Restriction map for
pFN21AB5174
(Circular) (Six-base) MAPSORT of: pFN21AB5174.seq Check: 1856 from: 1 to: 5899
pFN21AB5174 5899 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3067 3962 5346 5614 1632
Size: 1435 895 1384 268 1917
Fragments arranged by size:
1917 1435 1384 895 268
AfeI AGC'GCT
Cuts at: 2514 3098 2514
Size: 584 5315
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 4798 5775 1203
Size: 3595 977 1327
Fragments arranged by size:
3595 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 3642 5214 3642
Size: 1572 4327
ApaI G_GGCC'C
Cuts at: 2430 2801 2430
Size: 371 5528
ApaLI G'TGCA_C
Cuts at: 2631 3153 3956 5112 2631
Size: 522 803 1156 3418
Fragments arranged by size:
3418 1156 803 522
ApoI r'AATT_y
Cuts at: 1493 3208 3434 3586 5695 1493
Size: 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 2498 2693 2718 2998 3064 3224 1870
Size: 73 555 195 25 280 66 160 4545
Fragments arranged by size:
4545 555 280 195 160 73 66 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3162 3195 3162
Size: 33 5866
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2102 2261 2676 3220
Size: 325 217 627 242 73 159 415 544
Cuts at: 3220 4675 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 544 415 325 242 217
159 73
BanII G_rGCy'C
Cuts at: 1391 2430 2505 2801 3218 1391
Size: 1039 75 296 417 4072
Fragments arranged by size:
4072 1039 417 296 75
BbeI G_GCGC'C
Cuts at: 2033 2265 2680 2033
Size: 232 415 5252
Fragments arranged by size:
5252 415 232
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2341 928
Size: 971 442 4486
Fragments arranged by size:
4486 971 442
BbvCI CC'TCA_GC
Cuts at: 3030 3030
Size: 5899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2689 2786 4510
Size: 107 122 71 631 605 1017 97 1724
Cuts at: 4510 136
Size: 1525
Fragments arranged by size:
1724 1525 1017 631 605 122 107 97
71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 2375 2762 3316 2375
Size: 387 554 4958
Fragments arranged by size:
4958 554 387
Bme1580I G_kGCm'C
Cuts at: 1790 2430 2635 2801 2837 3157 3960 5116 1790
Size: 640 205 166 36 320 803 1156 2573
Fragments arranged by size:
2573 1156 803 640 320 205 166 36
BmgBI CAC'GTC
Cuts at: 1318 2827 1318
Size: 1509 4390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3630 4588 442
Size: 416 53 83 399 24 2213 958 1753
Fragments arranged by size:
2213 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2425 4559 1239
Size: 1178 8 2134 2579
Fragments arranged by size:
2579 2134 1178 8
Bpu10I CC'TnA_GC
Cuts at: 3030 3030
Size: 5899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4024 4889 5187 4024
Size: 865 298 4736
Fragments arranged by size:
4736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2988 4562 882
Size: 617 1489 1574 2219
Fragments arranged by size:
2219 1574 1489 617
BsaAI yAC'GTr
Cuts at: 493 1204 2584 3690 493
Size: 711 1380 1106 2702
Fragments arranged by size:
2702 1380 1106 711
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2121 2191 2262
Size: 53 83 186 936 497 91 70 71
Cuts at: 2262 2346 2677 2977 3706 4088 4723 275
Size: 84 331 300 729 382 635 1451
Fragments arranged by size:
1451 936 729 635 497 382 331 300
186 91 84 83 71 70 53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 1178
Size: 30 165 30 1131 30 4513
Fragments arranged by size:
4513 1131 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2486 1186
Size: 1300 4599
BseYI C'CCAG_C
Cuts at: 2515 3009 3108 5102 5598 2515
Size: 494 99 1994 496 2816
Fragments arranged by size:
2816 1994 496 494 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5326
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2027 3349 4110 5138 1540
Size: 275 186 26 1322 761 1028 2301
Fragments arranged by size:
2301 1322 1028 761 275 186 26
BsiHKAI G_wGCw'C
Cuts at: 2187 2589 2601 2635 2775 3157 3960 4045 5116
Size: 402 12 34 140 382 803 85 1071
Cuts at: 5116 2187
Size: 2970
Fragments arranged by size:
2970 1071 803 402 382 140 85 34
12
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
BsmBI CGTCTCn'nnnn_
Cuts at: 2443 2443
Size: 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2430 2505 2589 2601 2635 2775
Size: 399 397 243 75 84 12 34 140
Cuts at: 2775 2801 2837 3157 3218 3960 4045 5116 1391
Size: 26 36 320 61 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 320 243 140
85 84 75 61 36 34 26 12
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2949 3254 844
Size: 2105 305 3489
Fragments arranged by size:
3489 2105 305
BsrBI CCG'CTC
Cuts at: 1394 1823 2299 2566 3786 1394
Size: 429 476 267 1220 3507
Fragments arranged by size:
3507 1220 476 429 267
BsrDI GCAATG_nn'
Cuts at: 65 1248 4394 4568 65
Size: 1183 3146 174 1396
Fragments arranged by size:
3146 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2026 2032 2069 2105 2683 3187 4543
Size: 110 216 6 37 36 578 504 1356
Cuts at: 4543 4730 1700
Size: 187 2869
Fragments arranged by size:
2869 1356 578 504 216 187 110 37
36 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 2087 2113 2417 2642 2840 1925
Size: 162 26 304 225 198 4984
Fragments arranged by size:
4984 304 225 198 162 26
BssSI C'ACGA_G
Cuts at: 3959 4971 3959
Size: 1012 4887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2363 2652 794
Size: 1569 289 4041
Fragments arranged by size:
4041 1569 289
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstXI CCAn_nnnn'nTGG
Cuts at: 2395 2395
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 2248 3229 3596 3981 3998 5450 5536 5548
Size: 276 981 367 385 17 1452 86 12
Cuts at: 5548 5894 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 981 385 367 346 276 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2080 2124 2412 2472 2889 513
Size: 552 939 76 44 288 60 417 3523
Fragments arranged by size:
3523 939 552 417 288 76 60 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2374 2477 3087 487
Size: 683 56 1148 103 610 3299
Fragments arranged by size:
3299 1148 683 610 103 56
BtsI GCAGTG_nn'
Cuts at: 2361 3415 4209 4229 2361
Size: 1054 794 20 4031
Fragments arranged by size:
4031 1054 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 2385 2619
Size: 54 1425 50 275 18 194 361 234
Cuts at: 2619 2886 2892 3346 4235 5864 8
Size: 267 6 454 889 1629 43
Fragments arranged by size:
1629 1425 889 454 361 275 267 234
194 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2024 3346 1537
Size: 275 212 1322 4090
Fragments arranged by size:
4090 1322 275 212
EarI CTCTTCn'nnn_
Cuts at: 1353 2594 2810 3829 1353
Size: 1241 216 1019 3423
Fragments arranged by size:
3423 1241 1019 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4486 4860 5006 387
Size: 1064 138 2897 374 146 1280
Fragments arranged by size:
2897 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2425 3067 3962 4559 5346 5614
Size: 393 785 8 642 895 597 787 268
Cuts at: 5614 1239
Size: 1524
Fragments arranged by size:
1524 895 787 785 642 597 393 268
8
EcoO109I rG'GnC_Cy
Cuts at: 2426 2797 2798 3043 2426
Size: 371 1 245 5282
Fragments arranged by size:
5282 371 245 1
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
FspI TGC'GCA
Cuts at: 2756 4405 2756
Size: 1649 4250
HaeII r_GCGC'y
Cuts at: 1678 2033 2265 2516 2680 3100 5046 1678
Size: 355 232 251 164 420 1946 2531
Fragments arranged by size:
2531 1946 420 355 251 232 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 3086 3118 4540
Size: 32 135 32 962 32 520 32 1422
Cuts at: 4540 4572 4614 4646 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1422 962 520 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3243 3489 669
Size: 988 281 1305 246 3079
Fragments arranged by size:
3079 1305 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KasI G'GCGC_C
Cuts at: 2029 2261 2676 2029
Size: 232 415 5252
Fragments arranged by size:
5252 415 232
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 5013 5197 1305
Size: 921 2787 184 2007
Fragments arranged by size:
2787 2007 921 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 2888 2894 10
Size: 54 1425 343 555 501 6 3015
Fragments arranged by size:
3015 1425 555 501 343 54 6
MslI CAynn'nnrTG
Cuts at: 518 1166 3857 4216 4375 518
Size: 648 2691 359 159 2042
Fragments arranged by size:
2691 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2082 2474 3054 3087 3132 3315 3992 5140
Size: 476 392 580 33 45 183 677 1148
Cuts at: 5140 5385 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 580 476 392 245 183
45 33
NaeI GCC'GGC
Cuts at: 2028 2107 2685 2028
Size: 79 578 5242
Fragments arranged by size:
5242 578 79
NarI GG'CG_CC
Cuts at: 2030 2262 2677 2030
Size: 232 415 5252
Fragments arranged by size:
5252 415 232
NcoI C'CATG_G
Cuts at: 513 1065 2004 2412 2889 513
Size: 552 939 408 477 3523
Fragments arranged by size:
3523 939 552 477 408
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NgoMIV G'CCGG_C
Cuts at: 2026 2105 2683 2026
Size: 79 578 5242
Fragments arranged by size:
5242 578 79
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 2793 3257 4802 1132
Size: 97 1564 464 1545 2229
Fragments arranged by size:
2229 1564 1545 464 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5899
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspOMI G'GGCC_C
Cuts at: 2426 2797 2426
Size: 371 5528
PstI C_TGCA'G
Cuts at: 830 2865 3196 3251 830
Size: 2035 331 55 3478
Fragments arranged by size:
3478 2035 331 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
SacII CC_GC'GG
Cuts at: 2083 2475 2083
Size: 392 5507
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SapI GCTCTTCn'nnn_
Cuts at: 2594 2594
Size: 5899
SbfI CC_TGCA'GG
Cuts at: 2865 3251 2865
Size: 386 5513
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2861 3192 3247 4382 5063 5254 826
Size: 219 1816 331 55 1135 681 191 1471
Fragments arranged by size:
1816 1471 1135 681 331 219 191 55
SfoI GGC'GCC
Cuts at: 2031 2263 2678 2031
Size: 232 415 5252
Fragments arranged by size:
5252 415 232
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 2032 1700
Size: 332 5567
SmaI CCC'GGG
Cuts at: 1872 2695 2720 3066 3226 1872
Size: 823 25 346 160 4545
Fragments arranged by size:
4545 823 346 160 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4003 4904 5166 820
Size: 197 926 2060 901 262 1553
Fragments arranged by size:
2060 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 2579 1517
Size: 1062 4837
StyI C'CwwG_G
Cuts at: 513 1065 2004 2412 2574 2889 3338 513
Size: 552 939 408 162 315 449 3074
Fragments arranged by size:
3074 939 552 449 408 315 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3172 3926 3943 4096 4281 4726 325
Size: 1255 1592 754 17 153 185 445 1498
Fragments arranged by size:
1592 1498 1255 754 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2854 4145 5857
Size: 274 80 33 51 1443 877 1291 1712
Cuts at: 5857 96
Size: 138
Fragments arranged by size:
1712 1443 1291 877 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5859 5891 5859
Size: 32 5867
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2517 2517
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 2693 2718 3064 3224 1870
Size: 823 25 346 160 4545
Fragments arranged by size:
4545 823 346 160 25
XmnI GAAnn'nnTTC
Cuts at: 4028 4028
Size: 5899
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI