Restriction map for
pFN21AB5306
(Circular) (Six-base) MAPSORT of: pFN21AB5306.seq Check: 9292 from: 1 to: 6643
pFN21AB5306 6643 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4453 278
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2666 3457 3986 1937
Size: 729 791 529 4594
Fragments arranged by size:
4594 791 729 529
Acc65I G'GTAC_C
Cuts at: 3964 3964
Size: 6643
AclI AA'CG_TT
Cuts at: 1563 4770 5143 1563
Size: 3207 373 3063
Fragments arranged by size:
3207 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3606 4706 6090 6358 1632
Size: 1974 1100 1384 268 1917
Fragments arranged by size:
1974 1917 1384 1100 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6446
AflIII A'CryG_T
Cuts at: 1203 5542 6519 1203
Size: 4339 977 1327
Fragments arranged by size:
4339 1327 977
AgeI A'CCGG_T
Cuts at: 5474 5474
Size: 6643
AhdI GACnn_n'nnGTC
Cuts at: 3610 5372 3610
Size: 1762 4881
AleI CACnn'nnGTG
Cuts at: 1166 2688 1166
Size: 1522 5121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6643
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3543 3575 3543
Size: 32 6611
AlwNI CAG_nnn'CTG
Cuts at: 4386 5958 4386
Size: 1572 5071
ApaI G_GGCC'C
Cuts at: 3924 3924
Size: 6643
ApaLI G'TGCA_C
Cuts at: 4700 5856 4700
Size: 1156 5487
ApoI r'AATT_y
Cuts at: 1493 3622 3776 3952 4178 4330 6439 1493
Size: 2129 154 176 226 152 2109 1697
Fragments arranged by size:
2129 2109 1697 226 176 154 152
AseI AT'TA_AT
Cuts at: 160 1032 2523 5197 160
Size: 872 1491 2674 1606
Fragments arranged by size:
2674 1606 1491 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
AvaI C'yCGr_G
Cuts at: 1870 1943 3408 3968 1870
Size: 73 1465 560 4545
Fragments arranged by size:
4545 1465 560 73
BamHI G'GATC_C
Cuts at: 3973 4340 3973
Size: 367 6276
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2939 3964 5419 618
Size: 325 217 627 252 900 1025 1455 1842
Fragments arranged by size:
1842 1455 1025 900 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 3895 3924 3962 1391
Size: 2504 29 38 4072
Fragments arranged by size:
4072 2504 38 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2355 2420 3549 928
Size: 971 456 65 1129 4022
Fragments arranged by size:
4022 1129 971 456 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2072 2106 4834 4868 1903
Size: 34 135 34 2728 34 3678
Fragments arranged by size:
3678 2728 135 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3041 4535 5745 3041
Size: 1494 1210 3939
Fragments arranged by size:
3939 1494 1210
BclI T'GATC_A
Cuts at: 1548 2098 1548
Size: 550 6093
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5254 136
Size: 107 122 71 631 605 3582 1525
Fragments arranged by size:
3582 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6638 6638
Size: 6643
BlpI GC'TnA_GC
Cuts at: 2814 4060 2814
Size: 1246 5397
Bme1580I G_kGCm'C
Cuts at: 1790 2905 3924 4704 5860 1790
Size: 1115 1019 780 1156 2573
Fragments arranged by size:
2573 1156 1115 1019 780
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6643
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2945 4374 5332
Size: 416 53 83 399 24 1528 1429 958
Cuts at: 5332 442
Size: 1753
Fragments arranged by size:
1753 1528 1429 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2694 3168 3821 5303 1239
Size: 1455 474 653 1482 2579
Fragments arranged by size:
2579 1482 1455 653 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3650 4768 5633 5931 3650
Size: 1118 865 298 4362
Fragments arranged by size:
4362 1118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2327 2507 5306 882
Size: 617 828 180 2799 2219
Fragments arranged by size:
2799 2219 828 617 180
BsaAI yAC'GTr
Cuts at: 493 1204 4434 493
Size: 711 3230 2702
Fragments arranged by size:
3230 2702 711
BsaBI GATnn'nnATC
Cuts at: 3509 3753 6637 3509
Size: 244 2884 3515
Fragments arranged by size:
3515 2884 244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2788 4450 4832 5467
Size: 53 83 186 936 1255 1662 382 635
Cuts at: 5467 275
Size: 1451
Fragments arranged by size:
1662 1451 1255 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5076 5474 5748 5895 6306 5076
Size: 398 274 147 411 5413
Fragments arranged by size:
5413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6418
Fragments arranged by size:
6418 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 3186 3885 1186
Size: 1183 817 699 3944
Fragments arranged by size:
3944 1183 817 699
BseYI C'CCAG_C
Cuts at: 5846 6342 5846
Size: 496 6147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3141 3387 3864 1163
Size: 573 1405 246 477 3942
Fragments arranged by size:
3942 1405 573 477 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4093 4854 5882 1540
Size: 275 186 2092 761 1028 2301
Fragments arranged by size:
2301 2092 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2592 3895 4704 4789 5860 2592
Size: 1303 809 85 1071 3375
Fragments arranged by size:
3375 1303 1071 809 85
BsmI GAATG_Cn'
Cuts at: 2926 3870 4154 4247 2926
Size: 944 284 93 5322
Fragments arranged by size:
5322 944 284 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2592 2905 3895 3924 3962 4704 4789
Size: 399 802 313 990 29 38 742 85
Cuts at: 4789 5860 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 990 802 742 399 313 85
38 29
BspEI T'CCGG_A
Cuts at: 6306 6306
Size: 6643
BspHI T'CATG_A
Cuts at: 2998 3231 4532 6262 2998
Size: 233 1301 1730 3379
Fragments arranged by size:
3379 1730 1301 233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2026 3375 3998 844
Size: 1182 1349 623 3489
Fragments arranged by size:
3489 1349 1182 623
BsrBI CCG'CTC
Cuts at: 1394 1823 4530 1394
Size: 429 2707 3507
Fragments arranged by size:
3507 2707 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2190 5138 5312 65
Size: 1183 942 2948 174 1396
Fragments arranged by size:
2948 1396 1183 942 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5287 5474 1700
Size: 110 3477 187 2869
Fragments arranged by size:
3477 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6643
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6643
BssSI C'ACGA_G
Cuts at: 4703 5715 4703
Size: 1012 5631
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6643
BstBI TT'CG_AA
Cuts at: 5447 5447
Size: 6643
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6643
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2690 2431
Size: 259 6384
BstYI r'GATC_y
Cuts at: 1972 3973 4340 4725 4742 6194 6280 6292 6638
Size: 2001 367 385 17 1452 86 12 346
Cuts at: 6638 1972
Size: 1977
Fragments arranged by size:
2001 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3458 3458
Size: 6643
Bsu36I CC'TnA_GG
Cuts at: 3916 3916
Size: 6643
BtgI C'CryG_G
Cuts at: 513 1065 2935 513
Size: 552 1870 4221
Fragments arranged by size:
4221 1870 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5904
Fragments arranged by size:
5904 683 56
BtsI GCAGTG_nn'
Cuts at: 4159 4953 4973 4159
Size: 794 20 5829
Fragments arranged by size:
5829 794 20
ClaI AT'CG_AT
Cuts at: 1554 2856 1554
Size: 1302 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6608
DraI TTT'AAA
Cuts at: 3947 3947
Size: 6643
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5650 809
Size: 507 4334 1802
Fragments arranged by size:
4334 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4090 4979 6608
Size: 54 1425 50 275 18 2260 889 1629
Cuts at: 6608 8
Size: 43
Fragments arranged by size:
2260 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4090 1537
Size: 275 2278 4090
Fragments arranged by size:
4090 2278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2833 3927 4573 1353
Size: 1480 1094 646 3423
Fragments arranged by size:
3423 1480 1094 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5230 5604 5750 387
Size: 1064 138 3641 374 146 1280
Fragments arranged by size:
3641 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3893 3893
Size: 6643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2694 3168 3606 3821 4706 5303 6090
Size: 393 1062 474 438 215 885 597 787
Cuts at: 6090 6358 1239
Size: 268 1524
Fragments arranged by size:
1524 1062 885 787 597 474 438 393
268 215
EcoO109I rG'GnC_Cy
Cuts at: 2015 3920 2015
Size: 1905 4738
EcoRI G'AATT_C
Cuts at: 3622 3952 4330 3622
Size: 330 378 5935
Fragments arranged by size:
5935 378 330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2423 2423
Size: 6643
FspI TGC'GCA
Cuts at: 5149 5149
Size: 6643
HaeII r_GCGC'y
Cuts at: 1678 5790 1678
Size: 4112 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2648 2680 2845
Size: 32 135 32 773 32 271 32 165
Cuts at: 2845 2877 3596 3628 5284 5316 5358 5390 1373
Size: 32 719 32 1656 32 42 32 2626
Fragments arranged by size:
2626 1656 773 719 271 165 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2719 3987 4233 669
Size: 988 281 781 1268 246 3079
Fragments arranged by size:
3079 1268 988 781 281 246
HindIII A'AGCT_T
Cuts at: 748 2341 3047 748
Size: 1593 706 4344
Fragments arranged by size:
4344 1593 706
HpaI GTT'AAC
Cuts at: 4233 4233
Size: 6643
KpnI G_GTAC'C
Cuts at: 3968 3968
Size: 6643
MfeI C'AATT_G
Cuts at: 4242 4242
Size: 6643
MluI A'CGCG_T
Cuts at: 6519 6519
Size: 6643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2551 5757 5941 1305
Size: 1246 3206 184 2007
Fragments arranged by size:
3206 2007 1246 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4821
Fragments arranged by size:
4821 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2688 4601 4960 5119 518
Size: 648 1522 1913 359 159 2042
Fragments arranged by size:
2042 1913 1522 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2672 3125 4059 4736 5884 6129 1606
Size: 1066 453 934 677 1148 245 2120
Fragments arranged by size:
2120 1148 1066 934 677 453 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6091
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6643
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6643
NotI GC'GGCC_GC
Cuts at: 4090 4090
Size: 6643
NspI r_CATG'y
Cuts at: 1132 1229 3856 4001 5546 1132
Size: 97 2627 145 1545 2229
Fragments arranged by size:
2627 2229 1545 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6643
PciI A'CATG_T
Cuts at: 5542 5542
Size: 6643
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6643
PfoI T'CCnGG_A
Cuts at: 3561 3561
Size: 6643
PmeI GTTT'AAAC
Cuts at: 3947 3947
Size: 6643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4712 4744 6225 6257 4712
Size: 32 1481 32 5098
Fragments arranged by size:
5098 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2015 2015
Size: 6643
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6643
PsiI TTA'TAA
Cuts at: 4213 4213
Size: 6643
PspOMI G'GGCC_C
Cuts at: 3920 3920
Size: 6643
PstI C_TGCA'G
Cuts at: 830 2023 3995 830
Size: 1193 1972 3478
Fragments arranged by size:
3478 1972 1193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6643
PvuII CAG'CTG
Cuts at: 2672 3125 2672
Size: 453 6190
SacI G_AGCT'C
Cuts at: 3895 3895
Size: 6643
SalI G'TCGA_C
Cuts at: 1936 3985 1936
Size: 2049 4594
SbfI CC_TGCA'GG
Cuts at: 3995 3995
Size: 6643
ScaI AGT'ACT
Cuts at: 4891 6603 4891
Size: 1712 4931
SfcI C'TryA_G
Cuts at: 826 1045 2019 2667 3991 5126 5807 5998 826
Size: 219 974 648 1324 1135 681 191 1471
Fragments arranged by size:
1471 1324 1135 974 681 648 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6643
SmaI CCC'GGG
Cuts at: 1872 3970 1872
Size: 2098 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3629 4747 5648 5910 820
Size: 197 926 1686 1118 901 262 1553
Fragments arranged by size:
1686 1553 1118 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4434 493
Size: 3941 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6643
SphI G_CATG'C
Cuts at: 1132 3856 4001 1132
Size: 2724 145 3774
Fragments arranged by size:
3774 2724 145
SspI AAT'ATT
Cuts at: 5 52 4567 5
Size: 47 4515 2081
Fragments arranged by size:
4515 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6643
StyI C'CwwG_G
Cuts at: 513 1065 2010 2307 2407 3205 4082 513
Size: 552 945 297 100 798 877 3074
Fragments arranged by size:
3074 945 877 798 552 297 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2048 2678 2696 2852 4670 4687 4840
Size: 1255 468 630 18 156 1818 17 153
Cuts at: 4840 5025 5470 325
Size: 185 445 1498
Fragments arranged by size:
1818 1498 1255 630 468 445 185 156
153 18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2509 3576 4889
Size: 274 80 33 51 1443 532 1067 1313
Cuts at: 4889 6601 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1313 1067 532 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6603 6635 6603
Size: 32 6611
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6643
XbaI T'CTAG_A
Cuts at: 3979 3979
Size: 6643
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6643
XmaI C'CCGG_G
Cuts at: 1870 3968 1870
Size: 2098 4545
XmnI GAAnn'nnTTC
Cuts at: 4772 4772
Size: 6643
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4451 276
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI