Restriction map for
pFN21AB5263
(Circular) (Six-base) MAPSORT of: pFN21AB5263.seq Check: 5573 from: 1 to: 5842
pFN21AB5263 5842 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2543 2543
Size: 5842
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3652 278
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2358 3185 1937
Size: 421 827 4594
Fragments arranged by size:
4594 827 421
Acc65I G'GTAC_C
Cuts at: 2631 3163 2631
Size: 532 5310
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3104 3905 5289 5557 1632
Size: 1472 801 1384 268 1917
Fragments arranged by size:
1917 1472 1384 801 268
AfeI AGC'GCT
Cuts at: 2976 2976
Size: 5842
AflII C'TTAA_G
Cuts at: 820 1017 2543 820
Size: 197 1526 4119
Fragments arranged by size:
4119 1526 197
AflIII A'CryG_T
Cuts at: 1203 4741 5718 1203
Size: 3538 977 1327
Fragments arranged by size:
3538 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 2689 4571 2689
Size: 1882 3960
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5842
AlwNI CAG_nnn'CTG
Cuts at: 2278 2374 2416 2455 2854 3585 5157 2278
Size: 96 42 39 399 731 1572 2963
Fragments arranged by size:
2963 1572 731 399 96 42 39
ApaI G_GGCC'C
Cuts at: 2123 2641 2123
Size: 518 5324
ApaLI G'TGCA_C
Cuts at: 3899 5055 3899
Size: 1156 4686
ApoI r'AATT_y
Cuts at: 1493 3151 3377 3529 5638 1493
Size: 1658 226 152 2109 1697
Fragments arranged by size:
2109 1697 1658 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2077 3167 1870
Size: 73 125 9 1090 4545
Fragments arranged by size:
4545 1090 125 73 9
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2631 3163 4618 618
Size: 325 217 627 844 532 1455 1842
Fragments arranged by size:
1842 1455 844 627 532 325 217
BanII G_rGCy'C
Cuts at: 1391 2046 2123 2641 2792 3039 3093 3161 1391
Size: 655 77 518 151 247 54 68 4072
Fragments arranged by size:
4072 655 518 247 151 77 68 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3734 4944 3734
Size: 1210 4632
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4453 136
Size: 107 122 71 631 605 337 2444 1525
Fragments arranged by size:
2444 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2500 5837 2500
Size: 3337 2505
BlpI GC'TnA_GC
Cuts at: 2417 3259 2417
Size: 842 5000
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2641 3903 5059 1790
Size: 333 518 1262 1156 2573
Fragments arranged by size:
2573 1262 1156 518 333
BmgBI CAC'GTC
Cuts at: 1318 2116 1318
Size: 798 5044
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3573 4531 442
Size: 416 53 83 399 24 2156 958 1753
Fragments arranged by size:
2156 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3014 3152 4502 1239
Size: 1775 138 1350 2579
Fragments arranged by size:
2579 1775 1350 138
Bpu10I CC'TnA_GC
Cuts at: 2022 2583 2022
Size: 561 5281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2450 3967 4832 5130 2450
Size: 1517 865 298 3162
Fragments arranged by size:
3162 1517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4505 882
Size: 617 3006 2219
Fragments arranged by size:
3006 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2191 3633 493
Size: 711 987 1442 2702
Fragments arranged by size:
2702 1442 987 711
BsaBI GATnn'nnATC
Cuts at: 2273 5836 2273
Size: 3563 2279
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2330 3649 4031 4666
Size: 53 83 186 936 797 1319 382 635
Cuts at: 4666 275
Size: 1451
Fragments arranged by size:
1451 1319 936 797 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2928 4275 4673 4947 5094 5505 2928
Size: 1347 398 274 147 411 3265
Fragments arranged by size:
3265 1347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 1178
Size: 30 165 30 1059 30 4528
Fragments arranged by size:
4528 1059 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3047 1186
Size: 1861 3981
BseYI C'CCAG_C
Cuts at: 5045 5541 5045
Size: 496 5346
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 1163
Size: 573 651 4618
Fragments arranged by size:
4618 651 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 4053 5081 1540
Size: 275 186 1291 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 3093 3903 3988 5059 2493
Size: 600 810 85 1071 3276
Fragments arranged by size:
3276 1071 810 600 85
BsiWI C'GTAC_G
Cuts at: 2160 2160
Size: 5842
BsmI GAATG_Cn'
Cuts at: 2703 3353 3446 2703
Size: 650 93 5099
Fragments arranged by size:
5099 650 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2123 2493 2641 2792 3039 3093
Size: 399 256 77 370 148 151 247 54
Cuts at: 3093 3161 3903 3988 5059 1391
Size: 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 256 247 151
148 85 77 68 54
BspEI T'CCGG_A
Cuts at: 5505 5505
Size: 5842
BspHI T'CATG_A
Cuts at: 3731 5461 3731
Size: 1730 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2526 2543 2928 3197 844
Size: 1682 17 385 269 3489
Fragments arranged by size:
3489 1682 385 269 17
BsrBI CCG'CTC
Cuts at: 1394 1823 3079 3729 1394
Size: 429 1256 650 3507
Fragments arranged by size:
3507 1256 650 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2428 4337 4511 65
Size: 1183 1180 1909 174 1396
Fragments arranged by size:
1909 1396 1183 1180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2597 2727 4486 4673 1700
Size: 110 787 130 1759 187 2869
Fragments arranged by size:
2869 1759 787 187 130 110
BsrGI T'GTAC_A
Cuts at: 96 2391 96
Size: 2295 3547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5842
BstBI TT'CG_AA
Cuts at: 2232 4646 2232
Size: 2414 3428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5842
BstXI CCAn_nnnn'nTGG
Cuts at: 3058 3058
Size: 5842
BstYI r'GATC_y
Cuts at: 1972 2268 2274 2451 2500 3172 3539 3924 3941
Size: 296 6 177 49 672 367 385 17
Cuts at: 3941 5393 5479 5491 5837 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 672 385 367 346 296 177
86 49 17 12 6
Bsu36I CC'TnA_GG
Cuts at: 3072 3072
Size: 5842
BtgI C'CryG_G
Cuts at: 513 1065 2004 3051 513
Size: 552 939 1047 3304
Fragments arranged by size:
3304 1047 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5103
Fragments arranged by size:
5103 683 56
BtsI GCAGTG_nn'
Cuts at: 3358 4152 4172 3358
Size: 794 20 5028
Fragments arranged by size:
5028 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 3146 3146
Size: 5842
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 3027 3289
Size: 54 1425 50 275 18 378 819 262
Cuts at: 3289 4178 5807 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 819 378 275 262 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3289 1537
Size: 275 1477 4090
Fragments arranged by size:
4090 1477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3772 1353
Size: 2419 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 2470 4429 4803 4949 387
Size: 1064 138 690 191 1959 374 146 1280
Fragments arranged by size:
1959 1280 1064 690 374 191 146 138
EcoICRI GAG'CTC
Cuts at: 3091 3091
Size: 5842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3014 3104 3152 3905 4502 5289 5557
Size: 393 1382 90 48 753 597 787 268
Cuts at: 5557 1239
Size: 1524
Fragments arranged by size:
1524 1382 787 753 597 393 268 90
48
EcoNI CCTnn'n_nnAGG
Cuts at: 3071 3071
Size: 5842
EcoO109I rG'GnC_Cy
Cuts at: 2074 2371 2461 2638 2806 3136 2074
Size: 297 90 177 168 330 4780
Fragments arranged by size:
4780 330 297 177 168 90
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
FspI TGC'GCA
Cuts at: 2195 4348 2195
Size: 2153 3689
HaeII r_GCGC'y
Cuts at: 1678 2978 4989 1678
Size: 1300 2011 2531
Fragments arranged by size:
2531 2011 1300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2563 2595 2675 2707 4483
Size: 32 135 32 991 32 80 32 1776
Cuts at: 4483 4515 4557 4589 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1776 991 135 80 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2937 3186 3432 669
Size: 988 281 999 249 246 3079
Fragments arranged by size:
3079 999 988 281 249 246
HindIII A'AGCT_T
Cuts at: 748 2885 748
Size: 2137 3705
HpaI GTT'AAC
Cuts at: 2937 3432 2937
Size: 495 5347
KpnI G_GTAC'C
Cuts at: 2635 3167 2635
Size: 532 5310
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2538 4956 5140 1305
Size: 1233 2418 184 2007
Fragments arranged by size:
2418 2007 1233 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 10
Size: 54 1425 343 378 3642
Fragments arranged by size:
3642 1425 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 2825 3800 4159 4318 518
Size: 648 1132 527 975 359 159 2042
Fragments arranged by size:
2042 1132 975 648 527 359 159
MspA1I CmG'CkG
Cuts at: 1606 2416 3258 3935 5083 5328 1606
Size: 810 842 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 810 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3051 513
Size: 552 939 1047 3304
Fragments arranged by size:
3304 1047 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NspI r_CATG'y
Cuts at: 1132 1229 3200 4745 1132
Size: 97 1971 1545 2229
Fragments arranged by size:
2229 1971 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 1108
Size: 1668 4174
PfoI T'CCnGG_A
Cuts at: 2262 3099 2262
Size: 837 5005
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PmlI CAC'GTG
Cuts at: 2191 2191
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2074 2371 2806 2074
Size: 297 435 5110
Fragments arranged by size:
5110 435 297
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PspOMI G'GGCC_C
Cuts at: 2119 2637 2119
Size: 518 5324
PstI C_TGCA'G
Cuts at: 830 3194 830
Size: 2364 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
PvuII CAG'CTG
Cuts at: 2416 2416
Size: 5842
SacI G_AGCT'C
Cuts at: 3093 3093
Size: 5842
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SanDI GG'GwC_CC
Cuts at: 2074 2074
Size: 5842
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 3002 4090 5802 3002
Size: 1088 1712 3042
Fragments arranged by size:
3042 1712 1088
SfcI C'TryA_G
Cuts at: 826 1045 2846 3190 4325 5006 5197 826
Size: 219 1801 344 1135 681 191 1471
Fragments arranged by size:
1801 1471 1135 681 344 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 2070 2079 3169 1872
Size: 198 9 1090 4545
Fragments arranged by size:
4545 1090 198 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 2543 3946 4847 5109 820
Size: 197 926 522 78 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 522 262 197 78
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5842
StyI C'CwwG_G
Cuts at: 513 1065 2004 2661 2868 3051 3281 513
Size: 552 939 657 207 183 230 3074
Fragments arranged by size:
3074 939 657 552 230 207 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3869 3886 4039 4224 4669 325
Size: 1255 2289 17 153 185 445 1498
Fragments arranged by size:
2289 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 2391 2469
Size: 274 80 33 51 1443 165 249 78
Cuts at: 2469 3000 4088 5800 96
Size: 531 1088 1712 138
Fragments arranged by size:
1712 1443 1088 531 274 249 165 138
80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5802 5834 5802
Size: 32 5810
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2869 2869
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 2068 2077 3167 1870
Size: 198 9 1090 4545
Fragments arranged by size:
4545 1090 198 9
XmnI GAAnn'nnTTC
Cuts at: 3971 3971
Size: 5842
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3650 276
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI