Restriction map for
pFN21AB5183
(Circular) (Six-base) MAPSORT of: pFN21AB5183.seq Check: 2422 from: 1 to: 6007
pFN21AB5183 6007 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2568 3350 1937
Size: 631 782 4594
Fragments arranged by size:
4594 782 631
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 3078 4070 5454 5722 1632
Size: 581 865 992 1384 268 1917
Fragments arranged by size:
1917 1384 992 865 581 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 4906 5883 1203
Size: 3703 977 1327
Fragments arranged by size:
3703 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 2788 4736 2788
Size: 1948 4059
AleI CACnn'nnGTG
Cuts at: 1166 2621 1166
Size: 1455 4552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3038 1184
Size: 1854 4153
AlwNI CAG_nnn'CTG
Cuts at: 3750 5322 3750
Size: 1572 4435
ApaI G_GGCC'C
Cuts at: 2029 2029
Size: 6007
ApaLI G'TGCA_C
Cuts at: 3169 4064 5220 3169
Size: 895 1156 3956
Fragments arranged by size:
3956 1156 895
ApoI r'AATT_y
Cuts at: 1493 3316 3542 3694 5803 1493
Size: 1823 226 152 2109 1697
Fragments arranged by size:
2109 1823 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 3332 1870
Size: 73 85 1304 4545
Fragments arranged by size:
4545 1304 85 73
BamHI G'GATC_C
Cuts at: 2940 3337 3704 2940
Size: 397 367 5243
Fragments arranged by size:
5243 397 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3191 3328 4783 618
Size: 325 217 627 1404 137 1455 1842
Fragments arranged by size:
1842 1455 1404 627 325 217 137
BanII G_rGCy'C
Cuts at: 1391 2029 3326 1391
Size: 638 1297 4072
Fragments arranged by size:
4072 1297 638
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5036
BbvCI CC'TCA_GC
Cuts at: 2150 2150
Size: 6007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4198 4232 1903
Size: 34 2261 34 3678
Fragments arranged by size:
3678 2261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 2460 1548
Size: 912 5095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2019 6002 2019
Size: 3983 2024
BlpI GC'TnA_GC
Cuts at: 2376 2409 3424 2376
Size: 33 1015 4959
Fragments arranged by size:
4959 1015 33
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2112 2839 3173 3196 4068 5224 1790
Size: 239 83 727 334 23 872 1156 2573
Fragments arranged by size:
2573 1156 872 727 334 239 83 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2106 2513 2829
Size: 416 53 83 399 24 689 407 316
Cuts at: 2829 3291 3738 4696 442
Size: 462 447 958 1753
Fragments arranged by size:
1753 958 689 462 447 416 407 399
316 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2309 2678 4667 1239
Size: 926 144 369 1989 2579
Fragments arranged by size:
2579 1989 926 369 144
Bpu10I CC'TnA_GC
Cuts at: 2150 2242 3121 2150
Size: 92 879 5036
Fragments arranged by size:
5036 879 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 3014 4132 4997 5295 2564
Size: 450 1118 865 298 3276
Fragments arranged by size:
3276 1118 865 450 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2252 2878 4670 882
Size: 617 753 626 1792 2219
Fragments arranged by size:
2219 1792 753 626 617
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3814 4196 4831 275
Size: 53 83 186 936 2281 382 635 1451
Fragments arranged by size:
2281 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 1178
Size: 30 165 30 795 30 4957
Fragments arranged by size:
4957 795 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6007
BseYI C'CCAG_C
Cuts at: 2830 3096 3253 5210 5706 2830
Size: 266 157 1957 496 3131
Fragments arranged by size:
3131 1957 496 266 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2580 1163
Size: 573 844 4590
Fragments arranged by size:
4590 844 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3173 4068 4153 5224 3173
Size: 895 85 1071 3956
Fragments arranged by size:
3956 1071 895 85
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2112 2839 3173 3196 3326 4068
Size: 399 239 83 727 334 23 130 742
Cuts at: 4068 4153 5224 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 727 399 334 239 130
85 83 23
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 2508 3234 3896 5626 2508
Size: 726 662 1730 2889
Fragments arranged by size:
2889 1730 726 662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3362 844
Size: 2518 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2380 2992 3894 1394
Size: 429 557 612 902 3507
Fragments arranged by size:
3507 902 612 557 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2403 3144 4502 4676 65
Size: 1183 1155 741 1358 174 1396
Fragments arranged by size:
1396 1358 1183 1155 741 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2446 4651 4838 1700
Size: 110 636 2205 187 2869
Fragments arranged by size:
2869 2205 636 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6007
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 2656 3092 3304 2623
Size: 33 436 212 5326
Fragments arranged by size:
5326 436 212 33
BstYI r'GATC_y
Cuts at: 1972 2019 2034 2071 2940 3337 3704 4089 4106
Size: 47 15 37 869 397 367 385 17
Cuts at: 4106 5558 5644 5656 6002 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 869 397 385 367 346 86
47 37 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2047 513
Size: 552 982 4473
Fragments arranged by size:
4473 982 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2206 3101 487
Size: 683 56 980 895 3393
Fragments arranged by size:
3393 980 895 683 56
BtsI GCAGTG_nn'
Cuts at: 2112 2205 3003 3523 4317 4337 2112
Size: 93 798 520 794 20 3782
Fragments arranged by size:
3782 798 794 520 93 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 2415 2493
Size: 54 1425 50 275 18 522 63 78
Cuts at: 2493 3454 4343 5972 8
Size: 961 889 1629 43
Fragments arranged by size:
1629 1425 961 889 522 275 78 63
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3243 3937 1353
Size: 1890 694 3423
Fragments arranged by size:
3423 1890 694
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2213 2309 2678 3078 4070 4667
Size: 393 533 48 96 369 400 992 597
Cuts at: 4667 5454 5722 1239
Size: 787 268 1524
Fragments arranged by size:
1524 992 787 597 533 400 393 369
268 96 48
EcoO109I rG'GnC_Cy
Cuts at: 2140 2884 2140
Size: 744 5263
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 5154 1678
Size: 3476 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 2774 2806 4648
Size: 32 135 32 725 32 445 32 1842
Cuts at: 4648 4680 4722 4754 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1842 725 445 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3037 3351 3597 669
Size: 988 281 1099 314 246 3079
Fragments arranged by size:
3079 1099 988 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 5121 5305 1305
Size: 969 2847 184 2007
Fragments arranged by size:
2847 2007 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2495 10
Size: 54 1425 343 663 3522
Fragments arranged by size:
3522 1425 663 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 3965 4324 4483 518
Size: 648 1455 1344 359 159 2042
Fragments arranged by size:
2042 1455 1344 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2375 2413 2830 3423 4100 5248 5493
Size: 443 326 38 417 593 677 1148 245
Cuts at: 5493 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 593 443 417 326 245
38
NaeI GCC'GGC
Cuts at: 2448 2448
Size: 6007
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5455
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NgoMIV G'CCGG_C
Cuts at: 2446 2446
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 3365 4910 1132
Size: 97 2136 1545 2229
Fragments arranged by size:
2229 2136 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2950 1108
Size: 1842 4165
PfoI T'CCnGG_A
Cuts at: 2037 2655 2037
Size: 618 5389
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2884 2884
Size: 6007
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PspOMI G'GGCC_C
Cuts at: 2025 2025
Size: 6007
PstI C_TGCA'G
Cuts at: 830 2107 3359 830
Size: 1277 1252 3478
Fragments arranged by size:
3478 1277 1252
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2375 2413 2830 2375
Size: 38 417 5552
Fragments arranged by size:
5552 417 38
SacII CC_GC'GG
Cuts at: 2050 2050
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SapI GCTCTTCn'nnn_
Cuts at: 3243 3243
Size: 6007
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SexAI A'CCwGG_T
Cuts at: 3153 3153
Size: 6007
SfcI C'TryA_G
Cuts at: 826 1045 2103 3355 4490 5171 5362 826
Size: 219 1058 1252 1135 681 191 1471
Fragments arranged by size:
1471 1252 1135 1058 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 2030 3334 1872
Size: 158 1304 4545
Fragments arranged by size:
4545 1304 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 3029 4111 5012 5274 820
Size: 197 926 636 450 1082 901 262 1553
Fragments arranged by size:
1553 1082 926 901 636 450 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 3011 1517
Size: 1494 4513
StyI C'CwwG_G
Cuts at: 513 1065 2673 3446 513
Size: 552 1608 773 3074
Fragments arranged by size:
3074 1608 773 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 2942 3040 4034 4051 4204 4389
Size: 1255 750 612 98 994 17 153 185
Cuts at: 4389 4834 325
Size: 445 1498
Fragments arranged by size:
1498 1255 994 750 612 445 185 153
98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4253 5965 96
Size: 274 80 33 51 1443 2276 1712 138
Fragments arranged by size:
2276 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 5967 5999 2198
Size: 32 3737 32 2206
Fragments arranged by size:
3737 2206 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2852 1316
Size: 1536 4471
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2335 3185 2065
Size: 270 850 4887
Fragments arranged by size:
4887 850 270
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 2028 3332 1870
Size: 158 1304 4545
Fragments arranged by size:
4545 1304 158
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SanDI SfiI
SfoI SrfI SwaI