Restriction map for
pFN21AB5341
(Circular) (Six-base) MAPSORT of: pFN21AB5341.seq Check: 447 from: 1 to: 5743
pFN21AB5341 5743 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 2723 3806 5190 5458 1632
Size: 669 422 1083 1384 268 1917
Fragments arranged by size:
1917 1384 1083 669 422 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2886 3486 5058 2886
Size: 600 1572 3571
Fragments arranged by size:
3571 1572 600
ApaI G_GGCC'C
Cuts at: 2580 2580
Size: 5743
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 3068 1870
Size: 73 172 953 4545
Fragments arranged by size:
4545 953 172 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 4519 618
Size: 325 217 627 1277 1455 1842
Fragments arranged by size:
1842 1455 1277 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2239 2302 2580 3062 1391
Size: 848 63 278 482 4072
Fragments arranged by size:
4072 848 482 278 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2632 928
Size: 971 733 4039
Fragments arranged by size:
4039 971 733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2486 3635 4845 2486
Size: 1149 1210 3384
Fragments arranged by size:
3384 1210 1149
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4354 136
Size: 107 122 71 631 605 2682 1525
Fragments arranged by size:
2682 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2436 5738 2436
Size: 3302 2441
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2580 3804 4960 1790
Size: 790 1224 1156 2573
Fragments arranged by size:
2573 1224 1156 790
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2265 2769 2852
Size: 416 53 83 399 24 848 504 83
Cuts at: 2852 2900 3474 4432 442
Size: 48 574 958 1753
Fragments arranged by size:
1753 958 848 574 504 416 399 83
83 53 48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2205 2392 2684 2695 2798 4403 1239
Size: 869 97 187 292 11 103 1605 2579
Fragments arranged by size:
2579 1605 869 292 187 103 97 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3868 4733 5031 3868
Size: 865 298 4580
Fragments arranged by size:
4580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3550 3932 4567 275
Size: 53 83 186 936 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2876 2906 1178
Size: 30 165 30 1473 30 4015
Fragments arranged by size:
4015 1473 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2057 2060 2546 2964 1186
Size: 847 24 3 486 418 3965
Fragments arranged by size:
3965 847 486 418 24 3
BseYI C'CCAG_C
Cuts at: 4946 5442 4946
Size: 496 5247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2239 2302 2742 3804 3889 4960 2239
Size: 63 440 1062 85 1071 3022
Fragments arranged by size:
3022 1071 1062 440 85 63
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2302 2580 2742 3062 3804 3889
Size: 399 449 63 278 162 320 742 85
Cuts at: 3889 4960 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 449 399 320 278 162
85 63
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2868 3098 844
Size: 2024 230 3489
Fragments arranged by size:
3489 2024 230
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 4387 4574 1700
Size: 110 254 2323 187 2869
Fragments arranged by size:
2869 2323 254 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2696 794
Size: 1902 3841
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2070 2227 2436 2770 3073 3440 3825 3842
Size: 98 157 209 334 303 367 385 17
Cuts at: 3842 5294 5380 5392 5738 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 334 303 209
157 98 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2868 2868
Size: 5743
BtgI C'CryG_G
Cuts at: 513 1065 2004 2316 2488 513
Size: 552 939 312 172 3768
Fragments arranged by size:
3768 939 552 312 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2686 3259 4053 4073 2686
Size: 573 794 20 4356
Fragments arranged by size:
4356 794 573 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2266 3190 4079
Size: 54 1425 50 275 18 436 924 889
Cuts at: 4079 5708 8
Size: 1629 43
Fragments arranged by size:
1629 1425 924 889 436 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2761 3673 1353
Size: 1408 912 3423
Fragments arranged by size:
3423 1408 912
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 4330 4704 4850 387
Size: 1064 138 474 2267 374 146 1280
Fragments arranged by size:
2267 1280 1064 474 374 146 138
EcoICRI GAG'CTC
Cuts at: 2237 2300 2237
Size: 63 5680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2205 2301 2392 2684 2695 2723
Size: 393 476 97 96 91 292 11 28
Cuts at: 2723 2798 3806 4403 5190 5458 1239
Size: 75 1008 597 787 268 1524
Fragments arranged by size:
1524 1008 787 597 476 393 292 268
97 96 91 75 28 11
EcoO109I rG'GnC_Cy
Cuts at: 2953 2968 2953
Size: 15 5728
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 5743
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 4890 1678
Size: 3212 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4384 4416 4458 4490 1373
Size: 32 135 32 2812 32 42 32 2626
Fragments arranged by size:
2812 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2431 3087 3333 669
Size: 988 281 493 656 246 3079
Fragments arranged by size:
3079 988 656 493 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2742 2902 4857 5041 1305
Size: 1437 160 1955 184 2007
Fragments arranged by size:
2007 1955 1437 184 160
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3701 4060 4219 518
Size: 648 2535 359 159 2042
Fragments arranged by size:
2535 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2965 3159 3836 4984 5229 1606
Size: 1359 194 677 1148 245 2120
Fragments arranged by size:
2120 1359 1148 677 245 194
NcoI C'CATG_G
Cuts at: 513 1065 2004 2316 2488 513
Size: 552 939 312 172 3768
Fragments arranged by size:
3768 939 552 312 172
NdeI CA'TA_TG
Cuts at: 387 2788 387
Size: 2401 3342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5743
PfoI T'CCnGG_A
Cuts at: 2230 2230
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2968 2968
Size: 5743
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2576 2576
Size: 5743
PstI C_TGCA'G
Cuts at: 830 3095 830
Size: 2265 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SacI G_AGCT'C
Cuts at: 2239 2302 2239
Size: 63 5680
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SanDI GG'GwC_CC
Cuts at: 2968 2968
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 3091 4226 4907 5098 826
Size: 219 2046 1135 681 191 1471
Fragments arranged by size:
2046 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3847 4748 5010 820
Size: 197 926 1904 901 262 1553
Fragments arranged by size:
1904 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2004 2316 2488 2560 3031 3039 3182
Size: 552 939 312 172 72 471 8 143
Cuts at: 3182 513
Size: 3074
Fragments arranged by size:
3074 939 552 471 312 172 143 72
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3989 5701 96
Size: 274 80 33 51 1443 2012 1712 138
Fragments arranged by size:
2012 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 2876 1316
Size: 1560 4183
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI