Restriction map for
pFN21AB5302
(Circular) (Six-base) MAPSORT of: pFN21AB5302.seq Check: 7226 from: 1 to: 5632
pFN21AB5302 5632 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2500 2975 1937
Size: 563 475 4594
Fragments arranged by size:
4594 563 475
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3695 5079 5347 1632
Size: 2063 1384 268 1917
Fragments arranged by size:
2063 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2312 820
Size: 197 1295 4140
Fragments arranged by size:
4140 1295 197
AflIII A'CryG_T
Cuts at: 1203 2899 4531 5508 1203
Size: 1696 1632 977 1327
Fragments arranged by size:
1696 1632 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 2711 1166
Size: 1545 4087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 2162 3375 4947 2162
Size: 1213 1572 2847
Fragments arranged by size:
2847 1572 1213
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2643 2957 1870
Size: 73 700 314 4545
Fragments arranged by size:
4545 700 314 73
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2754 2953 4408 618
Size: 325 217 627 967 199 1455 1842
Fragments arranged by size:
1842 1455 967 627 325 217 199
BanII G_rGCy'C
Cuts at: 1391 2725 2951 1391
Size: 1334 226 4072
Fragments arranged by size:
4072 1334 226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2129 2163 3823 3857 1903
Size: 34 192 34 1660 34 3678
Fragments arranged by size:
3678 1660 192 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2446 2490 5627 2446
Size: 44 3137 2451
Fragments arranged by size:
3137 2451 44
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 3693 4849 1790
Size: 1903 1156 2573
Fragments arranged by size:
2573 1903 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4292 1239
Size: 3053 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2516 4295 882
Size: 617 1017 1779 2219
Fragments arranged by size:
2219 1779 1017 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2763 4065 4463 4737 4884 5295 2763
Size: 1302 398 274 147 411 3100
Fragments arranged by size:
3100 1302 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2247 2277 1178
Size: 30 165 30 844 30 4533
Fragments arranged by size:
4533 844 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2631 2661 1186
Size: 1445 30 4157
Fragments arranged by size:
4157 1445 30
BseYI C'CCAG_C
Cuts at: 2875 4835 5331 2875
Size: 1960 496 3176
Fragments arranged by size:
3176 1960 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5059
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2551 2725 2791 3693 3778 4849 2551
Size: 174 66 902 85 1071 3334
Fragments arranged by size:
3334 1071 902 174 85 66
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2551 2725 2791 2951 3693 3778 4849
Size: 399 761 174 66 160 742 85 1071
Cuts at: 4849 1391
Size: 2174
Fragments arranged by size:
2174 1071 761 742 399 174 160 85
66
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 2191 2356 3521 5251 2191
Size: 165 1165 1730 2572
Fragments arranged by size:
2572 1730 1165 165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 844
Size: 2143 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 2813 1184
Size: 1629 4003
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2032
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2446 2490 2608 2962 3329 3714 3731 5183
Size: 474 44 118 354 367 385 17 1452
Cuts at: 5183 5269 5281 5627 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 474 385 367 354 346 118
86 44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2620 513
Size: 552 939 616 3525
Fragments arranged by size:
3525 939 616 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 2297 2469 3148 3942 3962 2297
Size: 172 679 794 20 3967
Fragments arranged by size:
3967 794 679 172 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3079 3968 5597
Size: 54 1425 50 275 18 1249 889 1629
Cuts at: 5597 8
Size: 43
Fragments arranged by size:
1629 1425 1249 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 1353
Size: 2209 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2723 2723
Size: 5632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3695 4292 5079 5347 1239
Size: 393 2063 597 787 268 1524
Fragments arranged by size:
2063 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2886 2886
Size: 5632
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 4779 1678
Size: 3101 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2482 2514 2674 2706 4273
Size: 32 135 32 910 32 160 32 1567
Cuts at: 4273 4305 4347 4379 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1567 910 160 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 2976 3222 669
Size: 988 281 328 710 246 3079
Fragments arranged by size:
3079 988 710 328 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2157 4746 4930 1305
Size: 852 2589 184 2007
Fragments arranged by size:
2589 2007 852 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3810
Fragments arranged by size:
3810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2697 2711 3590 3949 4108 518
Size: 648 1531 14 879 359 159 2042
Fragments arranged by size:
2042 1531 879 648 359 159 14
MspA1I CmG'CkG
Cuts at: 1606 2053 2071 3048 3725 4873 5118 1606
Size: 447 18 977 677 1148 245 2120
Fragments arranged by size:
2120 1148 977 677 447 245 18
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4141
Fragments arranged by size:
4141 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2903 2990 4535 1132
Size: 97 1674 87 1545 2229
Fragments arranged by size:
2229 1674 1545 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 2899 4531 2899
Size: 1632 4000
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2003 2035 3701 3733 5214 5246 2003
Size: 32 1666 32 1481 32 2389
Fragments arranged by size:
2389 1666 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspXI vC'TCGA_Gb
Cuts at: 2643 2643
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2984 830
Size: 2154 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2053 2071 2053
Size: 18 5614
SacI G_AGCT'C
Cuts at: 2725 2725
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2523 2980 4115 4796 4987 826
Size: 219 1478 457 1135 681 191 1471
Fragments arranged by size:
1478 1471 1135 681 457 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 2643 3736 4637 4899 820
Size: 197 926 369 331 1093 901 262 1553
Fragments arranged by size:
1553 1093 926 901 369 331 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 2438 3556 5
Size: 47 2386 1118 2081
Fragments arranged by size:
2386 2081 1118 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 2475 3071 513
Size: 552 939 21 450 596 3074
Fragments arranged by size:
3074 939 596 552 450 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3878 5590 96
Size: 274 80 33 51 1443 1901 1712 138
Fragments arranged by size:
1901 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2717 2968 2717
Size: 251 5381
XhoI C'TCGA_G
Cuts at: 1943 2643 1943
Size: 700 4932
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 2018 3761 2018
Size: 1743 3889
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI