Restriction map for
pFN21AB5220
(Circular) (Six-base) MAPSORT of: pFN21AB5220.seq Check: 3684 from: 1 to: 6811
pFN21AB5220 6811 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4621 278
Size: 53 83 186 936 3085 2468
Fragments arranged by size:
3085 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4154 1937
Size: 2217 4594
Acc65I G'GTAC_C
Cuts at: 4132 4132
Size: 6811
AclI AA'CG_TT
Cuts at: 1563 4938 5311 1563
Size: 3375 373 3063
Fragments arranged by size:
3375 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2906 3758 4874 6258 6526 1632
Size: 1274 852 1116 1384 268 1917
Fragments arranged by size:
1917 1384 1274 1116 852 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6614
AflIII A'CryG_T
Cuts at: 1203 3963 5710 6687 1203
Size: 2760 1747 977 1327
Fragments arranged by size:
2760 1747 1327 977
AgeI A'CCGG_T
Cuts at: 5642 5642
Size: 6811
AhdI GACnn_n'nnGTC
Cuts at: 5540 5540
Size: 6811
AleI CACnn'nnGTG
Cuts at: 1166 2885 1166
Size: 1719 5092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2903 1184
Size: 1719 5092
AlwNI CAG_nnn'CTG
Cuts at: 3152 3920 4554 6126 3152
Size: 768 634 1572 3837
Fragments arranged by size:
3837 1572 768 634
ApaLI G'TGCA_C
Cuts at: 4868 6024 4868
Size: 1156 5655
ApoI r'AATT_y
Cuts at: 1493 2941 4120 4346 4498 6607 1493
Size: 1448 1179 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 1179 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3057 5365 160
Size: 872 2025 2308 1606
Fragments arranged by size:
2308 2025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
AvaI C'yCGr_G
Cuts at: 1870 1943 3804 4136 1870
Size: 73 1861 332 4545
Fragments arranged by size:
4545 1861 332 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3900 3933 3900
Size: 33 6778
BamHI G'GATC_C
Cuts at: 4141 4508 4141
Size: 367 6444
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2064 4132 5587 618
Size: 325 217 627 277 2068 1455 1842
Fragments arranged by size:
2068 1842 1455 627 325 277 217
BanII G_rGCy'C
Cuts at: 1391 4130 1391
Size: 2739 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2909 3855 928
Size: 971 1010 946 3884
Fragments arranged by size:
3884 1010 971 946
BbvCI CC'TCA_GC
Cuts at: 3483 3483
Size: 6811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5002 5036 1903
Size: 34 50 34 2981 34 3678
Fragments arranged by size:
3678 2981 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4703 5913 4703
Size: 1210 5601
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6811
BfrBI ATG'CAT
Cuts at: 2722 2722
Size: 6811
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5422 136
Size: 107 122 71 631 605 3750 1525
Fragments arranged by size:
3750 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2752 3382 6806 2752
Size: 630 3424 2757
Fragments arranged by size:
3424 2757 630
BlpI GC'TnA_GC
Cuts at: 4228 4228
Size: 6811
Bme1580I G_kGCm'C
Cuts at: 1790 4872 6028 1790
Size: 3082 1156 2573
Fragments arranged by size:
3082 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6811
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3908 4542 5500
Size: 416 53 83 399 24 2491 634 958
Cuts at: 5500 442
Size: 1753
Fragments arranged by size:
2491 1753 958 634 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6811
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2805 3758 5471 1239
Size: 1566 953 1713 2579
Fragments arranged by size:
2579 1713 1566 953
Bpu10I CC'TnA_GC
Cuts at: 3483 3483
Size: 6811
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3587 4936 5801 6099 3587
Size: 1349 865 298 4299
Fragments arranged by size:
4299 1349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5474 882
Size: 617 3975 2219
Fragments arranged by size:
3975 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3964 4602 493
Size: 711 2760 638 2702
Fragments arranged by size:
2760 2702 711 638
BsaBI GATnn'nnATC
Cuts at: 6805 6805
Size: 6811
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4618 5000 5635 275
Size: 53 83 186 936 3085 382 635 1451
Fragments arranged by size:
3085 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2309 5244 5642 5916 6063 6474 2309
Size: 2935 398 274 147 411 2646
Fragments arranged by size:
2935 2646 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6586
Fragments arranged by size:
6586 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3311 3998 1186
Size: 2125 687 3999
Fragments arranged by size:
3999 2125 687
BseYI C'CCAG_C
Cuts at: 6014 6510 6014
Size: 496 6315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6238
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4261 5022 6050 1540
Size: 275 186 2260 761 1028 2301
Fragments arranged by size:
2301 2260 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4872 4957 6028 4872
Size: 85 1071 5655
Fragments arranged by size:
5655 1071 85
BsmI GAATG_Cn'
Cuts at: 2696 4322 4415 2696
Size: 1626 93 5092
Fragments arranged by size:
5092 1626 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4130 4872 4957 6028 1391
Size: 399 2340 742 85 1071 2174
Fragments arranged by size:
2340 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6474 6474
Size: 6811
BspHI T'CATG_A
Cuts at: 4700 6430 4700
Size: 1730 5081
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4099 4166 844
Size: 3255 67 3489
Fragments arranged by size:
3489 3255 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4698 1394
Size: 429 2875 3507
Fragments arranged by size:
3507 2875 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5306 5480 65
Size: 1183 4058 174 1396
Fragments arranged by size:
4058 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 5455 5642 1700
Size: 110 257 3388 187 2869
Fragments arranged by size:
3388 2869 257 187 110
BsrGI T'GTAC_A
Cuts at: 96 2146 96
Size: 2050 4761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6811
BssSI C'ACGA_G
Cuts at: 4871 5883 4871
Size: 1012 5799
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6811
BstBI TT'CG_AA
Cuts at: 5615 5615
Size: 6811
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6811
BstYI r'GATC_y
Cuts at: 1972 2752 3064 3382 3937 4141 4508 4893 4910
Size: 780 312 318 555 204 367 385 17
Cuts at: 4910 6362 6448 6460 6806 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 780 555 385 367 346 318
312 204 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2932 2932
Size: 6811
BtgI C'CryG_G
Cuts at: 513 1065 2331 513
Size: 552 1266 4993
Fragments arranged by size:
4993 1266 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6072
Fragments arranged by size:
6072 683 56
BtsI GCAGTG_nn'
Cuts at: 2701 4327 5121 5141 2701
Size: 1626 794 20 4371
Fragments arranged by size:
4371 1626 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6811
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6776
DraI TTT'AAA
Cuts at: 4115 4115
Size: 6811
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5818 809
Size: 507 4502 1802
Fragments arranged by size:
4502 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3474 4258 5147
Size: 54 1425 50 275 18 1644 784 889
Cuts at: 5147 6776 8
Size: 1629 43
Fragments arranged by size:
1644 1629 1425 889 784 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4258 1537
Size: 275 2446 4090
Fragments arranged by size:
4090 2446 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3180 4741 1353
Size: 1827 1561 3423
Fragments arranged by size:
3423 1827 1561
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5398 5772 5918 387
Size: 1064 138 3809 374 146 1280
Fragments arranged by size:
3809 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2805 2906 3758 4874 5471 6258 6526
Size: 393 1173 101 852 1116 597 787 268
Cuts at: 6526 1239
Size: 1524
Fragments arranged by size:
1524 1173 1116 852 787 597 393 268
101
EcoRI G'AATT_C
Cuts at: 4120 4498 4120
Size: 378 6433
EcoRV GAT'ATC
Cuts at: 3157 3157
Size: 6811
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3404 3515 3404
Size: 111 6700
FspI TGC'GCA
Cuts at: 5317 5317
Size: 6811
HaeII r_GCGC'y
Cuts at: 1678 2803 5958 1678
Size: 1125 3155 2531
Fragments arranged by size:
3155 2531 1125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2529 2561 3395 3427 3682
Size: 32 135 32 957 32 834 32 255
Cuts at: 3682 3714 3979 4011 5452 5484 5526 5558 1373
Size: 32 265 32 1441 32 42 32 2626
Fragments arranged by size:
2626 1441 957 834 265 255 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3466 4155 4401 669
Size: 988 281 1528 689 246 3079
Fragments arranged by size:
3079 1528 988 689 281 246
HindIII A'AGCT_T
Cuts at: 748 3122 748
Size: 2374 4437
HpaI GTT'AAC
Cuts at: 4401 4401
Size: 6811
KpnI G_GTAC'C
Cuts at: 4136 4136
Size: 6811
MfeI C'AATT_G
Cuts at: 4410 4410
Size: 6811
MluI A'CGCG_T
Cuts at: 6687 6687
Size: 6811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3178 3727 5925 6109 1305
Size: 1873 549 2198 184 2007
Fragments arranged by size:
2198 2007 1873 549 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3476 10
Size: 54 1425 343 1644 3345
Fragments arranged by size:
3345 1644 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2885 3843 4769 5128 5287 518
Size: 648 1719 958 926 359 159 2042
Fragments arranged by size:
2042 1719 958 926 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4227 4904 6052 6297 1606
Size: 2621 677 1148 245 2120
Fragments arranged by size:
2621 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2069 2069
Size: 6811
NcoI C'CATG_G
Cuts at: 513 1065 2331 513
Size: 552 1266 4993
Fragments arranged by size:
4993 1266 552
NdeI CA'TA_TG
Cuts at: 387 2299 387
Size: 1912 4899
NgoMIV G'CCGG_C
Cuts at: 2067 2067
Size: 6811
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6811
NotI GC'GGCC_GC
Cuts at: 4258 4258
Size: 6811
NsiI A_TGCA'T
Cuts at: 2724 2724
Size: 6811
NspI r_CATG'y
Cuts at: 1132 1229 2722 2726 4169 5714 1132
Size: 97 1493 4 1443 1545 2229
Fragments arranged by size:
2229 1545 1493 1443 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6811
PciI A'CATG_T
Cuts at: 5710 5710
Size: 6811
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6811
PmeI GTTT'AAAC
Cuts at: 4115 4115
Size: 6811
PmlI CAC'GTG
Cuts at: 3964 3964
Size: 6811
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4880 4912 6393 6425 4880
Size: 32 1481 32 5266
Fragments arranged by size:
5266 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6811
PsiI TTA'TAA
Cuts at: 2235 4381 2235
Size: 2146 4665
PstI C_TGCA'G
Cuts at: 830 4110 4163 830
Size: 3280 53 3478
Fragments arranged by size:
3478 3280 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6811
SalI G'TCGA_C
Cuts at: 1936 4153 1936
Size: 2217 4594
SbfI CC_TGCA'GG
Cuts at: 4163 4163
Size: 6811
ScaI AGT'ACT
Cuts at: 5059 6771 5059
Size: 1712 5099
SfcI C'TryA_G
Cuts at: 826 1045 2685 4106 4159 5294 5975 6166 826
Size: 219 1640 1421 53 1135 681 191 1471
Fragments arranged by size:
1640 1471 1421 1135 681 219 191 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6811
SmaI CCC'GGG
Cuts at: 1872 4138 1872
Size: 2266 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3566 4915 5816 6078 820
Size: 197 926 1623 1349 901 262 1553
Fragments arranged by size:
1623 1553 1349 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4602 493
Size: 4109 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6811
SphI G_CATG'C
Cuts at: 1132 4169 1132
Size: 3037 3774
SspI AAT'ATT
Cuts at: 5 52 2968 3037 4735 5
Size: 47 2916 69 1698 2081
Fragments arranged by size:
2916 2081 1698 69 47
StuI AGG'CCT
Cuts at: 1517 3732 1517
Size: 2215 4596
StyI C'CwwG_G
Cuts at: 513 1065 2331 2842 2862 4250 513
Size: 552 1266 511 20 1388 3074
Fragments arranged by size:
3074 1388 1266 552 511 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4838 4855 5008 5193 5638 325
Size: 1255 3258 17 153 185 445 1498
Fragments arranged by size:
3258 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2146 2266 3005
Size: 274 80 33 51 1443 169 120 739
Cuts at: 3005 5057 6769 96
Size: 2052 1712 138
Fragments arranged by size:
2052 1712 1443 739 274 169 138 120
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6771 6803 6771
Size: 32 6779
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6811
XbaI T'CTAG_A
Cuts at: 4147 4147
Size: 6811
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6811
XmaI C'CCGG_G
Cuts at: 1870 4136 1870
Size: 2266 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 2472 4069 4940 2340
Size: 132 1597 871 4211
Fragments arranged by size:
4211 1597 871 132
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4619 276
Size: 53 83 186 936 3085 2468
Fragments arranged by size:
3085 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NarI NruI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI