Restriction map for
pFN21AB5181
(Circular) (Six-base) MAPSORT of: pFN21AB5181.seq Check: 9035 from: 1 to: 6673
pFN21AB5181 6673 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4483 278
Size: 53 83 186 936 2947 2468
Fragments arranged by size:
2947 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4016 1937
Size: 2079 4594
Acc65I G'GTAC_C
Cuts at: 3191 3994 3191
Size: 803 5870
AclI AA'CG_TT
Cuts at: 1563 4800 5173 1563
Size: 3237 373 3063
Fragments arranged by size:
3237 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 3156 4736 6120 6388 1632
Size: 989 535 1580 1384 268 1917
Fragments arranged by size:
1917 1580 1384 989 535 268
AfeI AGC'GCT
Cuts at: 2819 2819
Size: 6673
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6476
AflIII A'CryG_T
Cuts at: 1203 5572 6549 1203
Size: 4369 977 1327
Fragments arranged by size:
4369 1327 977
AgeI A'CCGG_T
Cuts at: 5504 5504
Size: 6673
AhdI GACnn_n'nnGTC
Cuts at: 5402 5402
Size: 6673
AleI CACnn'nnGTG
Cuts at: 1166 2182 2258 2921 1166
Size: 1016 76 663 4918
Fragments arranged by size:
4918 1016 663 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3123 3575 3608 3809 1184
Size: 1939 452 33 201 4048
Fragments arranged by size:
4048 1939 452 201 33
AlwNI CAG_nnn'CTG
Cuts at: 2780 3242 3358 3447 3841 4416 5988 2780
Size: 462 116 89 394 575 1572 3465
Fragments arranged by size:
3465 1572 575 462 394 116 89
ApaLI G'TGCA_C
Cuts at: 4730 5886 4730
Size: 1156 5517
ApoI r'AATT_y
Cuts at: 1493 3982 4208 4360 6469 1493
Size: 2489 226 152 2109 1697
Fragments arranged by size:
2489 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5227 160
Size: 872 4195 1606
Fragments arranged by size:
4195 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6673
AvaI C'yCGr_G
Cuts at: 1870 1943 2435 3645 3781 3998 1870
Size: 73 492 1210 136 217 4545
Fragments arranged by size:
4545 1210 492 217 136 73
BamHI G'GATC_C
Cuts at: 4003 4370 4003
Size: 367 6306
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3047 3191 3994 5449 618
Size: 325 217 627 1260 144 803 1455 1842
Fragments arranged by size:
1842 1455 1260 803 627 325 217 144
BanII G_rGCy'C
Cuts at: 1391 2013 2079 2337 2369 2409 2609 3138 3234
Size: 622 66 258 32 40 200 529 96
Cuts at: 3234 3504 3540 3992 1391
Size: 270 36 452 4072
Fragments arranged by size:
4072 622 529 452 270 258 200 96
66 40 36 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2625 3344 928
Size: 971 726 719 4257
Fragments arranged by size:
4257 971 726 719
BbvCI CC'TCA_GC
Cuts at: 3823 3823
Size: 6673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4864 4898 1903
Size: 34 2927 34 3678
Fragments arranged by size:
3678 2927 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4565 5775 4565
Size: 1210 5463
BclI T'GATC_A
Cuts at: 1548 2925 1548
Size: 1377 5296
BfrBI ATG'CAT
Cuts at: 2274 2274
Size: 6673
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2769 3508 5284
Size: 107 122 71 631 605 1097 739 1776
Cuts at: 5284 136
Size: 1525
Fragments arranged by size:
1776 1525 1097 739 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2397 2685 6668 2397
Size: 288 3983 2402
Fragments arranged by size:
3983 2402 288
BlpI GC'TnA_GC
Cuts at: 3698 4090 3698
Size: 392 6281
Bme1580I G_kGCm'C
Cuts at: 1790 3050 3564 3788 4734 5890 1790
Size: 1260 514 224 946 1156 2573
Fragments arranged by size:
2573 1260 1156 946 514 224
BmgBI CAC'GTC
Cuts at: 1318 3673 1318
Size: 2355 4318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2092 3099 3255
Size: 416 53 83 399 24 675 1007 156
Cuts at: 3255 4404 5362 442
Size: 1149 958 1753
Fragments arranged by size:
1753 1149 1007 958 675 416 399 156
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6673
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 6673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 3448 3587 5333 1239
Size: 815 1394 139 1746 2579
Fragments arranged by size:
2579 1746 1394 815 139
Bpu10I CC'TnA_GC
Cuts at: 2884 3096 3823 2884
Size: 212 727 5734
Fragments arranged by size:
5734 727 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4798 5663 5961 4798
Size: 865 298 5510
Fragments arranged by size:
5510 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5336 882
Size: 617 3837 2219
Fragments arranged by size:
3837 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4464 493
Size: 711 3260 2702
Fragments arranged by size:
3260 2702 711
BsaBI GATnn'nnATC
Cuts at: 6667 6667
Size: 6673
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4480 4862 5497 275
Size: 53 83 186 936 2947 382 635 1451
Fragments arranged by size:
2947 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3194 5106 5504 5778 5925 6336 3194
Size: 1912 398 274 147 411 3531
Fragments arranged by size:
3531 1912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6448
Fragments arranged by size:
6448 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2453 2526 2585 2726 2972 1186
Size: 1213 54 73 59 141 246 4887
Fragments arranged by size:
4887 1213 246 141 73 59 54
BseYI C'CCAG_C
Cuts at: 3058 3235 3280 3372 3458 3581 3711 5876 6372
Size: 177 45 92 86 123 130 2165 496
Cuts at: 6372 3058
Size: 3359
Fragments arranged by size:
3359 2165 496 177 130 123 92 86
45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2678 1163
Size: 573 942 5158
Fragments arranged by size:
5158 942 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2546 4123 4884 5912 1540
Size: 275 186 545 1577 761 1028 2301
Fragments arranged by size:
2301 1577 1028 761 545 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2337 2409 2609 3002 3138 3540 3704 4734
Size: 258 72 200 393 136 402 164 1030
Cuts at: 4734 4819 5890 2079
Size: 85 1071 2862
Fragments arranged by size:
2862 1071 1030 402 393 258 200 164
136 85 72
BsmI GAATG_Cn'
Cuts at: 2707 4184 4277 2707
Size: 1477 93 5103
Fragments arranged by size:
5103 1477 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2079 2337 2369 2409 2609 3002
Size: 399 223 66 258 32 40 200 393
Cuts at: 3002 3050 3138 3234 3504 3540 3564 3704 3788
Size: 48 88 96 270 36 24 140 84
Cuts at: 3788 3992 4734 4819 5890 1391
Size: 204 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 270 258 223
204 200 140 96 88 85 84 66
48 40 36 32 24
BspEI T'CCGG_A
Cuts at: 6336 6336
Size: 6673
BspHI T'CATG_A
Cuts at: 2799 4562 6292 2799
Size: 1763 1730 3180
Fragments arranged by size:
3180 1763 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3435 4028 844
Size: 2591 593 3489
Fragments arranged by size:
3489 2591 593
BsrBI CCG'CTC
Cuts at: 1394 1823 2827 4560 1394
Size: 429 1004 1733 3507
Fragments arranged by size:
3507 1733 1004 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5168 5342 65
Size: 1183 3920 174 1396
Fragments arranged by size:
3920 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2981 5317 5504 1700
Size: 110 1171 2336 187 2869
Fragments arranged by size:
2869 2336 1171 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6673
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6673
BssSI C'ACGA_G
Cuts at: 4733 5745 4733
Size: 1012 5661
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2947 3358 3447 794
Size: 2153 411 89 4020
Fragments arranged by size:
4020 2153 411 89
BstBI TT'CG_AA
Cuts at: 5477 5477
Size: 6673
BstEII G'GTnAC_C
Cuts at: 1184 3905 1184
Size: 2721 3952
BstXI CCAn_nnnn'nTGG
Cuts at: 2184 2263 2362 2587 2184
Size: 79 99 225 6270
Fragments arranged by size:
6270 225 99 79
BstYI r'GATC_y
Cuts at: 1972 2166 2397 2685 2892 3016 3031 4003 4370
Size: 194 231 288 207 124 15 972 367
Cuts at: 4370 4755 4772 6224 6310 6322 6668 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 972 385 367 346 288 231
207 194 124 86 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2540 3900 513
Size: 552 939 536 1360 3286
Fragments arranged by size:
3286 1360 939 552 536
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2030 487
Size: 683 56 804 5130
Fragments arranged by size:
5130 804 683 56
BtsI GCAGTG_nn'
Cuts at: 3653 4189 4983 5003 3653
Size: 536 794 20 5323
Fragments arranged by size:
5323 794 536 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6673
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3365 3400 529
Size: 35 2801 35 3802
Fragments arranged by size:
3802 2801 35 35
DraI TTT'AAA
Cuts at: 3977 3977
Size: 6673
DraIII CAC_nnn'GTG
Cuts at: 3490 3490
Size: 6673
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5680 809
Size: 507 4364 1802
Fragments arranged by size:
4364 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2543 3546 4120
Size: 54 1425 50 275 18 713 1003 574
Cuts at: 4120 5009 6638 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1003 889 713 574 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2543 4120 1537
Size: 275 731 1577 4090
Fragments arranged by size:
4090 1577 731 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 2597 2687 2849 3638 4603 1353
Size: 920 324 90 162 789 965 3423
Fragments arranged by size:
3423 965 920 789 324 162 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5260 5634 5780 387
Size: 1064 138 3671 374 146 1280
Fragments arranged by size:
3671 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2077 2335 2407 2607 3136 3538 2077
Size: 258 72 200 529 402 5212
Fragments arranged by size:
5212 529 402 258 200 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2621 3156 3448 3587 4736 5333
Size: 393 422 567 535 292 139 1149 597
Cuts at: 5333 6120 6388 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1149 787 597 567 535 422 393
292 268 139
EcoNI CCTnn'n_nnAGG
Cuts at: 2069 2435 2801 2918 2069
Size: 366 366 117 5824
Fragments arranged by size:
5824 366 366 117
EcoO109I rG'GnC_Cy
Cuts at: 2132 2794 3256 3424 3649 3838 2132
Size: 662 462 168 225 189 4967
Fragments arranged by size:
4967 662 462 225 189 168
EcoRI G'AATT_C
Cuts at: 3982 4360 3982
Size: 378 6295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 3910 2338
Size: 1572 5101
FspI TGC'GCA
Cuts at: 5179 5179
Size: 6673
HaeII r_GCGC'y
Cuts at: 1678 2821 3471 3667 5820 1678
Size: 1143 650 196 2153 2531
Fragments arranged by size:
2531 2153 1143 650 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2561 2593 2596 2628 3125
Size: 32 135 32 989 32 3 32 497
Cuts at: 3125 3157 5314 5346 5388 5420 1373
Size: 32 2157 32 42 32 2626
Fragments arranged by size:
2626 2157 989 497 135 42 32 32
32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2965 4017 4263 669
Size: 988 281 1027 1052 246 3079
Fragments arranged by size:
3079 1052 1027 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2504 748
Size: 1756 4917
HpaI GTT'AAC
Cuts at: 4263 4263
Size: 6673
KpnI G_GTAC'C
Cuts at: 3195 3998 3195
Size: 803 5870
MfeI C'AATT_G
Cuts at: 4272 4272
Size: 6673
MluI A'CGCG_T
Cuts at: 6549 6549
Size: 6673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 2872 3198 3693 5787 5971 1305
Size: 1215 352 326 495 2094 184 2007
Fragments arranged by size:
2094 2007 1215 495 352 326 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3548 10
Size: 54 1425 343 1716 3135
Fragments arranged by size:
3135 1716 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2182 2258 2744 2921 3557 4631 4990
Size: 648 1016 76 486 177 636 1074 359
Cuts at: 4990 5149 518
Size: 159 2042
Fragments arranged by size:
2042 1074 1016 648 636 486 359 177
159 76
MspA1I CmG'CkG
Cuts at: 1606 2418 2780 2944 3309 3447 3743 4089 4766
Size: 812 362 164 365 138 296 346 677
Cuts at: 4766 5914 6159 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 812 677 365 362 346 296
245 164 138
NaeI GCC'GGC
Cuts at: 2983 2983
Size: 6673
NcoI C'CATG_G
Cuts at: 513 1065 2004 3900 513
Size: 552 939 1896 3286
Fragments arranged by size:
3286 1896 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6673
NgoMIV G'CCGG_C
Cuts at: 2981 2981
Size: 6673
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6673
NotI GC'GGCC_GC
Cuts at: 4120 4120
Size: 6673
NsiI A_TGCA'T
Cuts at: 2276 2276
Size: 6673
NspI r_CATG'y
Cuts at: 1132 1229 2274 4031 5576 1132
Size: 97 1045 1757 1545 2229
Fragments arranged by size:
2229 1757 1545 1045 97
PasI CC'CwG_GG
Cuts at: 1348 2993 1348
Size: 1645 5028
PciI A'CATG_T
Cuts at: 5572 5572
Size: 6673
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6673
PfoI T'CCnGG_A
Cuts at: 3420 3420
Size: 6673
PmeI GTTT'AAAC
Cuts at: 3977 3977
Size: 6673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4742 4774 6255 6287 4742
Size: 32 1481 32 5128
Fragments arranged by size:
5128 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 3424 3649 3838 2794
Size: 630 225 189 5629
Fragments arranged by size:
5629 630 225 189
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6673
PsiI TTA'TAA
Cuts at: 4243 4243
Size: 6673
PstI C_TGCA'G
Cuts at: 830 2901 3351 3432 3452 3774 4025 830
Size: 2071 450 81 20 322 251 3478
Fragments arranged by size:
3478 2071 450 322 251 81 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6673
PvuII CAG'CTG
Cuts at: 2418 2780 2944 3447 3743 2418
Size: 362 164 503 296 5348
Fragments arranged by size:
5348 503 362 296 164
SacI G_AGCT'C
Cuts at: 2079 2337 2409 2609 3138 3540 2079
Size: 258 72 200 529 402 5212
Fragments arranged by size:
5212 529 402 258 200 72
SalI G'TCGA_C
Cuts at: 1936 4015 1936
Size: 2079 4594
SanDI GG'GwC_CC
Cuts at: 3649 3649
Size: 6673
SapI GCTCTTCn'nnn_
Cuts at: 2273 2597 2849 2273
Size: 324 252 6097
Fragments arranged by size:
6097 324 252
SbfI CC_TGCA'GG
Cuts at: 2901 4025 2901
Size: 1124 5549
ScaI AGT'ACT
Cuts at: 4921 6633 4921
Size: 1712 4961
SfcI C'TryA_G
Cuts at: 826 1045 2413 2897 3347 3428 3448 3770 4021
Size: 219 1368 484 450 81 20 322 251
Cuts at: 4021 5156 5837 6028 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1368 1135 681 484 450 322 251
219 191 81 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6673
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6673
SmaI CCC'GGG
Cuts at: 1872 3647 4000 1872
Size: 1775 353 4545
Fragments arranged by size:
4545 1775 353
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4777 5678 5940 820
Size: 197 926 2834 901 262 1553
Fragments arranged by size:
2834 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4464 493
Size: 3971 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6673
SphI G_CATG'C
Cuts at: 1132 4031 1132
Size: 2899 3774
SspI AAT'ATT
Cuts at: 5 52 4597 5
Size: 47 4545 2081
Fragments arranged by size:
4545 2081 47
StuI AGG'CCT
Cuts at: 1517 2201 2459 2882 3027 3511 1517
Size: 684 258 423 145 484 4679
Fragments arranged by size:
4679 684 484 423 258 145
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 3750 3900 4112 513
Size: 552 939 252 1494 150 212 3074
Fragments arranged by size:
3074 1494 939 552 252 212 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2933 3848 3893 4700 4717 4870 5055
Size: 1255 1353 915 45 807 17 153 185
Cuts at: 5055 5500 325
Size: 445 1498
Fragments arranged by size:
1498 1353 1255 915 807 445 185 153
45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4919 6631 96
Size: 274 80 33 51 1443 2942 1712 138
Fragments arranged by size:
2942 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2218 2250 2509 2541 3476 3508 6633 6665 2218
Size: 32 259 32 935 32 3125 32 2226
Fragments arranged by size:
3125 2226 935 259 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6673
XbaI T'CTAG_A
Cuts at: 4009 4009
Size: 6673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 3957 2152
Size: 1805 4868
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6673
XmaI C'CCGG_G
Cuts at: 1870 3645 3998 1870
Size: 1775 353 4545
Fragments arranged by size:
4545 1775 353
XmnI GAAnn'nnTTC
Cuts at: 3206 4802 3206
Size: 1596 5077
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4481 276
Size: 53 83 186 936 2947 2468
Fragments arranged by size:
2947 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI