Restriction map for
pFN21AB5188
(Circular) (Six-base) MAPSORT of: pFN21AB5188.seq Check: 2978 from: 1 to: 5971
pFN21AB5188 5971 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2921 3781 278
Size: 53 83 186 936 1385 860 2468
Fragments arranged by size:
2468 1385 936 860 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3081 3314 1937
Size: 1144 233 4594
Fragments arranged by size:
4594 1144 233
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4034 5418 5686 1632
Size: 2402 1384 268 1917
Fragments arranged by size:
2402 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 2696 2741 1166
Size: 1530 45 4396
Fragments arranged by size:
4396 1530 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2806 2819 2972 1184
Size: 1622 13 153 4183
Fragments arranged by size:
4183 1622 153 13
AlwNI CAG_nnn'CTG
Cuts at: 2067 2136 2819 3714 5286 2067
Size: 69 683 895 1572 2752
Fragments arranged by size:
2752 1572 895 683 69
ApaI G_GGCC'C
Cuts at: 2719 2719
Size: 5971
ApaLI G'TGCA_C
Cuts at: 2557 4028 5184 2557
Size: 1471 1156 3344
Fragments arranged by size:
3344 1471 1156
ApoI r'AATT_y
Cuts at: 1493 3280 3506 3658 5767 1493
Size: 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3296 1870
Size: 73 1353 4545
Fragments arranged by size:
4545 1353 73
BamHI G'GATC_C
Cuts at: 2901 3301 3668 2901
Size: 400 367 5204
Fragments arranged by size:
5204 400 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2354 3019 3266 3292 4747
Size: 325 217 627 567 665 247 26 1455
Cuts at: 4747 618
Size: 1842
Fragments arranged by size:
1842 1455 665 627 567 325 247 217
26
BanII G_rGCy'C
Cuts at: 1391 2174 2719 3290 1391
Size: 783 545 571 4072
Fragments arranged by size:
4072 783 571 545
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 2995 928
Size: 971 192 904 3904
Fragments arranged by size:
3904 971 904 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2968 3002 4162 4196 1903
Size: 34 1031 34 1160 34 3678
Fragments arranged by size:
3678 1160 1031 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2370 4582 136
Size: 107 122 71 631 605 698 2212 1525
Fragments arranged by size:
2212 1525 698 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2476 5966 2476
Size: 3490 2481
BlpI GC'TnA_GC
Cuts at: 2173 3388 2173
Size: 1215 4756
Bme1580I G_kGCm'C
Cuts at: 1790 2561 2719 3022 4032 5188 1790
Size: 771 158 303 1010 1156 2573
Fragments arranged by size:
2573 1156 1010 771 303 158
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3040 3702 4660
Size: 416 53 83 399 24 1623 662 958
Cuts at: 4660 442
Size: 1753
Fragments arranged by size:
1753 1623 958 662 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2188 2193 2188
Size: 5 5966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2158 4631 1239
Size: 919 2473 2579
Fragments arranged by size:
2579 2473 919
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2543 4634 882
Size: 617 1044 2091 2219
Fragments arranged by size:
2219 2091 1044 617
BsaAI yAC'GTr
Cuts at: 493 1204 2896 3762 493
Size: 711 1692 866 2702
Fragments arranged by size:
2702 1692 866 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2918 3778 4160 4795
Size: 53 83 186 936 1385 860 382 635
Cuts at: 4795 275
Size: 1451
Fragments arranged by size:
1451 1385 936 860 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2673 4404 4802 5076 5223 5634 2673
Size: 1731 398 274 147 411 3010
Fragments arranged by size:
3010 1731 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2540 2570 2769 2799 1178
Size: 30 165 30 1137 30 199 30 4350
Fragments arranged by size:
4350 1137 199 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2292 2471 3260 1186
Size: 1106 179 789 3897
Fragments arranged by size:
3897 1106 789 179
BseYI C'CCAG_C
Cuts at: 2940 3015 5174 5670 2940
Size: 75 2159 496 3241
Fragments arranged by size:
3241 2159 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 2802 1163
Size: 573 672 394 4332
Fragments arranged by size:
4332 672 573 394
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2199 2406 3421 4182 5210 1540
Size: 275 186 198 207 1015 761 1028 2301
Fragments arranged by size:
2301 1028 1015 761 275 207 198 186
BsiHKAI G_wGCw'C
Cuts at: 2174 2561 3153 4032 4117 5188 2174
Size: 387 592 879 85 1071 2957
Fragments arranged by size:
2957 1071 879 592 387 85
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
BsmBI CGTCTCn'nnnn_
Cuts at: 3152 3152
Size: 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2561 2719 3022 3153 3290 4032
Size: 399 384 387 158 303 131 137 742
Cuts at: 4032 4117 5188 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 384 303 158
137 131 85
BspEI T'CCGG_A
Cuts at: 2673 5634 2673
Size: 2961 3010
BspHI T'CATG_A
Cuts at: 2505 3860 5590 2505
Size: 1355 1730 2886
Fragments arranged by size:
2886 1730 1355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 844
Size: 2482 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2533 4466 4640 65
Size: 1183 1285 1933 174 1396
Fragments arranged by size:
1933 1396 1285 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 4615 4802 1700
Size: 110 388 2417 187 2869
Fragments arranged by size:
2869 2417 388 187 110
BsrGI T'GTAC_A
Cuts at: 96 3141 96
Size: 3045 2926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2067 2361 794
Size: 1273 294 4404
Fragments arranged by size:
4404 1273 294
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 2971 1184
Size: 1787 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2998 3106 2011
Size: 987 108 4876
Fragments arranged by size:
4876 987 108
BstYI r'GATC_y
Cuts at: 1972 2476 2901 3301 3668 4053 4070 5522 5608
Size: 504 425 400 367 385 17 1452 86
Cuts at: 5608 5620 5966 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 504 425 400 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2388 2574 2619 513
Size: 552 1323 186 45 3865
Fragments arranged by size:
3865 1323 552 186 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2971 487
Size: 683 56 1745 3487
Fragments arranged by size:
3487 1745 683 56
BtsI GCAGTG_nn'
Cuts at: 2790 3487 4281 4301 2790
Size: 697 794 20 4460
Fragments arranged by size:
4460 794 697 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3049 4978 809
Size: 507 1733 1929 1802
Fragments arranged by size:
1929 1802 1733 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2622 2640
Size: 54 1425 50 275 18 741 51 18
Cuts at: 2640 2688 3096 3418 4307 5936 8
Size: 48 408 322 889 1629 43
Fragments arranged by size:
1629 1425 889 741 408 322 275 54
51 50 48 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2665 3901 1353
Size: 1312 1236 3423
Fragments arranged by size:
3423 1312 1236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2172 2172
Size: 5971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 4034 4631 5418 5686 1239
Size: 393 526 1876 597 787 268 1524
Fragments arranged by size:
1876 1524 787 597 526 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2716 2716
Size: 5971
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2108 2108
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 2137 5118 1678
Size: 459 2981 2531
Fragments arranged by size:
2981 2531 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4612 4644 4686 4718 1373
Size: 32 135 32 3040 32 42 32 2626
Fragments arranged by size:
3040 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2624 2642 2690 10
Size: 54 1425 343 792 18 48 3291
Fragments arranged by size:
3291 1425 792 343 54 48 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2336 2573 2696 2741 3929 4288
Size: 648 843 327 237 123 45 1188 359
Cuts at: 4288 4447 518
Size: 159 2042
Fragments arranged by size:
2042 1188 843 648 359 327 237 159
123 45
MspA1I CmG'CkG
Cuts at: 1606 2064 3040 3123 3387 4064 5212 5457 1606
Size: 458 976 83 264 677 1148 245 2120
Fragments arranged by size:
2120 1148 976 677 458 264 245 83
NcoI C'CATG_G
Cuts at: 513 1065 2574 2619 513
Size: 552 1509 45 3865
Fragments arranged by size:
3865 1509 552 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2500 3329 4874 1132
Size: 97 1271 829 1545 2229
Fragments arranged by size:
2229 1545 1271 829 97
PasI CC'CwG_GG
Cuts at: 1348 3202 1348
Size: 1854 4117
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2521 1108
Size: 1413 4558
PfoI T'CCnGG_A
Cuts at: 2116 2116
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PspOMI G'GGCC_C
Cuts at: 2715 2715
Size: 5971
PstI C_TGCA'G
Cuts at: 830 2910 3199 3323 830
Size: 2080 289 124 3478
Fragments arranged by size:
3478 2080 289 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 3040 3123 3040
Size: 83 5888
SacI G_AGCT'C
Cuts at: 2174 2174
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 2906 3035 3195 3319 4454 5135 5326
Size: 219 1861 129 160 124 1135 681 191
Cuts at: 5326 826
Size: 1471
Fragments arranged by size:
1861 1471 1135 681 219 191 160 129
124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4075 4976 5238 820
Size: 197 926 2132 901 262 1553
Fragments arranged by size:
2132 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 2500 3329 1132
Size: 1368 829 3774
Fragments arranged by size:
3774 1368 829
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 2574 2619 3410 513
Size: 552 1509 45 791 3074
Fragments arranged by size:
3074 1509 791 552 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2149 3998 4015 4168 4353 4798 325
Size: 1255 569 1849 17 153 185 445 1498
Fragments arranged by size:
1849 1498 1255 569 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3141 4217 5929
Size: 274 80 33 51 1443 1164 1076 1712
Cuts at: 5929 96
Size: 138
Fragments arranged by size:
1712 1443 1164 1076 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2540 2572 2769 2801 5931 5963 2540
Size: 32 197 32 3130 32 2548
Fragments arranged by size:
3130 2548 197 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 2365
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2919 3779 276
Size: 53 83 186 936 1385 860 2468
Fragments arranged by size:
2468 1385 936 860 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI