Restriction map for
pFN21AB5167
(Circular) (Six-base) MAPSORT of: pFN21AB5167.seq Check: 8456 from: 1 to: 6265
pFN21AB5167 6265 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3193 3193
Size: 6265
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2301 3525 3608 1937
Size: 364 1224 83 4594
Fragments arranged by size:
4594 1224 364 83
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2837 3065 4328 5712 5980 1632
Size: 1205 228 1263 1384 268 1917
Fragments arranged by size:
1917 1384 1263 1205 268 228
AfeI AGC'GCT
Cuts at: 2087 2118 2087
Size: 31 6234
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 2625 2805 5164 6141 1203
Size: 1422 180 2359 977 1327
Fragments arranged by size:
2359 1422 1327 977 180
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 2932 4008 5580 2932
Size: 1076 1572 3617
Fragments arranged by size:
3617 1572 1076
ApaI G_GGCC'C
Cuts at: 2688 2688
Size: 6265
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 3574 3800 3952 6061 1493
Size: 2081 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 3590 1870
Size: 73 1647 4545
Fragments arranged by size:
4545 1647 73
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3134 3182 3509 3586 5041
Size: 325 217 627 1347 48 327 77 1455
Cuts at: 5041 618
Size: 1842
Fragments arranged by size:
1842 1455 1347 627 327 325 217 77
48
BanII G_rGCy'C
Cuts at: 1391 2295 2688 3099 3584 1391
Size: 904 393 411 485 4072
Fragments arranged by size:
4072 904 485 411 393
BbeI G_GCGC'C
Cuts at: 3513 3513
Size: 6265
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2057 2112 2844 3258 3540 928
Size: 971 158 55 732 414 282 3653
Fragments arranged by size:
3653 971 732 414 282 158 55
BbvCI CC'TCA_GC
Cuts at: 3383 3383
Size: 6265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2612 2646 4456 4490 1903
Size: 34 675 34 1810 34 3678
Fragments arranged by size:
3678 1810 675 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2979 3314 4876
Size: 107 122 71 631 605 1307 335 1562
Cuts at: 4876 136
Size: 1525
Fragments arranged by size:
1562 1525 1307 631 605 335 122 107
71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 2180 2688 2792 4326 5482 1790
Size: 390 508 104 1534 1156 2573
Fragments arranged by size:
2573 1534 1156 508 390 104
BmgBI CAC'GTC
Cuts at: 1318 2626 2779 1318
Size: 1308 153 4804
Fragments arranged by size:
4804 1308 153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2337 3996 4954
Size: 416 53 83 399 24 920 1659 958
Cuts at: 4954 442
Size: 1753
Fragments arranged by size:
1753 1659 958 920 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 3496 2446
Size: 1050 5215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3488 4925 1239
Size: 2249 1437 2579
Fragments arranged by size:
2579 2249 1437
Bpu10I CC'TnA_GC
Cuts at: 3383 3383
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2373 3281 4390 5255 5553 2373
Size: 908 1109 865 298 3085
Fragments arranged by size:
3085 1109 908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3012 3510 4072 4454
Size: 53 83 186 936 1479 498 562 382
Cuts at: 4454 5089 275
Size: 635 1451
Fragments arranged by size:
1479 1451 936 635 562 498 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2960 2990 1178
Size: 30 165 30 1557 30 4453
Fragments arranged by size:
4453 1557 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2978 2981 3107 3278 3485 3512 3560
Size: 928 864 3 126 171 207 27 48
Cuts at: 3560 1186
Size: 3891
Fragments arranged by size:
3891 928 864 207 171 126 48 27
3
BseYI C'CCAG_C
Cuts at: 5468 5964 5468
Size: 496 5769
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3170 3377 3392 1163
Size: 573 1434 207 15 4036
Fragments arranged by size:
4036 1434 573 207 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 3370 3715 4476 5504 1540
Size: 275 186 57 1312 345 761 1028 2301
Fragments arranged by size:
2301 1312 1028 761 345 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 2295 2368 3210 3336 4326 4411 5482 2295
Size: 73 842 126 990 85 1071 3078
Fragments arranged by size:
3078 1071 990 842 126 85 73
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
BsmBI CGTCTCn'nnnn_
Cuts at: 3467 3467
Size: 6265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2295 2368 2688 2792 3099 3210
Size: 399 390 115 73 320 104 307 111
Cuts at: 3210 3336 3584 4326 4411 5482 1391
Size: 126 248 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 320 307 248
126 115 111 104 85 73
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3193 3399 3414 3620 844
Size: 2349 206 15 206 3489
Fragments arranged by size:
3489 2349 206 206 15
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 4152 1394
Size: 429 1729 600 3507
Fragments arranged by size:
3507 1729 600 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3115 3269 3362 4909 5096 1700
Size: 110 1305 154 93 1547 187 2869
Fragments arranged by size:
2869 1547 1305 187 154 110 93
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3331 794
Size: 2537 3728
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 3190 3376 1184
Size: 2006 186 4073
Fragments arranged by size:
4073 2006 186
BstYI r'GATC_y
Cuts at: 1972 2464 3595 3962 4347 4364 5816 5902 5914
Size: 492 1131 367 385 17 1452 86 12
Cuts at: 5914 6260 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1131 492 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2038 2888 2904 2910 3186 513
Size: 552 939 34 850 16 6 276 3592
Fragments arranged by size:
3592 939 850 552 276 34 16 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 3394 3781 4575 4595 3394
Size: 387 794 20 5064
Fragments arranged by size:
5064 794 387 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3374 5272 809
Size: 507 2058 1898 1802
Fragments arranged by size:
2058 1898 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2266 2484
Size: 54 1425 50 275 18 225 211 218
Cuts at: 2484 2907 3171 3712 4601 6230 8
Size: 423 264 541 889 1629 43
Fragments arranged by size:
1629 1425 889 541 423 275 264 225
218 211 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2055 3712 1537
Size: 275 243 1657 4090
Fragments arranged by size:
4090 1657 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 2555 2827 2955 3480 4195 1353
Size: 1202 272 128 525 715 3423
Fragments arranged by size:
3423 1202 715 525 272 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2412 4852 5226 5372 387
Size: 1064 138 823 2440 374 146 1280
Fragments arranged by size:
2440 1280 1064 823 374 146 138
EcoICRI GAG'CTC
Cuts at: 2293 2293
Size: 6265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2837 3065 3488 4328 4925 5712 5980
Size: 393 1205 228 423 840 597 787 268
Cuts at: 5980 1239
Size: 1524
Fragments arranged by size:
1524 1205 840 787 597 423 393 268
228
EcoNI CCTnn'n_nnAGG
Cuts at: 2191 3263 2191
Size: 1072 5193
EcoO109I rG'GnC_Cy
Cuts at: 2473 2684 3050 3143 2473
Size: 211 366 93 5595
Fragments arranged by size:
5595 366 211 93
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3275 3275
Size: 6265
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2089 2120 2362 3513 5412 1678
Size: 411 31 242 1151 1899 2531
Fragments arranged by size:
2531 1899 1151 411 242 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2527 2556 2559 2588 2960
Size: 32 135 32 955 29 3 29 372
Cuts at: 2960 2992 4906 4938 4980 5012 1373
Size: 32 1914 32 42 32 2626
Fragments arranged by size:
2626 1914 955 372 135 42 32 32
32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 3526 3609 3855 669
Size: 988 281 397 1191 83 246 3079
Fragments arranged by size:
3079 1191 988 397 281 246 83
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KasI G'GCGC_C
Cuts at: 3509 3509
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 2567 3864 2567
Size: 1297 4968
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2513 3327 5379 5563 1305
Size: 1208 814 2052 184 2007
Fragments arranged by size:
2052 2007 1208 814 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2486 2909 10
Size: 54 1425 343 654 423 3366
Fragments arranged by size:
3366 1425 654 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4223 4582 4741 518
Size: 648 3057 359 159 2042
Fragments arranged by size:
3057 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2404 3043 3154 3540 3681 4358 5506
Size: 434 364 639 111 386 141 677 1148
Cuts at: 5506 5751 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 639 434 386 364 245
141 111
NaeI GCC'GGC
Cuts at: 3117 3271 3364 3117
Size: 154 93 6018
Fragments arranged by size:
6018 154 93
NarI GG'CG_CC
Cuts at: 3510 3510
Size: 6265
NcoI C'CATG_G
Cuts at: 513 1065 2004 2910 513
Size: 552 939 906 3868
Fragments arranged by size:
3868 939 906 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NgoMIV G'CCGG_C
Cuts at: 3115 3269 3362 3115
Size: 154 93 6018
Fragments arranged by size:
6018 154 93
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2809 3355 3623 5168 1132
Size: 97 1580 546 268 1545 2229
Fragments arranged by size:
2229 1580 1545 546 268 97
PasI CC'CwG_GG
Cuts at: 1348 2725 3139 1348
Size: 1377 414 4474
Fragments arranged by size:
4474 1377 414
PciI A'CATG_T
Cuts at: 2805 5164 2805
Size: 2359 3906
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2496 2894 1108
Size: 1269 119 398 4479
Fragments arranged by size:
4479 1269 398 119
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2473 3050 2473
Size: 577 5688
PshAI GACnn'nnGTC
Cuts at: 1613 3055 1613
Size: 1442 4823
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspOMI G'GGCC_C
Cuts at: 2684 2684
Size: 6265
PstI C_TGCA'G
Cuts at: 830 3617 830
Size: 2787 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2404 3043 3154 2404
Size: 639 111 5515
Fragments arranged by size:
5515 639 111
SacI G_AGCT'C
Cuts at: 2295 2295
Size: 6265
SacII CC_GC'GG
Cuts at: 2041 2041
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3524 3607 1936
Size: 1588 83 4594
Fragments arranged by size:
4594 1588 83
SanDI GG'GwC_CC
Cuts at: 3050 3050
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 3613 4748 5429 5620 826
Size: 219 2568 1135 681 191 1471
Fragments arranged by size:
2568 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3511 3511
Size: 6265
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2388 3260 4369 5270 5532 820
Size: 197 926 445 872 1109 901 262 1553
Fragments arranged by size:
1553 1109 926 901 872 445 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 2258 4189 5
Size: 47 2206 1931 2081
Fragments arranged by size:
2206 2081 1931 47
StuI AGG'CCT
Cuts at: 1517 3431 1517
Size: 1914 4351
StyI C'CwwG_G
Cuts at: 513 1065 2004 2192 2910 3432 3704 513
Size: 552 939 188 718 522 272 3074
Fragments arranged by size:
3074 939 718 552 522 272 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2707 3152 4292 4309 4462 4647 5092
Size: 1255 1127 445 1140 17 153 185 445
Cuts at: 5092 325
Size: 1498
Fragments arranged by size:
1498 1255 1140 1127 445 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2597 3000 4511
Size: 274 80 33 51 1443 620 403 1511
Cuts at: 4511 6223 96
Size: 1712 138
Fragments arranged by size:
1712 1511 1443 620 403 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 6225 6257 2159
Size: 32 4034 32 2167
Fragments arranged by size:
4034 2167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3310 3310
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SapI SexAI SfiI
SrfI SwaI