Restriction map for
pFN21AB5278
(Circular) (Six-base) MAPSORT of: pFN21AB5278.seq Check: 3204 from: 1 to: 5830
pFN21AB5278 5830 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2733 3173 1937
Size: 796 440 4594
Fragments arranged by size:
4594 796 440
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2262 3893 5277 5545 1632
Size: 630 1631 1384 268 1917
Fragments arranged by size:
1917 1631 1384 630 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 2786 2943 1166
Size: 1620 157 4053
Fragments arranged by size:
4053 1620 157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2051 1184
Size: 867 4963
AlwNI CAG_nnn'CTG
Cuts at: 3573 5145 3573
Size: 1572 4258
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 2566 3139 3365 3517 5626 1493
Size: 1073 573 226 152 2109 1697
Fragments arranged by size:
2109 1697 1073 573 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 2633 2784 3155 1870
Size: 73 690 151 371 4545
Fragments arranged by size:
4545 690 371 151 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2639 3151 4606 618
Size: 325 217 627 852 512 1455 1842
Fragments arranged by size:
1842 1455 852 627 512 325 217
BanII G_rGCy'C
Cuts at: 1391 2802 3149 1391
Size: 1411 347 4072
Fragments arranged by size:
4072 1411 347
BbeI G_GCGC'C
Cuts at: 2643 2643
Size: 5830
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3109 3143 4021 4055 1903
Size: 34 1172 34 878 34 3678
Fragments arranged by size:
3678 1172 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 2409 1548
Size: 861 4969
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2886 4441 136
Size: 107 122 71 631 605 1214 1555 1525
Fragments arranged by size:
1555 1525 1214 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2793 3891 5047 1790
Size: 1003 1098 1156 2573
Fragments arranged by size:
2573 1156 1098 1003
BmgBI CAC'GTC
Cuts at: 1318 2599 1318
Size: 1281 4549
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2072 3561 4519
Size: 416 53 83 399 24 655 1489 958
Cuts at: 4519 442
Size: 1753
Fragments arranged by size:
1753 1489 958 655 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2042 1056
Size: 986 4844
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 4490 1239
Size: 848 2403 2579
Fragments arranged by size:
2579 2403 848
Bpu10I CC'TnA_GC
Cuts at: 2655 2655
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3008 4493 882
Size: 617 1509 1485 2219
Fragments arranged by size:
2219 1509 1485 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 2813 5824 2813
Size: 3011 2819
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2640 3637 4019 4654
Size: 53 83 186 936 1107 997 382 635
Cuts at: 4654 275
Size: 1451
Fragments arranged by size:
1451 1107 997 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2085 2115 1178
Size: 30 165 30 682 30 4893
Fragments arranged by size:
4893 682 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 1186
Size: 940 4890
BseYI C'CCAG_C
Cuts at: 5033 5529 5033
Size: 496 5334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2987 1163
Size: 573 1251 4006
Fragments arranged by size:
4006 1251 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2987 3891 3976 5047 2987
Size: 904 85 1071 3770
Fragments arranged by size:
3770 1071 904 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2793 2802 2987 3149 3891 3976 5047
Size: 399 1003 9 185 162 742 85 1071
Cuts at: 5047 1391
Size: 2174
Fragments arranged by size:
2174 1071 1003 742 399 185 162 85
9
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 844
Size: 2341 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2503 4325 4499 65
Size: 1183 1255 1822 174 1396
Fragments arranged by size:
1822 1396 1255 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2690 4474 4661 1700
Size: 110 880 1784 187 2869
Fragments arranged by size:
2869 1784 880 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3890 4902 3890
Size: 1012 4818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5830
BstBI TT'CG_AA
Cuts at: 2812 4634 2812
Size: 1822 4008
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2332 2128
Size: 204 5626
BstYI r'GATC_y
Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825
Size: 1188 367 385 17 1452 86 12 346
Cuts at: 5825 1972
Size: 1977
Fragments arranged by size:
1977 1452 1188 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2928 513
Size: 552 939 924 3415
Fragments arranged by size:
3415 939 924 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 2615 2920 2928 3127 3346 4140 4160 2615
Size: 305 8 199 219 794 20 4285
Fragments arranged by size:
4285 794 305 219 199 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2292 2778 3277
Size: 54 1425 50 275 18 462 486 499
Cuts at: 3277 4166 5795 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 499 486 462 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3760 1353
Size: 2407 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2262 3893 4490 5277 5545 1239
Size: 393 455 175 1631 597 787 268 1524
Fragments arranged by size:
1631 1524 787 597 455 393 268 175
EcoRI G'AATT_C
Cuts at: 2566 3139 3517 2566
Size: 573 378 4879
Fragments arranged by size:
4879 573 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 5830
FspI TGC'GCA
Cuts at: 2027 2621 4336 2027
Size: 594 1715 3521
Fragments arranged by size:
3521 1715 594
HaeII r_GCGC'y
Cuts at: 1678 2643 4977 1678
Size: 965 2334 2531
Fragments arranged by size:
2531 2334 965
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2380 2412 4471 4503 4545
Size: 32 135 32 808 32 2059 32 42
Cuts at: 4545 4577 1373
Size: 32 2626
Fragments arranged by size:
2626 2059 808 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KasI G'GCGC_C
Cuts at: 2639 2639
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2780 10
Size: 54 1425 343 948 3060
Fragments arranged by size:
3060 1425 948 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 2555 2786 2943 3009 3788 4147
Size: 648 1323 66 231 157 66 779 359
Cuts at: 4147 4306 518
Size: 159 2042
Fragments arranged by size:
2042 1323 779 648 359 231 159 157
66 66
MspA1I CmG'CkG
Cuts at: 1606 2150 2472 2481 2650 3246 3923 5071 5316
Size: 544 322 9 169 596 677 1148 245
Cuts at: 5316 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 596 544 322 245 169
9
NaeI GCC'GGC
Cuts at: 2692 2692
Size: 5830
NarI GG'CG_CC
Cuts at: 2640 2640
Size: 5830
NcoI C'CATG_G
Cuts at: 513 1065 2004 2928 513
Size: 552 939 924 3415
Fragments arranged by size:
3415 939 924 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NgoMIV G'CCGG_C
Cuts at: 2690 2690
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 2038 1052
Size: 986 4844
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 3188 4733 1132
Size: 97 1959 1545 2229
Fragments arranged by size:
2229 1959 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PfoI T'CCnGG_A
Cuts at: 2331 2364 2835 3044 2331
Size: 33 471 209 5117
Fragments arranged by size:
5117 471 209 33
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspXI vC'TCGA_Gb
Cuts at: 2784 2784
Size: 5830
PstI C_TGCA'G
Cuts at: 830 3182 830
Size: 2352 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 3178 4313 4994 5185 826
Size: 219 2133 1135 681 191 1471
Fragments arranged by size:
2133 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2886 2886
Size: 5830
SfoI GGC'GCC
Cuts at: 2641 2641
Size: 5830
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2784 3934 4835 5097 820
Size: 197 926 841 1150 901 262 1553
Fragments arranged by size:
1553 1150 926 901 841 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 2004 2457 2793 2908 2928 3269 513
Size: 552 939 453 336 115 20 341 3074
Fragments arranged by size:
3074 939 552 453 341 336 115 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 3004 3857 3874 4027 4212 4657
Size: 1255 758 666 853 17 153 185 445
Cuts at: 4657 325
Size: 1498
Fragments arranged by size:
1498 1255 853 758 666 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4076 5788 96
Size: 274 80 33 51 1443 2099 1712 138
Fragments arranged by size:
2099 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2313 2345 2699 2731 5790 5822 2313
Size: 32 354 32 3059 32 2321
Fragments arranged by size:
3059 2321 354 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2521 2011
Size: 510 5320
XhoI C'TCGA_G
Cuts at: 1943 2784 1943
Size: 841 4989
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI NruI NsiI PacI PmlI
PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SrfI SwaI