Restriction map for
pFN21AB5282
(Circular) (Six-base) MAPSORT of: pFN21AB5282.seq Check: 966 from: 1 to: 5380
pFN21AB5282 5380 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 1937
Size: 786 4594
Acc65I G'GTAC_C
Cuts at: 2701 2701
Size: 5380
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2605 3443 4827 5095 1632
Size: 973 838 1384 268 1917
Fragments arranged by size:
1917 1384 973 838 268
AflII C'TTAA_G
Cuts at: 820 1017 2270 820
Size: 197 1253 3930
Fragments arranged by size:
3930 1253 197
AflIII A'CryG_T
Cuts at: 1203 4279 5256 1203
Size: 3076 977 1327
Fragments arranged by size:
3076 1327 977
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 4109 4109
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5380
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2397 2429 2397
Size: 32 5348
AlwNI CAG_nnn'CTG
Cuts at: 3123 4695 3123
Size: 1572 3808
ApaLI G'TGCA_C
Cuts at: 3437 4593 3437
Size: 1156 4224
ApoI r'AATT_y
Cuts at: 1493 2083 2243 2287 2689 2915 3067 5176 1493
Size: 590 160 44 402 226 152 2109 1697
Fragments arranged by size:
2109 1697 590 402 226 160 152 44
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2705 1870
Size: 73 762 4545
Fragments arranged by size:
4545 762 73
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2701 4156 618
Size: 325 217 627 239 675 1455 1842
Fragments arranged by size:
1842 1455 675 627 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2699 1391
Size: 1308 4072
BbeI G_GCGC'C
Cuts at: 2030 2030
Size: 5380
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3571 3605 1903
Size: 34 1634 34 3678
Fragments arranged by size:
3678 1634 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3272 4482 3272
Size: 1210 4170
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3991 136
Size: 107 122 71 631 605 2319 1525
Fragments arranged by size:
2319 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2626 5375 2626
Size: 2749 2631
BlpI GC'TnA_GC
Cuts at: 2797 2797
Size: 5380
Bme1580I G_kGCm'C
Cuts at: 1790 3441 4597 1790
Size: 1651 1156 2573
Fragments arranged by size:
2573 1651 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 2451 3111
Size: 416 53 83 399 24 711 323 660
Cuts at: 3111 4069 442
Size: 958 1753
Fragments arranged by size:
1753 958 711 660 416 399 323 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4040 1239
Size: 2801 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 4370 4668 3505
Size: 865 298 4217
Fragments arranged by size:
4217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4043 882
Size: 617 2544 2219
Fragments arranged by size:
2544 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2094 3171 493
Size: 711 890 1077 2702
Fragments arranged by size:
2702 1077 890 711
BsaBI GATnn'nnATC
Cuts at: 2631 5374 2631
Size: 2743 2637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2046 3187 3569
Size: 53 83 186 936 494 19 1141 382
Cuts at: 3569 4204 275
Size: 635 1451
Fragments arranged by size:
1451 1141 936 635 494 382 186 83
53 19
BsaWI w'CCGG_w
Cuts at: 3813 4211 4485 4632 5043 3813
Size: 398 274 147 411 4150
Fragments arranged by size:
4150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5155
Fragments arranged by size:
5155 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 1186
Size: 850 4530
BseYI C'CCAG_C
Cuts at: 4583 5079 4583
Size: 496 4884
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4807
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2830 3591 4619 1540
Size: 275 186 829 761 1028 2301
Fragments arranged by size:
2301 1028 829 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2658 3441 3526 4597 2658
Size: 783 85 1071 3441
Fragments arranged by size:
3441 1071 783 85
BsmI GAATG_Cn'
Cuts at: 2891 2984 2891
Size: 93 5287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2658 2699 3441 3526 4597 1391
Size: 399 868 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 868 742 399 85 41
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 3269 4999 3269
Size: 1730 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 844
Size: 1891 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 3267 1394
Size: 429 344 1100 3507
Fragments arranged by size:
3507 1100 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 4049 65
Size: 1183 2627 174 1396
Fragments arranged by size:
2627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4024 4211 1700
Size: 110 2214 187 2869
Fragments arranged by size:
2869 2214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5380
BssSI C'ACGA_G
Cuts at: 3440 4452 3440
Size: 1012 4368
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5380
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5380
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 5380
BstYI r'GATC_y
Cuts at: 1972 2626 2710 3077 3462 3479 4931 5017 5029
Size: 654 84 367 385 17 1452 86 12
Cuts at: 5029 5375 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 654 385 367 346 86 84
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3538
Fragments arranged by size:
3538 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2397 487
Size: 683 56 1171 3470
Fragments arranged by size:
3470 1171 683 56
BtsI GCAGTG_nn'
Cuts at: 2896 3690 3710 2896
Size: 794 20 4566
Fragments arranged by size:
4566 794 20
ClaI AT'CG_AT
Cuts at: 1554 2296 1554
Size: 742 4638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5345
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2213 4387 809
Size: 507 897 2174 1802
Fragments arranged by size:
2174 1802 897 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2066 2172
Size: 54 1425 50 275 18 177 59 106
Cuts at: 2172 2827 3716 5345 8
Size: 655 889 1629 43
Fragments arranged by size:
1629 1425 889 655 275 177 106 59
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2827 1537
Size: 275 1015 4090
Fragments arranged by size:
4090 1015 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2516 3310 1353
Size: 1163 794 3423
Fragments arranged by size:
3423 1163 794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3967 4341 4487 387
Size: 1064 138 2378 374 146 1280
Fragments arranged by size:
2378 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2605 3443 4040 4827 5095 1239
Size: 393 973 838 597 787 268 1524
Fragments arranged by size:
1524 973 838 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2689 3067 2689
Size: 378 5002
FspI TGC'GCA
Cuts at: 3886 3886
Size: 5380
HaeII r_GCGC'y
Cuts at: 1678 2030 4527 1678
Size: 352 2497 2531
Fragments arranged by size:
2531 2497 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4021 4053 4095 4127 1373
Size: 32 135 32 2449 32 42 32 2626
Fragments arranged by size:
2626 2449 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2724 2970 669
Size: 988 281 786 246 3079
Fragments arranged by size:
3079 988 786 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KasI G'GCGC_C
Cuts at: 2026 2026
Size: 5380
KpnI G_GTAC'C
Cuts at: 2705 2705
Size: 5380
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 5256 5256
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2416 4494 4678 1305
Size: 1111 2078 184 2007
Fragments arranged by size:
2078 2007 1111 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2068 2174 10
Size: 54 1425 343 236 106 3216
Fragments arranged by size:
3216 1425 343 236 106 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2109 3338 3697 3856 518
Size: 648 943 1229 359 159 2042
Fragments arranged by size:
2042 1229 943 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2796 3473 4621 4866 1606
Size: 1190 677 1148 245 2120
Fragments arranged by size:
2120 1190 1148 677 245
NarI GG'CG_CC
Cuts at: 2027 2027
Size: 5380
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3538
Fragments arranged by size:
3538 939 552 351
NdeI CA'TA_TG
Cuts at: 387 2639 387
Size: 2252 3128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2738 4283 1132
Size: 97 1509 1545 2229
Fragments arranged by size:
2229 1545 1509 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5380
PciI A'CATG_T
Cuts at: 4279 4279
Size: 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2356 1108
Size: 1248 4132
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PstI C_TGCA'G
Cuts at: 830 2732 830
Size: 1902 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SfcI C'TryA_G
Cuts at: 826 1045 2728 3863 4544 4735 826
Size: 219 1683 1135 681 191 1471
Fragments arranged by size:
1683 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2028 2028
Size: 5380
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2707 1872
Size: 835 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2270 3484 4385 4647 820
Size: 197 926 327 1214 901 262 1553
Fragments arranged by size:
1553 1214 926 901 327 262 197
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2738 1132
Size: 1606 3774
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5380
StyI C'CwwG_G
Cuts at: 513 1065 2004 2175 2355 2819 513
Size: 552 939 171 180 464 3074
Fragments arranged by size:
3074 939 552 464 180 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2348 3407 3424 3577 3762 4207 325
Size: 1255 768 1059 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1059 768 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 5338 96
Size: 274 80 33 51 1443 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5340 5372 5340
Size: 32 5348
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2705 1870
Size: 835 4545
XmnI GAAnn'nnTTC
Cuts at: 2087 3509 2087
Size: 1422 3958
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI