Restriction map for
pFN21AB5268
(Circular) (Six-base) MAPSORT of: pFN21AB5268.seq Check: 3988 from: 1 to: 5809
pFN21AB5268 5809 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3152 1937
Size: 1215 4594
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3872 5256 5524 1632
Size: 2240 1384 268 1917
Fragments arranged by size:
2240 1917 1384 268
AfeI AGC'GCT
Cuts at: 2736 2736
Size: 5809
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 2408 1166
Size: 1242 4567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5809
AlwNI CAG_nnn'CTG
Cuts at: 3552 5124 3552
Size: 1572 4237
ApaI G_GGCC'C
Cuts at: 2258 2517 2536 3014 2258
Size: 259 19 478 5053
Fragments arranged by size:
5053 478 259 19
ApaLI G'TGCA_C
Cuts at: 2357 2925 3866 5022 2357
Size: 568 941 1156 3144
Fragments arranged by size:
3144 1156 941 568
ApoI r'AATT_y
Cuts at: 1493 3118 3344 3496 5605 1493
Size: 1625 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 2315 2510 2708 3050 3134 1870
Size: 73 288 84 195 198 342 84 4545
Fragments arranged by size:
4545 342 288 198 195 84 84 73
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2573 2791 2967 3130 4585
Size: 325 217 627 786 218 176 163 1455
Cuts at: 4585 618
Size: 1842
Fragments arranged by size:
1842 1455 786 627 325 218 217 176
163
BanII G_rGCy'C
Cuts at: 1391 2258 2517 2536 3014 3074 3128 1391
Size: 867 259 19 478 60 54 4072
Fragments arranged by size:
4072 867 478 259 60 54 19
BbeI G_GCGC'C
Cuts at: 2577 2795 2577
Size: 218 5591
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4000 4034 1903
Size: 34 2063 34 3678
Fragments arranged by size:
3678 2063 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3701 4911 3701
Size: 1210 4599
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2233 2414 2521
Size: 107 122 71 631 605 561 181 107
Cuts at: 2521 2540 2626 2695 2822 2846 2855 4420 136
Size: 19 86 69 127 24 9 1565 1525
Fragments arranged by size:
1565 1525 631 605 561 181 127 122
107 107 86 71 69 24 19 9
BglII A'GATC_T
Cuts at: 5804 5804
Size: 5809
BlpI GC'TnA_GC
Cuts at: 3226 3226
Size: 5809
Bme1580I G_kGCm'C
Cuts at: 1790 2089 2258 2361 2517 2536 2929 2980 3014
Size: 299 169 103 156 19 393 51 34
Cuts at: 3014 3870 5026 1790
Size: 856 1156 2573
Fragments arranged by size:
2573 1156 856 393 299 169 156 103
51 34 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2915 3540 4498
Size: 416 53 83 399 24 1498 625 958
Cuts at: 4498 442
Size: 1753
Fragments arranged by size:
1753 1498 958 625 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2978 3021 4469 1239
Size: 1739 43 1448 2579
Fragments arranged by size:
2579 1739 1448 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3934 4799 5097 3934
Size: 865 298 4646
Fragments arranged by size:
4646 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2992 4472 882
Size: 617 1493 1480 2219
Fragments arranged by size:
2219 1493 1480 617
BsaAI yAC'GTr
Cuts at: 493 1204 3600 493
Size: 711 2396 2702
Fragments arranged by size:
2702 2396 711
BsaBI GATnn'nnATC
Cuts at: 5803 5803
Size: 5809
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2574 2792 3616 3998
Size: 53 83 186 936 1041 218 824 382
Cuts at: 3998 4633 275
Size: 635 1451
Fragments arranged by size:
1451 1041 936 824 635 382 218 186
83 53
BsaWI w'CCGG_w
Cuts at: 4242 4640 4914 5061 5472 4242
Size: 398 274 147 411 4579
Fragments arranged by size:
4579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5584
Fragments arranged by size:
5584 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5809
BseYI C'CCAG_C
Cuts at: 2092 2206 2236 2431 2501 2731 5012 5508 2092
Size: 114 30 195 70 230 2281 496 2393
Fragments arranged by size:
2393 2281 496 230 195 114 70 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2293 2935 1163
Size: 573 557 642 4037
Fragments arranged by size:
4037 642 573 557
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2190 2384 2525 2630 3259 4020
Size: 275 186 189 194 141 105 629 761
Cuts at: 4020 5048 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 629 275 194 189 186
141 105
BsiHKAI G_wGCw'C
Cuts at: 2361 2397 2403 2929 3870 3955 5026 2361
Size: 36 6 526 941 85 1071 3144
Fragments arranged by size:
3144 1071 941 526 85 36 6
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2258 2361 2397 2403 2517 2536
Size: 399 299 169 103 36 6 114 19
Cuts at: 2536 2929 2980 3014 3074 3128 3870 3955 5026
Size: 393 51 34 60 54 742 85 1071
Cuts at: 5026 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 393 299 169 114
103 85 60 54 51 36 34 19
6
BspEI T'CCGG_A
Cuts at: 5472 5472
Size: 5809
BspHI T'CATG_A
Cuts at: 3698 5428 3698
Size: 1730 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 3164 844
Size: 1343 977 3489
Fragments arranged by size:
3489 1343 977
BsrBI CCG'CTC
Cuts at: 1394 1823 2276 2294 2527 2652 2692 3696 1394
Size: 429 453 18 233 125 40 1004 3507
Fragments arranged by size:
3507 1004 453 429 233 125 40 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 2993 4304 4478 65
Size: 1183 1745 1311 174 1396
Fragments arranged by size:
1745 1396 1311 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2302 2629 4453 4640 1700
Size: 110 492 327 1824 187 2869
Fragments arranged by size:
2869 1824 492 327 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 2103 2436 2633 1925
Size: 178 333 197 5101
Fragments arranged by size:
5101 333 197 178
BssSI C'ACGA_G
Cuts at: 2396 3869 4881 2396
Size: 1473 1012 3324
Fragments arranged by size:
3324 1473 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2365 794
Size: 1571 4238
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstYI r'GATC_y
Cuts at: 1972 3139 3506 3891 3908 5360 5446 5458 5804
Size: 1167 367 385 17 1452 86 12 346
Cuts at: 5804 1972
Size: 1977
Fragments arranged by size:
1977 1452 1167 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2615 2615
Size: 5809
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 2624 2742 2808 2972 513
Size: 552 939 414 206 118 66 164 3350
Fragments arranged by size:
3350 939 552 414 206 164 118 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 2087 2927 3325 4119 4139 2087
Size: 840 398 794 20 3757
Fragments arranged by size:
3757 840 794 398 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 3113 3113
Size: 5809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4816 809
Size: 507 3500 1802
Fragments arranged by size:
3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2187 2217
Size: 54 1425 50 275 18 177 180 30
Cuts at: 2217 2322 2369 2381 2522 2627 2739 3256 4145
Size: 105 47 12 141 105 112 517 889
Cuts at: 4145 5774 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 517 275 180 177 141
112 105 105 54 50 47 43 30
18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2187 2381 2522 2627 3256 1537
Size: 275 375 194 141 105 629 4090
Fragments arranged by size:
4090 629 375 275 194 141 105
EarI CTCTTCn'nnn_
Cuts at: 1353 3739 1353
Size: 2386 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2278 3082 4396 4770 4916 387
Size: 1064 138 689 804 1314 374 146 1280
Fragments arranged by size:
1314 1280 1064 804 689 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2978 3021 3872 4469 5256 5524 1239
Size: 393 1346 43 851 597 787 268 1524
Fragments arranged by size:
1524 1346 851 787 597 393 268 43
EcoNI CCTnn'n_nnAGG
Cuts at: 3018 3018
Size: 5809
EcoO109I rG'GnC_Cy
Cuts at: 2255 2506 2514 2533 2619 2640 3010 3011 3023
Size: 251 8 19 86 21 370 1 12
Cuts at: 3023 2255
Size: 5041
Fragments arranged by size:
5041 370 251 86 21 19 12 8
1
EcoRI G'AATT_C
Cuts at: 3118 3496 3118
Size: 378 5431
FspI TGC'GCA
Cuts at: 2126 4315 2126
Size: 2189 3620
HaeII r_GCGC'y
Cuts at: 1678 2577 2738 2795 2890 4956 1678
Size: 899 161 57 95 2066 2531
Fragments arranged by size:
2531 2066 899 161 95 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4450 4482 4524 4556 1373
Size: 32 135 32 2878 32 42 32 2626
Fragments arranged by size:
2878 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3153 3399 669
Size: 988 281 1215 246 3079
Fragments arranged by size:
3079 1215 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5809
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KasI G'GCGC_C
Cuts at: 2573 2791 2573
Size: 218 5591
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4923 5107 1305
Size: 3618 184 2007
Fragments arranged by size:
3618 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3810
Fragments arranged by size:
3810 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2591 3767 4126 4285 518
Size: 648 1242 183 1176 359 159 2042
Fragments arranged by size:
2042 1242 1176 648 359 183 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2206 2365 2420 2810 2974 3225 3902
Size: 463 137 159 55 390 164 251 677
Cuts at: 3902 5050 5295 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 463 390 251 245 164
159 137 55
NaeI GCC'GGC
Cuts at: 2304 2631 2304
Size: 327 5482
NarI GG'CG_CC
Cuts at: 2574 2792 2574
Size: 218 5591
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4318
Fragments arranged by size:
4318 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NgoMIV G'CCGG_C
Cuts at: 2302 2629 2302
Size: 327 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 2187 2381 3256 2187
Size: 194 875 4740
Fragments arranged by size:
4740 875 194
NspI r_CATG'y
Cuts at: 1132 1229 3167 4712 1132
Size: 97 1938 1545 2229
Fragments arranged by size:
2229 1938 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5809
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2640 2640
Size: 5809
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PspOMI G'GGCC_C
Cuts at: 2254 2513 2532 3010 2254
Size: 259 19 478 5053
Fragments arranged by size:
5053 478 259 19
PstI C_TGCA'G
Cuts at: 830 2886 3161 830
Size: 2056 275 3478
Fragments arranged by size:
3478 2056 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
PvuII CAG'CTG
Cuts at: 2069 2069
Size: 5809
SacII CC_GC'GG
Cuts at: 2421 2811 2975 2421
Size: 390 164 5255
Fragments arranged by size:
5255 390 164
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SanDI GG'GwC_CC
Cuts at: 2640 2640
Size: 5809
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 4057 5769 4057
Size: 1712 4097
SfcI C'TryA_G
Cuts at: 826 1045 2882 3157 4292 4973 5164 826
Size: 219 1837 275 1135 681 191 1471
Fragments arranged by size:
1837 1471 1135 681 275 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2521 2540 2626 2521
Size: 19 86 5704
Fragments arranged by size:
5704 86 19
SfoI GGC'GCC
Cuts at: 2575 2793 2575
Size: 218 5591
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 2233 2710 3136 1872
Size: 361 477 426 4545
Fragments arranged by size:
4545 477 426 361
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3913 4814 5076 820
Size: 197 926 1970 901 262 1553
Fragments arranged by size:
1970 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SrfI GCCC'GGGC
Cuts at: 2233 2233
Size: 5809
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5809
StyI C'CwwG_G
Cuts at: 513 1065 2004 3248 513
Size: 552 939 1244 3074
Fragments arranged by size:
3074 1244 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2482 3836 3853 4006 4191 4636 325
Size: 1255 902 1354 17 153 185 445 1498
Fragments arranged by size:
1498 1354 1255 902 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4055 5767 96
Size: 274 80 33 51 1443 2078 1712 138
Fragments arranged by size:
2078 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5769 5801 5769
Size: 32 5777
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2203
Size: 5809
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 2231 2708 3134 1870
Size: 361 477 426 4545
Fragments arranged by size:
4545 477 426 361
XmnI GAAnn'nnTTC
Cuts at: 3938 3938
Size: 5809
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SapI SexAI SwaI