Restriction map for
pFN21AB5307
(Circular) (Six-base) MAPSORT of: pFN21AB5307.seq Check: 1289 from: 1 to: 5941
pFN21AB5307 5941 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2102 3192 3751 278
Size: 53 83 186 936 566 1090 559 2468
Fragments arranged by size:
2468 1090 936 566 559 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3192 3284 1937
Size: 1255 92 4594
Fragments arranged by size:
4594 1255 92
Acc65I G'GTAC_C
Cuts at: 2280 3262 2280
Size: 982 4959
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4004 5388 5656 1632
Size: 2372 1384 268 1917
Fragments arranged by size:
2372 1917 1384 268
AfeI AGC'GCT
Cuts at: 2141 2141
Size: 5941
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 2410 2479 3169 3684 5256 2410
Size: 69 690 515 1572 3095
Fragments arranged by size:
3095 1572 690 515 69
ApaI G_GGCC'C
Cuts at: 2092 2092
Size: 5941
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2133 3266 1870
Size: 73 93 97 1133 4545
Fragments arranged by size:
4545 1133 97 93 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2280 3074 3262 4717 618
Size: 325 217 627 493 794 188 1455 1842
Fragments arranged by size:
1842 1455 794 627 493 325 217 188
BanII G_rGCy'C
Cuts at: 1391 2092 3260 1391
Size: 701 1168 4072
Fragments arranged by size:
4072 1168 701
BbeI G_GCGC'C
Cuts at: 3078 3078
Size: 5941
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4970
BbvCI CC'TCA_GC
Cuts at: 2024 3012 2024
Size: 988 4953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 2931 1548
Size: 1383 4558
BfrBI ATG'CAT
Cuts at: 3067 3067
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4552 136
Size: 107 122 71 631 605 2880 1525
Fragments arranged by size:
2880 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2974 5936 2974
Size: 2962 2979
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2092 4002 5158 1790
Size: 302 1910 1156 2573
Fragments arranged by size:
2573 1910 1156 302
BmgBI CAC'GTC
Cuts at: 1318 3120 1318
Size: 1802 4139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2446 3073 3672
Size: 416 53 83 399 24 1029 627 599
Cuts at: 3672 4630 442
Size: 958 1753
Fragments arranged by size:
1753 1029 958 627 599 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2328 2218
Size: 110 5831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4601 1239
Size: 3362 2579
Bpu10I CC'TnA_GC
Cuts at: 2024 3012 2024
Size: 988 4953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2819 4066 4931 5229 2819
Size: 1247 865 298 3531
Fragments arranged by size:
3531 1247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3156 4604 882
Size: 617 1657 1448 2219
Fragments arranged by size:
2219 1657 1448 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 2377 2525 3732 493
Size: 711 918 255 148 1207 2702
Fragments arranged by size:
2702 1207 918 711 255 148
BsaBI GATnn'nnATC
Cuts at: 2925 5935 2925
Size: 3010 2931
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2099 2722 3075
Size: 53 83 186 936 482 84 623 353
Cuts at: 3075 3189 3748 4130 4765 275
Size: 114 559 382 635 1451
Fragments arranged by size:
1451 936 635 623 559 482 382 353
186 114 84 83 53
BsaWI w'CCGG_w
Cuts at: 3234 4374 4772 5046 5193 5604 3234
Size: 1140 398 274 147 411 3571
Fragments arranged by size:
3571 1140 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2067 2097 1178
Size: 30 165 30 664 30 5022
Fragments arranged by size:
5022 664 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 1186
Size: 855 5086
BseYI C'CCAG_C
Cuts at: 5144 5640 5144
Size: 496 5445
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 1163
Size: 573 621 4747
Fragments arranged by size:
4747 621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2278 2736 4002 4087 5158 2278
Size: 458 1266 85 1071 3061
Fragments arranged by size:
3061 1266 1071 458 85
BsmI GAATG_Cn'
Cuts at: 2845 3452 3545 2845
Size: 607 93 5241
Fragments arranged by size:
5241 607 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2681 2681
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 2278 2736 3260 4002 4087 5158
Size: 399 302 186 458 524 742 85 1071
Cuts at: 5158 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 524 458 399 302 186
85
BspEI T'CCGG_A
Cuts at: 3234 5604 3234
Size: 2370 3571
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2292 3204 3296 844
Size: 1448 912 92 3489
Fragments arranged by size:
3489 1448 912 92
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 2053 2094 2244 3828 1394
Size: 429 208 22 41 150 1584 3507
Fragments arranged by size:
3507 1584 429 208 150 41 22
BsrDI GCAATG_nn'
Cuts at: 65 1248 2752 4436 4610 65
Size: 1183 1504 1684 174 1396
Fragments arranged by size:
1684 1504 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4585 4772 1700
Size: 110 2775 187 2869
Fragments arranged by size:
2869 2775 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3169 794
Size: 2375 3566
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 2500 1184
Size: 1316 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3215 2011
Size: 1204 4737
BstYI r'GATC_y
Cuts at: 1972 2940 2974 3271 3638 4023 4040 5492 5578
Size: 968 34 297 367 385 17 1452 86
Cuts at: 5578 5590 5936 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 968 385 367 346 297 86
34 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2950 513
Size: 552 1885 3504
Fragments arranged by size:
3504 1885 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2893 487
Size: 683 56 1667 3535
Fragments arranged by size:
3535 1667 683 56
BtsI GCAGTG_nn'
Cuts at: 3457 4251 4271 3457
Size: 794 20 5127
Fragments arranged by size:
5127 794 20
ClaI AT'CG_AT
Cuts at: 1554 3213 1554
Size: 1659 4282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DraIII CAC_nnn'GTG
Cuts at: 2122 2122
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2175 2239
Size: 54 1425 50 275 18 273 72 64
Cuts at: 2239 2613 2770 3052 3217 3388 4277 5906 8
Size: 374 157 282 165 171 889 1629 43
Fragments arranged by size:
1629 1425 889 374 282 275 273 171
165 157 72 64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3871 1353
Size: 2518 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2198 2456 3194 4528 4902 5048
Size: 1064 138 609 258 738 1334 374 146
Cuts at: 5048 387
Size: 1280
Fragments arranged by size:
1334 1280 1064 738 609 374 258 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4004 4601 5388 5656 1239
Size: 393 2372 597 787 268 1524
Fragments arranged by size:
2372 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2069 2518 3028 3086 2069
Size: 449 510 58 4924
Fragments arranged by size:
4924 510 449 58
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2143 2633 3078 5088 1678
Size: 465 490 445 2010 2531
Fragments arranged by size:
2531 2010 490 465 445
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 2061 2093 4582
Size: 32 135 32 445 32 12 32 2489
Cuts at: 4582 4614 4656 4688 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2489 445 135 42 32 32 32
32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3229 748
Size: 2481 3460
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 3074 3074
Size: 5941
KpnI G_GTAC'C
Cuts at: 2284 3266 2284
Size: 982 4959
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 2772 3054 10
Size: 54 1425 343 783 157 282 2897
Fragments arranged by size:
2897 1425 783 343 282 157 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2379 2493 2739 2801 3213 3899
Size: 648 1059 154 114 246 62 412 686
Cuts at: 3899 4258 4417 518
Size: 359 159 2042
Fragments arranged by size:
2042 1059 686 648 412 359 246 159
154 114 62
MspA1I CmG'CkG
Cuts at: 1606 2192 2334 2464 2476 3160 3357 4034 5182
Size: 586 142 130 12 684 197 677 1148
Cuts at: 5182 5427 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 684 677 586 245 197 142
130 12
NarI GG'CG_CC
Cuts at: 3075 3075
Size: 5941
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5389
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NsiI A_TGCA'T
Cuts at: 3069 3069
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 2611 3067 3299 4844 1132
Size: 97 1382 456 232 1545 2229
Fragments arranged by size:
2229 1545 1382 456 232 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PmlI CAC'GTG
Cuts at: 2122 2377 2122
Size: 255 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2069 3028 3086 2069
Size: 959 58 4924
Fragments arranged by size:
4924 959 58
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2088 2088
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2289 3159 3165 3174 3293 830
Size: 1459 870 6 9 119 3478
Fragments arranged by size:
3478 1459 870 119 9 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2464 2476 3160 2464
Size: 12 684 5245
Fragments arranged by size:
5245 684 12
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 2069 2069
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3174 3293 3174
Size: 119 5822
ScaI AGT'ACT
Cuts at: 2456 4189 5901 2456
Size: 1733 1712 2496
Fragments arranged by size:
2496 1733 1712
SfcI C'TryA_G
Cuts at: 826 1045 2285 3155 3161 3170 3289 4424 5105
Size: 219 1240 870 6 9 119 1135 681
Cuts at: 5105 5296 826
Size: 191 1471
Fragments arranged by size:
1471 1240 1135 870 681 219 191 119
9 6
SfoI GGC'GCC
Cuts at: 3076 3076
Size: 5941
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2038 3268 1872
Size: 166 1230 4545
Fragments arranged by size:
4545 1230 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2834 4045 4946 5208 820
Size: 197 926 891 1211 901 262 1553
Fragments arranged by size:
1553 1211 926 901 891 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 3380 513
Size: 552 2315 3074
Fragments arranged by size:
3074 2315 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2695 3968 3985 4138 4323 4768 325
Size: 1255 1115 1273 17 153 185 445 1498
Fragments arranged by size:
1498 1273 1255 1115 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2454 2669 4187
Size: 274 80 33 51 1443 477 215 1518
Cuts at: 4187 5899 96
Size: 1712 138
Fragments arranged by size:
1712 1518 1443 477 274 215 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 5901 5933 2067
Size: 32 3802 32 2075
Fragments arranged by size:
3802 2075 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2654 2984 1316
Size: 1338 330 4273
Fragments arranged by size:
4273 1338 330
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2623 2623
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2036 3266 1870
Size: 166 1230 4545
Fragments arranged by size:
4545 1230 166
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2100 3190 3749 276
Size: 53 83 186 936 566 1090 559 2468
Fragments arranged by size:
2468 1090 936 566 559 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BsiWI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
PacI PfoI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI