Restriction map for
pFN21AB5334
(Circular) (Six-base) MAPSORT of: pFN21AB5334.seq Check: 1108 from: 1 to: 6271
pFN21AB5334 6271 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 278
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3614 1937
Size: 1677 4594
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2891 4334 5718 5986 1632
Size: 416 843 1443 1384 268 1917
Fragments arranged by size:
1917 1443 1384 843 416 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 5170 6147 1203
Size: 3967 977 1327
Fragments arranged by size:
3967 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 2759 3115 1166
Size: 1593 356 4322
Fragments arranged by size:
4322 1593 356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6271
AlwNI CAG_nnn'CTG
Cuts at: 2104 4014 5586 2104
Size: 1910 1572 2789
Fragments arranged by size:
2789 1910 1572
ApaLI G'TGCA_C
Cuts at: 2110 4328 5484 2110
Size: 2218 1156 2897
Fragments arranged by size:
2897 2218 1156
ApoI r'AATT_y
Cuts at: 1493 2437 3580 3806 3958 6067 1493
Size: 944 1143 226 152 2109 1697
Fragments arranged by size:
2109 1697 1143 944 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4825 160
Size: 872 3793 1606
Fragments arranged by size:
3793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 3596 1870
Size: 73 111 1542 4545
Fragments arranged by size:
4545 1542 111 73
AvrII C'CTAG_G
Cuts at: 3296 3296
Size: 6271
BamHI G'GATC_C
Cuts at: 2164 3601 3968 2164
Size: 1437 367 4467
Fragments arranged by size:
4467 1437 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2651 3592 5047 618
Size: 325 217 627 263 601 941 1455 1842
Fragments arranged by size:
1842 1455 941 627 601 325 263 217
BanII G_rGCy'C
Cuts at: 1391 3590 1391
Size: 2199 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4462 4496 1903
Size: 34 2525 34 3678
Fragments arranged by size:
3678 2525 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2955 4163 5373 2955
Size: 1208 1210 3853
Fragments arranged by size:
3853 1210 1208
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6271
BfrBI ATG'CAT
Cuts at: 2722 2722
Size: 6271
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4882 136
Size: 107 122 71 631 605 3210 1525
Fragments arranged by size:
3210 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2902 6266 2902
Size: 3364 2907
BlpI GC'TnA_GC
Cuts at: 3688 3688
Size: 6271
Bme1580I G_kGCm'C
Cuts at: 1790 2055 2114 2486 2654 4332 5488 1790
Size: 265 59 372 168 1678 1156 2573
Fragments arranged by size:
2573 1678 1156 372 265 168 59
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6271
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2386 4002 4960
Size: 416 53 83 399 24 969 1616 958
Cuts at: 4960 442
Size: 1753
Fragments arranged by size:
1753 1616 969 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2758 2332
Size: 426 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2196 2282 3309 4931 1239
Size: 827 130 86 1027 1622 2579
Fragments arranged by size:
2579 1622 1027 827 130 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4396 5261 5559 4396
Size: 865 298 5108
Fragments arranged by size:
5108 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2180 2549 3372 3433 4934 882
Size: 617 681 369 823 61 1501 2219
Fragments arranged by size:
2219 1501 823 681 617 369 61
BsaAI yAC'GTr
Cuts at: 493 1204 4062 493
Size: 711 2858 2702
Fragments arranged by size:
2858 2702 711
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3416 4078 4460 5095
Size: 53 83 186 936 1883 662 382 635
Cuts at: 5095 275
Size: 1451
Fragments arranged by size:
1883 1451 936 662 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4704 5102 5376 5523 5934 4704
Size: 398 274 147 411 5041
Fragments arranged by size:
5041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6046
Fragments arranged by size:
6046 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6271
BseYI C'CCAG_C
Cuts at: 2071 3419 3530 5474 5970 2071
Size: 1348 111 1944 496 2372
Fragments arranged by size:
2372 1944 1348 496 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5698
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3721 4482 5510 1540
Size: 275 186 1720 761 1028 2301
Fragments arranged by size:
2301 1720 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2114 4332 4417 5488 2114
Size: 2218 85 1071 2897
Fragments arranged by size:
2897 2218 1071 85
BsmI GAATG_Cn'
Cuts at: 2287 2545 3782 3875 2287
Size: 258 1237 93 4683
Fragments arranged by size:
4683 1237 258 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2051 3407 2051
Size: 1356 4915
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2114 2486 2654 3590 4332 4417
Size: 399 265 59 372 168 936 742 85
Cuts at: 4417 5488 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 936 742 399 372 265 168
85 59
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 4160 5890 4160
Size: 1730 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3626 844
Size: 2782 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2697 4158 1394
Size: 429 874 1461 3507
Fragments arranged by size:
3507 1461 874 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4766 4940 65
Size: 1183 3518 174 1396
Fragments arranged by size:
3518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3555 4915 5102 1700
Size: 110 1745 1360 187 2869
Fragments arranged by size:
2869 1745 1360 187 110
BsrGI T'GTAC_A
Cuts at: 96 3189 96
Size: 3093 3178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2104 2719 794
Size: 1310 615 4346
Fragments arranged by size:
4346 1310 615
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6271
BstXI CCAn_nnnn'nTGG
Cuts at: 3117 3117
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2164 2902 3030 3601 3968 4353 4370 5822
Size: 192 738 128 571 367 385 17 1452
Cuts at: 5822 5908 5920 6266 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 738 571 385 367 346 192
128 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2093 2646 2093
Size: 553 5718
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 3787 4581 4601 3787
Size: 794 20 5457
Fragments arranged by size:
5457 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6236
DraI TTT'AAA
Cuts at: 2929 3575 2929
Size: 646 5625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3155 5278 809
Size: 507 1839 2123 1802
Fragments arranged by size:
2123 1839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2039 2322 3542
Size: 54 1425 50 275 18 209 283 1220
Cuts at: 3542 3553 3718 4607 6236 8
Size: 11 165 889 1629 43
Fragments arranged by size:
1629 1425 1220 889 283 275 209 165
54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 3718 1537
Size: 275 1906 4090
Fragments arranged by size:
4090 1906 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 3191 4201 1353
Size: 825 1013 1010 3423
Fragments arranged by size:
3423 1013 1010 825
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4858 5232 5378 387
Size: 1064 138 3269 374 146 1280
Fragments arranged by size:
3269 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2066 2196 2282 2891 3309 4334
Size: 393 416 18 130 86 609 418 1025
Cuts at: 4334 4931 5718 5986 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1025 787 609 597 418 416 393
268 130 86 18
EcoO109I rG'GnC_Cy
Cuts at: 2426 3473 2426
Size: 1047 5224
EcoRI G'AATT_C
Cuts at: 3580 3958 3580
Size: 378 5893
FspI TGC'GCA
Cuts at: 2469 4777 2469
Size: 2308 3963
FspAI rTGC'GCAy
Cuts at: 2469 2469
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 2258 5418 1678
Size: 580 3160 2531
Fragments arranged by size:
3160 2531 580
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2548 2580 3369 3401 4912
Size: 32 135 32 976 32 789 32 1511
Cuts at: 4912 4944 4986 5018 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1511 976 789 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3615 3861 669
Size: 988 281 1320 357 246 3079
Fragments arranged by size:
3079 1320 988 357 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6271
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2607 3061 3355 5385 5569 1305
Size: 1302 454 294 2030 184 2007
Fragments arranged by size:
2030 2007 1302 454 294 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2324 10
Size: 54 1425 343 492 3957
Fragments arranged by size:
3957 1425 492 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2759 3115 4229 4588 4747 518
Size: 648 1593 356 1114 359 159 2042
Fragments arranged by size:
2042 1593 1114 648 359 356 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2573 2839 2938 3687 4364 5512 5757
Size: 438 529 266 99 749 677 1148 245
Cuts at: 5757 1606
Size: 2120
Fragments arranged by size:
2120 1148 749 677 529 438 266 245
99
NaeI GCC'GGC
Cuts at: 3557 3557
Size: 6271
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5719
NdeI CA'TA_TG
Cuts at: 387 2200 2472 387
Size: 1813 272 4186
Fragments arranged by size:
4186 1813 272
NgoMIV G'CCGG_C
Cuts at: 3555 3555
Size: 6271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6271
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NsiI A_TGCA'T
Cuts at: 2724 2724
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 2722 3629 5174 1132
Size: 97 1493 907 1545 2229
Fragments arranged by size:
2229 1545 1493 907 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 5170 5170
Size: 6271
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3551 1108
Size: 2443 3828
PfoI T'CCnGG_A
Cuts at: 3325 3469 3325
Size: 144 6127
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2496 2528 4340 4372 5853 5885 2496
Size: 32 1812 32 1481 32 2882
Fragments arranged by size:
2882 1812 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3473 3473
Size: 6271
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3009 3041 3009
Size: 32 6239
PstI C_TGCA'G
Cuts at: 830 2715 3623 830
Size: 1885 908 3478
Fragments arranged by size:
3478 1885 908
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 2573 2839 2938 2573
Size: 266 99 5906
Fragments arranged by size:
5906 266 99
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 4519 6231 4519
Size: 1712 4559
SfcI C'TryA_G
Cuts at: 826 1045 2515 2711 3619 4754 5435 5626 826
Size: 219 1470 196 908 1135 681 191 1471
Fragments arranged by size:
1471 1470 1135 908 681 219 196 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4375 5276 5538 820
Size: 197 926 2432 901 262 1553
Fragments arranged by size:
2432 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 3629 1132
Size: 2497 3774
SspI AAT'ATT
Cuts at: 5 52 4195 5
Size: 47 4143 2081
Fragments arranged by size:
4143 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6271
StyI C'CwwG_G
Cuts at: 513 1065 2190 2377 3296 3710 513
Size: 552 1125 187 919 414 3074
Fragments arranged by size:
3074 1125 919 552 414 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4298 4315 4468 4653 5098 325
Size: 1255 2718 17 153 185 445 1498
Fragments arranged by size:
2718 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2715 3189 4517
Size: 274 80 33 51 1443 738 474 1328
Cuts at: 4517 6229 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1328 738 474 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6231 6263 6231
Size: 32 6239
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6271
XbaI T'CTAG_A
Cuts at: 3607 3607
Size: 6271
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 4400 4400
Size: 6271
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 276
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI KasI NarI NruI PacI PmlI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI