Restriction map for
pFN21AB5232
(Circular) (Six-base) MAPSORT of: pFN21AB5232.seq Check: 8311 from: 1 to: 6211
pFN21AB5232 6211 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3195 3195
Size: 6211
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3554 1937
Size: 1617 4594
Acc65I G'GTAC_C
Cuts at: 3180 3197 3532 3180
Size: 17 335 5859
Fragments arranged by size:
5859 335 17
AclI AA'CG_TT
Cuts at: 1563 3141 4338 4711 1563
Size: 1578 1197 373 3063
Fragments arranged by size:
3063 1578 1197 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2526 2660 2731 3400 3467 4274 5658 5926
Size: 894 134 71 669 67 807 1384 268
Cuts at: 5926 1632
Size: 1917
Fragments arranged by size:
1917 1384 894 807 669 268 134 71
67
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 5110 6087 1203
Size: 3907 977 1327
Fragments arranged by size:
3907 1327 977
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6211
AlwNI CAG_nnn'CTG
Cuts at: 2635 2638 2754 3954 5526 2635
Size: 3 116 1200 1572 3320
Fragments arranged by size:
3320 1572 1200 116 3
ApaI G_GGCC'C
Cuts at: 2145 2145
Size: 6211
ApaLI G'TGCA_C
Cuts at: 4268 5424 4268
Size: 1156 5055
ApoI r'AATT_y
Cuts at: 1493 2717 3407 3520 3746 3898 6007 1493
Size: 1224 690 113 226 152 2109 1697
Fragments arranged by size:
2109 1697 1224 690 226 152 113
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 3069 3536 1870
Size: 73 1126 467 4545
Fragments arranged by size:
4545 1126 467 73
BamHI G'GATC_C
Cuts at: 3541 3908 3541
Size: 367 5844
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2118 2148 3180 3197 3532
Size: 325 217 627 331 30 1032 17 335
Cuts at: 3532 4987 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1032 627 335 331 325 217
30 17
BanII G_rGCy'C
Cuts at: 1391 2024 2145 2974 3530 1391
Size: 633 121 829 556 4072
Fragments arranged by size:
4072 829 633 556 121
BbeI G_GCGC'C
Cuts at: 2122 2152 2122
Size: 30 6181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4402 4436 1903
Size: 34 50 34 2381 34 3678
Fragments arranged by size:
3678 2381 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 4822 136
Size: 107 122 71 631 605 370 2780 1525
Fragments arranged by size:
2780 1525 631 605 370 122 107 71
BglII A'GATC_T
Cuts at: 6206 6206
Size: 6211
BlpI GC'TnA_GC
Cuts at: 3440 3628 3440
Size: 188 6023
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2145 4272 5428 1790
Size: 288 67 2127 1156 2573
Fragments arranged by size:
2573 2127 1156 288 67
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2081 3470 3942
Size: 416 53 83 399 24 664 1389 472
Cuts at: 3942 4900 442
Size: 958 1753
Fragments arranged by size:
1753 1389 958 664 472 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 3305 4871 1239
Size: 983 1083 1566 2579
Fragments arranged by size:
2579 1566 1083 983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4336 5201 5499 4336
Size: 865 298 5048
Fragments arranged by size:
5048 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4874 882
Size: 617 3375 2219
Fragments arranged by size:
3375 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4002 493
Size: 711 2798 2702
Fragments arranged by size:
2798 2702 711
BsaBI GATnn'nnATC
Cuts at: 2811 6205 2811
Size: 3394 2817
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2119 2149 4018 4400
Size: 53 83 186 936 586 30 1869 382
Cuts at: 4400 5035 275
Size: 635 1451
Fragments arranged by size:
1869 1451 936 635 586 382 186 83
53 30
BsaWI w'CCGG_w
Cuts at: 3271 4644 5042 5316 5463 5874 3271
Size: 1373 398 274 147 411 3608
Fragments arranged by size:
3608 1373 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5986
Fragments arranged by size:
5986 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2501 2534 1186
Size: 1315 33 4863
Fragments arranged by size:
4863 1315 33
BseYI C'CCAG_C
Cuts at: 3176 5414 5910 3176
Size: 2238 496 3477
Fragments arranged by size:
3477 2238 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3131 3212 1163
Size: 573 1395 81 4162
Fragments arranged by size:
4162 1395 573 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3062 4272 4357 5428 3062
Size: 1210 85 1071 3845
Fragments arranged by size:
3845 1210 1071 85
BsmI GAATG_Cn'
Cuts at: 2478 3722 3815 2478
Size: 1244 93 4874
Fragments arranged by size:
4874 1244 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2078 2145 2974 3062 3530 4272
Size: 399 234 54 67 829 88 468 742
Cuts at: 4272 4357 5428 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 829 742 468 399 234 88
85 67 54
BspEI T'CCGG_A
Cuts at: 3271 5874 3271
Size: 2603 3608
BspHI T'CATG_A
Cuts at: 4100 5830 4100
Size: 1730 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3195 3566 844
Size: 2351 371 3489
Fragments arranged by size:
3489 2351 371
BsrBI CCG'CTC
Cuts at: 1394 1823 4098 1394
Size: 429 2275 3507
Fragments arranged by size:
3507 2275 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4706 4880 65
Size: 1183 3458 174 1396
Fragments arranged by size:
3458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4855 5042 1700
Size: 110 3045 187 2869
Fragments arranged by size:
3045 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 4271 5283 4271
Size: 1012 5199
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6211
BstBI TT'CG_AA
Cuts at: 5015 5015
Size: 6211
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6211
BstXI CCAn_nnnn'nTGG
Cuts at: 2327 2327
Size: 6211
BstYI r'GATC_y
Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206
Size: 1569 367 385 17 1452 86 12 346
Cuts at: 6206 1972
Size: 1977
Fragments arranged by size:
1977 1569 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3504 3504
Size: 6211
BtgI C'CryG_G
Cuts at: 513 1065 2705 513
Size: 552 1640 4019
Fragments arranged by size:
4019 1640 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2030 2980 487
Size: 683 56 804 950 3718
Fragments arranged by size:
3718 950 804 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 3727 4521 4541 2052
Size: 1675 794 20 3722
Fragments arranged by size:
3722 1675 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6176
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DraIII CAC_nnn'GTG
Cuts at: 3246 3246
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5218 809
Size: 507 3902 1802
Fragments arranged by size:
3902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2395 3040 3363
Size: 54 1425 50 275 18 565 645 323
Cuts at: 3363 3658 4547 6176 8
Size: 295 889 1629 43
Fragments arranged by size:
1629 1425 889 645 565 323 295 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 2750 2933 4141 1353
Size: 1103 294 183 1208 3423
Fragments arranged by size:
3423 1208 1103 294 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4798 5172 5318 387
Size: 1064 138 3209 374 146 1280
Fragments arranged by size:
3209 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2526 2660 2731 3305 3400 3467
Size: 393 590 304 134 71 574 95 67
Cuts at: 3467 4274 4871 5658 5926 1239
Size: 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 590 574 393 304
268 134 95 71 67
EcoNI CCTnn'n_nnAGG
Cuts at: 3191 3191
Size: 6211
EcoO109I rG'GnC_Cy
Cuts at: 2141 2142 3235 3420 2141
Size: 1 1093 185 4932
Fragments arranged by size:
4932 1093 185 1
EcoRI G'AATT_C
Cuts at: 3520 3898 3520
Size: 378 5833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2321 2482 2321
Size: 161 6050
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 2122 2152 3227 5358 1678
Size: 444 30 1075 2131 2531
Fragments arranged by size:
2531 2131 1075 444 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2346 2378 4852
Size: 32 135 32 436 32 306 32 2474
Cuts at: 4852 4884 4926 4958 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2474 436 306 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3555 3801 669
Size: 988 281 1617 246 3079
Fragments arranged by size:
3079 1617 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6211
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KasI G'GCGC_C
Cuts at: 2118 2148 2118
Size: 30 6181
KpnI G_GTAC'C
Cuts at: 3184 3201 3536 3184
Size: 17 335 5859
Fragments arranged by size:
5859 335 17
MfeI C'AATT_G
Cuts at: 3810 3810
Size: 6211
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5325 5509 1305
Size: 4020 184 2007
Fragments arranged by size:
4020 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2397 3042 3365 10
Size: 54 1425 343 565 645 323 2856
Fragments arranged by size:
2856 1425 645 565 343 323 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4169 4528 4687 518
Size: 648 3003 359 159 2042
Fragments arranged by size:
3003 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2635 3627 4304 5452 5697 1606
Size: 606 423 992 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 606 423 245
NarI GG'CG_CC
Cuts at: 2119 2149 2119
Size: 30 6181
NcoI C'CATG_G
Cuts at: 513 1065 2705 513
Size: 552 1640 4019
Fragments arranged by size:
4019 1640 552
NdeI CA'TA_TG
Cuts at: 387 2784 387
Size: 2397 3814
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6211
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 2685 3348 3569 5114 1132
Size: 97 1456 663 221 1545 2229
Fragments arranged by size:
2229 1545 1456 663 221 97
PasI CC'CwG_GG
Cuts at: 1348 3147 1348
Size: 1799 4412
PciI A'CATG_T
Cuts at: 5110 5110
Size: 6211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3246 3283 1108
Size: 2138 37 4036
Fragments arranged by size:
4036 2138 37
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5793 5825 4280
Size: 32 1481 32 4666
Fragments arranged by size:
4666 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3235 3235
Size: 6211
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PspOMI G'GGCC_C
Cuts at: 2141 2141
Size: 6211
PstI C_TGCA'G
Cuts at: 830 2269 2821 2915 3563 830
Size: 1439 552 94 648 3478
Fragments arranged by size:
3478 1439 648 552 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 2212 2635 2212
Size: 423 5788
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SanDI GG'GwC_CC
Cuts at: 3235 3235
Size: 6211
SapI GCTCTTCn'nnn_
Cuts at: 2456 2456
Size: 6211
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 4459 6171 4459
Size: 1712 4499
SfcI C'TryA_G
Cuts at: 826 1045 2265 2817 2911 3559 4694 5375 5566
Size: 219 1220 552 94 648 1135 681 191
Cuts at: 5566 826
Size: 1471
Fragments arranged by size:
1471 1220 1135 681 648 552 219 191
94
SfoI GGC'GCC
Cuts at: 2120 2150 2120
Size: 30 6181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 3071 3538 1872
Size: 1199 467 4545
Fragments arranged by size:
4545 1199 467
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4315 5216 5478 820
Size: 197 926 2372 901 262 1553
Fragments arranged by size:
2372 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3348 3569 1132
Size: 2216 221 3774
Fragments arranged by size:
3774 2216 221
SspI AAT'ATT
Cuts at: 5 52 4135 5
Size: 47 4083 2081
Fragments arranged by size:
4083 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6211
StyI C'CwwG_G
Cuts at: 513 1065 2161 2705 3192 3650 513
Size: 552 1096 544 487 458 3074
Fragments arranged by size:
3074 1096 552 544 487 458
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4238 4255 4408 4593 5038 325
Size: 1255 2658 17 153 185 445 1498
Fragments arranged by size:
2658 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3013 4457 6169
Size: 274 80 33 51 1443 1036 1444 1712
Cuts at: 6169 96
Size: 138
Fragments arranged by size:
1712 1444 1443 1036 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2860 2892 3253 3285 6171 6203 2860
Size: 32 361 32 2886 32 2868
Fragments arranged by size:
2886 2868 361 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6211
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6211
XmaI C'CCGG_G
Cuts at: 1870 3069 3536 1870
Size: 1199 467 4545
Fragments arranged by size:
4545 1199 467
XmnI GAAnn'nnTTC
Cuts at: 2478 4340 2478
Size: 1862 4349
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI
XcmI