Restriction map for
pFN21AB5228
(Circular) (Six-base) MAPSORT of: pFN21AB5228.seq Check: 1035 from: 1 to: 6124
pFN21AB5228 6124 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2059 3374 2059
Size: 1315 4809
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3101 4187 5571 5839 1632
Size: 1469 1086 1384 268 1917
Fragments arranged by size:
1917 1469 1384 1086 268
AfeI AGC'GCT
Cuts at: 2724 2724
Size: 6124
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 3088 5023 6000 1203
Size: 1885 1935 977 1327
Fragments arranged by size:
1935 1885 1327 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 4853 4853
Size: 6124
AleI CACnn'nnGTG
Cuts at: 1166 3194 1166
Size: 2028 4096
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2098 2210 2679 1184
Size: 914 112 469 4629
Fragments arranged by size:
4629 914 469 112
AlwNI CAG_nnn'CTG
Cuts at: 2146 2437 2509 2767 3288 3867 5439 2146
Size: 291 72 258 521 579 1572 2831
Fragments arranged by size:
2831 1572 579 521 291 258 72
ApaI G_GGCC'C
Cuts at: 2444 3242 3420 2444
Size: 798 178 5148
Fragments arranged by size:
5148 798 178
ApaLI G'TGCA_C
Cuts at: 2654 4181 5337 2654
Size: 1527 1156 3441
Fragments arranged by size:
3441 1527 1156
ApoI r'AATT_y
Cuts at: 1493 3433 3659 3811 5920 1493
Size: 1940 226 152 2109 1697
Fragments arranged by size:
2109 1940 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 2443 2585 3203 3235 3449 1870
Size: 73 500 142 618 32 214 4545
Fragments arranged by size:
4545 618 500 214 142 73 32
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2857 3347 3445 4900
Size: 325 217 627 303 767 490 98 1455
Cuts at: 4900 618
Size: 1842
Fragments arranged by size:
1842 1455 767 627 490 325 303 217
98
BanII G_rGCy'C
Cuts at: 1391 2115 2444 3183 3212 3242 3420 3443 1391
Size: 724 329 739 29 30 178 23 4072
Fragments arranged by size:
4072 739 724 329 178 30 29 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3061 928
Size: 971 1162 3991
Fragments arranged by size:
3991 1162 971
BbvCI CC'TCA_GC
Cuts at: 2339 2339
Size: 6124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4315 4349 1903
Size: 34 2378 34 3678
Fragments arranged by size:
3678 2378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4016 5226 4016
Size: 1210 4914
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2886 2995 3114
Size: 107 122 71 631 605 1214 109 119
Cuts at: 3114 3297 3306 4735 136
Size: 183 9 1429 1525
Fragments arranged by size:
1525 1429 1214 631 605 183 122 119
109 107 71 9
BglII A'GATC_T
Cuts at: 6119 6119
Size: 6124
BlpI GC'TnA_GC
Cuts at: 3177 3541 3177
Size: 364 5760
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2444 2586 2658 2758 2809 2862 3242
Size: 370 284 142 72 100 51 53 380
Cuts at: 3242 3352 3420 4185 5341 1790
Size: 110 68 765 1156 2573
Fragments arranged by size:
2573 1156 765 380 370 284 142 110
100 72 68 53 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3855 4813 442
Size: 416 53 83 399 24 2438 958 1753
Fragments arranged by size:
2438 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2043 2345 2560 3065 4784 1239
Size: 804 302 215 505 1719 2579
Fragments arranged by size:
2579 1719 804 505 302 215
Bpu10I CC'TnA_GC
Cuts at: 2339 3343 2339
Size: 1004 5120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4249 5114 5412 4249
Size: 865 298 4961
Fragments arranged by size:
4961 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4787 882
Size: 617 3288 2219
Fragments arranged by size:
3288 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3915 493
Size: 711 2711 2702
Fragments arranged by size:
2711 2702 711
BsaBI GATnn'nnATC
Cuts at: 6118 6118
Size: 6124
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3931 4313 4948
Size: 53 83 186 936 482 1916 382 635
Cuts at: 4948 275
Size: 1451
Fragments arranged by size:
1916 1451 936 635 482 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2358 4557 4955 5229 5376 5787 2358
Size: 2199 398 274 147 411 2695
Fragments arranged by size:
2695 2199 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2558 2588 1178
Size: 30 165 30 1155 30 4714
Fragments arranged by size:
4714 1155 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2836 2839 2999 1186
Size: 1650 3 160 4311
Fragments arranged by size:
4311 1650 160 3
BseYI C'CCAG_C
Cuts at: 2136 2437 2992 3356 5327 5823 2136
Size: 301 555 364 1971 496 2437
Fragments arranged by size:
2437 1971 555 496 364 301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2180 2252 2393 2543 2591 2615 1163
Size: 573 444 72 141 150 48 24 4672
Fragments arranged by size:
4672 573 444 150 141 72 48 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2620 3574 4335 5363 1540
Size: 275 186 619 954 761 1028 2301
Fragments arranged by size:
2301 1028 954 761 619 275 186
BsiHKAI G_wGCw'C
Cuts at: 2658 4185 4270 5341 2658
Size: 1527 85 1071 3441
Fragments arranged by size:
3441 1527 1071 85
BsmI GAATG_Cn'
Cuts at: 3635 3728 3635
Size: 93 6031
BsmBI CGTCTCn'nnnn_
Cuts at: 2627 2627
Size: 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2160 2444 2586 2658 2758 2809
Size: 399 325 45 284 142 72 100 51
Cuts at: 2809 2862 3183 3212 3242 3352 3420 3443 4185
Size: 53 321 29 30 110 68 23 742
Cuts at: 4185 4270 5341 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 325 321 284 142
110 100 85 72 68 53 51 45
30 29 23
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 2688 4013 5743 2688
Size: 1325 1730 3069
Fragments arranged by size:
3069 1730 1325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2059 2493 2928 3374 3479 844
Size: 1215 434 435 446 105 3489
Fragments arranged by size:
3489 1215 446 435 434 105
BsrBI CCG'CTC
Cuts at: 1394 1823 3187 4011 1394
Size: 429 1364 824 3507
Fragments arranged by size:
3507 1364 824 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3079 4619 4793 65
Size: 1183 1831 1540 174 1396
Fragments arranged by size:
1831 1540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 2942 2966 4768 4955 1700
Size: 110 221 911 24 1802 187 2869
Fragments arranged by size:
2869 1802 911 221 187 110 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6124
BssHII G'CGCG_C
Cuts at: 1925 2590 1925
Size: 665 5459
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2146 2155 2509 3074 3288 794
Size: 1352 9 354 565 214 3630
Fragments arranged by size:
3630 1352 565 354 214 9
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6124
BstYI r'GATC_y
Cuts at: 1972 3454 3821 4206 4223 5675 5761 5773 6119
Size: 1482 367 385 17 1452 86 12 346
Cuts at: 6119 1972
Size: 1977
Fragments arranged by size:
1977 1482 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2768 2768
Size: 6124
BtgI C'CryG_G
Cuts at: 513 1065 2160 2383 2455 2599 2839 3131 513
Size: 552 1095 223 72 144 240 292 3506
Fragments arranged by size:
3506 1095 552 292 240 223 144 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 2507 3189 3201 3286 3640 4434 4454 2507
Size: 682 12 85 354 794 20 4177
Fragments arranged by size:
4177 794 682 354 85 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2680 2887 2937
Size: 54 1425 50 275 18 850 207 50
Cuts at: 2937 3571 4460 6089 8
Size: 634 889 1629 43
Fragments arranged by size:
1629 1425 889 850 634 275 207 54
50 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2558 4054 1353
Size: 650 555 1496 3423
Fragments arranged by size:
3423 1496 650 555
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 5085 5231 387
Size: 1064 138 3122 374 146 1280
Fragments arranged by size:
3122 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2345 2560 3065 3101 4187 4784
Size: 393 411 302 215 505 36 1086 597
Cuts at: 4784 5571 5839 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1086 787 597 505 411 393 302
268 215 36
EcoO109I rG'GnC_Cy
Cuts at: 2143 2737 3239 3417 2143
Size: 594 502 178 4850
Fragments arranged by size:
4850 594 502 178
EcoRI G'AATT_C
Cuts at: 3433 3811 3433
Size: 378 5746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3078 3078
Size: 6124
FspI TGC'GCA
Cuts at: 4630 4630
Size: 6124
HaeII r_GCGC'y
Cuts at: 1678 2726 3395 5271 1678
Size: 1048 669 1876 2531
Fragments arranged by size:
2531 1876 1048 669
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2683 2715 3020 3052 4765
Size: 32 135 32 1111 32 305 32 1713
Cuts at: 4765 4797 4839 4871 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1713 1111 305 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3468 3714 669
Size: 988 281 1530 246 3079
Fragments arranged by size:
3079 1530 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 3088 6000 3088
Size: 2912 3212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5238 5422 1305
Size: 3933 184 2007
Fragments arranged by size:
3933 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2939 10
Size: 54 1425 343 1107 3195
Fragments arranged by size:
3195 1425 1107 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 2661 3141 3194 4082 4441 4600
Size: 648 1108 387 480 53 888 359 159
Cuts at: 4600 518
Size: 2042
Fragments arranged by size:
2042 1108 888 648 480 387 359 159
53
MspA1I CmG'CkG
Cuts at: 1606 2021 2225 2332 2421 2457 2841 2992 3026
Size: 415 204 107 89 36 384 151 34
Cuts at: 3026 3312 3540 4217 5365 5610 1606
Size: 286 228 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 415 384 286 245 228
204 151 107 89 36 34
NaeI GCC'GGC
Cuts at: 2033 2033
Size: 6124
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5572
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NgoMIV G'CCGG_C
Cuts at: 2031 2031
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 2219 2666 3482 5027 1132
Size: 97 990 447 816 1545 2229
Fragments arranged by size:
2229 1545 990 816 447 97
PasI CC'CwG_GG
Cuts at: 1348 2787 3382 1348
Size: 1439 595 4090
Fragments arranged by size:
4090 1439 595
PciI A'CATG_T
Cuts at: 5023 5023
Size: 6124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2262 2389 1108
Size: 1154 127 4843
Fragments arranged by size:
4843 1154 127
PfoI T'CCnGG_A
Cuts at: 2322 2322
Size: 6124
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 5706 5738 4193
Size: 32 1481 32 4579
Fragments arranged by size:
4579 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PspOMI G'GGCC_C
Cuts at: 2440 3238 3416 2440
Size: 798 178 5148
Fragments arranged by size:
5148 798 178
PstI C_TGCA'G
Cuts at: 830 2061 2151 2433 2475 2490 2505 2763 2832
Size: 1231 90 282 42 15 15 258 69
Cuts at: 2832 2867 2925 3196 3371 3476 830
Size: 35 58 271 175 105 3478
Fragments arranged by size:
3478 1231 282 271 258 175 105 90
69 58 42 35 15 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
PvuII CAG'CTG
Cuts at: 2225 2332 2225
Size: 107 6017
SacII CC_GC'GG
Cuts at: 2458 2842 2458
Size: 384 5740
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SbfI CC_TGCA'GG
Cuts at: 2151 2433 2490 2925 3476 2151
Size: 282 57 435 551 4799
Fragments arranged by size:
4799 551 435 282 57
ScaI AGT'ACT
Cuts at: 3042 4372 6084 3042
Size: 1330 1712 3082
Fragments arranged by size:
3082 1712 1330
SfcI C'TryA_G
Cuts at: 826 1045 2057 2147 2429 2471 2486 2501 2759
Size: 219 1012 90 282 42 15 15 258
Cuts at: 2759 2828 2863 2921 3192 3367 3472 4607 5288
Size: 69 35 58 271 175 105 1135 681
Cuts at: 5288 5479 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1012 681 282 271 258 219
191 175 105 90 69 58 42 35
15 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 2445 3451 1872
Size: 573 1006 4545
Fragments arranged by size:
4545 1006 573
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4228 5129 5391 820
Size: 197 926 2285 901 262 1553
Fragments arranged by size:
2285 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 2666 3482 1132
Size: 1534 816 3774
Fragments arranged by size:
3774 1534 816
SspI AAT'ATT
Cuts at: 5 52 4048 5
Size: 47 3996 2081
Fragments arranged by size:
3996 2081 47
StuI AGG'CCT
Cuts at: 1517 2063 3035 1517
Size: 546 972 4606
Fragments arranged by size:
4606 972 546
StyI C'CwwG_G
Cuts at: 513 1065 2115 2677 3563 513
Size: 552 1050 562 886 3074
Fragments arranged by size:
3074 1050 886 562 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2035 2779 4151 4168 4321 4506 4951
Size: 1255 455 744 1372 17 153 185 445
Cuts at: 4951 325
Size: 1498
Fragments arranged by size:
1498 1372 1255 744 455 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3040 4370 6082
Size: 274 80 33 51 1443 1063 1330 1712
Cuts at: 6082 96
Size: 138
Fragments arranged by size:
1712 1443 1330 1063 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2538 2570 3210 3242 6084 6116 2538
Size: 32 640 32 2842 32 2546
Fragments arranged by size:
2842 2546 640 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2737 2820 2737
Size: 83 6041
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 2443 3449 1870
Size: 573 1006 4545
Fragments arranged by size:
4545 1006 573
XmnI GAAnn'nnTTC
Cuts at: 4253 4253
Size: 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI