Restriction map for
pFN21AB5260
(Circular) (Six-base) MAPSORT of: pFN21AB5260.seq Check: 1392 from: 1 to: 5848
pFN21AB5260 5848 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3191 1937
Size: 1254 4594
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 3911 5295 5563 1632
Size: 437 1842 1384 268 1917
Fragments arranged by size:
1917 1842 1384 437 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 4747 5724 1203
Size: 3544 977 1327
Fragments arranged by size:
3544 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 2335 4577 2335
Size: 2242 3606
AleI CACnn'nnGTG
Cuts at: 1166 3020 1166
Size: 1854 3994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5848
AlwNI CAG_nnn'CTG
Cuts at: 3591 5163 3591
Size: 1572 4276
ApaI G_GGCC'C
Cuts at: 3036 3087 3036
Size: 51 5797
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 3157 3383 3535 5644 1493
Size: 1664 226 152 2109 1697
Fragments arranged by size:
2109 1697 1664 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3006 3027 3173 1870
Size: 73 1063 21 146 4545
Fragments arranged by size:
4545 1063 146 73 21
BamHI G'GATC_C
Cuts at: 2082 3178 3545 2082
Size: 1096 367 4385
Fragments arranged by size:
4385 1096 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2627 2897 2952 3169 4624
Size: 325 217 627 840 270 55 217 1455
Cuts at: 4624 618
Size: 1842
Fragments arranged by size:
1842 1455 840 627 325 270 217 217
55
BanII G_rGCy'C
Cuts at: 1391 2823 3036 3087 3167 1391
Size: 1432 213 51 80 4072
Fragments arranged by size:
4072 1432 213 80 51
BbeI G_GCGC'C
Cuts at: 2956 2956
Size: 5848
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BbvCI CC'TCA_GC
Cuts at: 2723 2753 2723
Size: 30 5818
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 2536 1548
Size: 988 4860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 4459 136
Size: 107 122 71 631 605 380 2407 1525
Fragments arranged by size:
2407 1525 631 605 380 122 107 71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2900 3036 3087 3909 5065 1790
Size: 1110 136 51 822 1156 2573
Fragments arranged by size:
2573 1156 1110 822 136 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3579 4537 442
Size: 416 53 83 399 24 2162 958 1753
Fragments arranged by size:
2162 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2801 2837 4508 1239
Size: 956 606 36 1671 2579
Fragments arranged by size:
2579 1671 956 606 36
Bpu10I CC'TnA_GC
Cuts at: 2723 2753 2723
Size: 30 5818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 3023 3086 3973 4838 5136 2651
Size: 372 63 887 865 298 3363
Fragments arranged by size:
3363 887 865 372 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2767 4511 882
Size: 617 1268 1744 2219
Fragments arranged by size:
2219 1744 1268 617
BsaAI yAC'GTr
Cuts at: 493 1204 2068 2560 2941 3639 493
Size: 711 864 492 381 698 2702
Fragments arranged by size:
2702 864 711 698 492 381
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2331 2953 3655 4037
Size: 53 83 186 936 798 622 702 382
Cuts at: 4037 4672 275
Size: 635 1451
Fragments arranged by size:
1451 936 798 702 635 622 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2559 2589 1178
Size: 30 165 30 1156 30 4437
Fragments arranged by size:
4437 1156 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2258 2310 2339 2455 2759 2948 1186
Size: 862 210 52 29 116 304 189 4086
Fragments arranged by size:
4086 862 304 210 189 116 52 29
BseYI C'CCAG_C
Cuts at: 2483 3079 5051 5547 2483
Size: 596 1972 496 2784
Fragments arranged by size:
2784 1972 596 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 2090 1163
Size: 573 324 30 4921
Fragments arranged by size:
4921 573 324 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2646 2686 3298 4059 5087 1540
Size: 275 186 645 40 612 761 1028 2301
Fragments arranged by size:
2301 1028 761 645 612 275 186 40
BsiHKAI G_wGCw'C
Cuts at: 2163 2415 3909 3994 5065 2163
Size: 252 1494 85 1071 2946
Fragments arranged by size:
2946 1494 1071 252 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2415 2823 2900 3036 3087 3167
Size: 399 373 252 408 77 136 51 80
Cuts at: 3167 3909 3994 5065 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 373 252 136
85 80 77 51
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2080 3203 844
Size: 1236 1123 3489
Fragments arranged by size:
3489 1236 1123
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2289 2697 2919 3000 3057 4492 4679
Size: 110 479 408 222 81 57 1435 187
Cuts at: 4679 1700
Size: 2869
Fragments arranged by size:
2869 1435 479 408 222 187 110 81
57
BsrGI T'GTAC_A
Cuts at: 96 2316 96
Size: 2220 3628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 2575 1184
Size: 1391 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 3022 3022
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 2082 3096 3178 3545 3930 3947 5399 5485
Size: 110 1014 82 367 385 17 1452 86
Cuts at: 5485 5497 5843 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1014 385 367 346 110 86
82 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2802 513
Size: 552 939 798 3559
Fragments arranged by size:
3559 939 798 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2464 487
Size: 683 56 1238 3871
Fragments arranged by size:
3871 1238 683 56
BtsI GCAGTG_nn'
Cuts at: 2987 3364 4158 4178 2987
Size: 377 794 20 4657
Fragments arranged by size:
4657 794 377 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2108 4855 809
Size: 507 792 2747 1802
Fragments arranged by size:
2747 1802 792 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2388 3012 3295
Size: 54 1425 50 275 18 558 624 283
Cuts at: 3295 4184 5813 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 624 558 283 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2420 3778 1353
Size: 1067 1358 3423
Fragments arranged by size:
3423 1358 1067
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2822 4435 4809 4955 387
Size: 1064 138 1233 1613 374 146 1280
Fragments arranged by size:
1613 1280 1233 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2195 2801 2837 3911 4508 5295
Size: 393 437 126 606 36 1074 597 787
Cuts at: 5295 5563 1239
Size: 268 1524
Fragments arranged by size:
1524 1074 787 606 597 437 393 268
126 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2344 2756 2344
Size: 412 5436
EcoO109I rG'GnC_Cy
Cuts at: 2092 2960 3032 3033 3083 3084 2092
Size: 868 72 1 50 1 4856
Fragments arranged by size:
4856 868 72 50 1 1
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2618 2920 2956 4995 1678
Size: 940 302 36 2039 2531
Fragments arranged by size:
2531 2039 940 302 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2321 2353 4489
Size: 32 135 32 662 32 55 32 2136
Cuts at: 4489 4521 4563 4595 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2136 662 135 55 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KasI G'GCGC_C
Cuts at: 2952 2952
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 4962 5146 1305
Size: 903 2754 184 2007
Fragments arranged by size:
2754 2007 903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2390 10
Size: 54 1425 343 558 3468
Fragments arranged by size:
3468 1425 558 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2427 2708 3020 3806 4165 4324 518
Size: 648 1261 281 312 786 359 159 2042
Fragments arranged by size:
2042 1261 786 648 359 312 281 159
MspA1I CmG'CkG
Cuts at: 1606 2074 3264 3941 5089 5334 1606
Size: 468 1190 677 1148 245 2120
Fragments arranged by size:
2120 1190 1148 677 468 245
NaeI GCC'GGC
Cuts at: 2291 2291
Size: 5848
NarI GG'CG_CC
Cuts at: 2953 2953
Size: 5848
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4357
Fragments arranged by size:
4357 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NgoMIV G'CCGG_C
Cuts at: 2289 2289
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 3206 4751 1132
Size: 97 1977 1545 2229
Fragments arranged by size:
2229 1977 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2634 1348
Size: 1286 4562
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PfoI T'CCnGG_A
Cuts at: 2154 2154
Size: 5848
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2960 2092
Size: 868 4980
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PspOMI G'GGCC_C
Cuts at: 3032 3083 3032
Size: 51 5797
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 5816
PstI C_TGCA'G
Cuts at: 830 2916 3200 830
Size: 2086 284 3478
Fragments arranged by size:
3478 2086 284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2074 2074
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SanDI GG'GwC_CC
Cuts at: 2960 2960
Size: 5848
SapI GCTCTTCn'nnn_
Cuts at: 2420 2420
Size: 5848
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SfcI C'TryA_G
Cuts at: 826 1045 2912 3196 4331 5012 5203 826
Size: 219 1867 284 1135 681 191 1471
Fragments arranged by size:
1867 1471 1135 681 284 219 191
SfoI GGC'GCC
Cuts at: 2954 2954
Size: 5848
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 2289 1700
Size: 589 5259
SmaI CCC'GGG
Cuts at: 1872 3008 3029 3175 1872
Size: 1136 21 146 4545
Fragments arranged by size:
4545 1136 146 21
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 3038 3101 3952 4853 5115
Size: 197 926 723 372 63 851 901 262
Cuts at: 5115 820
Size: 1553
Fragments arranged by size:
1553 926 901 851 723 372 262 197
63
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2391 3287 513
Size: 552 939 357 30 896 3074
Fragments arranged by size:
3074 939 896 552 357 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2563 3875 3892 4045 4230 4675 325
Size: 1255 983 1312 17 153 185 445 1498
Fragments arranged by size:
1498 1312 1255 983 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2316 4094 5806
Size: 274 80 33 51 1443 339 1778 1712
Cuts at: 5806 96
Size: 138
Fragments arranged by size:
1778 1712 1443 339 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2559 2591 5808 5840 2559
Size: 32 3217 32 2567
Fragments arranged by size:
3217 2567 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2383 2834 2864 2383
Size: 451 30 5367
Fragments arranged by size:
5367 451 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3006 3027 3173 1870
Size: 1136 21 146 4545
Fragments arranged by size:
4545 1136 146 21
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI RsrII
SacI SacII SexAI SfiI SrfI SwaI