Restriction map for
pFN21AB5317
(Circular) (Six-base) MAPSORT of: pFN21AB5317.seq Check: 8432 from: 1 to: 5881
pFN21AB5317 5881 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 2932 3944 5328 5596 1632
Size: 656 644 1012 1384 268 1917
Fragments arranged by size:
1917 1384 1012 656 644 268
AfeI AGC'GCT
Cuts at: 2945 2945
Size: 5881
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 2682 4780 5757 1203
Size: 1479 2098 977 1327
Fragments arranged by size:
2098 1479 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 2546 2764 3014 3028 3091 3624 5196 2546
Size: 218 250 14 63 533 1572 3231
Fragments arranged by size:
3231 1572 533 250 218 63 14
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 2146 2198 3190 3416 3568 5677 1493
Size: 653 52 992 226 152 2109 1697
Fragments arranged by size:
2109 1697 992 653 226 152 52
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 3206 1870
Size: 73 174 1089 4545
Fragments arranged by size:
4545 1089 174 73
BamHI G'GATC_C
Cuts at: 2371 3211 3578 2371
Size: 840 367 4674
Fragments arranged by size:
4674 840 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2726 3133 3202 4657 618
Size: 325 217 627 939 407 69 1455 1842
Fragments arranged by size:
1842 1455 939 627 407 325 217 69
BanII G_rGCy'C
Cuts at: 1391 3200 1391
Size: 1809 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2367 3084 928
Size: 971 468 717 3725
Fragments arranged by size:
3725 971 717 468
BbvCI CC'TCA_GC
Cuts at: 2675 2675
Size: 5881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2040 2074 4072 4106 1903
Size: 34 103 34 1998 34 3678
Fragments arranged by size:
3678 1998 103 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4492 136
Size: 107 122 71 631 605 337 2483 1525
Fragments arranged by size:
2483 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 2894 3298 2894
Size: 404 5477
Bme1580I G_kGCm'C
Cuts at: 1790 3942 5098 1790
Size: 2152 1156 2573
Fragments arranged by size:
2573 2152 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2080 3612 4570
Size: 416 53 83 399 24 663 1532 958
Cuts at: 4570 442
Size: 1753
Fragments arranged by size:
1753 1532 958 663 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2386 2804 4541 1239
Size: 1147 418 1737 2579
Fragments arranged by size:
2579 1737 1147 418
Bpu10I CC'TnA_GC
Cuts at: 2675 2675
Size: 5881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2162 2924 4006 4871 5169 2162
Size: 762 1082 865 298 2874
Fragments arranged by size:
2874 1082 865 762 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2291 4544 882
Size: 617 792 2253 2219
Fragments arranged by size:
2253 2219 792 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 5875 5875
Size: 5881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 4070 4705 275
Size: 53 83 186 936 2155 382 635 1451
Fragments arranged by size:
2155 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2465 4314 4712 4986 5133 5544 2465
Size: 1849 398 274 147 411 2802
Fragments arranged by size:
2802 1849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5656
Fragments arranged by size:
5656 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2303 2306 2309 2327 2492 2525 2573
Size: 904 213 3 3 18 165 33 48
Cuts at: 2573 2598 2762 2995 3054 1186
Size: 25 164 233 59 4013
Fragments arranged by size:
4013 904 233 213 165 164 59 48
33 25 18 3 3
BseYI C'CCAG_C
Cuts at: 5084 5580 5084
Size: 496 5385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2777 1163
Size: 573 1041 4267
Fragments arranged by size:
4267 1041 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3171 3942 4027 5098 3171
Size: 771 85 1071 3954
Fragments arranged by size:
3954 1071 771 85
BsmI GAATG_Cn'
Cuts at: 3392 3485 3392
Size: 93 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3171 3200 3942 4027 5098 1391
Size: 399 1381 29 742 85 1071 2174
Fragments arranged by size:
2174 1381 1071 742 399 85 29
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3090 3236 844
Size: 2246 146 3489
Fragments arranged by size:
3489 2246 146
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4376 4550 65
Size: 1183 3128 174 1396
Fragments arranged by size:
3128 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4525 4712 1700
Size: 110 2715 187 2869
Fragments arranged by size:
2869 2715 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 3941 4953 3941
Size: 1012 4869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2626 794
Size: 1832 4049
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstXI CCAn_nnnn'nTGG
Cuts at: 2999 2999
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 2280 2371 2415 3211 3578 3963 3980 5432
Size: 308 91 44 796 367 385 17 1452
Cuts at: 5432 5518 5530 5876 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 796 385 367 346 308 91
86 44 17 12
Bsu36I CC'TnA_GG
Cuts at: 2547 3007 2547
Size: 460 5421
BtgI C'CryG_G
Cuts at: 513 1065 2004 2178 513
Size: 552 939 174 4216
Fragments arranged by size:
4216 939 552 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 3397 4191 4211 3397
Size: 794 20 5067
Fragments arranged by size:
5067 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3162 3185 3162
Size: 23 5858
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846
Size: 54 1425 50 275 18 1498 889 1629
Cuts at: 5846 8
Size: 43
Fragments arranged by size:
1629 1498 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 2424 2430 2444 2550 2568 3811 1353
Size: 675 396 6 14 106 18 1243 3423
Fragments arranged by size:
3423 1243 675 396 106 18 14 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2893 2978 4468 4842 4988 387
Size: 1064 138 1304 85 1490 374 146 1280
Fragments arranged by size:
1490 1304 1280 1064 374 146 138 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2386 2804 2932 3944 4541 5328
Size: 393 656 98 418 128 1012 597 787
Cuts at: 5328 5596 1239
Size: 268 1524
Fragments arranged by size:
1524 1012 787 656 597 418 393 268
128 98
EcoNI CCTnn'n_nnAGG
Cuts at: 2618 2618
Size: 5881
EcoO109I rG'GnC_Cy
Cuts at: 2011 2044 2068 2122 2185 2011
Size: 33 24 54 63 5707
Fragments arranged by size:
5707 63 54 33 24
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 5881
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 2947 5028 1678
Size: 1269 2081 2531
Fragments arranged by size:
2531 2081 1269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4522 4554 4596 4628 1373
Size: 32 135 32 2950 32 42 32 2626
Fragments arranged by size:
2950 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5881
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2991 4995 5179 1305
Size: 1686 2004 184 2007
Fragments arranged by size:
2007 2004 1686 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3839 4198 4357 518
Size: 648 2673 359 159 2042
Fragments arranged by size:
2673 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2764 3297 3974 5122 5367 1606
Size: 1158 533 677 1148 245 2120
Fragments arranged by size:
2120 1158 1148 677 533 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2178 513
Size: 552 939 174 4216
Fragments arranged by size:
4216 939 552 174
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 2686 3239 4784 1132
Size: 97 1457 553 1545 2229
Fragments arranged by size:
2229 1545 1457 553 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 2682 4780 2682
Size: 2098 3783
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2423 2455 3950 3982 5463 5495 2423
Size: 32 1495 32 1481 32 2809
Fragments arranged by size:
2809 1495 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 2068 2122 2185 2044
Size: 24 54 63 5740
Fragments arranged by size:
5740 63 54 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PstI C_TGCA'G
Cuts at: 830 2622 2904 2979 3087 3233 830
Size: 1792 282 75 108 146 3478
Fragments arranged by size:
3478 1792 282 146 108 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2764 2764
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SbfI CC_TGCA'GG
Cuts at: 2622 3233 2622
Size: 611 5270
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SexAI A'CCwGG_T
Cuts at: 2715 2715
Size: 5881
SfcI C'TryA_G
Cuts at: 826 1045 2618 2900 2975 3083 3229 4364 5045
Size: 219 1573 282 75 108 146 1135 681
Cuts at: 5045 5236 826
Size: 191 1471
Fragments arranged by size:
1573 1471 1135 681 282 219 191 146
108 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3208 1872
Size: 1336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 2141 2939 3985 4886 5148
Size: 197 926 174 24 798 1046 901 262
Cuts at: 5148 820
Size: 1553
Fragments arranged by size:
1553 1046 926 901 798 262 197 174
24
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2004 2178 3320 513
Size: 552 939 174 1142 3074
Fragments arranged by size:
3074 1142 939 552 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 2599 2717 3908 3925 4078 4263
Size: 1255 681 338 118 1191 17 153 185
Cuts at: 4263 4708 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1191 681 445 338 185 153
118 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4127 5839 96
Size: 274 80 33 51 1443 2150 1712 138
Fragments arranged by size:
2150 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3025 3057 5841 5873 3025
Size: 32 2784 32 3033
Fragments arranged by size:
3033 2784 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2609 1316
Size: 1293 4588
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2093 2093
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 2117 1943
Size: 174 5707
XmaI C'CCGG_G
Cuts at: 1870 3206 1870
Size: 1336 4545
XmnI GAAnn'nnTTC
Cuts at: 4010 4010
Size: 5881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI