Restriction map for
pFN21AB5292
(Circular) (Six-base) MAPSORT of: pFN21AB5292.seq Check: 1653 from: 1 to: 5941
pFN21AB5292 5941 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3168 3751 278
Size: 53 83 186 936 1632 583 2468
Fragments arranged by size:
2468 1632 936 583 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 2408 2451 3262 2408
Size: 43 811 5087
Fragments arranged by size:
5087 811 43
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3044 4004 5388 5656 1632
Size: 1412 960 1384 268 1917
Fragments arranged by size:
1917 1412 1384 960 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 2470 4670 2470
Size: 2200 3741
AleI CACnn'nnGTG
Cuts at: 1166 2396 1166
Size: 1230 4711
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2675 2707 2675
Size: 32 5909
AlwNI CAG_nnn'CTG
Cuts at: 2504 2671 3684 5256 2504
Size: 167 1013 1572 3189
Fragments arranged by size:
3189 1572 1013 167
ApaI G_GGCC'C
Cuts at: 2202 2915 2202
Size: 713 5228
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2207 2434 3266 1870
Size: 73 264 227 832 4545
Fragments arranged by size:
4545 832 264 227 73
BamHI G'GATC_C
Cuts at: 2073 2358 3153 3271 3638 2073
Size: 285 795 118 367 4376
Fragments arranged by size:
4376 795 367 285 118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 2408 2451 2476 3262
Size: 325 217 627 392 229 43 25 786
Cuts at: 3262 4717 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 786 627 392 325 229 217
43 25
BanII G_rGCy'C
Cuts at: 1391 2129 2202 2303 2915 3260 1391
Size: 738 73 101 612 345 4072
Fragments arranged by size:
4072 738 612 345 101 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2281 2638 3098 3160 928
Size: 971 382 357 460 62 3709
Fragments arranged by size:
3709 971 460 382 357 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4132 4166 1903
Size: 34 50 34 2111 34 3678
Fragments arranged by size:
3678 2111 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2951 3108 4552
Size: 107 122 71 631 605 1279 157 1444
Cuts at: 4552 136
Size: 1525
Fragments arranged by size:
1525 1444 1279 631 605 157 122 107
71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2202 2481 2915 4002 5158 1790
Size: 412 279 434 1087 1156 2573
Fragments arranged by size:
2573 1156 1087 434 412 279
BmgBI CAC'GTC
Cuts at: 1318 2094 1318
Size: 776 5165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2230 2266 3672
Size: 416 53 83 399 24 813 36 1406
Cuts at: 3672 4630 442
Size: 958 1753
Fragments arranged by size:
1753 1406 958 813 416 399 83 53
36 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 4601 1239
Size: 760 2602 2579
Fragments arranged by size:
2602 2579 760
Bpu10I CC'TnA_GC
Cuts at: 2456 2702 3104 2456
Size: 246 402 5293
Fragments arranged by size:
5293 402 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 3056 4066 4931 5229 2201
Size: 855 1010 865 298 2913
Fragments arranged by size:
2913 1010 865 855 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2398 3732 493
Size: 711 1194 1334 2702
Fragments arranged by size:
2702 1334 1194 711
BsaBI GATnn'nnATC
Cuts at: 2255 5935 2255
Size: 3680 2261
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2264 3165 3748
Size: 53 83 186 936 699 32 901 583
Cuts at: 3748 4130 4765 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 901 699 635 583 382 186
83 53 32
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 2675 2705 1178
Size: 30 165 30 882 30 360 30 4414
Fragments arranged by size:
4414 882 360 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2293 1186
Size: 1107 4834
BseYI C'CCAG_C
Cuts at: 2743 3028 3137 5144 5640 2743
Size: 285 109 2007 496 3044
Fragments arranged by size:
3044 2007 496 285 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2034 2066 1163
Size: 573 298 32 5038
Fragments arranged by size:
5038 573 298 32
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3391 4152 5180 1540
Size: 275 186 1177 213 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 213 186
BsiHKAI G_wGCw'C
Cuts at: 2217 4002 4087 5158 2217
Size: 1785 85 1071 3000
Fragments arranged by size:
3000 1785 1071 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 2164 2164
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2202 2217 2303 2481 2915 3260
Size: 399 339 73 15 86 178 434 345
Cuts at: 3260 4002 4087 5158 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 434 399 345 339 178
86 85 73 15
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2524 2806 3296 844
Size: 1680 282 490 3489
Fragments arranged by size:
3489 1680 490 282
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 3108 3828 1394
Size: 429 206 1079 720 3507
Fragments arranged by size:
3507 1079 720 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4585 4772 1700
Size: 110 2775 187 2869
Fragments arranged by size:
2869 2775 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2073 2358 3153 3271 3638 4023 4040 5492
Size: 101 285 795 118 367 385 17 1452
Cuts at: 5492 5578 5590 5936 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 795 385 367 346 285 118
101 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5389
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2332 2560 487
Size: 683 56 1106 228 3868
Fragments arranged by size:
3868 1106 683 228 56
BtsI GCAGTG_nn'
Cuts at: 2329 3457 4251 4271 2329
Size: 1128 794 20 3999
Fragments arranged by size:
3999 1128 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2841 3175 3388
Size: 54 1425 50 275 18 1011 334 213
Cuts at: 3388 4277 5906 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1011 889 334 275 213 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 3388 1537
Size: 275 1363 213 4090
Fragments arranged by size:
4090 1363 275 213
EarI CTCTTCn'nnn_
Cuts at: 1353 3871 1353
Size: 2518 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 4528 4902 5048 387
Size: 1064 138 501 2438 374 146 1280
Fragments arranged by size:
2438 1280 1064 501 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 3044 4004 4601 5388 5656 1239
Size: 393 367 1045 960 597 787 268 1524
Fragments arranged by size:
1524 1045 960 787 597 393 367 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2459 2828 2861 2459
Size: 369 33 5539
Fragments arranged by size:
5539 369 33
EcoO109I rG'GnC_Cy
Cuts at: 2137 2153 2198 2317 2710 2137
Size: 16 45 119 393 5368
Fragments arranged by size:
5368 393 119 45 16
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 2721 4447 2721
Size: 1726 4215
HaeII r_GCGC'y
Cuts at: 1678 5088 1678
Size: 3410 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 2456 2488 2984
Size: 32 135 32 713 32 139 32 496
Cuts at: 2984 3016 4582 4614 4656 4688 1373
Size: 32 1566 32 42 32 2626
Fragments arranged by size:
2626 1566 713 496 139 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2629 3285 3531 669
Size: 988 281 691 656 246 3079
Fragments arranged by size:
3079 988 691 656 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 2412 2455 3266 2412
Size: 43 811 5087
Fragments arranged by size:
5087 811 43
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2331 5055 5239 1305
Size: 789 237 2724 184 2007
Fragments arranged by size:
2724 2007 789 237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2843 10
Size: 54 1425 343 1011 3108
Fragments arranged by size:
3108 1425 1011 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 3119 3899 4258 4417 518
Size: 648 1230 723 780 359 159 2042
Fragments arranged by size:
2042 1230 780 723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2847 2962 3141 3357 4034 5182 5427
Size: 474 767 115 179 216 677 1148 245
Cuts at: 5427 1606
Size: 2120
Fragments arranged by size:
2120 1148 767 677 474 245 216 179
115
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5389
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2509 1348
Size: 1161 4780
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2313 1108
Size: 1205 4736
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2675 2707 4010 4042 5523 5555 2675
Size: 32 1303 32 1481 32 3061
Fragments arranged by size:
3061 1481 1303 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2317 2710 2137
Size: 180 393 5368
Fragments arranged by size:
5368 393 180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2198 2911 2198
Size: 713 5228
PstI C_TGCA'G
Cuts at: 830 2940 3293 830
Size: 2110 353 3478
Fragments arranged by size:
3478 2110 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 2317 2710 2317
Size: 393 5548
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 2168 2240 4189 5901 2168
Size: 72 1949 1712 2208
Fragments arranged by size:
2208 1949 1712 72
SexAI A'CCwGG_T
Cuts at: 2829 2829
Size: 5941
SfcI C'TryA_G
Cuts at: 826 1045 2936 3289 4424 5105 5296 826
Size: 219 1891 353 1135 681 191 1471
Fragments arranged by size:
1891 1471 1135 681 353 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2951 2951
Size: 5941
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 3268 1872
Size: 1396 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 3071 4045 4946 5208 820
Size: 197 926 273 855 974 901 262 1553
Fragments arranged by size:
1553 974 926 901 855 273 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 2807 1517
Size: 1290 4651
StyI C'CwwG_G
Cuts at: 513 1065 3380 513
Size: 552 2315 3074
Fragments arranged by size:
3074 2315 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 2735 3151 3968 3985 4138 4323
Size: 1255 627 528 416 817 17 153 185
Cuts at: 4323 4768 325
Size: 445 1498
Fragments arranged by size:
1498 1255 817 627 528 445 416 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2238 2370
Size: 274 80 33 51 1443 189 72 132
Cuts at: 2370 2976 4187 5899 96
Size: 606 1211 1712 138
Fragments arranged by size:
1712 1443 1211 606 274 189 138 132
80 72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3138 3170 5901 5933 3138
Size: 32 2731 32 3146
Fragments arranged by size:
3146 2731 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2110 1316
Size: 794 5147
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 3266 1870
Size: 1396 4545
XmnI GAAnn'nnTTC
Cuts at: 2687 4070 2687
Size: 1383 4558
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3166 3749 276
Size: 53 83 186 936 1632 583 2468
Fragments arranged by size:
2468 1632 936 583 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SapI SfoI SrfI SwaI XcmI