Restriction map for
pFN21AB5192
(Circular) (Six-base) MAPSORT of: pFN21AB5192.seq Check: 5129 from: 1 to: 6088
pFN21AB5192 6088 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2349 3431 1937
Size: 412 1082 4594
Fragments arranged by size:
4594 1082 412
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2173 2177 2746 3016 4151 5535 5803 1632
Size: 541 4 569 270 1135 1384 268 1917
Fragments arranged by size:
1917 1384 1135 569 541 270 268 4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 2630 3080 1166
Size: 1464 450 4174
Fragments arranged by size:
4174 1464 450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2044 3126 3345 3831 5403 2044
Size: 1082 219 486 1572 2729
Fragments arranged by size:
2729 1572 1082 486 219
ApaI G_GGCC'C
Cuts at: 2511 2639 2511
Size: 128 5960
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 2027 3397 3623 3775 5884 1493
Size: 534 1370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1370 534 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 3413 1870
Size: 73 195 1275 4545
Fragments arranged by size:
4545 1275 195 73
BamHI G'GATC_C
Cuts at: 3114 3418 3785 3114
Size: 304 367 5417
Fragments arranged by size:
5417 367 304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2449 2492 2711 3144 3409
Size: 325 217 627 662 43 219 433 265
Cuts at: 3409 4864 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 662 627 433 325 265 219
217 43
BanII G_rGCy'C
Cuts at: 1391 2511 2639 3217 3248 3330 3407 1391
Size: 1120 128 578 31 82 77 4072
Fragments arranged by size:
4072 1120 578 128 82 77 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2287 2494 928
Size: 971 388 207 4522
Fragments arranged by size:
4522 971 388 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3152 3186 4279 4313 1903
Size: 34 1215 34 1093 34 3678
Fragments arranged by size:
3678 1215 1093 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2402 2807 4699
Size: 107 122 71 631 605 730 405 1892
Cuts at: 4699 136
Size: 1525
Fragments arranged by size:
1892 1525 730 631 605 405 122 107
71
BglII A'GATC_T
Cuts at: 2088 6083 2088
Size: 3995 2093
BlpI GC'TnA_GC
Cuts at: 2237 3329 3505 2237
Size: 1092 176 4820
Fragments arranged by size:
4820 1092 176
Bme1580I G_kGCm'C
Cuts at: 1790 2383 2454 2495 2511 2639 2714 3149 4149
Size: 593 71 41 16 128 75 435 1000
Cuts at: 4149 5305 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1000 593 435 128 75 71
41 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3355 3819 4777
Size: 416 53 83 399 24 1938 464 958
Cuts at: 4777 442
Size: 1753
Fragments arranged by size:
1938 1753 958 464 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2152 4748 1239
Size: 913 2596 2579
Fragments arranged by size:
2596 2579 913
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3082 3879 493
Size: 711 1878 797 2702
Fragments arranged by size:
2702 1878 797 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2292 3895 4277 4912
Size: 53 83 186 936 759 1603 382 635
Cuts at: 4912 275
Size: 1451
Fragments arranged by size:
1603 1451 936 759 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3096 4521 4919 5193 5340 5751 3096
Size: 1425 398 274 147 411 3433
Fragments arranged by size:
3433 1425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3230 3260 1178
Size: 30 165 30 1827 30 4006
Fragments arranged by size:
4006 1827 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2843 3296 1186
Size: 931 726 453 3978
Fragments arranged by size:
3978 931 726 453
BseYI C'CCAG_C
Cuts at: 2070 3333 5291 5787 2070
Size: 1263 1958 496 2371
Fragments arranged by size:
2371 1958 1263 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 1163
Size: 573 462 5053
Fragments arranged by size:
5053 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3217 3330 4149 4234 5305 3217
Size: 113 819 85 1071 4000
Fragments arranged by size:
4000 1071 819 113 85
BsmI GAATG_Cn'
Cuts at: 2968 3599 3692 2968
Size: 631 93 5364
Fragments arranged by size:
5364 631 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3383 3383
Size: 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2383 2454 2495 2511 2639 2714 3149
Size: 399 593 71 41 16 128 75 435
Cuts at: 3149 3217 3248 3330 3407 4149 4234 5305 1391
Size: 68 31 82 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 593 435 399 128 85
82 77 75 71 68 41 31 16
BspEI T'CCGG_A
Cuts at: 3096 5751 3096
Size: 2655 3433
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2931 3443 844
Size: 2087 512 3489
Fragments arranged by size:
3489 2087 512
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2565 4732 4919 1700
Size: 110 755 2167 187 2869
Fragments arranged by size:
2869 2167 755 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 2255 4148 5160 2255
Size: 1893 1012 3183
Fragments arranged by size:
3183 1893 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2044 2371 794
Size: 1250 327 4511
Fragments arranged by size:
4511 1250 327
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 2672 2998 1184
Size: 1488 326 4274
Fragments arranged by size:
4274 1488 326
BstXI CCAn_nnnn'nTGG
Cuts at: 2632 2632
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2088 3114 3366 3418 3785 4170 4187 5639
Size: 116 1026 252 52 367 385 17 1452
Cuts at: 5639 5725 5737 6083 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 252 116
86 52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2715 2796 513
Size: 552 939 168 543 81 3805
Fragments arranged by size:
3805 939 552 543 168 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3155 487
Size: 683 56 1929 3420
Fragments arranged by size:
3420 1929 683 56
BtsI GCAGTG_nn'
Cuts at: 2412 3604 4398 4418 2412
Size: 1192 794 20 4082
Fragments arranged by size:
4082 1192 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2154 2189 529
Size: 35 1590 35 4428
Fragments arranged by size:
4428 1590 35 35
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2394 2799
Size: 54 1425 50 275 18 177 387 405
Cuts at: 2799 3219 3535 4424 6053 8
Size: 420 316 889 1629 43
Fragments arranged by size:
1629 1425 889 420 405 387 316 275
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2651 4018 1353
Size: 1298 1367 3423
Fragments arranged by size:
3423 1367 1298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2960 4675 5049 5195 387
Size: 1064 138 1371 1715 374 146 1280
Fragments arranged by size:
1715 1371 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3215 3328 3215
Size: 113 5975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2173 2177 2746 3016 4151 4748
Size: 393 520 21 4 569 270 1135 597
Cuts at: 4748 5535 5803 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1135 787 597 569 520 393 270
268 21 4
EcoO109I rG'GnC_Cy
Cuts at: 2077 2508 2636 2851 2979 2077
Size: 431 128 215 128 5186
Fragments arranged by size:
5186 431 215 128 128
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
EcoRV GAT'ATC
Cuts at: 2517 2517
Size: 6088
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 3018 3106 5235 1678
Size: 1340 88 2129 2531
Fragments arranged by size:
2531 2129 1340 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3351 3383 4729 4761 4803
Size: 32 135 32 1779 32 1346 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2626 1779 1346 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2396 3221 10
Size: 54 1425 343 177 387 825 2877
Fragments arranged by size:
2877 1425 825 387 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 3080 4046 4405 4564 518
Size: 648 1464 450 966 359 159 2042
Fragments arranged by size:
2042 1464 966 648 450 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 3333 3504 4181 5329 5574 1606
Size: 606 1121 171 677 1148 245 2120
Fragments arranged by size:
2120 1148 1121 677 606 245 171
NcoI C'CATG_G
Cuts at: 513 1065 2004 2715 513
Size: 552 939 711 3886
Fragments arranged by size:
3886 939 711 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2165 3092 3446 4991 1132
Size: 97 936 927 354 1545 2229
Fragments arranged by size:
2229 1545 936 927 354 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3345 1108
Size: 2237 3851
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2851 2979 2851
Size: 128 5960
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 2507 2635 2507
Size: 128 5960
PstI C_TGCA'G
Cuts at: 830 2577 3440 830
Size: 1747 863 3478
Fragments arranged by size:
3478 1747 863
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 2212 3333 2212
Size: 1121 4967
SacI G_AGCT'C
Cuts at: 3217 3330 3217
Size: 113 5975
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2573 3436 4571 5252 5443 826
Size: 219 1528 863 1135 681 191 1471
Fragments arranged by size:
1528 1471 1135 863 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4192 5093 5355 820
Size: 197 926 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 2165 3446 1132
Size: 1033 1281 3774
Fragments arranged by size:
3774 1281 1033
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2004 2545 2715 3527 513
Size: 552 939 541 170 812 3074
Fragments arranged by size:
3074 939 812 552 541 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 2880 4115 4132 4285 4470 4915
Size: 1255 741 559 1235 17 153 185 445
Cuts at: 4915 325
Size: 1498
Fragments arranged by size:
1498 1255 1235 741 559 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3106 3138 6048 6080 3106
Size: 32 2910 32 3114
Fragments arranged by size:
3114 2910 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2860 1316
Size: 1544 4544
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2713 2803 3229 2713
Size: 90 426 5572
Fragments arranged by size:
5572 426 90
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI