Restriction map for
pFN21AB5294
(Circular) (Six-base) MAPSORT of: pFN21AB5294.seq Check: 5148 from: 1 to: 5734
pFN21AB5294 5734 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2482 2482
Size: 5734
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 2450 3861 4234 1563
Size: 887 1411 373 3063
Fragments arranged by size:
3063 1411 887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 3797 5181 5449 1632
Size: 785 1380 1384 268 1917
Fragments arranged by size:
1917 1384 1380 785 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 2886 4633 5610 1203
Size: 1683 1747 977 1327
Fragments arranged by size:
1747 1683 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2450 2482 2450
Size: 32 5702
AlwNI CAG_nnn'CTG
Cuts at: 3477 5049 3477
Size: 1572 4162
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2389 3059 1870
Size: 73 446 670 4545
Fragments arranged by size:
4545 670 446 73
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3055 4510 618
Size: 325 217 627 1268 1455 1842
Fragments arranged by size:
1842 1455 1268 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2718 3053 1391
Size: 1327 335 4072
Fragments arranged by size:
4072 1327 335
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2710 2744 3925 3959 1903
Size: 34 50 34 689 34 1181 34 3678
Fragments arranged by size:
3678 1181 689 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2150 2612
Size: 107 122 71 631 605 337 141 462
Cuts at: 2612 4345 136
Size: 1733 1525
Fragments arranged by size:
1733 1525 631 605 462 337 141 122
107 71
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2519 3795 4951 1790
Size: 729 1276 1156 2573
Fragments arranged by size:
2573 1276 1156 729
BmgBI CAC'GTC
Cuts at: 1318 2889 1318
Size: 1571 4163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2008 2498 3024 4394 1239
Size: 769 490 526 1370 2579
Fragments arranged by size:
2579 1370 769 526 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3859 4724 5022 3859
Size: 865 298 4571
Fragments arranged by size:
4571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2474 2852 4397 882
Size: 617 975 378 1545 2219
Fragments arranged by size:
2219 1545 975 617 378
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 3026 5728 3026
Size: 2702 3032
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2089 3541 3923 4558
Size: 53 83 186 936 556 1452 382 635
Cuts at: 4558 275
Size: 1451
Fragments arranged by size:
1452 1451 936 635 556 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 1178
Size: 30 165 30 1047 30 4432
Fragments arranged by size:
4432 1047 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 1186
Size: 1618 4116
BseYI C'CCAG_C
Cuts at: 2593 4937 5433 2593
Size: 2344 496 2894
Fragments arranged by size:
2894 2344 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5161
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3945 4973 1540
Size: 275 186 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3795 3880 4951 3795
Size: 85 1071 4578
Fragments arranged by size:
4578 1071 85
BsmI GAATG_Cn'
Cuts at: 2230 2760 2985 3245 3338 2230
Size: 530 225 260 93 4626
Fragments arranged by size:
4626 530 260 225 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2519 2718 3053 3795 3880 4951 1391
Size: 399 729 199 335 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 729 399 335 199 85
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 2988 3623 5353 2988
Size: 635 1730 3369
Fragments arranged by size:
3369 1730 635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2482 3089 844
Size: 1638 607 3489
Fragments arranged by size:
3489 1638 607
BsrBI CCG'CTC
Cuts at: 1394 1823 2388 2619 3621 1394
Size: 429 565 231 1002 3507
Fragments arranged by size:
3507 1002 565 429 231
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2158 2438 4378 4565 1700
Size: 110 348 280 1940 187 2869
Fragments arranged by size:
2869 1940 348 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5734
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstXI CCAn_nnnn'nTGG
Cuts at: 3002 3002
Size: 5734
BstYI r'GATC_y
Cuts at: 1972 3064 3431 3816 3833 5285 5371 5383 5729
Size: 1092 367 385 17 1452 86 12 346
Cuts at: 5729 1972
Size: 1977
Fragments arranged by size:
1977 1452 1092 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2799 2799
Size: 5734
BtgI C'CryG_G
Cuts at: 513 1065 2955 513
Size: 552 1890 3292
Fragments arranged by size:
3292 1890 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2713 2755 487
Size: 683 56 1487 42 3466
Fragments arranged by size:
3466 1487 683 56 42
BtsI GCAGTG_nn'
Cuts at: 2878 3250 4044 4064 2878
Size: 372 794 20 4548
Fragments arranged by size:
4548 794 372 20
ClaI AT'CG_AT
Cuts at: 1554 2901 1554
Size: 1347 4387
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2128 2217
Size: 54 1425 50 275 18 180 118 89
Cuts at: 2217 2604 3181 4070 5699 8
Size: 387 577 889 1629 43
Fragments arranged by size:
1629 1425 889 577 387 275 180 118
89 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 3664 1353
Size: 999 1312 3423
Fragments arranged by size:
3423 1312 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2168 4321 4695 4841 387
Size: 1064 138 579 2153 374 146 1280
Fragments arranged by size:
2153 1280 1064 579 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 2417 2498 3024 3797 4394 5181
Size: 393 376 409 81 526 773 597 787
Cuts at: 5181 5449 1239
Size: 268 1524
Fragments arranged by size:
1524 787 773 597 526 409 393 376
268 81
EcoNI CCTnn'n_nnAGG
Cuts at: 2409 2409
Size: 5734
EcoO109I rG'GnC_Cy
Cuts at: 2401 2422 2401
Size: 21 5713
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2261 2525 2848 2261
Size: 264 323 5147
Fragments arranged by size:
5147 323 264
FspI TGC'GCA
Cuts at: 2810 4240 2810
Size: 1430 4304
HaeII r_GCGC'y
Cuts at: 1678 4881 1678
Size: 3203 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2116 2148 4375 4407 4449
Size: 32 135 32 544 32 2227 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 2227 544 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2431 3078 3324 669
Size: 988 281 493 647 246 3079
Fragments arranged by size:
3079 988 647 493 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 2431 3324 2431
Size: 893 4841
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2114 3039 4848 5032 1305
Size: 809 925 1809 184 2007
Fragments arranged by size:
2007 1809 925 809 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 2219 2606 10
Size: 54 1425 343 180 207 387 3138
Fragments arranged by size:
3138 1425 387 343 207 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3692 4051 4210 518
Size: 648 2526 359 159 2042
Fragments arranged by size:
2526 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3150 3827 4975 5220 1606
Size: 1544 677 1148 245 2120
Fragments arranged by size:
2120 1544 1148 677 245
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 5734
NcoI C'CATG_G
Cuts at: 513 1065 2955 513
Size: 552 1890 3292
Fragments arranged by size:
3292 1890 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 2419 2593 3092 4637 1132
Size: 97 1190 174 499 1545 2229
Fragments arranged by size:
2229 1545 1190 499 174 97
PasI CC'CwG_GG
Cuts at: 1348 2689 1348
Size: 1341 4393
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2956 1108
Size: 1848 3886
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2450 2482 3803 3835 5316 5348 2450
Size: 32 1321 32 1481 32 2836
Fragments arranged by size:
2836 1481 1321 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2401 2401
Size: 5734
PshAI GACnn'nnGTC
Cuts at: 1613 2032 1613
Size: 419 5315
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PspXI vC'TCGA_Gb
Cuts at: 2389 2389
Size: 5734
PstI C_TGCA'G
Cuts at: 830 2351 2870 3086 830
Size: 1521 519 216 3478
Fragments arranged by size:
3478 1521 519 216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 2831 3982 5694 2831
Size: 1151 1712 2871
Fragments arranged by size:
2871 1712 1151
SfcI C'TryA_G
Cuts at: 826 1045 2347 2866 3082 4217 4898 5089 826
Size: 219 1302 519 216 1135 681 191 1471
Fragments arranged by size:
1471 1302 1135 681 519 219 216 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2612 2612
Size: 5734
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2389 3838 4739 5001 820
Size: 197 926 446 1449 901 262 1553
Fragments arranged by size:
1553 1449 926 901 446 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 2593 3092 1132
Size: 1461 499 3774
Fragments arranged by size:
3774 1461 499
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2955 3173 513
Size: 552 1890 218 3074
Fragments arranged by size:
3074 1890 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2334 3761 3778 3931 4116 4561 325
Size: 1255 754 1427 17 153 185 445 1498
Fragments arranged by size:
1498 1427 1255 754 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 3980 5692
Size: 274 80 33 51 1443 852 1151 1712
Cuts at: 5692 96
Size: 138
Fragments arranged by size:
1712 1443 1151 852 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5694 5726 5694
Size: 32 5702
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 2389 1943
Size: 446 5288
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI XcmI