Restriction map for
pFN21AB5274
(Circular) (Six-base) MAPSORT of: pFN21AB5274.seq Check: 9416 from: 1 to: 5752
pFN21AB5274 5752 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2208 3095 1937
Size: 271 887 4594
Fragments arranged by size:
4594 887 271
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2547 3815 5199 5467 1632
Size: 915 1268 1384 268 1917
Fragments arranged by size:
1917 1384 1268 915 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 2525 3495 5067 2525
Size: 970 1572 3210
Fragments arranged by size:
3210 1572 970
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2844 3061 3287 3439 5548 1493
Size: 1351 217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1351 226 217 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 3077 1870
Size: 73 1134 4545
Fragments arranged by size:
4545 1134 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2979 3071 1391
Size: 1588 92 4072
Fragments arranged by size:
4072 1588 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2459 2997 928
Size: 971 560 538 3683
Fragments arranged by size:
3683 971 560 538
BbvCI CC'TCA_GC
Cuts at: 2681 2681
Size: 5752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2302 3644 4854 2302
Size: 1342 1210 3200
Fragments arranged by size:
3200 1342 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4363 136
Size: 107 122 71 631 605 2691 1525
Fragments arranged by size:
2691 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2758 5747 2758
Size: 2989 2763
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 3813 4969 1790
Size: 2023 1156 2573
Fragments arranged by size:
2573 2023 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2343 1056
Size: 1287 4465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 2060 2466 2592 2783 4412 1239
Size: 813 8 406 126 191 1629 2579
Fragments arranged by size:
2579 1629 813 406 191 126 8
Bpu10I CC'TnA_GC
Cuts at: 2681 2681
Size: 5752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2930 3877 4742 5040 2105
Size: 825 947 865 298 2817
Fragments arranged by size:
2817 947 865 825 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2702 2879 4415 882
Size: 617 1203 177 1536 2219
Fragments arranged by size:
2219 1536 1203 617 177
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 2307 2313 5746 2307
Size: 6 3433 2313
Fragments arranged by size:
3433 2313 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2158 3559 3941 4576
Size: 53 83 186 936 625 1401 382 635
Cuts at: 4576 275
Size: 1451
Fragments arranged by size:
1451 1401 936 635 625 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2448 4185 4583 4857 5004 5415 2448
Size: 1737 398 274 147 411 2785
Fragments arranged by size:
2785 1737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 1178
Size: 30 165 30 630 30 4867
Fragments arranged by size:
4867 630 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2019 2342 3041 1186
Size: 833 323 699 3897
Fragments arranged by size:
3897 833 699 323
BseYI C'CCAG_C
Cuts at: 2712 4955 5451 2712
Size: 2243 496 3013
Fragments arranged by size:
3013 2243 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2979 3813 3898 4969 2979
Size: 834 85 1071 3762
Fragments arranged by size:
3762 1071 834 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2979 3071 3813 3898 4969 1391
Size: 399 1189 92 742 85 1071 2174
Fragments arranged by size:
2174 1189 1071 742 399 92 85
BspEI T'CCGG_A
Cuts at: 2448 5415 2448
Size: 2967 2785
BspHI T'CATG_A
Cuts at: 2922 3641 5371 2922
Size: 719 1730 3303
Fragments arranged by size:
3303 1730 719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 3107 844
Size: 1376 887 3489
Fragments arranged by size:
3489 1376 887
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 2833
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2758 2914 3082 3449 3834 3851 5303 5389
Size: 786 156 168 367 385 17 1452 86
Cuts at: 5389 5401 5747 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 786 385 367 346 168 156
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2404 513
Size: 552 1339 3861
Fragments arranged by size:
3861 1339 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 2698 3268 4062 4082 2698
Size: 570 794 20 4368
Fragments arranged by size:
4368 794 570 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3199 4088 5717
Size: 54 1425 50 275 18 1369 889 1629
Cuts at: 5717 8
Size: 43
Fragments arranged by size:
1629 1425 1369 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2432 2608 3682 1353
Size: 1079 176 1074 3423
Fragments arranged by size:
3423 1079 1074 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2977 2977
Size: 5752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2060 2466 2547 2592 2783 3815
Size: 393 420 8 406 81 45 191 1032
Cuts at: 3815 4412 5199 5467 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1032 787 597 420 406 393 268
191 81 45 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3047 3047
Size: 5752
EcoRI G'AATT_C
Cuts at: 2844 3061 3439 2844
Size: 217 378 5157
Fragments arranged by size:
5157 378 217
EcoRV GAT'ATC
Cuts at: 2275 2599 2275
Size: 324 5428
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 4899 1678
Size: 3221 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2701 2733 3020 3052 4393
Size: 32 135 32 1129 32 287 32 1341
Cuts at: 4393 4425 4467 4499 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1341 1129 287 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2116 3096 3342 669
Size: 988 281 178 980 246 3079
Fragments arranged by size:
3079 988 980 281 246 178
HindIII A'AGCT_T
Cuts at: 748 2230 748
Size: 1482 4270
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 2136 3351 2136
Size: 1215 4537
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2151 2721 2927 3710 4069 4228 518
Size: 648 985 570 206 783 359 159 2042
Fragments arranged by size:
2042 985 783 648 570 359 206 159
MspA1I CmG'CkG
Cuts at: 1606 2270 2833 3168 3845 4993 5238 1606
Size: 664 563 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 563 335 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5200
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 2339 1052
Size: 1287 4465
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3110 4655 1132
Size: 97 1881 1545 2229
Fragments arranged by size:
2229 1881 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PfoI T'CCnGG_A
Cuts at: 2608 2608
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2270 2833 2270
Size: 563 5189
SacI G_AGCT'C
Cuts at: 2979 2979
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 2539 4000 5712 2539
Size: 1461 1712 2579
Fragments arranged by size:
2579 1712 1461
SfcI C'TryA_G
Cuts at: 826 1045 2774 3100 4235 4916 5107 826
Size: 219 1729 326 1135 681 191 1471
Fragments arranged by size:
1729 1471 1135 681 326 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 2945 3856 4757 5019 820
Size: 197 926 177 825 911 901 262 1553
Fragments arranged by size:
1553 926 911 901 825 262 197 177
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 2592 1517
Size: 1075 4677
StyI C'CwwG_G
Cuts at: 513 1065 3191 513
Size: 552 2126 3074
Fragments arranged by size:
3074 2126 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3009 3779 3796 3949 4134 4579 325
Size: 1255 1429 770 17 153 185 445 1498
Fragments arranged by size:
1498 1429 1255 770 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2098 2516 2537
Size: 274 80 33 51 1443 121 418 21
Cuts at: 2537 2965 3998 5710 96
Size: 428 1033 1712 138
Fragments arranged by size:
1712 1443 1033 428 418 274 138 121
80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI