Restriction map for
pFN21AB5238
(Circular) (Six-base) MAPSORT of: pFN21AB5238.seq Check: 8700 from: 1 to: 5326
pFN21AB5238 5326 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2544 3136 278
Size: 53 83 186 936 1008 592 2468
Fragments arranged by size:
2468 1008 936 592 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 1937
Size: 732 4594
Acc65I G'GTAC_C
Cuts at: 2647 2647
Size: 5326
AclI AA'CG_TT
Cuts at: 1563 3453 3826 1563
Size: 1890 373 3063
Fragments arranged by size:
3063 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2221 2222 2420 3389 4773 5041 1632
Size: 589 1 198 969 1384 268 1917
Fragments arranged by size:
1917 1384 969 589 268 198 1
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5129
AflIII A'CryG_T
Cuts at: 1203 4225 5202 1203
Size: 3022 977 1327
Fragments arranged by size:
3022 1327 977
AgeI A'CCGG_T
Cuts at: 4157 4157
Size: 5326
AhdI GACnn_n'nnGTC
Cuts at: 4055 4055
Size: 5326
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5326
AlwNI CAG_nnn'CTG
Cuts at: 3069 4641 3069
Size: 1572 3754
ApaLI G'TGCA_C
Cuts at: 2248 3383 4539 2248
Size: 1135 1156 3035
Fragments arranged by size:
3035 1156 1135
ApoI r'AATT_y
Cuts at: 1493 2635 2861 3013 5122 1493
Size: 1142 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3880 160
Size: 872 2848 1606
Fragments arranged by size:
2848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
AvaI C'yCGr_G
Cuts at: 1870 1943 2651 1870
Size: 73 708 4545
Fragments arranged by size:
4545 708 73
BamHI G'GATC_C
Cuts at: 2656 3023 2656
Size: 367 4959
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2647 4102 618
Size: 325 217 627 860 1455 1842
Fragments arranged by size:
1842 1455 860 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2105 2245 2645 1391
Size: 714 140 400 4072
Fragments arranged by size:
4072 714 400 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2524 2558 3517 3551 1903
Size: 34 50 34 503 34 959 34 3678
Fragments arranged by size:
3678 959 503 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3218 4428 3218
Size: 1210 4116
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2322 3937
Size: 107 122 71 631 605 337 313 1615
Cuts at: 3937 136
Size: 1525
Fragments arranged by size:
1615 1525 631 605 337 313 122 107
71
BglII A'GATC_T
Cuts at: 5321 5321
Size: 5326
BlpI GC'TnA_GC
Cuts at: 2743 2743
Size: 5326
Bme1580I G_kGCm'C
Cuts at: 1790 2252 3387 4543 1790
Size: 462 1135 1156 2573
Fragments arranged by size:
2573 1156 1135 462
BmgBI CAC'GTC
Cuts at: 1318 2158 2524 1318
Size: 840 366 4120
Fragments arranged by size:
4120 840 366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2455 3057 4015
Size: 416 53 83 399 24 1038 602 958
Cuts at: 4015 442
Size: 1753
Fragments arranged by size:
1753 1038 958 602 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 3986 1239
Size: 977 1770 2579
Fragments arranged by size:
2579 1770 977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3451 4316 4614 3451
Size: 865 298 4163
Fragments arranged by size:
4163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3989 882
Size: 617 2490 2219
Fragments arranged by size:
2490 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2407 3117 493
Size: 711 1203 710 2702
Fragments arranged by size:
2702 1203 711 710
BsaBI GATnn'nnATC
Cuts at: 2600 5320 2600
Size: 2720 2606
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 2541 3133 3515
Size: 53 83 186 936 630 378 592 382
Cuts at: 3515 4150 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 630 592 382 378 186
83 53
BsaWI w'CCGG_w
Cuts at: 3759 4157 4431 4578 4989 3759
Size: 398 274 147 411 4096
Fragments arranged by size:
4096 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 1178
Size: 30 165 30 861 30 4210
Fragments arranged by size:
4210 861 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2116 2119 1186
Size: 930 3 4393
Fragments arranged by size:
4393 930 3
BseYI C'CCAG_C
Cuts at: 4529 5025 4529
Size: 496 4830
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2458 1163
Size: 573 722 4031
Fragments arranged by size:
4031 722 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2185 2776 3537 4565 1540
Size: 275 186 184 591 761 1028 2301
Fragments arranged by size:
2301 1028 761 591 275 186 184
BsiHKAI G_wGCw'C
Cuts at: 2252 3387 3472 4543 2252
Size: 1135 85 1071 3035
Fragments arranged by size:
3035 1135 1071 85
BsmI GAATG_Cn'
Cuts at: 2837 2930 2837
Size: 93 5233
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2245 2252 2645 3387 3472 4543
Size: 399 315 140 7 393 742 85 1071
Cuts at: 4543 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 393 315 140 85
7
BspEI T'CCGG_A
Cuts at: 4989 4989
Size: 5326
BspHI T'CATG_A
Cuts at: 3215 4945 3215
Size: 1730 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2681 844
Size: 1837 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2143 2179 3213 1394
Size: 429 320 36 1034 3507
Fragments arranged by size:
3507 1034 429 320 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 3821 3995 65
Size: 1183 2573 174 1396
Fragments arranged by size:
2573 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3970 4157 1700
Size: 110 2160 187 2869
Fragments arranged by size:
2869 2160 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5326
BssSI C'ACGA_G
Cuts at: 2144 3386 4398 2144
Size: 1242 1012 3072
Fragments arranged by size:
3072 1242 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5326
BstBI TT'CG_AA
Cuts at: 4130 4130
Size: 5326
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5326
BstYI r'GATC_y
Cuts at: 1972 2656 3023 3408 3425 4877 4963 4975 5321
Size: 684 367 385 17 1452 86 12 346
Cuts at: 5321 1972
Size: 1977
Fragments arranged by size:
1977 1452 684 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2079 2427 513
Size: 552 1014 348 3412
Fragments arranged by size:
3412 1014 552 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4587
Fragments arranged by size:
4587 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3636 3656 2842
Size: 794 20 4512
Fragments arranged by size:
4512 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5291
DraI TTT'AAA
Cuts at: 2630 2630
Size: 5326
DraIII CAC_nnn'GTG
Cuts at: 2296 2296
Size: 5326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4333 809
Size: 507 3017 1802
Fragments arranged by size:
3017 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 2614 2773
Size: 54 1425 50 275 18 352 432 159
Cuts at: 2773 3662 5291 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 432 352 275 159 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2182 2773 1537
Size: 275 370 591 4090
Fragments arranged by size:
4090 591 370 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2125 3256 1353
Size: 772 1131 3423
Fragments arranged by size:
3423 1131 772
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3913 4287 4433 387
Size: 1064 138 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2221 2222 2420 3389 3986 4773
Size: 393 584 5 1 198 969 597 787
Cuts at: 4773 5041 1239
Size: 268 1524
Fragments arranged by size:
1524 969 787 597 584 393 268 198
5 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2290 2290
Size: 5326
EcoRI G'AATT_C
Cuts at: 2635 3013 2635
Size: 378 4948
FspI TGC'GCA
Cuts at: 3832 3832
Size: 5326
HaeII r_GCGC'y
Cuts at: 1678 2015 4473 1678
Size: 337 2458 2531
Fragments arranged by size:
2531 2458 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 3967 3999 4041
Size: 32 135 32 530 32 1833 32 42
Cuts at: 4041 4073 1373
Size: 32 2626
Fragments arranged by size:
2626 1833 530 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 2916 669
Size: 988 281 732 246 3079
Fragments arranged by size:
3079 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5326
HpaI GTT'AAC
Cuts at: 2916 2916
Size: 5326
KpnI G_GTAC'C
Cuts at: 2651 2651
Size: 5326
MfeI C'AATT_G
Cuts at: 2925 2925
Size: 5326
MluI A'CGCG_T
Cuts at: 5202 5202
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2475 4440 4624 1305
Size: 1170 1965 184 2007
Fragments arranged by size:
2007 1965 1170 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2616 10
Size: 54 1425 343 784 2720
Fragments arranged by size:
2720 1425 784 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3284 3643 3802 518
Size: 648 2118 359 159 2042
Fragments arranged by size:
2118 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2031 2068 2081 2228 2742 3419 4567 4812
Size: 425 37 13 147 514 677 1148 245
Cuts at: 4812 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 514 425 245 147 37
13
NcoI C'CATG_G
Cuts at: 513 1065 2427 513
Size: 552 1362 3412
Fragments arranged by size:
3412 1362 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5326
NotI GC'GGCC_GC
Cuts at: 2773 2773
Size: 5326
NspI r_CATG'y
Cuts at: 1132 1229 2510 2684 4229 1132
Size: 97 1281 174 1545 2229
Fragments arranged by size:
2229 1545 1281 174 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5326
PciI A'CATG_T
Cuts at: 4225 4225
Size: 5326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5326
PfoI T'CCnGG_A
Cuts at: 2132 2132
Size: 5326
PmeI GTTT'AAAC
Cuts at: 2630 2630
Size: 5326
PmlI CAC'GTG
Cuts at: 2407 2407
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 4908 4940 3395
Size: 32 1481 32 3781
Fragments arranged by size:
3781 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5326
PsiI TTA'TAA
Cuts at: 2896 2896
Size: 5326
PstI C_TGCA'G
Cuts at: 830 2096 2678 830
Size: 1266 582 3478
Fragments arranged by size:
3478 1266 582
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5326
PvuII CAG'CTG
Cuts at: 2228 2228
Size: 5326
SacII CC_GC'GG
Cuts at: 2082 2082
Size: 5326
SalI G'TCGA_C
Cuts at: 1936 2668 1936
Size: 732 4594
SbfI CC_TGCA'GG
Cuts at: 2678 2678
Size: 5326
ScaI AGT'ACT
Cuts at: 3574 5286 3574
Size: 1712 3614
SfcI C'TryA_G
Cuts at: 826 1045 2092 2674 3809 4490 4681 826
Size: 219 1047 582 1135 681 191 1471
Fragments arranged by size:
1471 1135 1047 681 582 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5326
SmaI CCC'GGG
Cuts at: 1872 2653 1872
Size: 781 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3430 4331 4593 820
Size: 197 926 1487 901 262 1553
Fragments arranged by size:
1553 1487 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3117 493
Size: 2624 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5326
SphI G_CATG'C
Cuts at: 1132 2684 1132
Size: 1552 3774
SspI AAT'ATT
Cuts at: 5 52 3250 5
Size: 47 3198 2081
Fragments arranged by size:
3198 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5326
StyI C'CwwG_G
Cuts at: 513 1065 2427 2765 513
Size: 552 1362 338 3074
Fragments arranged by size:
3074 1362 552 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2507 3353 3370 3523 3708 4153 325
Size: 1255 927 846 17 153 185 445 1498
Fragments arranged by size:
1498 1255 927 846 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3572 5284 96
Size: 274 80 33 51 1443 1595 1712 138
Fragments arranged by size:
1712 1595 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5286 5318 5286
Size: 32 5294
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5326
XbaI T'CTAG_A
Cuts at: 2662 2662
Size: 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5326
XmaI C'CCGG_G
Cuts at: 1870 2651 1870
Size: 781 4545
XmnI GAAnn'nnTTC
Cuts at: 2264 3455 2264
Size: 1191 4135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2542 3134 276
Size: 53 83 186 936 1008 592 2468
Fragments arranged by size:
2468 1008 936 592 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI