Restriction map for
pFN21AB5209
(Circular) (Six-base) MAPSORT of: pFN21AB5209.seq Check: 7300 from: 1 to: 6286
pFN21AB5209 6286 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3629 1937
Size: 1692 4594
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 3585 4413 4786 1563
Size: 2022 828 373 3063
Fragments arranged by size:
3063 2022 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 4349 5733 6001 1632
Size: 623 2094 1384 268 1917
Fragments arranged by size:
2094 1917 1384 623 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 2307 2677 3099 5185 6162 1203
Size: 1104 370 422 2086 977 1327
Fragments arranged by size:
2086 1327 1104 977 422 370
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2116 3182 3206 3356 3562 1184
Size: 932 1066 24 150 206 3908
Fragments arranged by size:
3908 1066 932 206 150 24
AlwNI CAG_nnn'CTG
Cuts at: 2857 4029 5601 2857
Size: 1172 1572 3542
Fragments arranged by size:
3542 1572 1172
ApaI G_GGCC'C
Cuts at: 2708 3509 2708
Size: 801 5485
ApaLI G'TGCA_C
Cuts at: 4343 5499 4343
Size: 1156 5130
ApoI r'AATT_y
Cuts at: 1493 2969 3595 3821 3973 6082 1493
Size: 1476 626 226 152 2109 1697
Fragments arranged by size:
2109 1697 1476 626 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 3611 1870
Size: 73 1668 4545
Fragments arranged by size:
4545 1668 73
BamHI G'GATC_C
Cuts at: 3424 3616 3983 3424
Size: 192 367 5727
Fragments arranged by size:
5727 367 192
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2100 3519 3607 5062 618
Size: 325 217 627 313 1419 88 1455 1842
Fragments arranged by size:
1842 1455 1419 627 325 313 217 88
BanII G_rGCy'C
Cuts at: 1391 2090 2708 2916 3393 3509 3605 1391
Size: 699 618 208 477 116 96 4072
Fragments arranged by size:
4072 699 618 477 208 116 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2120 2285 928
Size: 971 221 165 4929
Fragments arranged by size:
4929 971 221 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3538 3572 4477 4511 1903
Size: 34 1601 34 905 34 3678
Fragments arranged by size:
3678 1601 905 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 3367 1548
Size: 1819 4467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 2873 4897
Size: 107 122 71 631 605 365 836 2024
Cuts at: 4897 136
Size: 1525
Fragments arranged by size:
2024 1525 836 631 605 365 122 107
71
BglII A'GATC_T
Cuts at: 2802 6281 2802
Size: 3479 2807
BlpI GC'TnA_GC
Cuts at: 3703 3703
Size: 6286
Bme1580I G_kGCm'C
Cuts at: 1790 2708 3509 4347 5503 1790
Size: 918 801 838 1156 2573
Fragments arranged by size:
2573 1156 918 838 801
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3538 4017 4975
Size: 416 53 83 399 24 2121 479 958
Cuts at: 4975 442
Size: 1753
Fragments arranged by size:
2121 1753 958 479 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 2486 3152 3302 4946 1239
Size: 1172 75 666 150 1644 2579
Fragments arranged by size:
2579 1644 1172 666 150 75
Bpu10I CC'TnA_GC
Cuts at: 3147 3147
Size: 6286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3341 4411 5276 5574 3341
Size: 1070 865 298 4053
Fragments arranged by size:
4053 1070 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4949 882
Size: 617 3450 2219
Fragments arranged by size:
3450 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 3211 4077 493
Size: 711 1898 109 866 2702
Fragments arranged by size:
2702 1898 866 711 109
BsaBI GATnn'nnATC
Cuts at: 3404 6280 3404
Size: 2876 3410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2120 4093 4475 5110
Size: 53 83 186 936 587 1973 382 635
Cuts at: 5110 275
Size: 1451
Fragments arranged by size:
1973 1451 936 635 587 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3095 3125 1178
Size: 30 165 30 1692 30 4339
Fragments arranged by size:
4339 1692 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2270 2525 2528 2702 3149 1186
Size: 1081 3 255 3 174 447 4323
Fragments arranged by size:
4323 1081 447 255 174 3 3
BseYI C'CCAG_C
Cuts at: 2571 2935 5489 5985 2571
Size: 364 2554 496 2872
Fragments arranged by size:
2872 2554 496 364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3152 3178 3180 1163
Size: 573 1416 26 2 4269
Fragments arranged by size:
4269 1416 573 26 2
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2402 3053 4347 4432 5503 2402
Size: 651 1294 85 1071 3185
Fragments arranged by size:
3185 1294 1071 651 85
BsiWI C'GTAC_G
Cuts at: 3102 3102
Size: 6286
BsmI GAATG_Cn'
Cuts at: 2081 2607 3797 3890 2081
Size: 526 1190 93 4477
Fragments arranged by size:
4477 1190 526 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2402 2708 2916 3053 3393 3509
Size: 399 300 312 306 208 137 340 116
Cuts at: 3509 3605 4347 4432 5503 1391
Size: 96 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 312 306 300
208 137 116 96 85
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 4175 5905 4175
Size: 1730 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3641 844
Size: 2797 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3556 3562 4781 4955 65
Size: 1183 2308 6 1219 174 1396
Fragments arranged by size:
2308 1396 1219 1183 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 4930 5117 1700
Size: 110 241 2879 187 2869
Fragments arranged by size:
2879 2869 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 2927 4346 5358 2927
Size: 1419 1012 3855
Fragments arranged by size:
3855 1419 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2149 794
Size: 1355 4931
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 2986 1184
Size: 1802 4484
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 3130 3516 2035
Size: 1095 386 4805
Fragments arranged by size:
4805 1095 386
BstYI r'GATC_y
Cuts at: 1972 2259 2478 2802 3306 3405 3424 3616 3983
Size: 287 219 324 504 99 19 192 367
Cuts at: 3983 4368 4385 5837 5923 5935 6281 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 504 385 367 346 324 287
219 192 99 86 19 17 12
Bsu36I CC'TnA_GG
Cuts at: 2547 2547
Size: 6286
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5734
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5547
Fragments arranged by size:
5547 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2142 2143 3368 3802 4596 4616 2100
Size: 42 1 1225 434 794 20 3770
Fragments arranged by size:
3770 1225 794 434 42 20 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2520 2715
Size: 54 1425 50 275 18 195 495 195
Cuts at: 2715 3733 4622 6251 8
Size: 1018 889 1629 43
Fragments arranged by size:
1629 1425 1018 889 495 275 195 195
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 3072 4216 1353
Size: 668 1051 1144 3423
Fragments arranged by size:
3423 1144 1051 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2411 2486 3152 3302 4349 4946
Size: 393 623 156 75 666 150 1047 597
Cuts at: 4946 5733 6001 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1047 787 666 623 597 393 268
156 150 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 3131 3413 2978
Size: 153 282 5851
Fragments arranged by size:
5851 282 153
EcoO109I rG'GnC_Cy
Cuts at: 2705 2932 3505 2705
Size: 227 573 5486
Fragments arranged by size:
5486 573 227
EcoRI G'AATT_C
Cuts at: 2969 3595 3973 2969
Size: 626 378 5282
Fragments arranged by size:
5282 626 378
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 5433 1678
Size: 3755 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4927 4959 5001 5033 1373
Size: 32 135 32 3355 32 42 32 2626
Fragments arranged by size:
3355 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3630 3876 669
Size: 988 281 1692 246 3079
Fragments arranged by size:
3079 1692 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6286
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2148 3054 5400 5584 1305
Size: 843 906 2346 184 2007
Fragments arranged by size:
2346 2007 906 843 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2522 10
Size: 54 1425 343 195 495 3774
Fragments arranged by size:
3774 1425 495 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2612 4244 4603 4762 518
Size: 648 1446 1632 359 159 2042
Fragments arranged by size:
2042 1632 1446 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2222 2464 3702 4379 5527 5772 1606
Size: 616 242 1238 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 677 616 245 242
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 6286
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5734
NdeI CA'TA_TG
Cuts at: 387 2198 2723 387
Size: 1811 525 3950
Fragments arranged by size:
3950 1811 525
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2311 2681 2782 3166 3644 5189 1132
Size: 97 1082 370 101 384 478 1545 2229
Fragments arranged by size:
2229 1545 1082 478 384 370 101 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 2307 2677 5185 2307
Size: 370 2508 3408
Fragments arranged by size:
3408 2508 370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2668 3091 1108
Size: 1560 423 4303
Fragments arranged by size:
4303 1560 423
PfoI T'CCnGG_A
Cuts at: 2355 2355
Size: 6286
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PmlI CAC'GTG
Cuts at: 3211 3211
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2932 2932
Size: 6286
PshAI GACnn'nnGTC
Cuts at: 1613 2858 1613
Size: 1245 5041
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PspOMI G'GGCC_C
Cuts at: 2704 3505 2704
Size: 801 5485
PstI C_TGCA'G
Cuts at: 830 2154 3335 3638 830
Size: 1324 1181 303 3478
Fragments arranged by size:
3478 1324 1181 303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 2222 2464 2222
Size: 242 6044
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SfcI C'TryA_G
Cuts at: 826 1045 2150 3331 3634 4769 5450 5641 826
Size: 219 1105 1181 303 1135 681 191 1471
Fragments arranged by size:
1471 1181 1135 1105 681 303 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3320 4390 5291 5553 820
Size: 197 926 1377 1070 901 262 1553
Fragments arranged by size:
1553 1377 1070 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3166 3644 1132
Size: 2034 478 3774
Fragments arranged by size:
3774 2034 478
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 2666 1517
Size: 1149 5137
StyI C'CwwG_G
Cuts at: 513 1065 2062 2667 2796 3725 513
Size: 552 997 605 129 929 3074
Fragments arranged by size:
3074 997 929 605 552 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4313 4330 4483 4668 5113 325
Size: 1255 2733 17 153 185 445 1498
Fragments arranged by size:
2733 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4532 6244 96
Size: 274 80 33 51 1443 2555 1712 138
Fragments arranged by size:
2555 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6246 6278 6246
Size: 32 6254
Tth111I GACn'n_nGTC
Cuts at: 1316 2119 2210 1316
Size: 803 91 5392
Fragments arranged by size:
5392 803 91
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2854 2854
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 4415 4415
Size: 6286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI