Restriction map for
pFN21AB5289
(Circular) (Six-base) MAPSORT of: pFN21AB5289.seq Check: 1639 from: 1 to: 5959
pFN21AB5289 5959 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 2363 3280 2363
Size: 917 5042
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2939 2975 4022 5406 5674 1632
Size: 1307 36 1047 1384 268 1917
Fragments arranged by size:
1917 1384 1307 1047 268 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 2014 2058 2174 3702 5274 2014
Size: 44 116 1528 1572 2699
Fragments arranged by size:
2699 1572 1528 116 44
ApaI G_GGCC'C
Cuts at: 3220 3257 3220
Size: 37 5922
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2598 2857 3268 3494 3646 5755 1493
Size: 1105 259 411 226 152 2109 1697
Fragments arranged by size:
2109 1697 1105 411 259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2687 3284 1870
Size: 73 102 642 597 4545
Fragments arranged by size:
4545 642 597 102 73
BamHI G'GATC_C
Cuts at: 2134 3289 3656 2134
Size: 1155 367 4437
Fragments arranged by size:
4437 1155 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 2470 3280 4735 618
Size: 325 217 627 576 107 810 1455 1842
Fragments arranged by size:
1842 1455 810 627 576 325 217 107
BanII G_rGCy'C
Cuts at: 1391 2041 2093 2225 2326 2379 3220 3257 3278
Size: 650 52 132 101 53 841 37 21
Cuts at: 3278 1391
Size: 4072
Fragments arranged by size:
4072 841 650 132 101 53 52 37
21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2927 928
Size: 971 1028 3960
Fragments arranged by size:
3960 1028 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2218 2252 4150 4184 1903
Size: 34 50 34 197 34 1898 34 3678
Fragments arranged by size:
3678 1898 197 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2340 1548
Size: 792 5167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2273 4570
Size: 107 122 71 631 605 337 264 2297
Cuts at: 4570 136
Size: 1525
Fragments arranged by size:
2297 1525 631 605 337 264 122 107
71
BglII A'GATC_T
Cuts at: 2776 5954 2776
Size: 3178 2781
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2052 3220 3257 4020 5176 1790
Size: 262 1168 37 763 1156 2573
Fragments arranged by size:
2573 1168 1156 763 262 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2560 3690 4648
Size: 416 53 83 399 24 1143 1130 958
Cuts at: 4648 442
Size: 1753
Fragments arranged by size:
1753 1143 1130 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2060 2107 2060
Size: 47 5912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2656 4619 1239
Size: 1417 1963 2579
Fragments arranged by size:
2579 1963 1417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 4084 4949 5247 2396
Size: 1688 865 298 3108
Fragments arranged by size:
3108 1688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 2775 5953 2775
Size: 3178 2781
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2810 4392 4790 5064 5211 5622 2810
Size: 1582 398 274 147 411 3147
Fragments arranged by size:
3147 1582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 2033 2099 2141 2144 2238 2888 1186
Size: 829 18 66 42 3 94 650 4257
Fragments arranged by size:
4257 829 650 94 66 42 18 3
BseYI C'CCAG_C
Cuts at: 2284 2796 3122 5162 5658 2284
Size: 512 326 2040 496 2585
Fragments arranged by size:
2585 2040 512 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2576 2948 1163
Size: 573 840 372 4174
Fragments arranged by size:
4174 840 573 372
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2241 2379 4020 4105 5176 2241
Size: 138 1641 85 1071 3024
Fragments arranged by size:
3024 1641 1071 138 85
BsmI GAATG_Cn'
Cuts at: 2616 3470 3563 2616
Size: 854 93 5012
Fragments arranged by size:
5012 854 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2052 2093 2225 2241 2326 2379
Size: 399 251 11 41 132 16 85 53
Cuts at: 2379 3220 3257 3278 4020 4105 5176 1391
Size: 841 37 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 841 742 399 251 132 85
85 53 41 37 21 16 11
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2899 3848 5578 2899
Size: 949 1730 3280
Fragments arranged by size:
3280 1730 949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2541 3314 844
Size: 1697 773 3489
Fragments arranged by size:
3489 1697 773
BsrBI CCG'CTC
Cuts at: 1394 1823 2291 3846 1394
Size: 429 468 1555 3507
Fragments arranged by size:
3507 1555 468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 2467
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2134 2776 3289 3656 4041 4058 5510 5596
Size: 162 642 513 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 642 513 385 367 346 162
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2745 2745
Size: 5959
BtgI C'CryG_G
Cuts at: 513 1065 2262 2359 513
Size: 552 1197 97 4113
Fragments arranged by size:
4113 1197 552 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 2391 2657 3475 4269 4289 2391
Size: 266 818 794 20 4061
Fragments arranged by size:
4061 818 794 266 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 3406 4295
Size: 54 1425 50 275 18 429 1147 889
Cuts at: 4295 5924 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1147 889 429 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2703 3230 3889 1353
Size: 1350 527 659 3423
Fragments arranged by size:
3423 1350 659 527
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2377 2377
Size: 5959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2656 2939 2975 4022 4619 5406 5674
Size: 393 1024 283 36 1047 597 787 268
Cuts at: 5674 1239
Size: 1524
Fragments arranged by size:
1524 1047 1024 787 597 393 283 268
36
EcoO109I rG'GnC_Cy
Cuts at: 2011 2725 3216 3217 3253 3254 2011
Size: 714 491 1 36 1 4716
Fragments arranged by size:
4716 714 491 36 1 1
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 2450 4465 2450
Size: 2015 3944
HaeII r_GCGC'y
Cuts at: 1678 2120 5106 1678
Size: 442 2986 2531
Fragments arranged by size:
2986 2531 442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373
Size: 32 135 32 3028 32 42 32 2626
Fragments arranged by size:
3028 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 2367 3284 2367
Size: 917 5042
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2205 2718 5073 5257 1305
Size: 900 513 2355 184 2007
Fragments arranged by size:
2355 2007 900 513 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 10
Size: 54 1425 343 429 3708
Fragments arranged by size:
3708 1425 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3917 4276 4435 518
Size: 648 2751 359 159 2042
Fragments arranged by size:
2751 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2171 2784 2800 3160 3375 4052 5200
Size: 452 113 613 16 360 215 677 1148
Cuts at: 5200 5445 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 613 452 360 245 215
113 16
NcoI C'CATG_G
Cuts at: 513 1065 2359 513
Size: 552 1294 4113
Fragments arranged by size:
4113 1294 552
NdeI CA'TA_TG
Cuts at: 387 2488 387
Size: 2101 3858
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3128 3249 1348
Size: 1780 121 4058
Fragments arranged by size:
4058 1780 121
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2423 2423
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2725
Size: 5959
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 3216 3253 3216
Size: 37 5922
PspXI vC'TCGA_Gb
Cuts at: 2687 2687
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2994 3138 3311 830
Size: 2164 144 173 3478
Fragments arranged by size:
3478 2164 173 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2058 2784 2800 2058
Size: 726 16 5217
Fragments arranged by size:
5217 726 16
SacI G_AGCT'C
Cuts at: 2379 2379
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SanDI GG'GwC_CC
Cuts at: 2725 2725
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 2994 3311 2994
Size: 317 5642
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2990 3134 3307 4442 5123 5314 826
Size: 219 1945 144 173 1135 681 191 1471
Fragments arranged by size:
1945 1471 1135 681 219 191 173 144
SfiI GGCCn_nnn'nGGCC
Cuts at: 2273 2273
Size: 5959
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 2687 4063 4964 5226 820
Size: 197 926 468 276 1376 901 262 1553
Fragments arranged by size:
1553 1376 926 901 468 276 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2359 3398 513
Size: 552 1294 1039 3074
Fragments arranged by size:
3074 1294 1039 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2542 2925 3986 4003 4156 4341 4786
Size: 1255 962 383 1061 17 153 185 445
Cuts at: 4786 325
Size: 1498
Fragments arranged by size:
1498 1255 1061 962 445 383 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4205 5917 96
Size: 274 80 33 51 1443 2228 1712 138
Fragments arranged by size:
2228 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 2387 1316
Size: 1071 4888
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 2687 1943
Size: 744 5215
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 2680 4088 2680
Size: 1408 4551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI RsrII SacII SapI SexAI
SfoI SrfI SwaI