Restriction map for
pFN21AB5162
(Circular) (Six-base) MAPSORT of: pFN21AB5162.seq Check: 7396 from: 1 to: 5356
pFN21AB5162 5356 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3166 278
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2699 1937
Size: 762 4594
Acc65I G'GTAC_C
Cuts at: 2677 2677
Size: 5356
AclI AA'CG_TT
Cuts at: 1563 3483 3856 1563
Size: 1920 373 3063
Fragments arranged by size:
3063 1920 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3419 4803 5071 1632
Size: 1787 1384 268 1917
Fragments arranged by size:
1917 1787 1384 268
AfeI AGC'GCT
Cuts at: 2275 2275
Size: 5356
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5159
AflIII A'CryG_T
Cuts at: 1203 4255 5232 1203
Size: 3052 977 1327
Fragments arranged by size:
3052 1327 977
AgeI A'CCGG_T
Cuts at: 4187 4187
Size: 5356
AhdI GACnn_n'nnGTC
Cuts at: 4085 4085
Size: 5356
AleI CACnn'nnGTG
Cuts at: 1166 2179 2639 1166
Size: 1013 460 3883
Fragments arranged by size:
3883 1013 460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5356
AlwNI CAG_nnn'CTG
Cuts at: 3099 4671 3099
Size: 1572 3784
ApaLI G'TGCA_C
Cuts at: 3413 4569 3413
Size: 1156 4200
ApoI r'AATT_y
Cuts at: 1493 2665 2891 3043 5152 1493
Size: 1172 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3910 160
Size: 872 2878 1606
Fragments arranged by size:
2878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
AvaI C'yCGr_G
Cuts at: 1870 1943 2681 1870
Size: 73 738 4545
Fragments arranged by size:
4545 738 73
BamHI G'GATC_C
Cuts at: 2686 3053 2686
Size: 367 4989
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2464 2677 4132 618
Size: 325 217 627 677 213 1455 1842
Fragments arranged by size:
1842 1455 677 627 325 217 213
BanII G_rGCy'C
Cuts at: 1391 2675 1391
Size: 1284 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3547 3581 1903
Size: 34 1610 34 3678
Fragments arranged by size:
3678 1610 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3248 4458 3248
Size: 1210 4146
BclI T'GATC_A
Cuts at: 1548 2101 1548
Size: 553 4803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3967 136
Size: 107 122 71 631 605 2295 1525
Fragments arranged by size:
2295 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2411 5351 2411
Size: 2940 2416
BlpI GC'TnA_GC
Cuts at: 2054 2225 2773 2054
Size: 171 548 4637
Fragments arranged by size:
4637 548 171
Bme1580I G_kGCm'C
Cuts at: 1790 3417 4573 1790
Size: 1627 1156 2573
Fragments arranged by size:
2573 1627 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3087 4045 442
Size: 416 53 83 399 24 1670 958 1753
Fragments arranged by size:
1753 1670 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2410 4016 1239
Size: 1171 1606 2579
Fragments arranged by size:
2579 1606 1171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 3481 4346 4644 2063
Size: 1418 865 298 2775
Fragments arranged by size:
2775 1418 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4019 882
Size: 617 2520 2219
Fragments arranged by size:
2520 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3147 493
Size: 711 1943 2702
Fragments arranged by size:
2702 1943 711
BsaBI GATnn'nnATC
Cuts at: 2416 5350 2416
Size: 2934 2422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3163 3545 4180 275
Size: 53 83 186 936 1630 382 635 1451
Fragments arranged by size:
1630 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2646 3789 4187 4461 4608 5019 2646
Size: 1143 398 274 147 411 2983
Fragments arranged by size:
2983 1143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5131
Fragments arranged by size:
5131 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5356
BseYI C'CCAG_C
Cuts at: 2527 4559 5055 2527
Size: 2032 496 2828
Fragments arranged by size:
2828 2032 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 1163
Size: 573 603 4180
Fragments arranged by size:
4180 603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2806 3567 4595 1540
Size: 275 186 805 761 1028 2301
Fragments arranged by size:
2301 1028 805 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 3417 3502 4573 2337
Size: 1080 85 1071 3120
Fragments arranged by size:
3120 1080 1071 85
BsmI GAATG_Cn'
Cuts at: 2867 2960 2867
Size: 93 5263
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2675 3417 3502 4573 1391
Size: 399 547 338 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 399 338 85
BspEI T'CCGG_A
Cuts at: 5019 5019
Size: 5356
BspHI T'CATG_A
Cuts at: 3245 4975 3245
Size: 1730 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2711 844
Size: 1867 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3243 1394
Size: 429 1420 3507
Fragments arranged by size:
3507 1420 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3851 4025 65
Size: 1183 2603 174 1396
Fragments arranged by size:
2603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2609 4000 4187 1700
Size: 110 799 1391 187 2869
Fragments arranged by size:
2869 1391 799 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5356
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5356
BssSI C'ACGA_G
Cuts at: 3416 4428 3416
Size: 1012 4344
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5356
BstBI TT'CG_AA
Cuts at: 4160 4160
Size: 5356
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5356
BstXI CCAn_nnnn'nTGG
Cuts at: 2181 2519 2181
Size: 338 5018
BstYI r'GATC_y
Cuts at: 1972 2411 2686 3053 3438 3455 4907 4993 5005
Size: 439 275 367 385 17 1452 86 12
Cuts at: 5005 5351 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 439 385 367 346 275 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2180 2599 513
Size: 552 939 176 419 3270
Fragments arranged by size:
3270 939 552 419 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 1999 487
Size: 683 56 773 3844
Fragments arranged by size:
3844 773 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 3666 3686 2872
Size: 794 20 4542
Fragments arranged by size:
4542 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5321
DraI TTT'AAA
Cuts at: 2660 2660
Size: 5356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4363 809
Size: 507 3047 1802
Fragments arranged by size:
3047 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2803 3692 5321
Size: 54 1425 50 275 18 973 889 1629
Cuts at: 5321 8
Size: 43
Fragments arranged by size:
1629 1425 973 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2803 1537
Size: 275 991 4090
Fragments arranged by size:
4090 991 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3286 1353
Size: 1933 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2525 3943 4317 4463 387
Size: 1064 138 936 1418 374 146 1280
Fragments arranged by size:
1418 1280 1064 936 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2410 3419 4016 4803 5071 1239
Size: 393 778 1009 597 787 268 1524
Fragments arranged by size:
1524 1009 787 778 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2247 2491 2247
Size: 244 5112
EcoRI G'AATT_C
Cuts at: 2665 3043 2665
Size: 378 4978
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2240 2240
Size: 5356
FspI TGC'GCA
Cuts at: 3862 3862
Size: 5356
HaeII r_GCGC'y
Cuts at: 1678 2277 4503 1678
Size: 599 2226 2531
Fragments arranged by size:
2531 2226 599
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2535 2567 3997 4029 4071
Size: 32 135 32 963 32 1430 32 42
Cuts at: 4071 4103 1373
Size: 32 2626
Fragments arranged by size:
2626 1430 963 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2700 2946 669
Size: 988 281 762 246 3079
Fragments arranged by size:
3079 988 762 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5356
HpaI GTT'AAC
Cuts at: 2946 2946
Size: 5356
KpnI G_GTAC'C
Cuts at: 2681 2681
Size: 5356
MfeI C'AATT_G
Cuts at: 2955 2955
Size: 5356
MluI A'CGCG_T
Cuts at: 5232 5232
Size: 5356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4470 4654 1305
Size: 3165 184 2007
Fragments arranged by size:
3165 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3534
Fragments arranged by size:
3534 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2179 2639 3314 3673 3832 518
Size: 648 999 14 460 675 359 159 2042
Fragments arranged by size:
2042 999 675 648 460 359 159 14
MspA1I CmG'CkG
Cuts at: 1606 2236 2772 3449 4597 4842 1606
Size: 630 536 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 630 536 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3865
Fragments arranged by size:
3865 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5356
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5356
NotI GC'GGCC_GC
Cuts at: 2803 2803
Size: 5356
NspI r_CATG'y
Cuts at: 1132 1229 2617 2714 4259 1132
Size: 97 1388 97 1545 2229
Fragments arranged by size:
2229 1545 1388 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5356
PciI A'CATG_T
Cuts at: 4255 4255
Size: 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 2583 1108
Size: 1452 23 3881
Fragments arranged by size:
3881 1452 23
PmeI GTTT'AAAC
Cuts at: 2660 2660
Size: 5356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 4938 4970 3425
Size: 32 1481 32 3811
Fragments arranged by size:
3811 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2483 1613
Size: 870 4486
PsiI TTA'TAA
Cuts at: 2926 2926
Size: 5356
PstI C_TGCA'G
Cuts at: 830 2044 2708 830
Size: 1214 664 3478
Fragments arranged by size:
3478 1214 664
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5356
SalI G'TCGA_C
Cuts at: 1936 2698 1936
Size: 762 4594
SbfI CC_TGCA'GG
Cuts at: 2708 2708
Size: 5356
ScaI AGT'ACT
Cuts at: 2357 3604 5316 2357
Size: 1247 1712 2397
Fragments arranged by size:
2397 1712 1247
SfcI C'TryA_G
Cuts at: 826 1045 2040 2704 3839 4520 4711 826
Size: 219 995 664 1135 681 191 1471
Fragments arranged by size:
1471 1135 995 681 664 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5356
SmaI CCC'GGG
Cuts at: 1872 2683 1872
Size: 811 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 3460 4361 4623 820
Size: 197 926 135 1382 901 262 1553
Fragments arranged by size:
1553 1382 926 901 262 197 135
SnaBI TAC'GTA
Cuts at: 493 3147 493
Size: 2654 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5356
SphI G_CATG'C
Cuts at: 1132 2714 1132
Size: 1582 3774
SspI AAT'ATT
Cuts at: 5 52 3280 5
Size: 47 3228 2081
Fragments arranged by size:
3228 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5356
StyI C'CwwG_G
Cuts at: 513 1065 2004 2208 2795 513
Size: 552 939 204 587 3074
Fragments arranged by size:
3074 939 587 552 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2356 3383 3400 3553 3738 4183 325
Size: 1255 776 1027 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1027 776 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 3602 5314
Size: 274 80 33 51 1443 378 1247 1712
Cuts at: 5314 96
Size: 138
Fragments arranged by size:
1712 1443 1247 378 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2301 2333 5316 5348 2301
Size: 32 2983 32 2309
Fragments arranged by size:
2983 2309 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5356
XbaI T'CTAG_A
Cuts at: 2692 2692
Size: 5356
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5356
XmaI C'CCGG_G
Cuts at: 1870 2681 1870
Size: 811 4545
XmnI GAAnn'nnTTC
Cuts at: 2357 3485 2357
Size: 1128 4228
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3164 276
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI