Restriction map for
pFN21AB5199
(Circular) (Six-base) MAPSORT of: pFN21AB5199.seq Check: 1989 from: 1 to: 6061
pFN21AB5199 6061 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2739 2739
Size: 6061
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2940 3871 278
Size: 53 83 186 936 1404 931 2468
Fragments arranged by size:
2468 1404 936 931 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2583 2940 3404 1937
Size: 646 357 464 4594
Fragments arranged by size:
4594 646 464 357
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4124 5508 5776 1632
Size: 2492 1384 268 1917
Fragments arranged by size:
2492 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 2861 1166
Size: 1695 4366
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2675 2707 2675
Size: 32 6029
AlwNI CAG_nnn'CTG
Cuts at: 3124 3804 5376 3124
Size: 680 1572 3809
Fragments arranged by size:
3809 1572 680
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 3370 3596 3748 5857 1493
Size: 1877 226 152 2109 1697
Fragments arranged by size:
2109 1877 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3154 3386 1870
Size: 73 1211 232 4545
Fragments arranged by size:
4545 1211 232 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2830 3382 4837 618
Size: 325 217 627 279 764 552 1455 1842
Fragments arranged by size:
1842 1455 764 627 552 325 279 217
BanII G_rGCy'C
Cuts at: 1391 2105 3173 3380 1391
Size: 714 1068 207 4072
Fragments arranged by size:
4072 1068 714 207
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2953 928
Size: 971 1054 4036
Fragments arranged by size:
4036 1054 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2020 2396 2927
Size: 107 122 71 631 605 348 376 531
Cuts at: 2927 3243 4672 136
Size: 316 1429 1525
Fragments arranged by size:
1525 1429 631 605 531 376 348 316
122 107 71
BglII A'GATC_T
Cuts at: 2115 6056 2115
Size: 3941 2120
BlpI GC'TnA_GC
Cuts at: 2714 3478 2714
Size: 764 5297
Bme1580I G_kGCm'C
Cuts at: 1790 2835 2896 3256 4122 5278 1790
Size: 1045 61 360 866 1156 2573
Fragments arranged by size:
2573 1156 1045 866 360 61
BmgBI CAC'GTC
Cuts at: 1318 2413 3070 1318
Size: 1095 657 4309
Fragments arranged by size:
4309 1095 657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3352 3792 4750
Size: 416 53 83 399 24 1935 440 958
Cuts at: 4750 442
Size: 1753
Fragments arranged by size:
1935 1753 958 440 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2234 2570 3031 4721 1239
Size: 893 102 336 461 1690 2579
Fragments arranged by size:
2579 1690 893 461 336 102
Bpu10I CC'TnA_GC
Cuts at: 3229 3260 3229
Size: 31 6030
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2318 2809 3049 4724 882
Size: 617 819 491 240 1675 2219
Fragments arranged by size:
2219 1675 819 617 491 240
BsaAI yAC'GTr
Cuts at: 493 1204 2098 3852 493
Size: 711 894 1754 2702
Fragments arranged by size:
2702 1754 894 711
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2937 3111 3868 4250
Size: 53 83 186 936 1404 174 757 382
Cuts at: 4250 4885 275
Size: 635 1451
Fragments arranged by size:
1451 1404 936 757 635 382 186 174
83 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 2759 2789 1178
Size: 30 165 30 684 30 642 30 4450
Fragments arranged by size:
4450 684 642 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2890 1186
Size: 847 857 4357
Fragments arranged by size:
4357 857 847
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2261 2705 1163
Size: 573 525 444 4519
Fragments arranged by size:
4519 573 525 444
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3327 3511 4272 5300 1540
Size: 275 186 1326 184 761 1028 2301
Fragments arranged by size:
2301 1326 1028 761 275 186 184
BsiHKAI G_wGCw'C
Cuts at: 2610 4122 4207 5278 2610
Size: 1512 85 1071 3393
Fragments arranged by size:
3393 1512 1071 85
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
BsmBI CGTCTCn'nnnn_
Cuts at: 3197 3197
Size: 6061
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2610 2835 2896 3173 3256 3380
Size: 399 315 505 225 61 277 83 124
Cuts at: 3380 4122 4207 5278 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 505 399 315 277 225
124 85 83 61
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2739 3416 844
Size: 1895 677 3489
Fragments arranged by size:
3489 1895 677
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 2898 3228 3948 1394
Size: 429 212 863 330 720 3507
Fragments arranged by size:
3507 863 720 429 330 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2150 3107 3138 4705 4892 1700
Size: 110 340 957 31 1567 187 2869
Fragments arranged by size:
2869 1567 957 340 187 110 31
BsrGI T'GTAC_A
Cuts at: 96 2463 2751 96
Size: 2367 288 3406
Fragments arranged by size:
3406 2367 288
BssHII G'CGCG_C
Cuts at: 1925 2049 1925
Size: 124 5937
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 2884 2884
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 2115 2949 3391 3758 4143 4160 5612 5698
Size: 143 834 442 367 385 17 1452 86
Cuts at: 5698 5710 6056 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 834 442 385 367 346 143
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2062 2520 2614 2670 2708 2743
Size: 552 939 58 458 94 56 38 35
Cuts at: 2743 2859 2909 3357 513
Size: 116 50 448 3217
Fragments arranged by size:
3217 939 552 458 448 116 94 58
56 50 38 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2090 487
Size: 683 56 864 4458
Fragments arranged by size:
4458 864 683 56
BtsI GCAGTG_nn'
Cuts at: 3577 4371 4391 3577
Size: 794 20 5247
Fragments arranged by size:
5247 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DraIII CAC_nnn'GTG
Cuts at: 2413 2413
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2508 2698
Size: 54 1425 50 275 18 390 288 190
Cuts at: 2698 3126 3324 3508 4397 6026 8
Size: 428 198 184 889 1629 43
Fragments arranged by size:
1629 1425 889 428 390 288 275 198
190 184 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3324 3508 1537
Size: 275 1512 184 4090
Fragments arranged by size:
4090 1512 275 184
EarI CTCTTCn'nnn_
Cuts at: 1353 3095 3991 1353
Size: 1742 896 3423
Fragments arranged by size:
3423 1742 896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2234 2570 3031 4124 4721 5508
Size: 393 500 102 336 461 1093 597 787
Cuts at: 5508 5776 1239
Size: 268 1524
Fragments arranged by size:
1524 1093 787 597 500 461 393 336
268 102
EcoO109I rG'GnC_Cy
Cuts at: 2186 2186
Size: 6061
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 3148 5208 1678
Size: 1470 2060 2531
Fragments arranged by size:
2531 2060 1470
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2222 2254 4702 4734 4776
Size: 32 135 32 650 32 2448 32 42
Cuts at: 4776 4808 1373
Size: 32 2626
Fragments arranged by size:
2626 2448 650 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 2574 5175 5359 1305
Size: 825 444 2601 184 2007
Fragments arranged by size:
2601 2007 825 444 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 2510 2700 3128 10
Size: 54 1425 343 390 288 190 428 2943
Fragments arranged by size:
2943 1425 428 390 343 288 190 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2861 2882 4019 4378 4537 518
Size: 648 1101 594 21 1137 359 159 2042
Fragments arranged by size:
2042 1137 1101 648 594 359 159 21
MspA1I CmG'CkG
Cuts at: 1606 2064 2635 2696 2745 3477 4154 5302 5547
Size: 458 571 61 49 732 677 1148 245
Cuts at: 5547 1606
Size: 2120
Fragments arranged by size:
2120 1148 732 677 571 458 245 61
49
NaeI GCC'GGC
Cuts at: 2152 3109 3140 2152
Size: 957 31 5073
Fragments arranged by size:
5073 957 31
NcoI C'CATG_G
Cuts at: 513 1065 2004 2520 3357 513
Size: 552 939 516 837 3217
Fragments arranged by size:
3217 939 837 552 516
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NgoMIV G'CCGG_C
Cuts at: 2150 3107 3138 2150
Size: 957 31 5073
Fragments arranged by size:
5073 957 31
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2542 2773 3419 4964 1132
Size: 97 1313 231 646 1545 2229
Fragments arranged by size:
2229 1545 1313 646 231 97
PasI CC'CwG_GG
Cuts at: 1348 2329 1348
Size: 981 5080
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2620 2799 1108
Size: 1512 179 4370
Fragments arranged by size:
4370 1512 179
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PstI C_TGCA'G
Cuts at: 830 2445 2634 3413 830
Size: 1615 189 779 3478
Fragments arranged by size:
3478 1615 779 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2635 2635
Size: 6061
SacII CC_GC'GG
Cuts at: 2065 2746 2065
Size: 681 5380
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 2441 2630 3013 3175 3409 4544 5225
Size: 219 1396 189 383 162 234 1135 681
Cuts at: 5225 5416 826
Size: 191 1471
Fragments arranged by size:
1471 1396 1135 681 383 234 219 191
189 162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3156 3388 1872
Size: 1284 232 4545
Fragments arranged by size:
4545 1284 232
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4165 5066 5328 820
Size: 197 926 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 2542 3419 1132
Size: 1410 877 3774
Fragments arranged by size:
3774 1410 877
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 3033 3357 3500 513
Size: 552 939 516 513 324 143 3074
Fragments arranged by size:
3074 939 552 516 513 324 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4088 4105 4258 4443 4888 325
Size: 1255 2508 17 153 185 445 1498
Fragments arranged by size:
2508 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 2463 2751
Size: 274 80 33 51 1443 279 207 288
Cuts at: 2751 4307 6019 96
Size: 1556 1712 138
Fragments arranged by size:
1712 1556 1443 288 279 274 207 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2345 2377 6021 6053 2345
Size: 32 3644 32 2353
Fragments arranged by size:
3644 2353 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2579 1316
Size: 1263 4798
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 2518 2929 2326
Size: 192 411 5458
Fragments arranged by size:
5458 411 192
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3154 3386 1870
Size: 1284 232 4545
Fragments arranged by size:
4545 1284 232
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2938 3869 276
Size: 53 83 186 936 1404 931 2468
Fragments arranged by size:
2468 1404 936 931 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI