Restriction map for
pFN21AB5231
(Circular) (Six-base) MAPSORT of: pFN21AB5231.seq Check: 1491 from: 1 to: 6835
pFN21AB5231 6835 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2290 2290
Size: 6835
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4645 278
Size: 53 83 186 936 3109 2468
Fragments arranged by size:
3109 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3849 4178 1937
Size: 1912 329 4594
Fragments arranged by size:
4594 1912 329
Acc65I G'GTAC_C
Cuts at: 4156 4156
Size: 6835
AclI AA'CG_TT
Cuts at: 1563 4962 5335 1563
Size: 3399 373 3063
Fragments arranged by size:
3399 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2643 2898 3096 3494 3777 4898 6282 6550
Size: 1011 255 198 398 283 1121 1384 268
Cuts at: 6550 1632
Size: 1917
Fragments arranged by size:
1917 1384 1121 1011 398 283 268 255
198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6638
AflIII A'CryG_T
Cuts at: 1203 5734 6711 1203
Size: 4531 977 1327
Fragments arranged by size:
4531 1327 977
AgeI A'CCGG_T
Cuts at: 5666 5666
Size: 6835
AhdI GACnn_n'nnGTC
Cuts at: 5564 5564
Size: 6835
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6835
AlwNI CAG_nnn'CTG
Cuts at: 2540 2543 2876 3323 4578 6150 2540
Size: 3 333 447 1255 1572 3225
Fragments arranged by size:
3225 1572 1255 447 333 3
ApaLI G'TGCA_C
Cuts at: 4892 6048 4892
Size: 1156 5679
ApoI r'AATT_y
Cuts at: 1493 3047 3308 3625 4144 4370 4522 6631 1493
Size: 1554 261 317 519 226 152 2109 1697
Fragments arranged by size:
2109 1697 1554 519 317 261 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5389 160
Size: 872 4357 1606
Fragments arranged by size:
4357 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
AvaI C'yCGr_G
Cuts at: 1870 1943 4160 1870
Size: 73 2217 4545
Fragments arranged by size:
4545 2217 73
BamHI G'GATC_C
Cuts at: 4165 4532 4165
Size: 367 6468
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4156 5611 618
Size: 325 217 627 2369 1455 1842
Fragments arranged by size:
2369 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4154 1391
Size: 2763 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2297 2439 3084 3612 3903 4127 928
Size: 971 398 142 645 528 291 224 3636
Fragments arranged by size:
3636 971 645 528 398 291 224 142
BbvCI CC'TCA_GC
Cuts at: 2182 2703 2182
Size: 521 6314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2978 3012 5026 5060 1903
Size: 34 1041 34 2014 34 3678
Fragments arranged by size:
3678 2014 1041 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3742 4727 5937 3742
Size: 985 1210 4640
Fragments arranged by size:
4640 1210 985
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6835
BfrBI ATG'CAT
Cuts at: 3581 3581
Size: 6835
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2402 3887 4050
Size: 107 122 71 631 605 730 1485 163
Cuts at: 4050 5446 136
Size: 1396 1525
Fragments arranged by size:
1525 1485 1396 730 631 605 163 122
107 71
BglII A'GATC_T
Cuts at: 6830 6830
Size: 6835
BlpI GC'TnA_GC
Cuts at: 4252 4252
Size: 6835
Bme1580I G_kGCm'C
Cuts at: 1790 4896 6052 1790
Size: 3106 1156 2573
Fragments arranged by size:
3106 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6835
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3831 3943 4566
Size: 416 53 83 399 24 2414 112 623
Cuts at: 4566 5524 442
Size: 958 1753
Fragments arranged by size:
2414 1753 958 623 416 399 112 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2241 3104 3417 3689 5495 1239
Size: 1002 863 313 272 1806 2579
Fragments arranged by size:
2579 1806 1002 863 313 272
Bpu10I CC'TnA_GC
Cuts at: 2182 2703 2182
Size: 521 6314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4960 5825 6123 4960
Size: 865 298 5672
Fragments arranged by size:
5672 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5498 882
Size: 617 3999 2219
Fragments arranged by size:
3999 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4626 493
Size: 711 3422 2702
Fragments arranged by size:
3422 2702 711
BsaBI GATnn'nnATC
Cuts at: 6829 6829
Size: 6835
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4642 5024 5659 275
Size: 53 83 186 936 3109 382 635 1451
Fragments arranged by size:
3109 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5268 5666 5940 6087 6498 5268
Size: 398 274 147 411 5605
Fragments arranged by size:
5605 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2124 2154 3077 3107 1178
Size: 30 165 30 721 30 923 30 4906
Fragments arranged by size:
4906 923 721 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2049 2052 2462 2480 2489 2804 2807 2819
Size: 863 3 410 18 9 315 3 12
Cuts at: 2819 2822 3878 4029 4038 4080 4092 4101 4104
Size: 3 1056 151 9 42 12 9 3
Cuts at: 4104 4107 1186
Size: 3 3914
Fragments arranged by size:
3914 1056 863 410 315 151 42 18
12 12 9 9 9 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2392 6038 6534 2392
Size: 3646 496 2693
Fragments arranged by size:
3646 2693 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4285 5046 6074 1540
Size: 275 186 2284 761 1028 2301
Fragments arranged by size:
2301 2284 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4896 4981 6052 4896
Size: 85 1071 5679
Fragments arranged by size:
5679 1071 85
BsmI GAATG_Cn'
Cuts at: 2425 4346 4439 2425
Size: 1921 93 4821
Fragments arranged by size:
4821 1921 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4154 4896 4981 6052 1391
Size: 399 2364 742 85 1071 2174
Fragments arranged by size:
2364 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6498 6498
Size: 6835
BspHI T'CATG_A
Cuts at: 4724 6454 4724
Size: 1730 5105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2015 2290 4190 844
Size: 1171 275 1900 3489
Fragments arranged by size:
3489 1900 1171 275
BsrBI CCG'CTC
Cuts at: 1394 1823 4722 1394
Size: 429 2899 3507
Fragments arranged by size:
3507 2899 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3130 5330 5504 65
Size: 1183 1882 2200 174 1396
Fragments arranged by size:
2200 1882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5479 5666 1700
Size: 110 3669 187 2869
Fragments arranged by size:
3669 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6835
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6835
BssSI C'ACGA_G
Cuts at: 4895 5907 4895
Size: 1012 5823
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2282 2540 2543 794
Size: 1488 258 3 5086
Fragments arranged by size:
5086 1488 258 3
BstBI TT'CG_AA
Cuts at: 3603 5639 3603
Size: 2036 4799
BstEII G'GTnAC_C
Cuts at: 1184 2039 4097 1184
Size: 855 2058 3922
Fragments arranged by size:
3922 2058 855
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 2698 2053
Size: 645 6190
BstYI r'GATC_y
Cuts at: 1972 4165 4532 4917 4934 6386 6472 6484 6830
Size: 2193 367 385 17 1452 86 12 346
Cuts at: 6830 1972
Size: 1977
Fragments arranged by size:
2193 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3945 3945
Size: 6835
BtgI C'CryG_G
Cuts at: 513 1065 2004 3891 513
Size: 552 939 1887 3457
Fragments arranged by size:
3457 1887 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2540 3003 487
Size: 683 56 1314 463 4319
Fragments arranged by size:
4319 1314 683 463 56
BtsI GCAGTG_nn'
Cuts at: 2134 4351 5145 5165 2134
Size: 2217 794 20 3804
Fragments arranged by size:
3804 2217 794 20
ClaI AT'CG_AT
Cuts at: 1554 3366 1554
Size: 1812 5023
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2175 2210 529
Size: 35 1611 35 5154
Fragments arranged by size:
5154 1611 35 35
DraI TTT'AAA
Cuts at: 3624 4139 3624
Size: 515 6320
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5842 809
Size: 507 4526 1802
Fragments arranged by size:
4526 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2856 4282 5171
Size: 54 1425 50 275 18 1026 1426 889
Cuts at: 5171 6800 8
Size: 1629 43
Fragments arranged by size:
1629 1426 1425 1026 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4282 1537
Size: 275 2470 4090
Fragments arranged by size:
4090 2470 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2130 2439 2715 2796 2808 3513 3753 4765
Size: 777 309 276 81 12 705 240 1012
Cuts at: 4765 1353
Size: 3423
Fragments arranged by size:
3423 1012 777 705 309 276 240 81
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5422 5796 5942 387
Size: 1064 138 3833 374 146 1280
Fragments arranged by size:
3833 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2241 2643 2898 3096 3104 3417 3494
Size: 393 609 402 255 198 8 313 77
Cuts at: 3494 3689 3777 4898 5495 6282 6550 1239
Size: 195 88 1121 597 787 268 1524
Fragments arranged by size:
1524 1121 787 609 597 402 393 313
268 255 198 195 88 77 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3743 3743
Size: 6835
EcoO109I rG'GnC_Cy
Cuts at: 3680 3825 3680
Size: 145 6690
EcoRI G'AATT_C
Cuts at: 4144 4522 4144
Size: 378 6457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3524 3524
Size: 6835
FspI TGC'GCA
Cuts at: 5341 5341
Size: 6835
HaeII r_GCGC'y
Cuts at: 1678 5982 1678
Size: 4304 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2124 2156 3053
Size: 32 135 32 515 32 5 32 897
Cuts at: 3053 3085 3359 3391 5476 5508 5550 5582 1373
Size: 32 274 32 2085 32 42 32 2626
Fragments arranged by size:
2626 2085 897 515 274 135 42 32
32 32 32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4179 4425 669
Size: 988 281 2241 246 3079
Fragments arranged by size:
3079 2241 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3618 748
Size: 2870 3965
HpaI GTT'AAC
Cuts at: 4425 4425
Size: 6835
KpnI G_GTAC'C
Cuts at: 4160 4160
Size: 6835
MfeI C'AATT_G
Cuts at: 4434 4434
Size: 6835
MluI A'CGCG_T
Cuts at: 6711 6711
Size: 6835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2917 3075 5949 6133 1305
Size: 1612 158 2874 184 2007
Fragments arranged by size:
2874 2007 1612 184 158
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2858 10
Size: 54 1425 343 1026 3987
Fragments arranged by size:
3987 1425 1026 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4793 5152 5311 518
Size: 648 3627 359 159 2042
Fragments arranged by size:
3627 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2249 2540 4251 4928 6076 6321 1606
Size: 643 291 1711 677 1148 245 2120
Fragments arranged by size:
2120 1711 1148 677 643 291 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3891 513
Size: 552 939 1887 3457
Fragments arranged by size:
3457 1887 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6835
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6835
NotI GC'GGCC_GC
Cuts at: 4282 4282
Size: 6835
NruI TCG'CGA
Cuts at: 2018 2018
Size: 6835
NsiI A_TGCA'T
Cuts at: 3583 3583
Size: 6835
NspI r_CATG'y
Cuts at: 1132 1229 4193 5738 1132
Size: 97 2964 1545 2229
Fragments arranged by size:
2964 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3684 1348
Size: 2336 4499
PciI A'CATG_T
Cuts at: 5734 5734
Size: 6835
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6835
PmeI GTTT'AAAC
Cuts at: 4139 4139
Size: 6835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4904 4936 6417 6449 4904
Size: 32 1481 32 5290
Fragments arranged by size:
5290 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3680 3825 3680
Size: 145 6690
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6835
PsiI TTA'TAA
Cuts at: 4405 4405
Size: 6835
PstI C_TGCA'G
Cuts at: 830 4187 830
Size: 3357 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6835
PvuII CAG'CTG
Cuts at: 2249 2540 2249
Size: 291 6544
SalI G'TCGA_C
Cuts at: 1936 4177 1936
Size: 2241 4594
SapI GCTCTTCn'nnn_
Cuts at: 2808 2808
Size: 6835
SbfI CC_TGCA'GG
Cuts at: 4187 4187
Size: 6835
ScaI AGT'ACT
Cuts at: 5083 6795 5083
Size: 1712 5123
SfcI C'TryA_G
Cuts at: 826 1045 3421 3487 4183 5318 5999 6190 826
Size: 219 2376 66 696 1135 681 191 1471
Fragments arranged by size:
2376 1471 1135 696 681 219 191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6835
SmaI CCC'GGG
Cuts at: 1872 4162 1872
Size: 2290 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4939 5840 6102 820
Size: 197 926 2996 901 262 1553
Fragments arranged by size:
2996 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4626 493
Size: 4133 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6835
SphI G_CATG'C
Cuts at: 1132 4193 1132
Size: 3061 3774
SspI AAT'ATT
Cuts at: 5 52 4759 5
Size: 47 4707 2081
Fragments arranged by size:
4707 2081 47
StuI AGG'CCT
Cuts at: 1517 3738 1517
Size: 2221 4614
StyI C'CwwG_G
Cuts at: 513 1065 2004 2397 3882 3891 3927 4274 513
Size: 552 939 393 1485 9 36 347 3074
Fragments arranged by size:
3074 1485 939 552 393 347 36 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4862 4879 5032 5217 5662 325
Size: 1255 3282 17 153 185 445 1498
Fragments arranged by size:
3282 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3387 5081 6793
Size: 274 80 33 51 1443 1410 1694 1712
Cuts at: 6793 96
Size: 138
Fragments arranged by size:
1712 1694 1443 1410 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6795 6827 6795
Size: 32 6803
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6835
XbaI T'CTAG_A
Cuts at: 4171 4171
Size: 6835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3557 3889 3557
Size: 332 6503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6835
XmaI C'CCGG_G
Cuts at: 1870 4160 1870
Size: 2290 4545
XmnI GAAnn'nnTTC
Cuts at: 3507 4964 3507
Size: 1457 5378
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4643 276
Size: 53 83 186 936 3109 2468
Fragments arranged by size:
3109 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI