Restriction map for
pFN21AB5234
(Circular) (Six-base) MAPSORT of: pFN21AB5234.seq Check: 7466 from: 1 to: 6307
pFN21AB5234 6307 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2471 3650 1937
Size: 534 1179 4594
Fragments arranged by size:
4594 1179 534
Acc65I G'GTAC_C
Cuts at: 2865 3628 2865
Size: 763 5544
AclI AA'CG_TT
Cuts at: 1563 4434 4807 1563
Size: 2871 373 3063
Fragments arranged by size:
3063 2871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 4370 5754 6022 1632
Size: 876 1862 1384 268 1917
Fragments arranged by size:
1917 1862 1384 876 268
AfeI AGC'GCT
Cuts at: 2543 3068 2543
Size: 525 5782
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6110
AflIII A'CryG_T
Cuts at: 1203 3468 5206 6183 1203
Size: 2265 1738 977 1327
Fragments arranged by size:
2265 1738 1327 977
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 3520 5036 3520
Size: 1516 4791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2711 1184
Size: 1527 4780
AlwNI CAG_nnn'CTG
Cuts at: 2183 2432 3139 3280 4050 5622 2183
Size: 249 707 141 770 1572 2868
Fragments arranged by size:
2868 1572 770 707 249 141
ApaI G_GGCC'C
Cuts at: 2296 2611 2296
Size: 315 5992
ApaLI G'TGCA_C
Cuts at: 2987 4364 5520 2987
Size: 1377 1156 3774
Fragments arranged by size:
3774 1377 1156
ApoI r'AATT_y
Cuts at: 1493 3616 3842 3994 6103 1493
Size: 2123 226 152 2109 1697
Fragments arranged by size:
2123 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4861 160
Size: 872 3829 1606
Fragments arranged by size:
3829 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 2385 2649 3411 3632 1870
Size: 73 442 264 762 221 4545
Fragments arranged by size:
4545 762 442 264 221 73
BamHI G'GATC_C
Cuts at: 3637 4004 3637
Size: 367 5940
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2865 3628 5083 618
Size: 325 217 627 241 837 763 1455 1842
Fragments arranged by size:
1842 1455 837 763 627 325 241 217
BanII G_rGCy'C
Cuts at: 1391 2021 2296 2418 2611 3420 3626 1391
Size: 630 275 122 193 809 206 4072
Fragments arranged by size:
4072 809 630 275 206 193 122
BbeI G_GCGC'C
Cuts at: 2032 2032
Size: 6307
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5336
BbvCI CC'TCA_GC
Cuts at: 2955 2955
Size: 6307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2266 2300 4498 4532 1903
Size: 34 329 34 2198 34 3678
Fragments arranged by size:
3678 2198 329 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4199 5409 4199
Size: 1210 5097
BclI T'GATC_A
Cuts at: 1548 3231 1548
Size: 1683 4624
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4918 136
Size: 107 122 71 631 605 3246 1525
Fragments arranged by size:
3246 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2562 6302 2562
Size: 3740 2567
BlpI GC'TnA_GC
Cuts at: 2184 3724 2184
Size: 1540 4767
Bme1580I G_kGCm'C
Cuts at: 1790 2296 2611 2991 4368 5524 1790
Size: 506 315 380 1377 1156 2573
Fragments arranged by size:
2573 1377 1156 506 380 315
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6307
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4038 4996 442
Size: 416 53 83 399 24 2621 958 1753
Fragments arranged by size:
2621 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3428 1056
Size: 2372 3935
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3510 3510
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2924 4967 1239
Size: 1685 2043 2579
Fragments arranged by size:
2579 2043 1685
Bpu10I CC'TnA_GC
Cuts at: 2955 2955
Size: 6307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3047 3081 4432 5297 5595 3047
Size: 34 1351 865 298 3759
Fragments arranged by size:
3759 1351 865 298 34
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4970 882
Size: 617 3471 2219
Fragments arranged by size:
3471 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2239 4098 493
Size: 711 1035 1859 2702
Fragments arranged by size:
2702 1859 1035 711
BsaBI GATnn'nnATC
Cuts at: 3398 6301 3398
Size: 2903 3404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 4114 4496 5131
Size: 53 83 186 936 496 2085 382 635
Cuts at: 5131 275
Size: 1451
Fragments arranged by size:
2085 1451 936 635 496 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2709 4740 5138 5412 5559 5970 2709
Size: 2031 398 274 147 411 3046
Fragments arranged by size:
3046 2031 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6082
Fragments arranged by size:
6082 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2082 2135 2501 1186
Size: 896 53 366 4992
Fragments arranged by size:
4992 896 366 53
BseYI C'CCAG_C
Cuts at: 2193 3019 3420 5510 6006 2193
Size: 826 401 2090 496 2494
Fragments arranged by size:
2494 2090 826 496 401
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 1163
Size: 573 639 5095
Fragments arranged by size:
5095 639 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3757 4518 5546 1540
Size: 275 186 1756 761 1028 2301
Fragments arranged by size:
2301 1756 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2238 2991 4368 4453 5524 2190
Size: 48 753 1377 85 1071 2973
Fragments arranged by size:
2973 1377 1071 753 85 48
BsmI GAATG_Cn'
Cuts at: 3818 3911 3818
Size: 93 6214
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2190 2238 2296 2418 2611 2991
Size: 399 231 169 48 58 122 193 380
Cuts at: 2991 3420 3626 4368 4453 5524 1391
Size: 429 206 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 380 231 206
193 169 122 85 58 48
BspEI T'CCGG_A
Cuts at: 2709 5970 2709
Size: 3261 3046
BspHI T'CATG_A
Cuts at: 2526 4196 5926 2526
Size: 1670 1730 2907
Fragments arranged by size:
2907 1730 1670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3662 844
Size: 2818 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4194 1394
Size: 429 2371 3507
Fragments arranged by size:
3507 2371 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4802 4976 65
Size: 1183 3554 174 1396
Fragments arranged by size:
3554 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 3036 4951 5138 1700
Size: 110 282 944 1915 187 2869
Fragments arranged by size:
2869 1915 944 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6307
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6307
BssSI C'ACGA_G
Cuts at: 2513 3362 4367 5379 2513
Size: 849 1005 1012 3441
Fragments arranged by size:
3441 1012 1005 849
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6307
BstBI TT'CG_AA
Cuts at: 5111 5111
Size: 6307
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6307
BstXI CCAn_nnnn'nTGG
Cuts at: 2902 3577 2902
Size: 675 5632
BstYI r'GATC_y
Cuts at: 1972 2562 3027 3637 4004 4389 4406 5858 5944
Size: 590 465 610 367 385 17 1452 86
Cuts at: 5944 5956 6302 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 610 590 465 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2032 2418 513
Size: 552 967 386 4402
Fragments arranged by size:
4402 967 552 386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3253 487
Size: 683 56 2027 3541
Fragments arranged by size:
3541 2027 683 56
BtsI GCAGTG_nn'
Cuts at: 3823 4617 4637 3823
Size: 794 20 5493
Fragments arranged by size:
5493 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6272
DraI TTT'AAA
Cuts at: 3611 3611
Size: 6307
DraIII CAC_nnn'GTG
Cuts at: 3208 3208
Size: 6307
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5314 809
Size: 507 3998 1802
Fragments arranged by size:
3998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3754 4643 6272
Size: 54 1425 50 275 18 1924 889 1629
Cuts at: 6272 8
Size: 43
Fragments arranged by size:
1924 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3754 1537
Size: 275 1942 4090
Fragments arranged by size:
4090 1942 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 4237 1353
Size: 1053 1831 3423
Fragments arranged by size:
3423 1831 1053
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 3038 4894 5268 5414 387
Size: 1064 138 490 959 1856 374 146 1280
Fragments arranged by size:
1856 1280 1064 959 490 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 2924 4370 4967 5754 6022 1239
Size: 393 876 416 1446 597 787 268 1524
Fragments arranged by size:
1524 1446 876 787 597 416 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2945 2945
Size: 6307
EcoO109I rG'GnC_Cy
Cuts at: 2293 2608 2950 3136 3174 2293
Size: 315 342 186 38 5426
Fragments arranged by size:
5426 342 315 186 38
EcoRI G'AATT_C
Cuts at: 3616 3994 3616
Size: 378 5929
EcoRV GAT'ATC
Cuts at: 3460 3460
Size: 6307
FspI TGC'GCA
Cuts at: 2687 4813 2687
Size: 2126 4181
HaeII r_GCGC'y
Cuts at: 1678 2032 2545 3070 5454 1678
Size: 354 513 525 2384 2531
Fragments arranged by size:
2531 2384 525 513 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2578 2610 3506 3538 4948
Size: 32 135 32 1006 32 896 32 1410
Cuts at: 4948 4980 5022 5054 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1410 1006 896 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2145 2472 3651 3897 669
Size: 988 281 207 327 1179 246 3079
Fragments arranged by size:
3079 1179 988 327 281 246 207
HindIII A'AGCT_T
Cuts at: 748 2993 748
Size: 2245 4062
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KasI G'GCGC_C
Cuts at: 2028 2028
Size: 6307
KpnI G_GTAC'C
Cuts at: 2869 3632 2869
Size: 763 5544
MfeI C'AATT_G
Cuts at: 3906 3906
Size: 6307
MluI A'CGCG_T
Cuts at: 6183 6183
Size: 6307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2530 3267 5421 5605 1305
Size: 1225 737 2154 184 2007
Fragments arranged by size:
2154 2007 1225 737 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4485
Fragments arranged by size:
4485 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 3005 4265 4624 4783 518
Size: 648 1359 480 1260 359 159 2042
Fragments arranged by size:
2042 1359 1260 648 480 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2180 2902 2966 3723 4400 5548 5793
Size: 428 146 722 64 757 677 1148 245
Cuts at: 5793 1606
Size: 2120
Fragments arranged by size:
2120 1148 757 722 677 428 245 146
64
NaeI GCC'GGC
Cuts at: 2094 2094
Size: 6307
NarI GG'CG_CC
Cuts at: 2029 2029
Size: 6307
NcoI C'CATG_G
Cuts at: 513 1065 2418 513
Size: 552 1353 4402
Fragments arranged by size:
4402 1353 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6307
NgoMIV G'CCGG_C
Cuts at: 2092 2092
Size: 6307
NheI G'CTAG_C
Cuts at: 1052 3424 1052
Size: 2372 3935
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 2702 3472 3665 5210 1132
Size: 97 1473 770 193 1545 2229
Fragments arranged by size:
2229 1545 1473 770 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6307
PciI A'CATG_T
Cuts at: 3468 5206 3468
Size: 1738 4569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2107 3139 1108
Size: 999 1032 4276
Fragments arranged by size:
4276 1032 999
PfoI T'CCnGG_A
Cuts at: 2406 2664 3138 3330 2406
Size: 258 474 192 5383
Fragments arranged by size:
5383 474 258 192
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PmlI CAC'GTG
Cuts at: 2239 2239
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3136 3174 3136
Size: 38 6269
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6307
PsiI TTA'TAA
Cuts at: 3877 3877
Size: 6307
PspOMI G'GGCC_C
Cuts at: 2292 2607 2292
Size: 315 5992
PstI C_TGCA'G
Cuts at: 830 3045 3285 3529 3659 830
Size: 2215 240 244 130 3478
Fragments arranged by size:
3478 2215 244 240 130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
PvuII CAG'CTG
Cuts at: 2902 2966 2902
Size: 64 6243
SacII CC_GC'GG
Cuts at: 2035 2035
Size: 6307
SalI G'TCGA_C
Cuts at: 1936 2470 3649 1936
Size: 534 1179 4594
Fragments arranged by size:
4594 1179 534
SapI GCTCTTCn'nnn_
Cuts at: 2406 2406
Size: 6307
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 2593 2909 4555 6267 2593
Size: 316 1646 1712 2633
Fragments arranged by size:
2633 1712 1646 316
SexAI A'CCwGG_T
Cuts at: 3132 3132
Size: 6307
SfcI C'TryA_G
Cuts at: 826 1045 2785 3041 3281 3525 3655 4790 5471
Size: 219 1740 256 240 244 130 1135 681
Cuts at: 5471 5662 826
Size: 191 1471
Fragments arranged by size:
1740 1471 1135 681 256 244 240 219
191 130
SfoI GGC'GCC
Cuts at: 2030 2030
Size: 6307
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6307
SmaI CCC'GGG
Cuts at: 1872 2651 3634 1872
Size: 779 983 4545
Fragments arranged by size:
4545 983 779
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2385 3062 3096 4411 5312 5574
Size: 197 926 442 677 34 1315 901 262
Cuts at: 5574 820
Size: 1553
Fragments arranged by size:
1553 1315 926 901 677 442 262 197
34
SnaBI TAC'GTA
Cuts at: 493 4098 493
Size: 3605 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6307
SphI G_CATG'C
Cuts at: 1132 2702 3665 1132
Size: 1570 963 3774
Fragments arranged by size:
3774 1570 963
SspI AAT'ATT
Cuts at: 5 52 4231 5
Size: 47 4179 2081
Fragments arranged by size:
4179 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6307
StyI C'CwwG_G
Cuts at: 513 1065 2217 2418 3258 3321 3384 3570 3746
Size: 552 1152 201 840 63 63 186 176
Cuts at: 3746 513
Size: 3074
Fragments arranged by size:
3074 1152 840 552 201 186 176 63
63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4334 4351 4504 4689 5134 325
Size: 1255 2754 17 153 185 445 1498
Fragments arranged by size:
2754 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2591 2907 4553
Size: 274 80 33 51 1443 614 316 1646
Cuts at: 4553 6265 96
Size: 1712 138
Fragments arranged by size:
1712 1646 1443 614 316 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6267 6299 6267
Size: 32 6275
Tth111I GACn'n_nGTC
Cuts at: 1316 2210 1316
Size: 894 5413
XbaI T'CTAG_A
Cuts at: 3643 3643
Size: 6307
XhoI C'TCGA_G
Cuts at: 1943 2385 1943
Size: 442 5865
XmaI C'CCGG_G
Cuts at: 1870 2649 3632 1870
Size: 779 983 4545
Fragments arranged by size:
4545 983 779
XmnI GAAnn'nnTTC
Cuts at: 4436 4436
Size: 6307
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI FalI FseI FspAI NruI NsiI
PacI PspXI PsrI RsrII SacI SanDI SfiI SrfI
SwaI XcmI