Restriction map for
pFN21AB5311
(Circular) (Six-base) MAPSORT of: pFN21AB5311.seq Check: 5973 from: 1 to: 6103
pFN21AB5311 6103 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3446 1937
Size: 1509 4594
Acc65I G'GTAC_C
Cuts at: 3424 3424
Size: 6103
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 2576 4166 5550 5818 1632
Size: 650 294 1590 1384 268 1917
Fragments arranged by size:
1917 1590 1384 650 294 268
AflII C'TTAA_G
Cuts at: 820 1017 3332 820
Size: 197 2315 3591
Fragments arranged by size:
3591 2315 197
AflIII A'CryG_T
Cuts at: 1203 2142 3075 5002 5979 1203
Size: 939 933 1927 977 1327
Fragments arranged by size:
1927 1327 977 939 933
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 2375 1166
Size: 1209 4894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AlwNI CAG_nnn'CTG
Cuts at: 2108 3157 3247 3846 5418 2108
Size: 1049 90 599 1572 2793
Fragments arranged by size:
2793 1572 1049 599 90
ApaLI G'TGCA_C
Cuts at: 3101 4160 5316 3101
Size: 1059 1156 3888
Fragments arranged by size:
3888 1156 1059
ApoI r'AATT_y
Cuts at: 1493 2298 3089 3412 3638 3790 5899 1493
Size: 805 791 323 226 152 2109 1697
Fragments arranged by size:
2109 1697 805 791 323 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 2722 3428 1870
Size: 73 779 706 4545
Fragments arranged by size:
4545 779 706 73
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3083 3382 3424 4879 618
Size: 325 217 627 1296 299 42 1455 1842
Fragments arranged by size:
1842 1455 1296 627 325 299 217 42
BanII G_rGCy'C
Cuts at: 1391 2247 3150 3422 1391
Size: 856 903 272 4072
Fragments arranged by size:
4072 903 856 272
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2462 2858 928
Size: 971 563 396 4173
Fragments arranged by size:
4173 971 563 396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3165 3199 4294 4328 1903
Size: 34 1228 34 1095 34 3678
Fragments arranged by size:
3678 1228 1095 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 2794 1548
Size: 1246 4857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 136
Size: 107 122 71 631 605 3042 1525
Fragments arranged by size:
3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6098 6098
Size: 6103
BlpI GC'TnA_GC
Cuts at: 2912 3520 2912
Size: 608 5495
Bme1580I G_kGCm'C
Cuts at: 1790 3105 3224 4164 5320 1790
Size: 1315 119 940 1156 2573
Fragments arranged by size:
2573 1315 1156 940 119
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2577 3834 4792
Size: 416 53 83 399 24 1160 1257 958
Cuts at: 4792 442
Size: 1753
Fragments arranged by size:
1753 1257 1160 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 4763 1239
Size: 854 2670 2579
Fragments arranged by size:
2670 2579 854
Bpu10I CC'TnA_GC
Cuts at: 2667 2667
Size: 6103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1997 4228 5093 5391 1997
Size: 2231 865 298 2709
Fragments arranged by size:
2709 2231 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2163 4766 882
Size: 617 664 2603 2219
Fragments arranged by size:
2603 2219 664 617
BsaAI yAC'GTr
Cuts at: 493 1204 2341 3894 493
Size: 711 1137 1553 2702
Fragments arranged by size:
2702 1553 1137 711
BsaBI GATnn'nnATC
Cuts at: 3131 6097 3131
Size: 2966 3137
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3910 4292 4927 275
Size: 53 83 186 936 2377 382 635 1451
Fragments arranged by size:
2377 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2923 4536 4934 5208 5355 5766 2923
Size: 1613 398 274 147 411 3260
Fragments arranged by size:
3260 1613 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5878
Fragments arranged by size:
5878 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2623 3029 1186
Size: 1437 406 4260
Fragments arranged by size:
4260 1437 406
BseYI C'CCAG_C
Cuts at: 5306 5802 5306
Size: 496 5607
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2460 3385 1163
Size: 573 724 925 3881
Fragments arranged by size:
3881 925 724 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2963 3553 4314 5342 1540
Size: 275 186 962 590 761 1028 2301
Fragments arranged by size:
2301 1028 962 761 590 275 186
BsiHKAI G_wGCw'C
Cuts at: 3105 4164 4249 5320 3105
Size: 1059 85 1071 3888
Fragments arranged by size:
3888 1071 1059 85
BsmI GAATG_Cn'
Cuts at: 2840 2908 3614 3707 2840
Size: 68 706 93 5236
Fragments arranged by size:
5236 706 93 68
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 3105 3150 3224 3422 4164 4249
Size: 399 457 858 45 74 198 742 85
Cuts at: 4249 5320 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 858 742 457 399 198 85
74 45
BspEI T'CCGG_A
Cuts at: 2923 5766 2923
Size: 2843 3260
BspHI T'CATG_A
Cuts at: 2490 3992 5722 2490
Size: 1502 1730 2871
Fragments arranged by size:
2871 1730 1502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3458 844
Size: 2614 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3201 4598 4772 65
Size: 1183 1953 1397 174 1396
Fragments arranged by size:
1953 1397 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4747 4934 1700
Size: 110 2937 187 2869
Fragments arranged by size:
2937 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2145 96
Size: 2049 4054
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6103
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2083 3247 794
Size: 1289 1164 3650
Fragments arranged by size:
3650 1289 1164
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstXI CCAn_nnnn'nTGG
Cuts at: 2881 2881
Size: 6103
BstYI r'GATC_y
Cuts at: 1972 2352 3108 3433 3800 4185 4202 5654 5740
Size: 380 756 325 367 385 17 1452 86
Cuts at: 5740 5752 6098 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 756 385 380 367 346 325
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2247 513
Size: 552 1182 4369
Fragments arranged by size:
4369 1182 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2426 487
Size: 683 56 1200 4164
Fragments arranged by size:
4164 1200 683 56
BtsI GCAGTG_nn'
Cuts at: 3619 4413 4433 3619
Size: 794 20 5289
Fragments arranged by size:
5289 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2099 2292 2772
Size: 54 1425 50 275 18 269 193 480
Cuts at: 2772 2960 3550 4439 6068 8
Size: 188 590 889 1629 43
Fragments arranged by size:
1629 1425 889 590 480 275 269 193
188 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2960 3550 1537
Size: 275 1148 590 4090
Fragments arranged by size:
4090 1148 590 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 2966 4033 1353
Size: 882 731 1067 3423
Fragments arranged by size:
3423 1067 882 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4690 5064 5210 387
Size: 1064 138 3101 374 146 1280
Fragments arranged by size:
3101 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2282 2576 4166 4763 5550 5818
Size: 393 461 189 294 1590 597 787 268
Cuts at: 5818 1239
Size: 1524
Fragments arranged by size:
1590 1524 787 597 461 393 294 268
189
EcoNI CCTnn'n_nnAGG
Cuts at: 2166 2166
Size: 6103
EcoRI G'AATT_C
Cuts at: 2298 3412 3790 2298
Size: 1114 378 4611
Fragments arranged by size:
4611 1114 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2351 2351
Size: 6103
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 2057 5250 1678
Size: 379 3193 2531
Fragments arranged by size:
3193 2531 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2716 2748 2779
Size: 32 135 32 524 32 588 32 31
Cuts at: 2779 2811 4744 4776 4818 4850 1373
Size: 32 1933 32 42 32 2626
Fragments arranged by size:
2626 1933 588 524 135 42 32 32
32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 3447 3693 669
Size: 988 281 1509 246 3079
Fragments arranged by size:
3079 1509 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KpnI G_GTAC'C
Cuts at: 3428 3428
Size: 6103
MfeI C'AATT_G
Cuts at: 2605 3702 2605
Size: 1097 5006
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 2781 5217 5401 1305
Size: 780 696 2436 184 2007
Fragments arranged by size:
2436 2007 780 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 2774 10
Size: 54 1425 343 462 480 3339
Fragments arranged by size:
3339 1425 480 462 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 4061 4420 4579 518
Size: 648 1209 1686 359 159 2042
Fragments arranged by size:
2042 1686 1209 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2401 3247 3519 4196 5344 5589 1606
Size: 795 846 272 677 1148 245 2120
Fragments arranged by size:
2120 1148 846 795 677 272 245
NcoI C'CATG_G
Cuts at: 513 1065 2247 513
Size: 552 1182 4369
Fragments arranged by size:
4369 1182 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 2047 2146 3079 3461 5006 1132
Size: 97 818 99 933 382 1545 2229
Fragments arranged by size:
2229 1545 933 818 382 99 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 2142 3075 5002 2142
Size: 933 1927 3243
Fragments arranged by size:
3243 1927 933
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6103
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PmlI CAC'GTG
Cuts at: 2341 2341
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 5685 5717 4172
Size: 32 1481 32 4558
Fragments arranged by size:
4558 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PstI C_TGCA'G
Cuts at: 830 2088 2406 2676 2732 3243 3252 3455 830
Size: 1258 318 270 56 511 9 203 3478
Fragments arranged by size:
3478 1258 511 318 270 203 56 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
PvuII CAG'CTG
Cuts at: 2401 3247 2401
Size: 846 5257
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SapI GCTCTTCn'nnn_
Cuts at: 2235 2235
Size: 6103
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SfcI C'TryA_G
Cuts at: 826 1045 2084 2402 2480 2672 2728 3239 3248
Size: 219 1039 318 78 192 56 511 9
Cuts at: 3248 3451 4586 5267 5458 826
Size: 203 1135 681 191 1471
Fragments arranged by size:
1471 1135 1039 681 511 318 219 203
192 191 78 56 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 3430 1872
Size: 1558 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 3332 4207 5108 5370 820
Size: 197 926 69 1320 875 901 262 1553
Fragments arranged by size:
1553 1320 926 901 875 262 197 69
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 2047 3461 1132
Size: 915 1414 3774
Fragments arranged by size:
3774 1414 915
SspI AAT'ATT
Cuts at: 5 52 4027 5
Size: 47 3975 2081
Fragments arranged by size:
3975 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6103
StyI C'CwwG_G
Cuts at: 513 1065 2247 2637 2966 3067 3542 513
Size: 552 1182 390 329 101 475 3074
Fragments arranged by size:
3074 1182 552 475 390 329 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 3370 4130 4147 4300 4485 4930
Size: 1255 570 1220 760 17 153 185 445
Cuts at: 4930 325
Size: 1498
Fragments arranged by size:
1498 1255 1220 760 570 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 2754 4349
Size: 274 80 33 51 1443 168 609 1595
Cuts at: 4349 6061 96
Size: 1712 138
Fragments arranged by size:
1712 1595 1443 609 274 168 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 6063 6095 2184
Size: 32 3847 32 2192
Fragments arranged by size:
3847 2192 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6103
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3046 3046
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6103
XmaI C'CCGG_G
Cuts at: 1870 3428 1870
Size: 1558 4545
XmnI GAAnn'nnTTC
Cuts at: 2826 4232 2826
Size: 1406 4697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI