Restriction map for
pFN21AB5324
(Circular) (Six-base) MAPSORT of: pFN21AB5324.seq Check: 6281 from: 1 to: 6085
pFN21AB5324 6085 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2032 2032
Size: 6085
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3895 278
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3428 1937
Size: 1491 4594
Acc65I G'GTAC_C
Cuts at: 2228 3406 2228
Size: 1178 4907
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1998 2663 4148 5532 5800 1632
Size: 366 665 1485 1384 268 1917
Fragments arranged by size:
1917 1485 1384 665 366 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 4984 5961 1203
Size: 3781 977 1327
Fragments arranged by size:
3781 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 4814 4814
Size: 6085
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6085
AlwNI CAG_nnn'CTG
Cuts at: 2503 2747 3828 5400 2503
Size: 244 1081 1572 3188
Fragments arranged by size:
3188 1572 1081 244
ApaI G_GGCC'C
Cuts at: 2479 2800 2479
Size: 321 5764
ApaLI G'TGCA_C
Cuts at: 2617 3359 4142 5298 2617
Size: 742 783 1156 3404
Fragments arranged by size:
3404 1156 783 742
ApoI r'AATT_y
Cuts at: 1493 3394 3620 3772 5881 1493
Size: 1901 226 152 2109 1697
Fragments arranged by size:
2109 1901 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 3191 3410 1870
Size: 73 987 261 219 4545
Fragments arranged by size:
4545 987 261 219 73
BamHI G'GATC_C
Cuts at: 3415 3782 3415
Size: 367 5718
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 2228 3406 4861 618
Size: 325 217 627 266 175 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 325 266 217 175
BanII G_rGCy'C
Cuts at: 1391 2172 2281 2479 2687 2756 2800 2969 3404
Size: 781 109 198 208 69 44 169 435
Cuts at: 3404 1391
Size: 4072
Fragments arranged by size:
4072 781 435 208 198 169 109 69
44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3068 928
Size: 971 1169 3945
Fragments arranged by size:
3945 1169 971
BbvCI CC'TCA_GC
Cuts at: 2748 2748
Size: 6085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 1903
Size: 34 2339 34 3678
Fragments arranged by size:
3678 2339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 2907 1548
Size: 1359 4726
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4696 136
Size: 107 122 71 631 605 3024 1525
Fragments arranged by size:
3024 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6080 6080
Size: 6085
BlpI GC'TnA_GC
Cuts at: 2198 3502 2198
Size: 1304 4781
Bme1580I G_kGCm'C
Cuts at: 1790 2288 2479 2621 2800 3363 4146 5302 1790
Size: 498 191 142 179 563 783 1156 2573
Fragments arranged by size:
2573 1156 783 563 498 191 179 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2284 3816 4774
Size: 416 53 83 399 24 867 1532 958
Cuts at: 4774 442
Size: 1753
Fragments arranged by size:
1753 1532 958 867 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2117 2528 1056
Size: 1061 411 4613
Fragments arranged by size:
4613 1061 411
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2754 4745 1239
Size: 1515 1991 2579
Fragments arranged by size:
2579 1991 1515
Bpu10I CC'TnA_GC
Cuts at: 2396 2748 2396
Size: 352 5733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 4210 5075 5373 2156
Size: 2054 865 298 2868
Fragments arranged by size:
2868 2054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2739 3188 4748 882
Size: 617 1240 449 1560 2219
Fragments arranged by size:
2219 1560 1240 617 449
BsaAI yAC'GTr
Cuts at: 493 1204 2256 3876 493
Size: 711 1052 1620 2702
Fragments arranged by size:
2702 1620 1052 711
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2693 3892 4274 4909
Size: 53 83 186 936 1160 1199 382 635
Cuts at: 4909 275
Size: 1451
Fragments arranged by size:
1451 1199 1160 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4518 4916 5190 5337 5748 4518
Size: 398 274 147 411 4855
Fragments arranged by size:
4855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2837 2867 1178
Size: 30 165 30 1434 30 4396
Fragments arranged by size:
4396 1434 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2757 2900 1186
Size: 1571 143 4371
Fragments arranged by size:
4371 1571 143
BseYI C'CCAG_C
Cuts at: 2479 2528 2556 3208 3312 5288 5784 2479
Size: 49 28 652 104 1976 496 2780
Fragments arranged by size:
2780 1976 652 496 104 49 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5512
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3535 4296 5324 1540
Size: 275 186 1534 761 1028 2301
Fragments arranged by size:
2301 1534 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2472 2621 2662 2969 3363 4146 4231 5302
Size: 300 149 41 307 394 783 85 1071
Cuts at: 5302 2172
Size: 2955
Fragments arranged by size:
2955 1071 783 394 307 300 149 85
41
BsmI GAATG_Cn'
Cuts at: 3596 3689 3596
Size: 93 5992
BsmBI CGTCTCn'nnnn_
Cuts at: 2684 3316 2684
Size: 632 5453
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2281 2288 2472 2479 2621 2662
Size: 399 382 109 7 184 7 142 41
Cuts at: 2662 2687 2756 2800 2969 3363 3404 4146 4231
Size: 25 69 44 169 394 41 742 85
Cuts at: 4231 5302 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 382 184 169
142 109 85 69 44 41 41 25
7 7
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 2343 3974 5704 2343
Size: 1631 1730 2724
Fragments arranged by size:
2724 1730 1631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2032 2142 2268 3440 844
Size: 1188 110 126 1172 3489
Fragments arranged by size:
3489 1188 1172 126 110
BsrBI CCG'CTC
Cuts at: 1394 1823 2515 3972 1394
Size: 429 692 1457 3507
Fragments arranged by size:
3507 1457 692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2338 4580 4754 65
Size: 1183 1090 2242 174 1396
Fragments arranged by size:
2242 1396 1183 1090 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4729 4916 1700
Size: 110 2919 187 2869
Fragments arranged by size:
2919 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 4145 5157 4145
Size: 1012 5073
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstXI CCAn_nnnn'nTGG
Cuts at: 3118 3118
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 3336 3415 3782 4167 4184 5636 5722 5734
Size: 1364 79 367 385 17 1452 86 12
Cuts at: 5734 6080 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1364 385 367 346 86 79
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2097 2583 2856 2960 3034 513
Size: 552 1032 486 273 104 74 3564
Fragments arranged by size:
3564 1032 552 486 273 104 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5346
Fragments arranged by size:
5346 683 56
BtsI GCAGTG_nn'
Cuts at: 2540 3601 4395 4415 2540
Size: 1061 794 20 4210
Fragments arranged by size:
4210 1061 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2689 2724 529
Size: 35 2125 35 3890
Fragments arranged by size:
3890 2125 35 35
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5092 809
Size: 507 3776 1802
Fragments arranged by size:
3776 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 3051 3532
Size: 54 1425 50 275 18 495 726 481
Cuts at: 3532 4421 6050 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 726 495 481 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3532 1537
Size: 275 1720 4090
Fragments arranged by size:
4090 1720 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2495 2829 2835 4015 1353
Size: 1142 334 6 1180 3423
Fragments arranged by size:
3423 1180 1142 334 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4672 5046 5192 387
Size: 1064 138 3083 374 146 1280
Fragments arranged by size:
3083 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2170 2967 2170
Size: 797 5288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2663 2754 4148 4745 5532 5800
Size: 393 366 665 91 1394 597 787 268
Cuts at: 5800 1239
Size: 1524
Fragments arranged by size:
1524 1394 787 665 597 393 366 268
91
EcoO109I rG'GnC_Cy
Cuts at: 2066 2476 2797 2941 2066
Size: 410 321 144 5210
Fragments arranged by size:
5210 410 321 144
EcoRI G'AATT_C
Cuts at: 3394 3772 3394
Size: 378 5707
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 3349 5232 1678
Size: 1671 1883 2531
Fragments arranged by size:
2531 1883 1671
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2837 2869 4726 4758 4800
Size: 32 135 32 1265 32 1857 32 42
Cuts at: 4800 4832 1373
Size: 32 2626
Fragments arranged by size:
2626 1857 1265 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 3232 3429 3675 669
Size: 988 281 223 1071 197 246 3079
Fragments arranged by size:
3079 1071 988 281 246 223 197
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6085
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KpnI G_GTAC'C
Cuts at: 2232 3410 2232
Size: 1178 4907
MfeI C'AATT_G
Cuts at: 3684 3684
Size: 6085
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5199 5383 1305
Size: 3894 184 2007
Fragments arranged by size:
3894 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 3053 10
Size: 54 1425 343 495 726 3042
Fragments arranged by size:
3042 1425 726 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 2612 3155 4043 4402 4561 518
Size: 648 1416 30 543 888 359 159 2042
Fragments arranged by size:
2042 1416 888 648 543 359 159 30
MspA1I CmG'CkG
Cuts at: 1606 2778 2823 3049 3312 3501 4178 5326 5571
Size: 1172 45 226 263 189 677 1148 245
Cuts at: 5571 1606
Size: 2120
Fragments arranged by size:
2120 1172 1148 677 263 245 226 189
45
NcoI C'CATG_G
Cuts at: 513 1065 2583 2856 513
Size: 552 1518 273 3742
Fragments arranged by size:
3742 1518 552 273
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6085
NheI G'CTAG_C
Cuts at: 1052 2113 2524 1052
Size: 1061 411 4613
Fragments arranged by size:
4613 1061 411
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 3443 4988 1132
Size: 97 2214 1545 2229
Fragments arranged by size:
2229 2214 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6085
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6085
PfoI T'CCnGG_A
Cuts at: 3279 3279
Size: 6085
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 2066
Size: 6085
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6085
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PspOMI G'GGCC_C
Cuts at: 2475 2796 2475
Size: 321 5764
PstI C_TGCA'G
Cuts at: 830 2814 3437 830
Size: 1984 623 3478
Fragments arranged by size:
3478 1984 623
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
PvuII CAG'CTG
Cuts at: 2823 3049 2823
Size: 226 5859
SacI G_AGCT'C
Cuts at: 2172 2969 2172
Size: 797 5288
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SanDI GG'GwC_CC
Cuts at: 2066 2066
Size: 6085
SbfI CC_TGCA'GG
Cuts at: 3437 3437
Size: 6085
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SexAI A'CCwGG_T
Cuts at: 2502 2502
Size: 6085
SfcI C'TryA_G
Cuts at: 826 1045 2810 2818 3433 4568 5249 5440 826
Size: 219 1765 8 615 1135 681 191 1471
Fragments arranged by size:
1765 1471 1135 681 615 219 191 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 3412 1872
Size: 1540 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 4189 5090 5352 820
Size: 197 926 228 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 228 197
SnaBI TAC'GTA
Cuts at: 493 3876 493
Size: 3383 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 4009 5
Size: 47 3957 2081
Fragments arranged by size:
3957 2081 47
StuI AGG'CCT
Cuts at: 1517 2816 1517
Size: 1299 4786
StyI C'CwwG_G
Cuts at: 513 1065 2583 2856 3524 513
Size: 552 1518 273 668 3074
Fragments arranged by size:
3074 1518 668 552 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4112 4129 4282 4467 4912 325
Size: 1255 2532 17 153 185 445 1498
Fragments arranged by size:
2532 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4331 6043 96
Size: 274 80 33 51 1443 2354 1712 138
Fragments arranged by size:
2354 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3091 3123 6045 6077 3091
Size: 32 2922 32 3099
Fragments arranged by size:
3099 2922 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6085
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3032 3032
Size: 6085
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 3410 1870
Size: 1540 4545
XmnI GAAnn'nnTTC
Cuts at: 3065 4214 3065
Size: 1149 4936
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3893 276
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI
SwaI