Restriction map for
pFN21AB5297
(Circular) (Six-base) MAPSORT of: pFN21AB5297.seq Check: 5432 from: 1 to: 5605
pFN21AB5297 5605 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 2948 1937
Size: 343 668 4594
Fragments arranged by size:
4594 668 343
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3668 5052 5320 1632
Size: 2036 1384 268 1917
Fragments arranged by size:
2036 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 2530 2645 4504 5481 1203
Size: 1327 115 1859 977 1327
Fragments arranged by size:
1859 1327 1327 977 115
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 2135 3348 4920 2135
Size: 1213 1572 2820
Fragments arranged by size:
2820 1572 1213
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2009 2168 2914 3140 3292 5401 1493
Size: 516 159 746 226 152 2109 1697
Fragments arranged by size:
2109 1697 746 516 226 159 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 1870
Size: 73 987 4545
Fragments arranged by size:
4545 987 73
BamHI G'GATC_C
Cuts at: 2817 2935 3302 2817
Size: 118 367 5120
Fragments arranged by size:
5120 367 118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 2926 4381 618
Size: 325 217 627 468 671 1455 1842
Fragments arranged by size:
1842 1455 671 627 468 325 217
BanII G_rGCy'C
Cuts at: 1391 2924 1391
Size: 1533 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BfrBI ATG'CAT
Cuts at: 2746 2746
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 3666 4822 1790
Size: 1876 1156 2573
Fragments arranged by size:
2573 1876 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2261 3336 4294
Size: 416 53 83 399 24 844 1075 958
Cuts at: 4294 442
Size: 1753
Fragments arranged by size:
1753 1075 958 844 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2652 4265 1239
Size: 1004 409 1613 2579
Fragments arranged by size:
2579 1613 1004 409
Bpu10I CC'TnA_GC
Cuts at: 2826 2826
Size: 5605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4268 882
Size: 617 2769 2219
Fragments arranged by size:
2769 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3396 493
Size: 711 2192 2702
Fragments arranged by size:
2702 2192 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2107 1186
Size: 921 4684
BseYI C'CCAG_C
Cuts at: 2135 4808 5304 2135
Size: 2673 496 2436
Fragments arranged by size:
2673 2436 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5032
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3666 3751 4822 3666
Size: 85 1071 4449
Fragments arranged by size:
4449 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2924 3666 3751 4822 1391
Size: 399 1134 742 85 1071 2174
Fragments arranged by size:
2174 1134 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2960 844
Size: 2116 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2484 4100 4274 65
Size: 1183 1236 1616 174 1396
Fragments arranged by size:
1616 1396 1236 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2108 4249 4436 1700
Size: 110 298 2141 187 2869
Fragments arranged by size:
2869 2141 298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2404 2817 2935 3302 3687 3704 5156 5242
Size: 432 413 118 367 385 17 1452 86
Cuts at: 5242 5254 5600 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 432 413 385 367 346 118
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2721 2721
Size: 5605
BtgI C'CryG_G
Cuts at: 513 1065 2494 513
Size: 552 1429 3624
Fragments arranged by size:
3624 1429 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 3121 3915 3935 3121
Size: 794 20 4791
Fragments arranged by size:
4791 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2847 2909 2847
Size: 62 5543
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2497 3052 3941
Size: 54 1425 50 275 18 667 555 889
Cuts at: 3941 5570 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 667 555 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3535 1353
Size: 2182 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2652 3668 4265 5052 5320 1239
Size: 393 611 409 1016 597 787 268 1524
Fragments arranged by size:
1524 1016 787 611 597 409 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2705 2705
Size: 5605
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4246 4278 4320 4352 1373
Size: 32 135 32 2674 32 42 32 2626
Fragments arranged by size:
2674 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2075 748
Size: 1327 4278
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2563 4719 4903 1305
Size: 1258 2156 184 2007
Fragments arranged by size:
2156 2007 1258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2499 10
Size: 54 1425 343 667 3116
Fragments arranged by size:
3116 1425 667 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2650 3563 3922 4081 518
Size: 648 1484 913 359 159 2042
Fragments arranged by size:
2042 1484 913 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2234 2525 3021 3698 4846 5091 1606
Size: 628 291 496 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 628 496 291 245
NcoI C'CATG_G
Cuts at: 513 1065 2494 513
Size: 552 1429 3624
Fragments arranged by size:
3624 1429 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NsiI A_TGCA'T
Cuts at: 2748 2748
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2534 2649 2963 4508 1132
Size: 97 1305 115 314 1545 2229
Fragments arranged by size:
2229 1545 1305 314 115 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 2530 2645 4504 2530
Size: 115 1859 3631
Fragments arranged by size:
3631 1859 115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2705 2705
Size: 5605
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2288 2957 830
Size: 1458 669 3478
Fragments arranged by size:
3478 1458 669
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2234 2525 2234
Size: 291 5314
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SanDI GG'GwC_CC
Cuts at: 2705 2705
Size: 5605
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2284 2790 2953 4088 4769 4960 826
Size: 219 1239 506 163 1135 681 191 1471
Fragments arranged by size:
1471 1239 1135 681 506 219 191 163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 2108 1700
Size: 408 5197
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2259 2494 3044 513
Size: 552 1194 235 550 3074
Fragments arranged by size:
3074 1194 552 550 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2243 2527 3632 3649 3802 3987 4432
Size: 1255 663 284 1105 17 153 185 445
Cuts at: 4432 325
Size: 1498
Fragments arranged by size:
1498 1255 1105 663 445 284 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2437 2693 3851
Size: 274 80 33 51 1443 460 256 1158
Cuts at: 3851 5563 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1158 460 274 256 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI