Restriction map for
pFN21AB5316
(Circular) (Six-base) MAPSORT of: pFN21AB5316.seq Check: 9405 from: 1 to: 6055
pFN21AB5316 6055 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 4118 5502 5770 1632
Size: 456 2030 1384 268 1917
Fragments arranged by size:
2030 1917 1384 456 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 4954 5931 1203
Size: 3751 977 1327
Fragments arranged by size:
3751 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AlwNI CAG_nnn'CTG
Cuts at: 3798 5370 3798
Size: 1572 4483
ApaLI G'TGCA_C
Cuts at: 3279 4112 5268 3279
Size: 833 1156 4066
Fragments arranged by size:
4066 1156 833
ApoI r'AATT_y
Cuts at: 1493 2377 2414 2535 2628 2774 2946 2962 2999
Size: 884 37 121 93 146 172 16 37
Cuts at: 2999 3319 3364 3590 3742 5851 1493
Size: 320 45 226 152 2109 1697
Fragments arranged by size:
2109 1697 884 320 226 172 152 146
121 93 45 37 37 16
AseI AT'TA_AT
Cuts at: 160 1032 2340 3110 4609 160
Size: 872 1308 770 1499 1606
Fragments arranged by size:
1606 1499 1308 872 770
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 3380 1870
Size: 73 1437 4545
Fragments arranged by size:
4545 1437 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3376 4831 618
Size: 325 217 627 1589 1455 1842
Fragments arranged by size:
1842 1589 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3374 1391
Size: 1983 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5084
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2832 2866 4246 4280 1903
Size: 34 895 34 1380 34 3678
Fragments arranged by size:
3678 1380 895 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 3232 1548
Size: 1684 4371
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4666 136
Size: 107 122 71 631 605 2994 1525
Fragments arranged by size:
2994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 3283 4116 5272 1790
Size: 1493 833 1156 2573
Fragments arranged by size:
2573 1493 1156 833
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3786 4744 442
Size: 416 53 83 399 24 2369 958 1753
Fragments arranged by size:
2369 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4715 1239
Size: 3476 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2782 4180 5045 5343 2782
Size: 1398 865 298 3494
Fragments arranged by size:
3494 1398 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4718 882
Size: 617 3219 2219
Fragments arranged by size:
3219 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3846 493
Size: 711 2642 2702
Fragments arranged by size:
2702 2642 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3862 4244 4879 275
Size: 53 83 186 936 2329 382 635 1451
Fragments arranged by size:
2329 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4488 4886 5160 5307 5718 4488
Size: 398 274 147 411 4825
Fragments arranged by size:
4825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5830
Fragments arranged by size:
5830 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 1186
Size: 871 5184
BseYI C'CCAG_C
Cuts at: 5258 5754 5258
Size: 496 5559
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2328 3215 3276 1163
Size: 573 592 887 61 3942
Fragments arranged by size:
3942 887 592 573 61
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3283 4116 4201 5272 3283
Size: 833 85 1071 4066
Fragments arranged by size:
4066 1071 833 85
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3283 3374 4116 4201 5272 1391
Size: 399 1493 91 742 85 1071 2174
Fragments arranged by size:
2174 1493 1071 742 399 91 85
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3410 844
Size: 2566 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3942 1394
Size: 429 2119 3507
Fragments arranged by size:
3507 2119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2961 4550 4724 65
Size: 1183 1713 1589 174 1396
Fragments arranged by size:
1713 1589 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4699 4886 1700
Size: 110 2889 187 2869
Fragments arranged by size:
2889 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2637 96
Size: 2541 3514
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 3385 3752 4137 4154 5606 5692 5704 6050
Size: 1413 367 385 17 1452 86 12 346
Cuts at: 6050 1972
Size: 1977
Fragments arranged by size:
1977 1452 1413 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2049 487
Size: 683 56 823 4493
Fragments arranged by size:
4493 823 683 56
BtsI GCAGTG_nn'
Cuts at: 3203 3571 4365 4385 3203
Size: 368 794 20 4873
Fragments arranged by size:
4873 794 368 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 2212 3359 2212
Size: 1147 4908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3223 3502 4391
Size: 54 1425 50 275 18 1393 279 889
Cuts at: 4391 6020 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1393 889 279 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3063 3263 3985 1353
Size: 1710 200 722 3423
Fragments arranged by size:
3423 1710 722 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 5016 5162 387
Size: 1064 138 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 4118 4715 5502 5770 1239
Size: 393 456 2030 597 787 268 1524
Fragments arranged by size:
2030 1524 787 597 456 393 268
EcoRI G'AATT_C
Cuts at: 2377 2414 3364 3742 2377
Size: 37 950 378 4690
Fragments arranged by size:
4690 950 378 37
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 6055
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 5202 1678
Size: 3524 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 2345 2377 4696
Size: 32 135 32 491 32 250 32 2319
Cuts at: 4696 4728 4770 4802 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2319 491 250 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2250 3241 3399 3645 669
Size: 988 281 312 991 158 246 3079
Fragments arranged by size:
3079 991 988 312 281 246 158
HindIII A'AGCT_T
Cuts at: 748 2436 2486 2525 748
Size: 1688 50 39 4278
Fragments arranged by size:
4278 1688 50 39
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5169 5353 1305
Size: 3864 184 2007
Fragments arranged by size:
3864 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4233
Fragments arranged by size:
4233 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4013 4372 4531 518
Size: 648 2847 359 159 2042
Fragments arranged by size:
2847 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3471 4148 5296 5541 1606
Size: 1865 677 1148 245 2120
Fragments arranged by size:
2120 1865 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5503
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 3413 4958 1132
Size: 97 2184 1545 2229
Fragments arranged by size:
2229 2184 1545 97
PacI TTA_AT'TAA
Cuts at: 3114 3114
Size: 6055
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6055
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3104 3625 3104
Size: 521 5534
PstI C_TGCA'G
Cuts at: 830 2412 3407 830
Size: 1582 995 3478
Fragments arranged by size:
3478 1582 995
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SapI GCTCTTCn'nnn_
Cuts at: 3063 3063
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 2111 4303 6015 2111
Size: 2192 1712 2151
Fragments arranged by size:
2192 2151 1712
SfcI C'TryA_G
Cuts at: 826 1045 2408 3336 3403 4538 5219 5410 826
Size: 219 1363 928 67 1135 681 191 1471
Fragments arranged by size:
1471 1363 1135 928 681 219 191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2761 4159 5060 5322 820
Size: 197 926 818 1398 901 262 1553
Fragments arranged by size:
1553 1398 926 901 818 262 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 2861 3979 5
Size: 47 2809 1118 2081
Fragments arranged by size:
2809 2081 1118 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 3494 513
Size: 552 2429 3074
Fragments arranged by size:
3074 2429 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4082 4099 4252 4437 4882 325
Size: 1255 2502 17 153 185 445 1498
Fragments arranged by size:
2502 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 2637 4301
Size: 274 80 33 51 1443 132 528 1664
Cuts at: 4301 6013 96
Size: 1712 138
Fragments arranged by size:
1712 1664 1443 528 274 138 132 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6015 6047 6015
Size: 32 6023
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2570 2011
Size: 559 5496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 2323 3009 4184 2323
Size: 686 1175 4194
Fragments arranged by size:
4194 1175 686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI