Restriction map for
pFN21AB5285
(Circular) (Six-base) MAPSORT of: pFN21AB5285.seq Check: 8008 from: 1 to: 6886
pFN21AB5285 6886 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4696 278
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2679 3517 4229 1937
Size: 742 838 712 4594
Fragments arranged by size:
4594 838 742 712
Acc65I G'GTAC_C
Cuts at: 3263 4207 3263
Size: 944 5942
AclI AA'CG_TT
Cuts at: 1563 5013 5386 1563
Size: 3450 373 3063
Fragments arranged by size:
3450 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 2250 2327 2376 2390 2508 4949 6333
Size: 569 49 77 49 14 118 2441 1384
Cuts at: 6333 6601 1632
Size: 268 1917
Fragments arranged by size:
2441 1917 1384 569 268 118 77 49
49 14
AfeI AGC'GCT
Cuts at: 3440 3485 3635 3440
Size: 45 150 6691
Fragments arranged by size:
6691 150 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 2823 2825 3698 5785 6762 1203
Size: 1620 2 873 2087 977 1327
Fragments arranged by size:
2087 1620 1327 977 873 2
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 5615 5615
Size: 6886
AleI CACnn'nnGTG
Cuts at: 1166 2887 1166
Size: 1721 5165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6886
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3000 3032 3000
Size: 32 6854
AlwNI CAG_nnn'CTG
Cuts at: 2267 3585 4085 4629 6201 2267
Size: 1318 500 544 1572 2952
Fragments arranged by size:
2952 1572 1318 544 500
ApaLI G'TGCA_C
Cuts at: 4943 6099 4943
Size: 1156 5730
ApoI r'AATT_y
Cuts at: 1493 2842 3311 4195 4421 4573 6682 1493
Size: 1349 469 884 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 884 469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 4157 4211 1870
Size: 73 2214 54 4545
Fragments arranged by size:
4545 2214 73 54
BamHI G'GATC_C
Cuts at: 4216 4583 4216
Size: 367 6519
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3263 4207 5662 618
Size: 325 217 627 1476 944 1455 1842
Fragments arranged by size:
1842 1476 1455 944 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4092 4205 1391
Size: 2701 113 4072
Fragments arranged by size:
4072 2701 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2231 2801 4032 928
Size: 971 332 570 1231 3782
Fragments arranged by size:
3782 1231 971 570 332
BbvCI CC'TCA_GC
Cuts at: 3918 4169 3918
Size: 251 6635
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5077 5111 1903
Size: 34 3140 34 3678
Fragments arranged by size:
3678 3140 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4778 5988 4778
Size: 1210 5676
BclI T'GATC_A
Cuts at: 1548 3534 1548
Size: 1986 4900
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3747 5497 136
Size: 107 122 71 631 605 2075 1750 1525
Fragments arranged by size:
2075 1750 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3856 6881 3856
Size: 3025 3861
BlpI GC'TnA_GC
Cuts at: 4086 4303 4086
Size: 217 6669
Bme1580I G_kGCm'C
Cuts at: 1790 2246 2978 4947 6103 1790
Size: 456 732 1969 1156 2573
Fragments arranged by size:
2573 1969 1156 732 456
BmgBI CAC'GTC
Cuts at: 1318 3701 1318
Size: 2383 4503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4617 5575 442
Size: 416 53 83 399 24 3200 958 1753
Fragments arranged by size:
3200 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2238 3147 5546 1239
Size: 999 909 2399 2579
Fragments arranged by size:
2579 2399 999 909
Bpu10I CC'TnA_GC
Cuts at: 3906 3918 4169 3906
Size: 12 251 6623
Fragments arranged by size:
6623 251 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5011 5876 6174 5011
Size: 865 298 5723
Fragments arranged by size:
5723 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2872 4140 5549 882
Size: 617 1373 1268 1409 2219
Fragments arranged by size:
2219 1409 1373 1268 617
BsaAI yAC'GTr
Cuts at: 493 1204 2826 4677 493
Size: 711 1622 1851 2702
Fragments arranged by size:
2702 1851 1622 711
BsaBI GATnn'nnATC
Cuts at: 6880 6880
Size: 6886
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2086 4693 5075 5710
Size: 53 83 186 936 553 2607 382 635
Cuts at: 5710 275
Size: 1451
Fragments arranged by size:
2607 1451 936 635 553 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5319 5717 5991 6138 6549 5319
Size: 398 274 147 411 5656
Fragments arranged by size:
5656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2236 2266 3000 3030 1178
Size: 30 165 30 833 30 734 30 5034
Fragments arranged by size:
5034 833 734 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2666 3783 3893 3896 4124 1186
Size: 865 615 1117 110 3 228 3948
Fragments arranged by size:
3948 1117 865 615 228 110 3
BseYI C'CCAG_C
Cuts at: 3931 4116 6089 6585 3931
Size: 185 1973 496 4232
Fragments arranged by size:
4232 1973 496 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3166 3175 1163
Size: 573 1430 9 4874
Fragments arranged by size:
4874 1430 573 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4336 5097 6125 1540
Size: 275 186 2335 761 1028 2301
Fragments arranged by size:
2335 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4947 5032 6103 4947
Size: 85 1071 5730
Fragments arranged by size:
5730 1071 85
BsmI GAATG_Cn'
Cuts at: 2909 3382 4397 4490 2909
Size: 473 1015 93 5305
Fragments arranged by size:
5305 1015 473 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2077 2077
Size: 6886
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2246 2978 4092 4205 4947 5032 6103
Size: 399 456 732 1114 113 742 85 1071
Cuts at: 6103 1391
Size: 2174
Fragments arranged by size:
2174 1114 1071 742 732 456 399 113
85
BspEI T'CCGG_A
Cuts at: 6549 6549
Size: 6886
BspHI T'CATG_A
Cuts at: 3433 4775 6505 3433
Size: 1342 1730 3814
Fragments arranged by size:
3814 1730 1342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4241 844
Size: 3397 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4773 1394
Size: 429 2950 3507
Fragments arranged by size:
3507 2950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5381 5555 65
Size: 1183 4133 174 1396
Fragments arranged by size:
4133 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5530 5717 1700
Size: 110 3720 187 2869
Fragments arranged by size:
3720 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2176 96
Size: 2080 4806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 3044 4946 5958 3044
Size: 1902 1012 3972
Fragments arranged by size:
3972 1902 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2267 3153 3408 3585 3722 794
Size: 1473 886 255 177 137 3958
Fragments arranged by size:
3958 1473 886 255 177 137
BstBI TT'CG_AA
Cuts at: 5690 5690
Size: 6886
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6886
BstXI CCAn_nnnn'nTGG
Cuts at: 2141 2900 2141
Size: 759 6127
BstYI r'GATC_y
Cuts at: 1972 2566 3856 4216 4583 4968 4985 6437 6523
Size: 594 1290 360 367 385 17 1452 86
Cuts at: 6523 6535 6881 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1290 594 385 367 360 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2930 2930
Size: 6886
BtgI C'CryG_G
Cuts at: 513 1065 2004 2831 3711 513
Size: 552 939 827 880 3688
Fragments arranged by size:
3688 939 880 827 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6147
Fragments arranged by size:
6147 683 56
BtsI GCAGTG_nn'
Cuts at: 2179 3583 4402 5196 5216 2179
Size: 1404 819 794 20 3849
Fragments arranged by size:
3849 1404 819 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6851
DraI TTT'AAA
Cuts at: 4190 4190
Size: 6886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3306 4333 5222
Size: 54 1425 50 275 18 1476 1027 889
Cuts at: 5222 6851 8
Size: 1629 43
Fragments arranged by size:
1629 1476 1425 1027 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3776 4101 4816 1353
Size: 2423 325 715 3423
Fragments arranged by size:
3423 2423 715 325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3590 5473 5847 5993 387
Size: 1064 138 2001 1883 374 146 1280
Fragments arranged by size:
2001 1883 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2238 2250 2327 2376 2390 2508
Size: 393 569 37 12 77 49 14 118
Cuts at: 2508 3147 4949 5546 6333 6601 1239
Size: 639 1802 597 787 268 1524
Fragments arranged by size:
1802 1524 787 639 597 569 393 268
118 77 49 37 14 12
EcoO109I rG'GnC_Cy
Cuts at: 2440 2440
Size: 6886
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2459 2567 2459
Size: 108 6778
FspI TGC'GCA
Cuts at: 5392 5392
Size: 6886
HaeII r_GCGC'y
Cuts at: 1678 3442 3487 3637 6033 1678
Size: 1764 45 150 2396 2531
Fragments arranged by size:
2531 2396 1764 150 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5527 5559 5601 5633 1373
Size: 32 135 32 3955 32 42 32 2626
Fragments arranged by size:
3955 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2167 4230 4476 669
Size: 988 281 229 2063 246 3079
Fragments arranged by size:
3079 2063 988 281 246 229
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6886
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KpnI G_GTAC'C
Cuts at: 3267 4211 3267
Size: 944 5942
MfeI C'AATT_G
Cuts at: 4044 4485 4044
Size: 441 6445
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3683 6000 6184 1305
Size: 2378 2317 184 2007
Fragments arranged by size:
2378 2317 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3308 10
Size: 54 1425 343 1476 3588
Fragments arranged by size:
3588 1476 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2803 2871 2887 2898 3058 3272 3474
Size: 648 1637 68 16 11 160 214 202
Cuts at: 3474 3536 4844 5203 5362 518
Size: 62 1308 359 159 2042
Fragments arranged by size:
2042 1637 1308 648 359 214 202 160
159 68 62 16 11
MspA1I CmG'CkG
Cuts at: 1606 2200 4124 4302 4979 6127 6372 1606
Size: 594 1924 178 677 1148 245 2120
Fragments arranged by size:
2120 1924 1148 677 594 245 178
NcoI C'CATG_G
Cuts at: 513 1065 2004 3711 513
Size: 552 939 1707 3688
Fragments arranged by size:
3688 1707 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 3625 3725 4244 5789 1132
Size: 97 2396 100 519 1545 2229
Fragments arranged by size:
2396 2229 1545 519 100 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6886
PciI A'CATG_T
Cuts at: 5785 5785
Size: 6886
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6886
PfoI T'CCnGG_A
Cuts at: 2157 2157
Size: 6886
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PmlI CAC'GTG
Cuts at: 2826 2826
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3000 3032 4955 4987 6468 6500 3000
Size: 32 1923 32 1481 32 3386
Fragments arranged by size:
3386 1923 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6886
PsiI TTA'TAA
Cuts at: 2291 4456 2291
Size: 2165 4721
PstI C_TGCA'G
Cuts at: 830 3581 4238 830
Size: 2751 657 3478
Fragments arranged by size:
3478 2751 657
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6886
PvuII CAG'CTG
Cuts at: 2200 4124 2200
Size: 1924 4962
SalI G'TCGA_C
Cuts at: 1936 4228 1936
Size: 2292 4594
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 5134 6846 5134
Size: 1712 5174
SfcI C'TryA_G
Cuts at: 826 1045 3577 4234 5369 6050 6241 826
Size: 219 2532 657 1135 681 191 1471
Fragments arranged by size:
2532 1471 1135 681 657 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 4213 1872
Size: 2341 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4990 5891 6153 820
Size: 197 926 3047 901 262 1553
Fragments arranged by size:
3047 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4677 493
Size: 4184 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 4244 1132
Size: 3112 3774
SspI AAT'ATT
Cuts at: 5 52 4810 5
Size: 47 4758 2081
Fragments arranged by size:
4758 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6886
StyI C'CwwG_G
Cuts at: 513 1065 2004 3711 4325 513
Size: 552 939 1707 614 3074
Fragments arranged by size:
3074 1707 939 614 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3056 3350 3566 4913 4930 5083 5268
Size: 1255 1476 294 216 1347 17 153 185
Cuts at: 5268 5713 325
Size: 445 1498
Fragments arranged by size:
1498 1476 1347 1255 445 294 216 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2176 5132 6844
Size: 274 80 33 51 1443 199 2956 1712
Cuts at: 6844 96
Size: 138
Fragments arranged by size:
2956 1712 1443 274 199 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2236 2268 6846 6878 2236
Size: 32 4578 32 2244
Fragments arranged by size:
4578 2244 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3335 1316
Size: 2019 4867
XbaI T'CTAG_A
Cuts at: 4222 4222
Size: 6886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2524 3010 2524
Size: 486 6400
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6886
XmaI C'CCGG_G
Cuts at: 1870 4211 1870
Size: 2341 4545
XmnI GAAnn'nnTTC
Cuts at: 2403 2493 2798 3015 3053 3205 5015 2403
Size: 90 305 217 38 152 1810 4274
Fragments arranged by size:
4274 1810 305 217 152 90 38
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4694 276
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI