 4851
Size: 1255 1560 44 118 1194 17 153 185
Cuts at: 4851 5296 325
Size: 445 1498
Fragments arranged by size:
1560 1498 1255 1194 445 185 153 118
44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3240 4715 6427
Size: 274 80 33 51 1443 1263 1475 1712
Cuts at: 6427 96
Size: 138
Fragments arranged by size:
1712 1475 1443 1263 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3040 3072 6429 6461 3040
Size: 32 3357 32 3048
Fragments arranged by size:
3357 3048 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6469
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 2753 1943
Size: 810 5659
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 2999 4598 2999
Size: 1599 4870
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3106 4277 276
Size: 53 83 186 936 1572 1171 2468
Fragments arranged by size:
2468 1572 1171 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PspOMI RsrII SacI SanDI SexAI SfiI SfoI
SrfI SwaI XcmI