Restriction map for
pFN21AB5299
(Circular) (Six-base) MAPSORT of: pFN21AB5299.seq Check: 274 from: 1 to: 6061
pFN21AB5299 6061 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2172 2172
Size: 6061
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3019 3404 1937
Size: 1082 385 4594
Fragments arranged by size:
4594 1082 385
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 2387 3000 4124 5508 5776 1632
Size: 707 48 613 1124 1384 268 1917
Fragments arranged by size:
1917 1384 1124 707 613 268 48
AfeI AGC'GCT
Cuts at: 2124 2124
Size: 6061
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 2794 4960 5937 1203
Size: 1591 2166 977 1327
Fragments arranged by size:
2166 1591 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 2795 1166
Size: 1629 4432
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 3328 3804 5376 3328
Size: 476 1572 4013
Fragments arranged by size:
4013 1572 476
ApaLI G'TGCA_C
Cuts at: 2510 4118 5274 2510
Size: 1608 1156 3297
Fragments arranged by size:
3297 1608 1156
ApoI r'AATT_y
Cuts at: 1493 2046 3370 3596 3748 5857 1493
Size: 553 1324 226 152 2109 1697
Fragments arranged by size:
2109 1697 1324 553 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2591 3386 1870
Size: 73 175 473 795 4545
Fragments arranged by size:
4545 795 473 175 73
BamHI G'GATC_C
Cuts at: 3118 3391 3758 3118
Size: 273 367 5421
Fragments arranged by size:
5421 367 273
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3382 4837 618
Size: 325 217 627 1595 1455 1842
Fragments arranged by size:
1842 1595 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2072 2659 3196 3380 1391
Size: 681 587 537 184 4072
Fragments arranged by size:
4072 681 587 537 184
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2145 2940 928
Size: 971 246 795 4049
Fragments arranged by size:
4049 971 795 246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2134 2168 4252 4286 1903
Size: 34 197 34 2084 34 3678
Fragments arranged by size:
3678 2084 197 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2514 4122 5278 1790
Size: 724 1608 1156 2573
Fragments arranged by size:
2573 1608 1156 724
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2066 2200 3792
Size: 416 53 83 399 24 649 134 1592
Cuts at: 3792 4750 442
Size: 958 1753
Fragments arranged by size:
1753 1592 958 649 416 399 134 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2397 2158
Size: 239 5822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2046 2206 2533 3326 4721 1239
Size: 807 160 327 793 1395 2579
Fragments arranged by size:
2579 1395 807 793 327 160
Bpu10I CC'TnA_GC
Cuts at: 2126 2658 2126
Size: 532 5529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2198 4186 5051 5349 2198
Size: 1988 865 298 2910
Fragments arranged by size:
2910 1988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2968 4724 882
Size: 617 1469 1756 2219
Fragments arranged by size:
2219 1756 1469 617
BsaAI yAC'GTr
Cuts at: 493 1204 3852 493
Size: 711 2648 2702
Fragments arranged by size:
2702 2648 711
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 3868 4250 4885
Size: 53 83 186 936 563 1772 382 635
Cuts at: 4885 275
Size: 1451
Fragments arranged by size:
1772 1451 936 635 563 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2607 3108 4494 4892 5166 5313 5724 2607
Size: 501 1386 398 274 147 411 2944
Fragments arranged by size:
2944 1386 501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5836
Fragments arranged by size:
5836 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2313 1186
Size: 1127 4934
BseYI C'CCAG_C
Cuts at: 2740 2813 5264 5760 2740
Size: 73 2451 496 3041
Fragments arranged by size:
3041 2451 496 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2343 2514 2659 2792 2988 3312 4122 4207 5278
Size: 171 145 133 196 324 810 85 1071
Cuts at: 5278 2343
Size: 3126
Fragments arranged by size:
3126 1071 810 324 196 171 145 133
85
BsmI GAATG_Cn'
Cuts at: 3362 3572 3665 3362
Size: 210 93 5758
Fragments arranged by size:
5758 210 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2343 2514 2659 2792 2988 3196
Size: 399 282 271 171 145 133 196 208
Cuts at: 3196 3312 3380 4122 4207 5278 1391
Size: 116 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 282 271 208 196
171 145 133 116 85 68
BspEI T'CCGG_A
Cuts at: 3108 5724 3108
Size: 2616 3445
BspHI T'CATG_A
Cuts at: 3282 3950 5680 3282
Size: 668 1730 3663
Fragments arranged by size:
3663 1730 668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 3416 844
Size: 1328 1244 3489
Fragments arranged by size:
3489 1328 1244
BsrBI CCG'CTC
Cuts at: 1394 1823 2547 3948 1394
Size: 429 724 1401 3507
Fragments arranged by size:
3507 1401 724 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2574 3060 4705 4892 1700
Size: 110 764 486 1645 187 2869
Fragments arranged by size:
2869 1645 764 486 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2992 794
Size: 2198 3863
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 2553 1184
Size: 1369 4692
BstXI CCAn_nnnn'nTGG
Cuts at: 2184 3130 2184
Size: 946 5115
BstYI r'GATC_y
Cuts at: 1972 3118 3391 3758 4143 4160 5612 5698 5710
Size: 1146 273 367 385 17 1452 86 12
Cuts at: 5710 6056 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1146 385 367 346 273 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2499 2721 2856 513
Size: 552 1434 222 135 3718
Fragments arranged by size:
3718 1434 552 222 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 2734 3577 4371 4391 2734
Size: 843 794 20 4404
Fragments arranged by size:
4404 843 794 20
ClaI AT'CG_AT
Cuts at: 1554 2317 1554
Size: 763 5298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DraIII CAC_nnn'GTG
Cuts at: 2857 2857
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3289 3508 4397
Size: 54 1425 50 275 18 1459 219 889
Cuts at: 4397 6026 8
Size: 1629 43
Fragments arranged by size:
1629 1459 1425 889 275 219 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2976 3991 1353
Size: 1623 1015 3423
Fragments arranged by size:
3423 1623 1015
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2138 2621 4648 5022 5168 387
Size: 1064 138 549 483 2027 374 146 1280
Fragments arranged by size:
2027 1280 1064 549 483 374 146 138
EcoICRI GAG'CTC
Cuts at: 2657 2657
Size: 6061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2206 2339 2387 2533 3000 3326
Size: 393 414 160 133 48 146 467 326
Cuts at: 3326 4124 4721 5508 5776 1239
Size: 798 597 787 268 1524
Fragments arranged by size:
1524 798 787 597 467 414 393 326
268 160 146 133 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2328 2328
Size: 6061
EcoO109I rG'GnC_Cy
Cuts at: 2484 2663 2484
Size: 179 5882
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 2160 4567 2160
Size: 2407 3654
HaeII r_GCGC'y
Cuts at: 1678 2126 5208 1678
Size: 448 3082 2531
Fragments arranged by size:
3082 2531 448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2005 2037 3080 3103 3112
Size: 32 135 32 433 32 1043 23 9
Cuts at: 3112 3135 3170 3202 4702 4734 4776 4808 1373
Size: 23 35 32 1500 32 42 32 2626
Fragments arranged by size:
2626 1500 1043 433 135 42 35 32
32 32 32 32 32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 2247 3660 2247
Size: 1413 4648
MluI A'CGCG_T
Cuts at: 2794 5937 2794
Size: 3143 2918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4239
Fragments arranged by size:
4239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2331 2795 4019 4378 4537 518
Size: 648 1165 464 1224 359 159 2042
Fragments arranged by size:
2042 1224 1165 648 464 359 159
MspA1I CmG'CkG
Cuts at: 1606 2599 2806 3477 4154 5302 5547 1606
Size: 993 207 671 677 1148 245 2120
Fragments arranged by size:
2120 1148 993 677 671 245 207
NcoI C'CATG_G
Cuts at: 513 1065 2721 513
Size: 552 1656 3853
Fragments arranged by size:
3853 1656 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 3419 4964 1132
Size: 97 2190 1545 2229
Fragments arranged by size:
2229 2190 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2959 3328 1108
Size: 1851 369 3841
Fragments arranged by size:
3841 1851 369
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2484 2663 2484
Size: 179 5882
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspXI vC'TCGA_Gb
Cuts at: 2118 2118
Size: 6061
PstI C_TGCA'G
Cuts at: 830 3413 830
Size: 2583 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2806 2806
Size: 6061
SacI G_AGCT'C
Cuts at: 2659 2659
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SanDI GG'GwC_CC
Cuts at: 2663 2663
Size: 6061
SapI GCTCTTCn'nnn_
Cuts at: 2976 2976
Size: 6061
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 3016 3409 4544 5225 5416 826
Size: 219 1971 393 1135 681 191 1471
Fragments arranged by size:
1971 1471 1135 681 393 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2118 2213 4165 5066 5328 820
Size: 197 926 175 95 1952 901 262 1553
Fragments arranged by size:
1952 1553 926 901 262 197 175 95
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3105 3985 5
Size: 47 3053 880 2081
Fragments arranged by size:
3053 2081 880 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2721 3500 513
Size: 552 1656 779 3074
Fragments arranged by size:
3074 1656 779 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4088 4105 4258 4443 4888 325
Size: 1255 2508 17 153 185 445 1498
Fragments arranged by size:
2508 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4307 6019 96
Size: 274 80 33 51 1443 2330 1712 138
Fragments arranged by size:
2330 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2388 2420 2681 2713 2784 2816 3302 3334 6021
Size: 32 261 32 71 32 486 32 2687
Cuts at: 6021 6053 2388
Size: 32 2396
Fragments arranged by size:
2687 2396 486 261 71 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2497
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 2118 1943
Size: 175 5886
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 2352 4190 2352
Size: 1838 4223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI