Restriction map for
pFN21AB5326
(Circular) (Six-base) MAPSORT of: pFN21AB5326.seq Check: 6653 from: 1 to: 6055
pFN21AB5326 6055 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2139 3398 1937
Size: 202 1259 4594
Fragments arranged by size:
4594 1259 202
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 4118 5502 5770 1632
Size: 986 1500 1384 268 1917
Fragments arranged by size:
1917 1500 1384 986 268
AfeI AGC'GCT
Cuts at: 2622 2622
Size: 6055
AflII C'TTAA_G
Cuts at: 820 1017 2672 820
Size: 197 1655 4203
Fragments arranged by size:
4203 1655 197
AflIII A'CryG_T
Cuts at: 1203 4954 5931 1203
Size: 3751 977 1327
Fragments arranged by size:
3751 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 2682 1166
Size: 1516 4539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AlwNI CAG_nnn'CTG
Cuts at: 2078 3798 5370 2078
Size: 1720 1572 2763
Fragments arranged by size:
2763 1720 1572
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2330 2408 3197 3312 3364 3590 3742 5851
Size: 837 78 789 115 52 226 152 2109
Cuts at: 5851 1493
Size: 1697
Fragments arranged by size:
2109 1697 837 789 226 152 115 78
52
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 3380 1870
Size: 73 84 1353 4545
Fragments arranged by size:
4545 1353 84 73
BamHI G'GATC_C
Cuts at: 2443 3385 3752 2443
Size: 942 367 4746
Fragments arranged by size:
4746 942 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2047 3376 4831 618
Size: 325 217 627 227 33 1329 1455 1842
Fragments arranged by size:
1842 1455 1329 627 325 227 217 33
BanII G_rGCy'C
Cuts at: 1391 2069 2132 3374 1391
Size: 678 63 1242 4072
Fragments arranged by size:
4072 1242 678 63
BbeI G_GCGC'C
Cuts at: 2051 2051
Size: 6055
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5084
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4246 4280 1903
Size: 34 2309 34 3678
Fragments arranged by size:
3678 2309 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BfrBI ATG'CAT
Cuts at: 2864 2864
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4666 136
Size: 107 122 71 631 605 2994 1525
Fragments arranged by size:
2994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 4116 5272 1790
Size: 2326 1156 2573
Fragments arranged by size:
2573 2326 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2595 3054 3786
Size: 416 53 83 399 24 1178 459 732
Cuts at: 3786 4744 442
Size: 958 1753
Fragments arranged by size:
1753 1178 958 732 459 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2024 2593 3090 4715 1239
Size: 785 569 497 1625 2579
Fragments arranged by size:
2579 1625 785 569 497
Bpu10I CC'TnA_GC
Cuts at: 3000 3333 3000
Size: 333 5722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2512 4180 5045 5343 2512
Size: 1668 865 298 3224
Fragments arranged by size:
3224 1668 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4718 882
Size: 617 3219 2219
Fragments arranged by size:
3219 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3846 493
Size: 711 2642 2702
Fragments arranged by size:
2702 2642 711
BsaBI GATnn'nnATC
Cuts at: 2180 6049 2180
Size: 3869 2186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 3862 4244 4879
Size: 53 83 186 936 515 1814 382 635
Cuts at: 4879 275
Size: 1451
Fragments arranged by size:
1814 1451 936 635 515 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2020 4488 4886 5160 5307 5718 2020
Size: 2468 398 274 147 411 2357
Fragments arranged by size:
2468 2357 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5830
Fragments arranged by size:
5830 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6055
BseYI C'CCAG_C
Cuts at: 2062 2078 2083 5258 5754 2062
Size: 16 5 3175 496 2363
Fragments arranged by size:
3175 2363 496 16 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2977 1163
Size: 573 1241 4241
Fragments arranged by size:
4241 1241 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2097 3505 4266 5294 1540
Size: 275 186 96 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186 96
BsiHKAI G_wGCw'C
Cuts at: 2132 4116 4201 5272 2132
Size: 1984 85 1071 2915
Fragments arranged by size:
2915 1984 1071 85
BsmI GAATG_Cn'
Cuts at: 3344 3566 3659 3344
Size: 222 93 5740
Fragments arranged by size:
5740 222 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2132 3374 4116 4201 5272 1391
Size: 399 279 63 1242 742 85 1071 2174
Fragments arranged by size:
2174 1242 1071 742 399 279 85 63
BspEI T'CCGG_A
Cuts at: 2020 5718 2020
Size: 3698 2357
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3410 844
Size: 2566 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 3942 1394
Size: 429 209 1910 3507
Fragments arranged by size:
3507 1910 429 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 2942 4550 4724 65
Size: 1183 1694 1608 174 1396
Fragments arranged by size:
1694 1608 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 4699 4886 1700
Size: 110 234 2655 187 2869
Fragments arranged by size:
2869 2655 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 2443 3385 3752 4137 4154 5606 5692 5704
Size: 471 942 367 385 17 1452 86 12
Cuts at: 5704 6050 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 942 471 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4564
Fragments arranged by size:
4564 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 3032 3571 4365 4385 3032
Size: 539 794 20 4702
Fragments arranged by size:
4702 794 539 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3502 4391 6020
Size: 54 1425 50 275 18 1672 889 1629
Cuts at: 6020 8
Size: 43
Fragments arranged by size:
1672 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 3985 1353
Size: 765 1867 3423
Fragments arranged by size:
3423 1867 765
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 5016 5162 387
Size: 1064 138 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2130 2130
Size: 6055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2593 2618 3090 4118 4715 5502
Size: 393 392 569 25 472 1028 597 787
Cuts at: 5502 5770 1239
Size: 268 1524
Fragments arranged by size:
1524 1028 787 597 569 472 393 392
268 25
EcoO109I rG'GnC_Cy
Cuts at: 3265 3265
Size: 6055
EcoRI G'AATT_C
Cuts at: 3312 3364 3742 3312
Size: 52 378 5625
Fragments arranged by size:
5625 378 52
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2942 3358 2942
Size: 416 5639
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 2051 2624 5202 1678
Size: 373 573 2578 2531
Fragments arranged by size:
2578 2531 573 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2514 2546 2827 2859 2983
Size: 32 135 32 942 32 281 32 124
Cuts at: 2983 2996 3015 3028 4696 4728 4770 4802 1373
Size: 13 19 13 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 942 281 135 124 42 32
32 32 32 32 32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2140 3399 3645 669
Size: 988 281 202 1259 246 3079
Fragments arranged by size:
3079 1259 988 281 246 202
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KasI G'GCGC_C
Cuts at: 2047 2047
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5169 5353 1305
Size: 3864 184 2007
Fragments arranged by size:
3864 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4233
Fragments arranged by size:
4233 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 2283 2682 2853 4013 4372 4531
Size: 648 1062 55 399 171 1160 359 159
Cuts at: 4531 518
Size: 2042
Fragments arranged by size:
2042 1160 1062 648 399 359 171 159
55
MspA1I CmG'CkG
Cuts at: 1606 2062 2596 3471 4148 5296 5541 1606
Size: 456 534 875 677 1148 245 2120
Fragments arranged by size:
2120 1148 875 677 534 456 245
NaeI GCC'GGC
Cuts at: 2046 2046
Size: 6055
NarI GG'CG_CC
Cuts at: 2048 2048
Size: 6055
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4564
Fragments arranged by size:
4564 939 552
NdeI CA'TA_TG
Cuts at: 387 2850 387
Size: 2463 3592
NgoMIV G'CCGG_C
Cuts at: 2044 2044
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NsiI A_TGCA'T
Cuts at: 2866 2866
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 2233 3413 4958 1132
Size: 97 1004 1180 1545 2229
Fragments arranged by size:
2229 1545 1180 1004 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2078 1108
Size: 970 5085
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PstI C_TGCA'G
Cuts at: 830 2697 2835 3407 830
Size: 1867 138 572 3478
Fragments arranged by size:
3478 1867 572 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2596 2596
Size: 6055
SacI G_AGCT'C
Cuts at: 2132 2132
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 2138 3397 1936
Size: 202 1259 4594
Fragments arranged by size:
4594 1259 202
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2680 2693 2831 3403 4538 5219 5410
Size: 219 1635 13 138 572 1135 681 191
Cuts at: 5410 826
Size: 1471
Fragments arranged by size:
1635 1471 1135 681 572 219 191 138
13
SfoI GGC'GCC
Cuts at: 2049 2049
Size: 6055
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2491 2672 4159 5060 5322 820
Size: 197 926 548 181 1487 901 262 1553
Fragments arranged by size:
1553 1487 926 901 548 262 197 181
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 3494 513
Size: 552 939 48 1442 3074
Fragments arranged by size:
3074 1442 939 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2810 4082 4099 4252 4437 4882 325
Size: 1255 1230 1272 17 153 185 445 1498
Fragments arranged by size:
1498 1272 1255 1230 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2764 4301 6013
Size: 274 80 33 51 1443 787 1537 1712
Cuts at: 6013 96
Size: 138
Fragments arranged by size:
1712 1537 1443 787 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2496 2528 6015 6047 2496
Size: 32 3487 32 2504
Fragments arranged by size:
3487 2504 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 2484 4184 2484
Size: 1700 4355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI