Restriction map for
pFN21AB5331
(Circular) (Six-base) MAPSORT of: pFN21AB5331.seq Check: 9709 from: 1 to: 5950
pFN21AB5331 5950 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3075 3760 278
Size: 53 83 186 936 1539 685 2468
Fragments arranged by size:
2468 1539 936 685 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3090 3293 1937
Size: 1153 203 4594
Fragments arranged by size:
4594 1153 203
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2819 4013 5397 5665 1632
Size: 1187 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1194 1187 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 2571 2712 4849 5826 1203
Size: 1368 141 2137 977 1327
Fragments arranged by size:
2137 1368 1327 977 141
AgeI A'CCGG_T
Cuts at: 2216 4781 2216
Size: 2565 3385
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2103 2963 1184
Size: 919 860 4171
Fragments arranged by size:
4171 919 860
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 5918
AlwNI CAG_nnn'CTG
Cuts at: 2098 3693 5265 2098
Size: 1595 1572 2783
Fragments arranged by size:
2783 1595 1572
ApaI G_GGCC'C
Cuts at: 2128 3219 2128
Size: 1091 4859
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 3275 1870
Size: 73 1332 4545
Fragments arranged by size:
4545 1332 73
AvrII C'CTAG_G
Cuts at: 2842 2842
Size: 5950
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3271 4726 618
Size: 325 217 627 1484 1455 1842
Fragments arranged by size:
1842 1484 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2128 2601 2853 3219 3269 1391
Size: 737 473 252 366 50 4072
Fragments arranged by size:
4072 737 473 366 252 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3067 3174 928
Size: 971 1168 107 3704
Fragments arranged by size:
3704 1168 971 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3190 3842 5052 3190
Size: 652 1210 4088
Fragments arranged by size:
4088 1210 652
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4561 136
Size: 107 122 71 631 605 2889 1525
Fragments arranged by size:
2889 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 2484 3367 2484
Size: 883 5067
Bme1580I G_kGCm'C
Cuts at: 1790 2128 3219 4011 5167 1790
Size: 338 1091 792 1156 2573
Fragments arranged by size:
2573 1156 1091 792 338
BmgBI CAC'GTC
Cuts at: 1318 2572 2770 2983 1318
Size: 1254 198 213 4285
Fragments arranged by size:
4285 1254 213 198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2122 3681 4639
Size: 416 53 83 399 24 705 1559 958
Cuts at: 4639 442
Size: 1753
Fragments arranged by size:
1753 1559 958 705 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2972 4610 1239
Size: 1253 480 1638 2579
Fragments arranged by size:
2579 1638 1253 480
Bpu10I CC'TnA_GC
Cuts at: 2420 2420
Size: 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4613 882
Size: 617 3114 2219
Fragments arranged by size:
3114 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2715 3741 493
Size: 711 1511 1026 2702
Fragments arranged by size:
2702 1511 1026 711
BsaBI GATnn'nnATC
Cuts at: 2105 5944 2105
Size: 3839 2111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3072 3757 4139 4774
Size: 53 83 186 936 1539 685 382 635
Cuts at: 4774 275
Size: 1451
Fragments arranged by size:
1539 1451 936 685 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2216 4383 4781 5055 5202 5613 2216
Size: 2167 398 274 147 411 2553
Fragments arranged by size:
2553 2167 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2561 2591 1178
Size: 30 165 30 1158 30 4537
Fragments arranged by size:
4537 1158 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2133 2480 3146 3164 3167 3170 3173 3176
Size: 947 347 666 18 3 3 3 3
Cuts at: 3176 1186
Size: 3960
Fragments arranged by size:
3960 947 666 347 18 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2173 2601 3179 3212 5153 5649 2173
Size: 428 578 33 1941 496 2474
Fragments arranged by size:
2474 1941 578 496 428 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5377
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2870 4011 4096 5167 2870
Size: 1141 85 1071 3653
Fragments arranged by size:
3653 1141 1071 85
BsmI GAATG_Cn'
Cuts at: 2667 3461 3554 2667
Size: 794 93 5063
Fragments arranged by size:
5063 794 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2601 2853 2870 3219 3269 4011
Size: 399 338 473 252 17 349 50 742
Cuts at: 4011 4096 5167 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 473 399 349 338 252
85 50 17
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 3305 844
Size: 1376 1085 3489
Fragments arranged by size:
3489 1376 1085
BsrBI CCG'CTC
Cuts at: 1394 1823 2476 3837 1394
Size: 429 653 1361 3507
Fragments arranged by size:
3507 1361 653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2446 2835 4445 4619 65
Size: 1183 1198 389 1610 174 1396
Fragments arranged by size:
1610 1396 1198 1183 389 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2216 2373 2870 4594 4781 1700
Size: 110 406 157 497 1724 187 2869
Fragments arranged by size:
2869 1724 497 406 187 157 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 2915 2917 1925
Size: 990 2 4958
Fragments arranged by size:
4958 990 2
BssSI C'ACGA_G
Cuts at: 3014 3201 4010 5022 3014
Size: 187 809 1012 3942
Fragments arranged by size:
3942 1012 809 187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 2071 2293 1184
Size: 887 222 4841
Fragments arranged by size:
4841 887 222
BstYI r'GATC_y
Cuts at: 1972 2704 3280 3647 4032 4049 5501 5587 5599
Size: 732 576 367 385 17 1452 86 12
Cuts at: 5599 5945 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 732 576 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2220 487
Size: 683 56 994 4217
Fragments arranged by size:
4217 994 683 56
BtsI GCAGTG_nn'
Cuts at: 3060 3466 4260 4280 3060
Size: 406 794 20 4730
Fragments arranged by size:
4730 794 406 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3397 4286 5915
Size: 54 1425 50 275 18 1567 889 1629
Cuts at: 5915 8
Size: 43
Fragments arranged by size:
1629 1567 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 3880 1353
Size: 675 1852 3423
Fragments arranged by size:
3423 1852 675
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2336 4537 4911 5057 387
Size: 1064 138 747 2201 374 146 1280
Fragments arranged by size:
2201 1280 1064 747 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2492 2819 2972 4013 4610 5397 5665
Size: 393 860 327 153 1041 597 787 268
Cuts at: 5665 1239
Size: 1524
Fragments arranged by size:
1524 1041 860 787 597 393 327 268
153
EcoNI CCTnn'n_nnAGG
Cuts at: 2081 3125 2081
Size: 1044 4906
EcoO109I rG'GnC_Cy
Cuts at: 2124 3216 2124
Size: 1092 4858
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 2939 4456 2939
Size: 1517 4433
HaeII r_GCGC'y
Cuts at: 1678 3013 5097 1678
Size: 1335 2084 2531
Fragments arranged by size:
2531 2084 1335
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2561 2593 2771 2803 4591
Size: 32 135 32 989 32 178 32 1788
Cuts at: 4591 4623 4665 4697 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1788 989 178 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5064 5248 1305
Size: 3759 184 2007
Fragments arranged by size:
3759 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4128
Fragments arranged by size:
4128 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3908 4267 4426 518
Size: 648 2742 359 159 2042
Fragments arranged by size:
2742 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2201 2483 2609 2832 3004 3179 3366 4043
Size: 595 282 126 223 172 175 187 677
Cuts at: 4043 5191 5436 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 595 282 245 223 187
175 172 126
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5398
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2341 3308 4853 1132
Size: 97 1112 967 1545 2229
Fragments arranged by size:
2229 1545 1112 967 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PfoI T'CCnGG_A
Cuts at: 2733 2985 2733
Size: 252 5698
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 2124 3215 2124
Size: 1091 4859
PstI C_TGCA'G
Cuts at: 830 2082 2319 3009 3302 830
Size: 1252 237 690 293 3478
Fragments arranged by size:
3478 1252 690 293 237
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2201 2609 2832 3004 3179 2201
Size: 408 223 172 175 4972
Fragments arranged by size:
4972 408 223 175 172
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 2132 3035 4198 5910 2132
Size: 903 1163 1712 2172
Fragments arranged by size:
2172 1712 1163 903
SfcI C'TryA_G
Cuts at: 826 1045 2078 2315 3005 3298 4433 5114 5305
Size: 219 1033 237 690 293 1135 681 191
Cuts at: 5305 826
Size: 1471
Fragments arranged by size:
1471 1135 1033 690 681 293 237 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 2216 1700
Size: 516 5434
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2181 2842 3219 3389 513
Size: 552 1116 661 377 170 3074
Fragments arranged by size:
3074 1116 661 552 377 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3977 3994 4147 4332 4777 325
Size: 1255 2397 17 153 185 445 1498
Fragments arranged by size:
2397 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 3033 4196
Size: 274 80 33 51 1443 153 903 1163
Cuts at: 4196 5908 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1163 903 274 153 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3073 3758 276
Size: 53 83 186 936 1539 685 2468
Fragments arranged by size:
2468 1539 936 685 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI