Restriction map for
pFN21AB5258
(Circular) (Six-base) MAPSORT of: pFN21AB5258.seq Check: 1552 from: 1 to: 5677
pFN21AB5258 5677 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2617 2695 3020 1937
Size: 680 78 325 4594
Fragments arranged by size:
4594 680 325 78
Acc65I G'GTAC_C
Cuts at: 2844 2998 2844
Size: 154 5523
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3740 5124 5392 1632
Size: 2108 1384 268 1917
Fragments arranged by size:
2108 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 2820 4406 2820
Size: 1586 4091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 2387 3420 4992 2387
Size: 1033 1572 3072
Fragments arranged by size:
3072 1572 1033
ApaI G_GGCC'C
Cuts at: 2750 2750
Size: 5677
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2938 2986 3212 3364 5473 1493
Size: 1445 48 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 226 152 48
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2951 3002 1870
Size: 73 176 832 51 4545
Fragments arranged by size:
4545 832 176 73 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2828 2861 2828
Size: 33 5644
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 2844 2998 4453 618
Size: 325 217 627 353 704 154 1455 1842
Fragments arranged by size:
1842 1455 704 627 353 325 217 154
BanII G_rGCy'C
Cuts at: 1391 2750 2996 1391
Size: 1359 246 4072
Fragments arranged by size:
4072 1359 246
BbeI G_GCGC'C
Cuts at: 2144 2144
Size: 5677
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4706
BbvCI CC'TCA_GC
Cuts at: 2063 2973 2063
Size: 910 4767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 4288 136
Size: 107 122 71 631 605 452 2164 1525
Fragments arranged by size:
2164 1525 631 605 452 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 2958 3094 2958
Size: 136 5541
Bme1580I G_kGCm'C
Cuts at: 1790 2750 3738 4894 1790
Size: 960 988 1156 2573
Fragments arranged by size:
2573 1156 988 960
BmgBI CAC'GTC
Cuts at: 1318 2453 1318
Size: 1135 4542
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3408 4366 442
Size: 416 53 83 399 24 1991 958 1753
Fragments arranged by size:
1991 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4337 1239
Size: 3098 2579
Bpu10I CC'TnA_GC
Cuts at: 2063 2973 2063
Size: 910 4767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 2596 2807 4340 882
Size: 617 516 581 211 1533 2219
Fragments arranged by size:
2219 1533 617 581 516 211
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 2614 2875 3484
Size: 53 83 186 936 608 473 261 609
Cuts at: 3484 3866 4501 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 609 608 473 382 261
186 83 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2313 2343 1178
Size: 30 165 30 910 30 4512
Fragments arranged by size:
4512 910 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2742 1186
Size: 1556 4121
BseYI C'CCAG_C
Cuts at: 2025 4880 5376 2025
Size: 2855 496 2326
Fragments arranged by size:
2855 2326 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2663 2700 1163
Size: 573 927 37 4140
Fragments arranged by size:
4140 927 573 37
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2536 3738 3823 4894 2536
Size: 1202 85 1071 3319
Fragments arranged by size:
3319 1202 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2536 2750 2996 3738 3823 4894 1391
Size: 399 746 214 246 742 85 1071 2174
Fragments arranged by size:
2174 1071 746 742 399 246 214 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2112 2948 3564 1394
Size: 429 289 836 616 3507
Fragments arranged by size:
3507 836 616 429 289
BsrDI GCAATG_nn'
Cuts at: 65 1248 2788 4172 4346 65
Size: 1183 1540 1384 174 1396
Fragments arranged by size:
1540 1396 1384 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 2559 4321 4508 1700
Size: 110 337 412 1762 187 2869
Fragments arranged by size:
2869 1762 412 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 2260 96
Size: 2164 3513
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2152 487
Size: 683 56 926 4012
Fragments arranged by size:
4012 926 683 56
BtsI GCAGTG_nn'
Cuts at: 2314 3193 3987 4007 2314
Size: 879 794 20 3984
Fragments arranged by size:
3984 879 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2162 4684 809
Size: 507 846 2522 1802
Fragments arranged by size:
2522 1802 846 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642
Size: 54 1425 50 275 18 1294 889 1629
Cuts at: 5642 8
Size: 43
Fragments arranged by size:
1629 1425 1294 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3607 1353
Size: 2254 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3740 4337 5124 5392 1239
Size: 393 2108 597 787 268 1524
Fragments arranged by size:
2108 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2569 2569
Size: 5677
EcoO109I rG'GnC_Cy
Cuts at: 2384 2462 2746 2747 2384
Size: 78 284 1 5314
Fragments arranged by size:
5314 284 78 1
EcoRI G'AATT_C
Cuts at: 2938 2986 3364 2938
Size: 48 378 5251
Fragments arranged by size:
5251 378 48
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2070 2144 4824 1678
Size: 392 74 2680 2531
Fragments arranged by size:
2680 2531 392 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2806 2838 2927 2959 4318
Size: 32 135 32 1234 32 89 32 1359
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1359 1234 135 89 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2618 3021 3267 669
Size: 988 281 680 403 246 3079
Fragments arranged by size:
3079 988 680 403 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KasI G'GCGC_C
Cuts at: 2140 2140
Size: 5677
KpnI G_GTAC'C
Cuts at: 2848 3002 2848
Size: 154 5523
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 4791 4975 1305
Size: 1350 2136 184 2007
Fragments arranged by size:
2136 2007 1350 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3855
Fragments arranged by size:
3855 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3635 3994 4153 518
Size: 648 2469 359 159 2042
Fragments arranged by size:
2469 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2933 3093 3770 4918 5163 1606
Size: 494 833 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 833 677 494 245 160
NaeI GCC'GGC
Cuts at: 2149 2561 2149
Size: 412 5265
NarI GG'CG_CC
Cuts at: 2141 2141
Size: 5677
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5125
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NgoMIV G'CCGG_C
Cuts at: 2147 2559 2147
Size: 412 5265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2599 3035 4580 1132
Size: 97 1370 436 1545 2229
Fragments arranged by size:
2229 1545 1370 436 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2900 1108
Size: 1792 3885
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2384 2462 2384
Size: 78 5599
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PspOMI G'GGCC_C
Cuts at: 2746 2746
Size: 5677
PstI C_TGCA'G
Cuts at: 830 3029 830
Size: 2199 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2933 2933
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 2616 3019 1936
Size: 680 403 4594
Fragments arranged by size:
4594 680 403
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 3025 4160 4841 5032 826
Size: 219 1980 1135 681 191 1471
Fragments arranged by size:
1980 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2124 2124
Size: 5677
SfoI GGC'GCC
Cuts at: 2142 2142
Size: 5677
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 2121 3004 1872
Size: 249 883 4545
Fragments arranged by size:
4545 883 249
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3781 4682 4944 820
Size: 197 926 1838 901 262 1553
Fragments arranged by size:
1838 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SrfI GCCC'GGGC
Cuts at: 2121 2121
Size: 5677
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2244 2818 3116 513
Size: 552 1179 574 298 3074
Fragments arranged by size:
3074 1179 574 552 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3704 3721 3874 4059 4504 325
Size: 1255 2124 17 153 185 445 1498
Fragments arranged by size:
2124 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2260 3923 5635
Size: 274 80 33 51 1443 283 1663 1712
Cuts at: 5635 96
Size: 138
Fragments arranged by size:
1712 1663 1443 283 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2313 2345 5637 5669 2313
Size: 32 3292 32 2321
Fragments arranged by size:
3292 2321 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 2119 3002 1870
Size: 249 883 4545
Fragments arranged by size:
4545 883 249
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SwaI
XcmI