Restriction map for
pFN21AB5207
(Circular) (Six-base) MAPSORT of: pFN21AB5207.seq Check: 3944 from: 1 to: 6292
pFN21AB5207 6292 bp
With 182 enzymes: SgfI *
February 27, 2009 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3482 3482
Size: 6292
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2223 4102 278
Size: 53 83 186 936 687 1879 2468
Fragments arranged by size:
2468 1879 936 687 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 1937
Size: 1698 4594
Acc65I G'GTAC_C
Cuts at: 3448 3613 3448
Size: 165 6127
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 2866 3476 3597 4355 5739 6007 1632
Size: 423 811 610 121 758 1384 268 1917
Fragments arranged by size:
1917 1384 811 758 610 423 268 121
AfeI AGC'GCT
Cuts at: 2802 2802
Size: 6292
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 5191 6168 1203
Size: 3988 977 1327
Fragments arranged by size:
3988 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6292
AlwNI CAG_nnn'CTG
Cuts at: 4035 5607 4035
Size: 1572 4720
ApaLI G'TGCA_C
Cuts at: 2391 3372 4349 5505 2391
Size: 981 977 1156 3178
Fragments arranged by size:
3178 1156 981 977
ApoI r'AATT_y
Cuts at: 1493 2403 2547 3601 3827 3979 6088 1493
Size: 910 144 1054 226 152 2109 1697
Fragments arranged by size:
2109 1697 1054 910 226 152 144
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 2844 3617 1870
Size: 73 901 773 4545
Fragments arranged by size:
4545 901 773 73
BamHI G'GATC_C
Cuts at: 3199 3229 3622 3989 3199
Size: 30 393 367 5502
Fragments arranged by size:
5502 393 367 30
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 3448 3613 5068 618
Size: 325 217 627 387 1274 165 1455 1842
Fragments arranged by size:
1842 1455 1274 627 387 325 217 165
BanII G_rGCy'C
Cuts at: 1391 3611 1391
Size: 2220 4072
BbeI G_GCGC'C
Cuts at: 2178 2178
Size: 6292
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3357 3415 928
Size: 971 1458 58 3805
Fragments arranged by size:
3805 1458 971 58
BbvCI CC'TCA_GC
Cuts at: 2508 3417 2508
Size: 909 5383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 2649 1548
Size: 1101 5191
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 4903 136
Size: 107 122 71 631 605 376 2855 1525
Fragments arranged by size:
2855 1525 631 605 376 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 2091 3174 3709 2091
Size: 1083 535 4674
Fragments arranged by size:
4674 1083 535
Bme1580I G_kGCm'C
Cuts at: 1790 2395 2845 3376 4353 5509 1790
Size: 605 450 531 977 1156 2573
Fragments arranged by size:
2573 1156 977 605 531 450
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2868 4023 4981
Size: 416 53 83 399 24 1451 1155 958
Cuts at: 4981 442
Size: 1753
Fragments arranged by size:
1753 1451 1155 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2585 1056
Size: 1529 4763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2499 2649 3290 4952 1239
Size: 1260 150 641 1662 2579
Fragments arranged by size:
2579 1662 1260 641 150
Bpu10I CC'TnA_GC
Cuts at: 2508 3417 2508
Size: 909 5383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4417 5282 5580 4417
Size: 865 298 5129
Fragments arranged by size:
5129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 2880 3455 4955 882
Size: 617 774 607 575 1500 2219
Fragments arranged by size:
2219 1500 774 617 607 575
BsaAI yAC'GTr
Cuts at: 493 1204 4083 493
Size: 711 2879 2702
Fragments arranged by size:
2879 2702 711
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 2220 4099 4481
Size: 53 83 186 936 642 45 1879 382
Cuts at: 4481 5116 275
Size: 635 1451
Fragments arranged by size:
1879 1451 936 642 635 382 186 83
53 45
BsaWI w'CCGG_w
Cuts at: 4725 5123 5397 5544 5955 4725
Size: 398 274 147 411 5062
Fragments arranged by size:
5062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6067
Fragments arranged by size:
6067 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6292
BseYI C'CCAG_C
Cuts at: 2086 2304 3178 5495 5991 2086
Size: 218 874 2317 496 2387
Fragments arranged by size:
2387 2317 874 496 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5719
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2324 2395 2839 3376 4353 4438 5509 2324
Size: 71 444 537 977 85 1071 3107
Fragments arranged by size:
3107 1071 977 537 444 85 71
BsmI GAATG_Cn'
Cuts at: 3583 3803 3896 3583
Size: 220 93 5979
Fragments arranged by size:
5979 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2324 2395 2839 2845 3376 3611 4353
Size: 399 534 71 444 6 531 235 742
Cuts at: 4353 4438 5509 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 534 531 444 399 235
85 71 6
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 2436 4181 5911 2436
Size: 1745 1730 2817
Fragments arranged by size:
2817 1745 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 3647 844
Size: 2638 165 3489
Fragments arranged by size:
3489 2638 165
BsrBI CCG'CTC
Cuts at: 1394 1823 2111 4179 1394
Size: 429 288 2068 3507
Fragments arranged by size:
3507 2068 429 288
BsrDI GCAATG_nn'
Cuts at: 65 1248 2740 3168 3315 4787 4961 65
Size: 1183 1492 428 147 1472 174 1396
Fragments arranged by size:
1492 1472 1396 1183 428 174 147
BsrFI r'CCGG_y
Cuts at: 1700 1810 4936 5123 1700
Size: 110 3126 187 2869
Fragments arranged by size:
3126 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3073 96
Size: 2977 3315
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 2838 4352 5364 2838
Size: 1514 1012 3766
Fragments arranged by size:
3766 1514 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstXI CCAn_nnnn'nTGG
Cuts at: 3019 3019
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 3059 3199 3229 3282 3622 3989 4374 4391
Size: 1087 140 30 53 340 367 385 17
Cuts at: 4391 5843 5929 5941 6287 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1087 385 367 346 340 140
86 53 30 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2683 3268 513
Size: 552 1618 585 3537
Fragments arranged by size:
3537 1618 585 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5553
Fragments arranged by size:
5553 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 3253 3808 4602 4622 2217
Size: 1036 555 794 20 3887
Fragments arranged by size:
3887 1036 794 555 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DraIII CAC_nnn'GTG
Cuts at: 2834 2834
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 3009 3739
Size: 54 1425 50 275 18 198 981 730
Cuts at: 3739 4628 6257 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 981 889 730 275 198 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3573 4222 1353
Size: 2220 649 3423
Fragments arranged by size:
3423 2220 649
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2499 2649 2866 3290 3476 3597
Size: 393 423 444 150 217 424 186 121
Cuts at: 3597 4355 4952 5739 6007 1239
Size: 758 597 787 268 1524
Fragments arranged by size:
1524 787 758 597 444 424 423 393
268 217 186 150 121
EcoO109I rG'GnC_Cy
Cuts at: 2049 2471 2721 3536 2049
Size: 422 250 815 4805
Fragments arranged by size:
4805 815 422 250
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
EcoRV GAT'ATC
Cuts at: 3313 3313
Size: 6292
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 2178 2804 5439 1678
Size: 500 626 2635 2531
Fragments arranged by size:
2635 2531 626 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2460 2492 2774 2806 3200
Size: 32 135 32 888 32 282 32 394
Cuts at: 3200 3232 3284 3316 3572 3604 4933 4965 5007
Size: 32 52 32 256 32 1329 32 42
Cuts at: 5007 5039 1373
Size: 32 2626
Fragments arranged by size:
2626 1329 888 394 282 256 135 52
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3360 3636 3882 669
Size: 988 281 1422 276 246 3079
Fragments arranged by size:
3079 1422 988 281 276 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KasI G'GCGC_C
Cuts at: 2174 2174
Size: 6292
KpnI G_GTAC'C
Cuts at: 3452 3617 3452
Size: 165 6127
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2138 5406 5590 1305
Size: 833 3268 184 2007
Fragments arranged by size:
3268 2007 833 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3011 10
Size: 54 1425 343 1179 3291
Fragments arranged by size:
3291 1425 1179 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 2528 3017 3141 3258 4250 4609
Size: 648 1147 215 489 124 117 992 359
Cuts at: 4609 4768 518
Size: 159 2042
Fragments arranged by size:
2042 1147 992 648 489 359 215 159
124 117
MspA1I CmG'CkG
Cuts at: 1606 2033 3194 3708 4385 5533 5778 1606
Size: 427 1161 514 677 1148 245 2120
Fragments arranged by size:
2120 1161 1148 677 514 427 245
NarI GG'CG_CC
Cuts at: 2175 2175
Size: 6292
NcoI C'CATG_G
Cuts at: 513 1065 2683 513
Size: 552 1618 4122
Fragments arranged by size:
4122 1618 552
NdeI CA'TA_TG
Cuts at: 387 3435 387
Size: 3048 3244
NheI G'CTAG_C
Cuts at: 1052 2581 1052
Size: 1529 4763
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3650 5195 1132
Size: 97 2421 1545 2229
Fragments arranged by size:
2421 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6292
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 1108
Size: 1203 5089
PfoI T'CCnGG_A
Cuts at: 3306 3306
Size: 6292
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2471 2721 3536 2471
Size: 250 815 5227
Fragments arranged by size:
5227 815 250
PshAI GACnn'nnGTC
Cuts at: 1613 2120 1613
Size: 507 5785
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PstI C_TGCA'G
Cuts at: 830 3291 3644 830
Size: 2461 353 3478
Fragments arranged by size:
3478 2461 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 3194 3194
Size: 6292
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SanDI GG'GwC_CC
Cuts at: 3536 3536
Size: 6292
SapI GCTCTTCn'nnn_
Cuts at: 3573 3573
Size: 6292
SbfI CC_TGCA'GG
Cuts at: 3291 3644 3291
Size: 353 5939
ScaI AGT'ACT
Cuts at: 2379 4540 6252 2379
Size: 2161 1712 2419
Fragments arranged by size:
2419 2161 1712
SexAI A'CCwGG_T
Cuts at: 2830 2830
Size: 6292
SfcI C'TryA_G
Cuts at: 826 1045 3287 3640 4775 5456 5647 826
Size: 219 2242 353 1135 681 191 1471
Fragments arranged by size:
2242 1471 1135 681 353 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2048 2048
Size: 6292
SfoI GGC'GCC
Cuts at: 2176 2176
Size: 6292
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 2846 3619 1872
Size: 974 773 4545
Fragments arranged by size:
4545 974 773
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4396 5297 5559 820
Size: 197 926 2453 901 262 1553
Fragments arranged by size:
2453 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 3521 1517
Size: 2004 4288
StyI C'CwwG_G
Cuts at: 513 1065 2595 2683 3731 513
Size: 552 1530 88 1048 3074
Fragments arranged by size:
3074 1530 1048 552 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 2954 4319 4336 4489 4674 5119
Size: 1255 723 651 1365 17 153 185 445
Cuts at: 5119 325
Size: 1498
Fragments arranged by size:
1498 1365 1255 723 651 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2377 2644 2905
Size: 274 80 33 51 1443 400 267 261
Cuts at: 2905 3073 3154 4538 6250 96
Size: 168 81 1384 1712 138
Fragments arranged by size:
1712 1443 1384 400 274 267 261 168
138 81 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6252 6284 6252
Size: 32 6260
Tth111I GACn'n_nGTC
Cuts at: 1316 3364 1316
Size: 2048 4244
XbaI T'CTAG_A
Cuts at: 3205 3628 3205
Size: 423 5869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 3175 3328 2290
Size: 885 153 5254
Fragments arranged by size:
5254 885 153
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 2844 3617 1870
Size: 974 773 4545
Fragments arranged by size:
4545 974 773
XmnI GAAnn'nnTTC
Cuts at: 2980 4421 2980
Size: 1441 4851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2221 4100 276
Size: 53 83 186 936 687 1879 2468
Fragments arranged by size:
2468 1879 936 687 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SrfI SwaI