Restriction map for
pFN21AB5190
(Circular) (Six-base) MAPSORT of: pFN21AB5190.seq Check: 1583 from: 1 to: 6469
pFN21AB5190 6469 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3740 3740
Size: 6469
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3812 1937
Size: 1875 4594
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2487 2826 3170 3432 3609 3701 4532 5916
Size: 855 339 344 262 177 92 831 1384
Cuts at: 5916 6184 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 855 831 344 339 268 262
177 92
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6272
AflIII A'CryG_T
Cuts at: 1203 5368 6345 1203
Size: 4165 977 1327
Fragments arranged by size:
4165 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 5198 5198
Size: 6469
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2422 1184
Size: 1238 5231
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 6437
AlwNI CAG_nnn'CTG
Cuts at: 2396 4212 5784 2396
Size: 1816 1572 3081
Fragments arranged by size:
3081 1816 1572
ApaLI G'TGCA_C
Cuts at: 4526 5682 4526
Size: 1156 5313
ApoI r'AATT_y
Cuts at: 1493 2521 2655 3219 3778 4004 4156 6265 1493
Size: 1028 134 564 559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 564 559 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 5023 160
Size: 872 3991 1606
Fragments arranged by size:
3991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 2789 3572 3794 1870
Size: 73 846 783 222 4545
Fragments arranged by size:
4545 846 783 222 73
BamHI G'GATC_C
Cuts at: 3799 4166 3799
Size: 367 6102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 3080 3122 3728 3790
Size: 325 217 627 1064 229 42 606 62
Cuts at: 3790 5245 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1064 627 606 325 229 217
62 42
BanII G_rGCy'C
Cuts at: 1391 2548 3788 1391
Size: 1157 1240 4072
Fragments arranged by size:
4072 1240 1157
BbeI G_GCGC'C
Cuts at: 3126 3126
Size: 6469
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 2814 2934 3360 928
Size: 971 555 360 120 426 4037
Fragments arranged by size:
4037 971 555 426 360 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4660 4694 1903
Size: 34 50 34 2639 34 3678
Fragments arranged by size:
3678 2639 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2145 2707 4361 5571 2145
Size: 562 1654 1210 3043
Fragments arranged by size:
3043 1654 1210 562
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5080 136
Size: 107 122 71 631 605 3408 1525
Fragments arranged by size:
3408 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 2410 3886 2410
Size: 1476 4993
Bme1580I G_kGCm'C
Cuts at: 1790 4530 5686 1790
Size: 2740 1156 2573
Fragments arranged by size:
2740 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2534 1318
Size: 1216 5253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2016 2384 4200
Size: 416 53 83 399 24 599 368 1816
Cuts at: 4200 5158 442
Size: 958 1753
Fragments arranged by size:
1816 1753 958 599 416 399 368 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2418 1056
Size: 1362 5107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2986 3215 3325 5129 1239
Size: 1747 229 110 1804 2579
Fragments arranged by size:
2579 1804 1747 229 110
Bpu10I CC'TnA_GC
Cuts at: 3060 3060
Size: 6469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4594 5459 5757 4594
Size: 865 298 5306
Fragments arranged by size:
5306 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5132 882
Size: 617 3633 2219
Fragments arranged by size:
3633 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4260 493
Size: 711 3056 2702
Fragments arranged by size:
3056 2702 711
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3123 4276 4658 5293
Size: 53 83 186 936 1590 1153 382 635
Cuts at: 5293 275
Size: 1451
Fragments arranged by size:
1590 1451 1153 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4902 5300 5574 5721 6132 4902
Size: 398 274 147 411 5239
Fragments arranged by size:
5239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6244
Fragments arranged by size:
6244 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2053 2234 2237 2384 2839 1186
Size: 867 181 3 147 455 4816
Fragments arranged by size:
4816 867 455 181 147 3
BseYI C'CCAG_C
Cuts at: 2231 2324 2537 3015 5672 6168 2231
Size: 93 213 478 2657 496 2532
Fragments arranged by size:
2657 2532 496 478 213 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2514 2610 1163
Size: 573 778 96 5022
Fragments arranged by size:
5022 778 573 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2048 2249 3919 4680 5708 1540
Size: 275 186 47 201 1670 761 1028 2301
Fragments arranged by size:
2301 1670 1028 761 275 201 186 47
BsiHKAI G_wGCw'C
Cuts at: 4530 4615 5686 4530
Size: 85 1071 5313
Fragments arranged by size:
5313 1071 85
BsmI GAATG_Cn'
Cuts at: 3980 4073 3980
Size: 93 6376
BsmBI CGTCTCn'nnnn_
Cuts at: 2851 2851
Size: 6469
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2548 3788 4530 4615 5686 1391
Size: 399 758 1240 742 85 1071 2174
Fragments arranged by size:
2174 1240 1071 758 742 399 85
BspEI T'CCGG_A
Cuts at: 6132 6132
Size: 6469
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2536 3740 3824 844
Size: 1692 1204 84 3489
Fragments arranged by size:
3489 1692 1204 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3043 4356 1394
Size: 429 1220 1313 3507
Fragments arranged by size:
3507 1313 1220 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3151 3486 4964 5138 65
Size: 1183 1903 335 1478 174 1396
Fragments arranged by size:
1903 1478 1396 1183 335 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5113 5300 1700
Size: 110 3303 187 2869
Fragments arranged by size:
3303 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 3281 4529 5541 3281
Size: 1248 1012 4209
Fragments arranged by size:
4209 1248 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6469
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6469
BstXI CCAn_nnnn'nTGG
Cuts at: 2744 2744
Size: 6469
BstYI r'GATC_y
Cuts at: 1972 3576 3799 4166 4551 4568 6020 6106 6118
Size: 1604 223 367 385 17 1452 86 12
Cuts at: 6118 6464 1972
Size: 346 1977
Fragments arranged by size:
1977 1604 1452 385 367 346 223 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2871 513
Size: 552 1806 4111
Fragments arranged by size:
4111 1806 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5730
Fragments arranged by size:
5730 683 56
BtsI GCAGTG_nn'
Cuts at: 3262 3522 3985 4779 4799 3262
Size: 260 463 794 20 4932
Fragments arranged by size:
4932 794 463 260 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 2278 3773 2278
Size: 1495 4974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 809
Size: 507 4160 1802
Fragments arranged by size:
4160 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2874 3531 3916
Size: 54 1425 50 275 18 1044 657 385
Cuts at: 3916 4805 6434 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1044 889 657 385 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2164 2752 3033 3186 3189 3573 4399 1353
Size: 811 588 281 153 3 384 826 3423
Fragments arranged by size:
3423 826 811 588 384 281 153 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2127 5056 5430 5576 387
Size: 1064 138 538 2929 374 146 1280
Fragments arranged by size:
2929 1280 1064 538 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2487 2826 2986 3170 3215 3325 3432
Size: 393 855 339 160 184 45 110 107
Cuts at: 3432 3609 3701 4532 5129 5916 6184 1239
Size: 177 92 831 597 787 268 1524
Fragments arranged by size:
1524 855 831 787 597 393 339 268
184 177 160 110 107 92 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3473 3473
Size: 6469
EcoRI G'AATT_C
Cuts at: 3778 4156 3778
Size: 378 6091
FspI TGC'GCA
Cuts at: 3498 4975 3498
Size: 1477 4992
HaeII r_GCGC'y
Cuts at: 1678 3126 5616 1678
Size: 1448 2490 2531
Fragments arranged by size:
2531 2490 1448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2915 2944 2947 2976 3185
Size: 32 135 32 1343 29 3 29 209
Cuts at: 3185 3217 5110 5142 5184 5216 1373
Size: 32 1893 32 42 32 2626
Fragments arranged by size:
2626 1893 1343 209 135 42 32 32
32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3813 4059 669
Size: 988 281 1875 246 3079
Fragments arranged by size:
3079 1875 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6469
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KasI G'GCGC_C
Cuts at: 3122 3122
Size: 6469
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2948 5583 5767 1305
Size: 1643 2635 184 2007
Fragments arranged by size:
2635 2007 1643 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3533 10
Size: 54 1425 343 1701 2946
Fragments arranged by size:
2946 1701 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2742 2901 3243 3449 4427 4786 4945
Size: 648 1576 159 342 206 978 359 159
Cuts at: 4945 518
Size: 2042
Fragments arranged by size:
2042 1576 978 648 359 342 206 159
159
MspA1I CmG'CkG
Cuts at: 1606 3747 3885 4562 5710 5955 1606
Size: 2141 138 677 1148 245 2120
Fragments arranged by size:
2141 2120 1148 677 245 138
NarI GG'CG_CC
Cuts at: 3123 3123
Size: 6469
NcoI C'CATG_G
Cuts at: 513 1065 2871 513
Size: 552 1806 4111
Fragments arranged by size:
4111 1806 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 2414 1052
Size: 1362 5107
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 3754 3827 5372 1132
Size: 97 2525 73 1545 2229
Fragments arranged by size:
2525 2229 1545 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6469
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3481 1108
Size: 2373 4096
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PstI C_TGCA'G
Cuts at: 830 2401 3821 830
Size: 1571 1420 3478
Fragments arranged by size:
3478 1571 1420
PvuI CG_AT'CG
Cuts at: 2001 2048 2001
Size: 47 6422
SalI G'TCGA_C
Cuts at: 1936 3811 1936
Size: 1875 4594
SapI GCTCTTCn'nnn_
Cuts at: 3033 3186 3573 3033
Size: 153 387 5929
Fragments arranged by size:
5929 387 153
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 2698 4717 6429 2698
Size: 2019 1712 2738
Fragments arranged by size:
2738 2019 1712
SfcI C'TryA_G
Cuts at: 826 1045 2397 3634 3817 4952 5633 5824 826
Size: 219 1352 1237 183 1135 681 191 1471
Fragments arranged by size:
1471 1352 1237 1135 681 219 191 183
SfoI GGC'GCC
Cuts at: 3124 3124
Size: 6469
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4573 5474 5736 820
Size: 197 926 2630 901 262 1553
Fragments arranged by size:
2630 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3827 1132
Size: 2695 3774
SspI AAT'ATT
Cuts at: 5 52 2110 4393 5
Size: 47 2058 2283 2081
Fragments arranged by size:
2283 2081 2058 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6469
StyI C'CwwG_G
Cuts at: 513 1065 2871 3224 3908 513
Size: 552 1806 353 684 3074
Fragments arranged by size:
3074 1806 684 552 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4496 4513 4666 4851 5296 325
Size: 1255 2916 17 153 185 445 1498
Fragments arranged by size:
2916 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 2696 4715
Size: 274 80 33 51 1443 171 548 2019
Cuts at: 4715 6427 96
Size: 1712 138
Fragments arranged by size:
2019 1712 1443 548 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2684 2716 2884 2916 6429 6461 2684
Size: 32 168 32 3513 32 2692
Fragments arranged by size:
3513 2692 168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2390 1316
Size: 1074 5395
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3424 3424
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6469
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 3168 4598 3168
Size: 1430 5039
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI