Restriction map for
pFN21AB5184
(Circular) (Six-base) MAPSORT of: pFN21AB5184.seq Check: 8098 from: 1 to: 6523
pFN21AB5184 6523 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2248 2248
Size: 6523
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4333 278
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2258 3866 1937
Size: 321 1608 4594
Fragments arranged by size:
4594 1608 321
Acc65I G'GTAC_C
Cuts at: 3844 3844
Size: 6523
AclI AA'CG_TT
Cuts at: 1563 4650 5023 1563
Size: 3087 373 3063
Fragments arranged by size:
3087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 2073 2829 2942 3498 3694 4586 5970
Size: 411 30 756 113 556 196 892 1384
Cuts at: 5970 6238 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 892 756 556 411 268 196
113 30
AfeI AGC'GCT
Cuts at: 2909 2909
Size: 6523
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6326
AflIII A'CryG_T
Cuts at: 1203 5422 6399 1203
Size: 4219 977 1327
Fragments arranged by size:
4219 1327 977
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 5252 5252
Size: 6523
AleI CACnn'nnGTG
Cuts at: 1166 2948 1166
Size: 1782 4741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6523
AlwNI CAG_nnn'CTG
Cuts at: 2092 2287 4266 5838 2092
Size: 195 1979 1572 2777
Fragments arranged by size:
2777 1979 1572 195
ApaI G_GGCC'C
Cuts at: 2683 2719 3549 2683
Size: 36 830 5657
Fragments arranged by size:
5657 830 36
ApaLI G'TGCA_C
Cuts at: 4580 5736 4580
Size: 1156 5367
ApoI r'AATT_y
Cuts at: 1493 3832 4058 4210 6319 1493
Size: 2339 226 152 2109 1697
Fragments arranged by size:
2339 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5077 160
Size: 872 4045 1606
Fragments arranged by size:
4045 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 2229 2823 3848 1870
Size: 73 286 594 1025 4545
Fragments arranged by size:
4545 1025 594 286 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2268 2301 2388 2421 2268
Size: 33 87 33 6370
Fragments arranged by size:
6370 87 33 33
BamHI G'GATC_C
Cuts at: 3403 3594 3853 4220 3403
Size: 191 259 367 5706
Fragments arranged by size:
5706 367 259 191
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 3653 3719 3776 3800
Size: 325 217 627 449 1417 66 57 24
Cuts at: 3800 3844 5299 618
Size: 44 1455 1842
Fragments arranged by size:
1842 1455 1417 627 449 325 217 66
57 44 24
BanII G_rGCy'C
Cuts at: 1391 2683 2719 3066 3508 3525 3549 3753 3842
Size: 1292 36 347 442 17 24 204 89
Cuts at: 3842 1391
Size: 4072
Fragments arranged by size:
4072 1292 442 347 204 89 36 24
17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5552
BbvCI CC'TCA_GC
Cuts at: 3465 3465
Size: 6523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2009 2043 3505 3539 4714 4748 1903
Size: 34 72 34 1462 34 1175 34 3678
Fragments arranged by size:
3678 1462 1175 72 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2497 3207 4415 5625 2497
Size: 710 1208 1210 3395
Fragments arranged by size:
3395 1210 1208 710
BclI T'GATC_A
Cuts at: 1548 2578 1548
Size: 1030 5493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2144 2249 2642
Size: 107 122 71 631 605 472 105 393
Cuts at: 2642 2651 2678 2687 5134 136
Size: 9 27 9 2447 1525
Fragments arranged by size:
2447 1525 631 605 472 393 122 107
105 71 27 9 9
BglII A'GATC_T
Cuts at: 6518 6518
Size: 6523
BlpI GC'TnA_GC
Cuts at: 3940 3940
Size: 6523
Bme1580I G_kGCm'C
Cuts at: 1790 2683 2719 3549 4584 5740 1790
Size: 893 36 830 1035 1156 2573
Fragments arranged by size:
2573 1156 1035 893 830 36
BmgBI CAC'GTC
Cuts at: 1318 3238 1318
Size: 1920 4603
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2479 3092 3565
Size: 416 53 83 399 24 1062 613 473
Cuts at: 3565 4254 5212 442
Size: 689 958 1753
Fragments arranged by size:
1753 1062 958 689 613 473 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2747
Size: 6523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2774 5183 1239
Size: 932 603 2409 2579
Fragments arranged by size:
2579 2409 932 603
Bpu10I CC'TnA_GC
Cuts at: 2418 2463 3465 2418
Size: 45 1002 5476
Fragments arranged by size:
5476 1002 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3359 4648 5513 5811 3359
Size: 1289 865 298 4071
Fragments arranged by size:
4071 1289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2161 2179 2197 2215 2363 2623 3347
Size: 617 662 18 18 18 148 260 724
Cuts at: 3347 5186 882
Size: 1839 2219
Fragments arranged by size:
2219 1839 724 662 617 260 148 18
18 18
BsaAI yAC'GTr
Cuts at: 493 1204 4314 493
Size: 711 3110 2702
Fragments arranged by size:
3110 2702 711
BsaBI GATnn'nnATC
Cuts at: 2900 6517 2900
Size: 3617 2906
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4330 4712 5347 275
Size: 53 83 186 936 2797 382 635 1451
Fragments arranged by size:
2797 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4956 5354 5628 5775 6186 4956
Size: 398 274 147 411 5293
Fragments arranged by size:
5293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6298
Fragments arranged by size:
6298 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6523
BseYI C'CCAG_C
Cuts at: 2449 3109 5726 6222 2449
Size: 660 2617 496 2750
Fragments arranged by size:
2750 2617 660 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2049 2418 2496 1163
Size: 573 313 369 78 5190
Fragments arranged by size:
5190 573 369 313 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3973 4734 5762 1540
Size: 275 186 1972 761 1028 2301
Fragments arranged by size:
2301 1972 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3508 4584 4669 5740 3508
Size: 1076 85 1071 4291
Fragments arranged by size:
4291 1076 1071 85
BsmI GAATG_Cn'
Cuts at: 2338 3541 4034 4127 2338
Size: 1203 493 93 4734
Fragments arranged by size:
4734 1203 493 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2683 2719 3066 3508 3525 3549 3753
Size: 399 893 36 347 442 17 24 204
Cuts at: 3753 3842 4584 4669 5740 1391
Size: 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 893 742 442 399 347 204
89 85 36 24 17
BspEI T'CCGG_A
Cuts at: 6186 6186
Size: 6523
BspHI T'CATG_A
Cuts at: 3144 4412 6142 3144
Size: 1268 1730 3525
Fragments arranged by size:
3525 1730 1268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 2248 3878 844
Size: 1177 227 1630 3489
Fragments arranged by size:
3489 1630 1177 227
BsrBI CCG'CTC
Cuts at: 1394 1823 4410 1394
Size: 429 2587 3507
Fragments arranged by size:
3507 2587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5018 5192 65
Size: 1183 3770 174 1396
Fragments arranged by size:
3770 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5167 5354 1700
Size: 110 3357 187 2869
Fragments arranged by size:
3357 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3039 96
Size: 2943 3580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6523
BssSI C'ACGA_G
Cuts at: 4583 5595 4583
Size: 1012 5511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6523
BstBI TT'CG_AA
Cuts at: 5327 5327
Size: 6523
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6523
BstXI CCAn_nnnn'nTGG
Cuts at: 3635 3635
Size: 6523
BstYI r'GATC_y
Cuts at: 1972 3403 3594 3853 4220 4605 4622 6074 6160
Size: 1431 191 259 367 385 17 1452 86
Cuts at: 6160 6172 6518 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1431 385 367 346 259 191
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5971
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3452 487
Size: 683 56 2226 3558
Fragments arranged by size:
3558 2226 683 56
BtsI GCAGTG_nn'
Cuts at: 2581 3646 4039 4833 4853 2581
Size: 1065 393 794 20 4251
Fragments arranged by size:
4251 1065 794 393 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6488
DraI TTT'AAA
Cuts at: 3827 3827
Size: 6523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5530 809
Size: 507 4214 1802
Fragments arranged by size:
4214 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2608 3970 4859
Size: 54 1425 50 275 18 778 1362 889
Cuts at: 4859 6488 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1362 889 778 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3970 1537
Size: 275 2158 4090
Fragments arranged by size:
4090 2158 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2556 3758 4453 1353
Size: 1203 1202 695 3423
Fragments arranged by size:
3423 1203 1202 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5110 5484 5630 387
Size: 1064 138 3521 374 146 1280
Fragments arranged by size:
3521 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3506 3506
Size: 6523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2073 2171 2774 2829 2942 3498
Size: 393 411 30 98 603 55 113 556
Cuts at: 3498 3694 4586 5183 5970 6238 1239
Size: 196 892 597 787 268 1524
Fragments arranged by size:
1524 892 787 603 597 556 411 393
268 196 113 98 55 30
EcoO109I rG'GnC_Cy
Cuts at: 2119 2446 2663 3334 3546 2119
Size: 327 217 671 212 5096
Fragments arranged by size:
5096 671 327 217 212
EcoRI G'AATT_C
Cuts at: 3832 4210 3832
Size: 378 6145
EcoRV GAT'ATC
Cuts at: 3571 3571
Size: 6523
FspI TGC'GCA
Cuts at: 3191 5029 3191
Size: 1838 4685
HaeII r_GCGC'y
Cuts at: 1678 2911 5670 1678
Size: 1233 2759 2531
Fragments arranged by size:
2759 2531 1233
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2074 2092 2106 3490
Size: 32 135 32 488 14 18 14 1384
Cuts at: 3490 3522 3721 3753 5164 5196 5238 5270 1373
Size: 32 199 32 1411 32 42 32 2626
Fragments arranged by size:
2626 1411 1384 488 199 135 42 32
32 32 32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2097 3867 4113 669
Size: 988 281 159 1770 246 3079
Fragments arranged by size:
3079 1770 988 281 246 159
HindIII A'AGCT_T
Cuts at: 748 2055 748
Size: 1307 5216
HpaI GTT'AAC
Cuts at: 4113 4113
Size: 6523
KpnI G_GTAC'C
Cuts at: 3848 3848
Size: 6523
MfeI C'AATT_G
Cuts at: 3008 4122 3008
Size: 1114 5409
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5637 5821 1305
Size: 4332 184 2007
Fragments arranged by size:
4332 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4701
Fragments arranged by size:
4701 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2553 2948 3143 4481 4840 4999 518
Size: 648 1387 395 195 1338 359 159 2042
Fragments arranged by size:
2042 1387 1338 648 395 359 195 159
MspA1I CmG'CkG
Cuts at: 1606 2269 2294 2315 2480 3464 3476 3939 4616
Size: 663 25 21 165 984 12 463 677
Cuts at: 4616 5764 6009 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 984 677 663 463 245 165
25 21 12
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5971
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 2920 3881 5426 1132
Size: 97 1691 961 1545 2229
Fragments arranged by size:
2229 1691 1545 961 97
PasI CC'CwG_GG
Cuts at: 1348 3209 3579 1348
Size: 1861 370 4292
Fragments arranged by size:
4292 1861 370
PciI A'CATG_T
Cuts at: 5422 5422
Size: 6523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 2287 3414 1108
Size: 1005 174 1127 4217
Fragments arranged by size:
4217 1127 1005 174
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4592 4624 6105 6137 4592
Size: 32 1481 32 4978
Fragments arranged by size:
4978 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2663 2119
Size: 544 5979
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6523
PsiI TTA'TAA
Cuts at: 4093 4093
Size: 6523
PspOMI G'GGCC_C
Cuts at: 2679 2715 3545 2679
Size: 36 830 5657
Fragments arranged by size:
5657 830 36
PstI C_TGCA'G
Cuts at: 830 3463 3475 3875 830
Size: 2633 12 400 3478
Fragments arranged by size:
3478 2633 400 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
PvuII CAG'CTG
Cuts at: 2269 2294 2315 2480 3464 3476 2269
Size: 25 21 165 984 12 5316
Fragments arranged by size:
5316 984 165 25 21 12
SacI G_AGCT'C
Cuts at: 3508 3508
Size: 6523
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SanDI GG'GwC_CC
Cuts at: 2663 2663
Size: 6523
SapI GCTCTTCn'nnn_
Cuts at: 3758 3758
Size: 6523
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 3661 4771 6483 3661
Size: 1110 1712 3701
Fragments arranged by size:
3701 1712 1110
SfcI C'TryA_G
Cuts at: 826 1045 3459 3471 3871 5006 5687 5878 826
Size: 219 2414 12 400 1135 681 191 1471
Fragments arranged by size:
2414 1471 1135 681 400 219 191 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2642 2642
Size: 6523
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 3850 1872
Size: 1978 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3374 4627 5528 5790 820
Size: 197 926 1431 1253 901 262 1553
Fragments arranged by size:
1553 1431 1253 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4314 493
Size: 3821 2702
SpeI A'CTAG_T
Cuts at: 152 3662 152
Size: 3510 3013
SphI G_CATG'C
Cuts at: 1132 3881 1132
Size: 2749 3774
SspI AAT'ATT
Cuts at: 5 52 4447 5
Size: 47 4395 2081
Fragments arranged by size:
4395 2081 47
StuI AGG'CCT
Cuts at: 1517 2138 2252 1517
Size: 621 114 5788
Fragments arranged by size:
5788 621 114
StyI C'CwwG_G
Cuts at: 513 1065 2514 3408 3483 3763 3962 513
Size: 552 1449 894 75 280 199 3074
Fragments arranged by size:
3074 1449 894 552 280 199 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 3118 3147 4550 4567 4720 4905
Size: 1255 681 857 29 1403 17 153 185
Cuts at: 4905 5350 325
Size: 445 1498
Fragments arranged by size:
1498 1403 1255 857 681 445 185 153
29 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3039 3659 4769
Size: 274 80 33 51 1443 1062 620 1110
Cuts at: 4769 6481 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1110 1062 620 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6483 6515 6483
Size: 32 6491
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6523
XbaI T'CTAG_A
Cuts at: 3859 3859
Size: 6523
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6523
XmaI C'CCGG_G
Cuts at: 1870 3848 1870
Size: 1978 4545
XmnI GAAnn'nnTTC
Cuts at: 4652 4652
Size: 6523
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4331 276
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SexAI SfoI SrfI SwaI XcmI