Restriction map for
pFN21AB5339
(Circular) (Six-base) MAPSORT of: pFN21AB5339.seq Check: 8711 from: 1 to: 6307
pFN21AB5339 6307 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2459 2459
Size: 6307
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3650 1937
Size: 1713 4594
Acc65I G'GTAC_C
Cuts at: 2236 3628 2236
Size: 1392 4915
AclI AA'CG_TT
Cuts at: 1563 4434 4807 1563
Size: 2871 373 3063
Fragments arranged by size:
3063 2871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 2757 3251 3570 4370 5754 6022 1632
Size: 918 207 494 319 800 1384 268 1917
Fragments arranged by size:
1917 1384 918 800 494 319 268 207
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6110
AflIII A'CryG_T
Cuts at: 1203 2371 5206 6183 1203
Size: 1168 2835 977 1327
Fragments arranged by size:
2835 1327 1168 977
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 5036 5036
Size: 6307
AleI CACnn'nnGTG
Cuts at: 1166 2765 1166
Size: 1599 4708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6307
AlwNI CAG_nnn'CTG
Cuts at: 3145 4050 5622 3145
Size: 905 1572 3830
Fragments arranged by size:
3830 1572 905
ApaLI G'TGCA_C
Cuts at: 4364 5520 4364
Size: 1156 5151
ApoI r'AATT_y
Cuts at: 1493 2413 2432 2513 3071 3290 3455 3616 3842
Size: 920 19 81 558 219 165 161 226
Cuts at: 3842 3994 6103 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 920 558 226 219 165 161
152 81 19
AseI AT'TA_AT
Cuts at: 160 1032 4861 160
Size: 872 3829 1606
Fragments arranged by size:
3829 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 3632 1870
Size: 73 1689 4545
Fragments arranged by size:
4545 1689 73
BamHI G'GATC_C
Cuts at: 3637 4004 3637
Size: 367 5940
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2236 3628 5083 618
Size: 325 217 627 224 225 1392 1455 1842
Fragments arranged by size:
1842 1455 1392 627 325 225 224 217
BanII G_rGCy'C
Cuts at: 1391 2641 3626 1391
Size: 1250 985 4072
Fragments arranged by size:
4072 1250 985
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 6307
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4498 4532 1903
Size: 34 2561 34 3678
Fragments arranged by size:
3678 2561 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4199 5409 4199
Size: 1210 5097
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6307
BfrBI ATG'CAT
Cuts at: 2779 2779
Size: 6307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4918 136
Size: 107 122 71 631 605 337 2909 1525
Fragments arranged by size:
2909 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6302 6302
Size: 6307
BlpI GC'TnA_GC
Cuts at: 2329 3724 2329
Size: 1395 4912
Bme1580I G_kGCm'C
Cuts at: 1790 4368 5524 1790
Size: 2578 1156 2573
Fragments arranged by size:
2578 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6307
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2312 4038 4996
Size: 416 53 83 399 24 895 1726 958
Cuts at: 4996 442
Size: 1753
Fragments arranged by size:
1753 1726 958 895 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2621 1056
Size: 1565 4742
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2213 2213
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4967 1239
Size: 3728 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4432 5297 5595 4432
Size: 865 298 5144
Fragments arranged by size:
5144 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4970 882
Size: 617 3471 2219
Fragments arranged by size:
3471 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4098 493
Size: 711 2894 2702
Fragments arranged by size:
2894 2702 711
BsaBI GATnn'nnATC
Cuts at: 6301 6301
Size: 6307
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2027 3161 4114
Size: 53 83 186 936 479 15 1134 953
Cuts at: 4114 4496 5131 275
Size: 382 635 1451
Fragments arranged by size:
1451 1134 953 936 635 479 382 186
83 53 15
BsaWI w'CCGG_w
Cuts at: 2361 4740 5138 5412 5559 5970 2361
Size: 2379 398 274 147 411 2698
Fragments arranged by size:
2698 2379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3195 3225 1178
Size: 30 165 30 1792 30 4260
Fragments arranged by size:
4260 1792 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 3331 1186
Size: 937 1208 4162
Fragments arranged by size:
4162 1208 937
BseYI C'CCAG_C
Cuts at: 2674 3484 5510 6006 2674
Size: 810 2026 496 2975
Fragments arranged by size:
2975 2026 810 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5734
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3757 4518 5546 1540
Size: 275 186 1756 761 1028 2301
Fragments arranged by size:
2301 1756 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2330 2361 4368 4453 5524 2330
Size: 31 2007 85 1071 3113
Fragments arranged by size:
3113 2007 1071 85 31
BsmI GAATG_Cn'
Cuts at: 2548 2776 3818 3911 2548
Size: 228 1042 93 4944
Fragments arranged by size:
4944 1042 228 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2018 2018
Size: 6307
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2361 2641 3626 4368 4453 5524
Size: 399 540 31 280 985 742 85 1071
Cuts at: 5524 1391
Size: 2174
Fragments arranged by size:
2174 1071 985 742 540 399 280 85
31
BspEI T'CCGG_A
Cuts at: 5970 5970
Size: 6307
BspHI T'CATG_A
Cuts at: 4196 5926 4196
Size: 1730 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2459 3662 844
Size: 1615 1203 3489
Fragments arranged by size:
3489 1615 1203
BsrBI CCG'CTC
Cuts at: 1394 1823 4194 1394
Size: 429 2371 3507
Fragments arranged by size:
3507 2371 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3489 4802 4976 65
Size: 1183 2241 1313 174 1396
Fragments arranged by size:
2241 1396 1313 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4951 5138 1700
Size: 110 3141 187 2869
Fragments arranged by size:
3141 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6307
BssHII G'CGCG_C
Cuts at: 1925 2080 1925
Size: 155 6152
BssSI C'ACGA_G
Cuts at: 4367 5379 4367
Size: 1012 5295
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2588 794
Size: 1794 4513
BstBI TT'CG_AA
Cuts at: 2511 5111 2511
Size: 2600 3707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6307
BstYI r'GATC_y
Cuts at: 1972 3637 4004 4389 4406 5858 5944 5956 6302
Size: 1665 367 385 17 1452 86 12 346
Cuts at: 6302 1972
Size: 1977
Fragments arranged by size:
1977 1665 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3423 3423
Size: 6307
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 513
Size: 552 939 15 4801
Fragments arranged by size:
4801 939 552 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2475 2789 487
Size: 683 56 1249 314 4005
Fragments arranged by size:
4005 1249 683 314 56
BtsI GCAGTG_nn'
Cuts at: 3130 3286 3352 3823 4617 4637 3130
Size: 156 66 471 794 20 4800
Fragments arranged by size:
4800 794 471 156 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6272
DraI TTT'AAA
Cuts at: 2518 3069 3580 3611 2518
Size: 551 511 31 5214
Fragments arranged by size:
5214 551 511 31
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5314 809
Size: 507 3998 1802
Fragments arranged by size:
3998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3127 3754 4643
Size: 54 1425 50 275 18 1297 627 889
Cuts at: 4643 6272 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1297 889 627 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3754 1537
Size: 275 1942 4090
Fragments arranged by size:
4090 1942 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2733 3366 4237 1353
Size: 1380 633 871 3423
Fragments arranged by size:
3423 1380 871 633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4894 5268 5414 387
Size: 1064 138 3305 374 146 1280
Fragments arranged by size:
3305 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2550 2757 3251 3570 4370 4967 5754
Size: 393 918 207 494 319 800 597 787
Cuts at: 5754 6022 1239
Size: 268 1524
Fragments arranged by size:
1524 918 800 787 597 494 393 319
268 207
EcoRI G'AATT_C
Cuts at: 2432 3616 3994 2432
Size: 1184 378 4745
Fragments arranged by size:
4745 1184 378
FspI TGC'GCA
Cuts at: 4813 4813
Size: 6307
HaeII r_GCGC'y
Cuts at: 1678 2015 5454 1678
Size: 337 3439 2531
Fragments arranged by size:
3439 2531 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2588 2620 2714
Size: 32 135 32 632 32 352 32 94
Cuts at: 2714 2746 3195 3227 4948 4980 5022 5054 1373
Size: 32 449 32 1721 32 42 32 2626
Fragments arranged by size:
2626 1721 632 449 352 135 94 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2482 3651 3897 669
Size: 988 281 544 1169 246 3079
Fragments arranged by size:
3079 1169 988 544 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6307
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 6307
KpnI G_GTAC'C
Cuts at: 2240 3632 2240
Size: 1392 4915
MfeI C'AATT_G
Cuts at: 3906 3906
Size: 6307
MluI A'CGCG_T
Cuts at: 6183 6183
Size: 6307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2962 3519 5421 5605 1305
Size: 1657 557 1902 184 2007
Fragments arranged by size:
2007 1902 1657 557 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3129 10
Size: 54 1425 343 1297 3188
Fragments arranged by size:
3188 1425 1297 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2283 2765 2814 2898 4265 4624 4783
Size: 648 1117 482 49 84 1367 359 159
Cuts at: 4783 518
Size: 2042
Fragments arranged by size:
2042 1367 1117 648 482 359 159 84
49
MspA1I CmG'CkG
Cuts at: 1606 3327 3723 4400 5548 5793 1606
Size: 1721 396 677 1148 245 2120
Fragments arranged by size:
2120 1721 1148 677 396 245
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 6307
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4816
Fragments arranged by size:
4816 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6307
NheI G'CTAG_C
Cuts at: 1052 2617 1052
Size: 1565 4742
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NsiI A_TGCA'T
Cuts at: 2781 2781
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 2375 3665 5210 1132
Size: 97 1146 1290 1545 2229
Fragments arranged by size:
2229 1545 1290 1146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6307
PciI A'CATG_T
Cuts at: 2371 5206 2371
Size: 2835 3472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2840 1108
Size: 1732 4575
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6307
PsiI TTA'TAA
Cuts at: 2226 3877 2226
Size: 1651 4656
PstI C_TGCA'G
Cuts at: 830 2470 3169 3659 830
Size: 1640 699 490 3478
Fragments arranged by size:
3478 1640 699 490
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
PvuII CAG'CTG
Cuts at: 3327 3327
Size: 6307
SalI G'TCGA_C
Cuts at: 1936 3649 1936
Size: 1713 4594
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 4555 6267 4555
Size: 1712 4595
SfcI C'TryA_G
Cuts at: 826 1045 2343 2466 3165 3655 4790 5471 5662
Size: 219 1298 123 699 490 1135 681 191
Cuts at: 5662 826
Size: 1471
Fragments arranged by size:
1471 1298 1135 699 681 490 219 191
123
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 6307
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6307
SmaI CCC'GGG
Cuts at: 1872 3634 1872
Size: 1762 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4411 5312 5574 820
Size: 197 926 2468 901 262 1553
Fragments arranged by size:
2468 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4098 493
Size: 3605 2702
SpeI A'CTAG_T
Cuts at: 152 3269 152
Size: 3117 3190
SphI G_CATG'C
Cuts at: 1132 3665 1132
Size: 2533 3774
SspI AAT'ATT
Cuts at: 5 52 4231 5
Size: 47 4179 2081
Fragments arranged by size:
4179 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6307
StyI C'CwwG_G
Cuts at: 513 1065 2004 2607 3746 513
Size: 552 939 603 1139 3074
Fragments arranged by size:
3074 1139 939 603 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 4334 4351 4504 4689 5134 325
Size: 1255 1086 1668 17 153 185 445 1498
Fragments arranged by size:
1668 1498 1255 1086 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4553 6265 96
Size: 274 80 33 51 1443 2576 1712 138
Fragments arranged by size:
2576 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6267 6299 6267
Size: 32 6275
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6307
XbaI T'CTAG_A
Cuts at: 3643 3643
Size: 6307
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6307
XmaI C'CCGG_G
Cuts at: 1870 3632 1870
Size: 1762 4545
XmnI GAAnn'nnTTC
Cuts at: 4436 4436
Size: 6307
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI