Restriction map for
pFN21AB5164
(Circular) (Six-base) MAPSORT of: pFN21AB5164.seq Check: 9575 from: 1 to: 5986
pFN21AB5164 5986 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 4049 5433 5701 1632
Size: 968 1449 1384 268 1917
Fragments arranged by size:
1917 1449 1384 968 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 4885 5862 1203
Size: 3682 977 1327
Fragments arranged by size:
3682 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 2515 2747 2860 3729 5301 2515
Size: 232 113 869 1572 3200
Fragments arranged by size:
3200 1572 869 232 113
ApaI G_GGCC'C
Cuts at: 2565 2854 2976 3275 2565
Size: 289 122 299 5276
Fragments arranged by size:
5276 299 289 122
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2239 3295 3521 3673 5782 1493
Size: 746 1056 226 152 2109 1697
Fragments arranged by size:
2109 1697 1056 746 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2201 3141 3311 1870
Size: 73 258 940 170 4545
Fragments arranged by size:
4545 940 258 170 73
BamHI G'GATC_C
Cuts at: 2025 3316 3683 2025
Size: 1291 367 4328
Fragments arranged by size:
4328 1291 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2593 3152 3307 4762 618
Size: 325 217 627 806 559 155 1455 1842
Fragments arranged by size:
1842 1455 806 627 559 325 217 155
BanII G_rGCy'C
Cuts at: 1391 2502 2525 2565 2854 2976 3275 3305 1391
Size: 1111 23 40 289 122 299 30 4072
Fragments arranged by size:
4072 1111 299 289 122 40 30 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2421 2641 928
Size: 971 522 220 4273
Fragments arranged by size:
4273 971 522 220
BbvCI CC'TCA_GC
Cuts at: 2508 2508
Size: 5986
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2676 2725 4597
Size: 107 122 71 631 605 1004 49 1872
Cuts at: 4597 136
Size: 1525
Fragments arranged by size:
1872 1525 1004 631 605 122 107 71
49
BglII A'GATC_T
Cuts at: 2986 5981 2986
Size: 2995 2991
BlpI GC'TnA_GC
Cuts at: 2798 3403 2798
Size: 605 5381
Bme1580I G_kGCm'C
Cuts at: 1790 2565 2854 2868 2976 3111 3275 4047 5203
Size: 775 289 14 108 135 164 772 1156
Cuts at: 5203 1790
Size: 2573
Fragments arranged by size:
2573 1156 775 772 289 164 135 108
14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2444 2890 3717
Size: 416 53 83 399 24 1027 446 827
Cuts at: 3717 4675 442
Size: 958 1753
Fragments arranged by size:
1753 1027 958 827 446 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 3185 4646 1239
Size: 848 1098 1461 2579
Fragments arranged by size:
2579 1461 1098 848
Bpu10I CC'TnA_GC
Cuts at: 2496 2508 2496
Size: 12 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4111 4976 5274 4111
Size: 865 298 4823
Fragments arranged by size:
4823 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4649 882
Size: 617 3150 2219
Fragments arranged by size:
3150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2785 3114 3777 493
Size: 711 1581 329 663 2702
Fragments arranged by size:
2702 1581 711 663 329
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2289 2374 4419 4817 5091 5238 5649 2289
Size: 85 2045 398 274 147 411 2626
Fragments arranged by size:
2626 2045 411 398 274 147 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3191 3221 1178
Size: 30 165 30 1788 30 3943
Fragments arranged by size:
3943 1788 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2909 3148 1186
Size: 1723 239 4024
Fragments arranged by size:
4024 1723 239
BseYI C'CCAG_C
Cuts at: 2358 2525 2853 2860 5189 5685 2358
Size: 167 328 7 2329 496 2659
Fragments arranged by size:
2659 2329 496 328 167 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3167 1163
Size: 573 1431 3982
Fragments arranged by size:
3982 1431 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4047 4132 5203 4047
Size: 85 1071 4830
Fragments arranged by size:
4830 1071 85
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2502 2525 2565 2854 2868 2976 3111
Size: 399 712 23 40 289 14 108 135
Cuts at: 3111 3275 3305 4047 4132 5203 1391
Size: 164 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 712 399 289 164 135
108 85 40 30 23 14
BspEI T'CCGG_A
Cuts at: 2374 5649 2374
Size: 3275 2711
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 3189 3246 3341 844
Size: 1385 960 57 95 3489
Fragments arranged by size:
3489 1385 960 95 57
BsrBI CCG'CTC
Cuts at: 1394 1823 2247 3873 1394
Size: 429 424 1626 3507
Fragments arranged by size:
3507 1626 429 424
BsrDI GCAATG_nn'
Cuts at: 65 1248 4481 4655 65
Size: 1183 3233 174 1396
Fragments arranged by size:
3233 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 3089 4046 5058 3089
Size: 957 1012 4017
Fragments arranged by size:
4017 1012 957
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 3275 3275
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2025 2464 2986 3316 3683 4068 4085 5537
Size: 53 439 522 330 367 385 17 1452
Cuts at: 5537 5623 5635 5981 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 522 439 385 367 346 330
86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 3119 3119
Size: 5986
BtgI C'CryG_G
Cuts at: 513 1065 2004 2021 3074 513
Size: 552 939 17 1053 3425
Fragments arranged by size:
3425 1053 939 552 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2735 2846 2866 2892 3502 4296 4316 2735
Size: 111 20 26 610 794 20 4405
Fragments arranged by size:
4405 794 610 111 26 20 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2726 3433 4322
Size: 54 1425 50 275 18 896 707 889
Cuts at: 4322 5951 8
Size: 1629 43
Fragments arranged by size:
1629 1425 896 889 707 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 2598 3916 1353
Size: 875 370 1318 3423
Fragments arranged by size:
3423 1318 875 370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2600 3185 4049 4646 5433 5701
Size: 393 455 513 585 864 597 787 268
Cuts at: 5701 1239
Size: 1524
Fragments arranged by size:
1524 864 787 597 585 513 455 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2132 2233 2132
Size: 101 5885
EcoO109I rG'GnC_Cy
Cuts at: 2299 2471 2561 2562 2677 2935 2972 2973 3123
Size: 172 90 1 115 258 37 1 150
Cuts at: 3123 3271 3272 2299
Size: 148 1 5013
Fragments arranged by size:
5013 258 172 150 148 115 90 37
1 1 1
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 5133 1678
Size: 3455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2715 2747 2808 2840 4627
Size: 32 135 32 1143 32 61 32 1787
Cuts at: 4627 4659 4701 4733 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1787 1143 135 61 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3216 3243 5100 5284 1305
Size: 1911 27 1857 184 2007
Fragments arranged by size:
2007 1911 1857 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2728 10
Size: 54 1425 343 896 3268
Fragments arranged by size:
3268 1425 896 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3944 4303 4462 518
Size: 648 2778 359 159 2042
Fragments arranged by size:
2778 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2857 2913 3402 4079 5227 5472 1606
Size: 444 807 56 489 677 1148 245 2120
Fragments arranged by size:
2120 1148 807 677 489 444 245 56
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
NdeI CA'TA_TG
Cuts at: 387 2194 387
Size: 1807 4179
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2755 2795 3344 4889 1132
Size: 97 1526 40 549 1545 2229
Fragments arranged by size:
2229 1545 1526 549 97 40
PasI CC'CwG_GG
Cuts at: 1348 3275 1348
Size: 1927 4059
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2747 2860 1108
Size: 1639 113 4234
Fragments arranged by size:
4234 1639 113
PfoI T'CCnGG_A
Cuts at: 2467 2467
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PmlI CAC'GTG
Cuts at: 3114 3114
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 2629 4055 4087 5568 5600 2597
Size: 32 1426 32 1481 32 2983
Fragments arranged by size:
2983 1481 1426 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2471 2935 2299
Size: 172 464 5350
Fragments arranged by size:
5350 464 172
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PspOMI G'GGCC_C
Cuts at: 2561 2850 2972 3271 2561
Size: 289 122 299 5276
Fragments arranged by size:
5276 299 289 122
PstI C_TGCA'G
Cuts at: 830 3243 3338 830
Size: 2413 95 3478
Fragments arranged by size:
3478 2413 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2050 2050
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SanDI GG'GwC_CC
Cuts at: 2471 2471
Size: 5986
SbfI CC_TGCA'GG
Cuts at: 3243 3338 3243
Size: 95 5891
ScaI AGT'ACT
Cuts at: 2398 4234 5946 2398
Size: 1836 1712 2438
Fragments arranged by size:
2438 1836 1712
SfcI C'TryA_G
Cuts at: 826 1045 2487 3239 3334 4469 5150 5341 826
Size: 219 1442 752 95 1135 681 191 1471
Fragments arranged by size:
1471 1442 1135 752 681 219 191 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 2203 3143 3313 1872
Size: 331 940 170 4545
Fragments arranged by size:
4545 940 331 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4090 4991 5253 820
Size: 197 926 2147 901 262 1553
Fragments arranged by size:
2147 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 2171 3233 1517
Size: 654 1062 4270
Fragments arranged by size:
4270 1062 654
StyI C'CwwG_G
Cuts at: 513 1065 2004 2114 2940 3126 3425 513
Size: 552 939 110 826 186 299 3074
Fragments arranged by size:
3074 939 826 552 299 186 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2071 2798 3216 4013 4030 4183 4368
Size: 1255 491 727 418 797 17 153 185
Cuts at: 4368 4813 325
Size: 445 1498
Fragments arranged by size:
1498 1255 797 727 491 445 418 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2396 3225 4232
Size: 274 80 33 51 1443 419 829 1007
Cuts at: 4232 5944 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1007 829 419 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3059 3091 3191 3223 5946 5978 3059
Size: 32 100 32 2723 32 3067
Fragments arranged by size:
3067 2723 100 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 2201 3141 3311 1870
Size: 331 940 170 4545
Fragments arranged by size:
4545 940 331 170
XmnI GAAnn'nnTTC
Cuts at: 2418 4115 2418
Size: 1697 4289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI