Restriction map for
pFN21AB5159
(Circular) (Six-base) MAPSORT of: pFN21AB5159.seq Check: 7500 from: 1 to: 5293
pFN21AB5159 5293 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2149 2149
Size: 5293
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2241 3103 278
Size: 53 83 186 936 705 862 2468
Fragments arranged by size:
2468 936 862 705 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2636 1937
Size: 699 4594
Acc65I G'GTAC_C
Cuts at: 2614 2614
Size: 5293
AclI AA'CG_TT
Cuts at: 1563 3420 3793 1563
Size: 1857 373 3063
Fragments arranged by size:
3063 1857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3356 4740 5008 1632
Size: 1724 1384 268 1917
Fragments arranged by size:
1917 1724 1384 268
AfeI AGC'GCT
Cuts at: 2549 2549
Size: 5293
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5096
AflIII A'CryG_T
Cuts at: 1203 4192 5169 1203
Size: 2989 977 1327
Fragments arranged by size:
2989 1327 977
AgeI A'CCGG_T
Cuts at: 4124 4124
Size: 5293
AhdI GACnn_n'nnGTC
Cuts at: 4022 4022
Size: 5293
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5293
AlwNI CAG_nnn'CTG
Cuts at: 3036 4608 3036
Size: 1572 3721
ApaLI G'TGCA_C
Cuts at: 3350 4506 3350
Size: 1156 4137
ApoI r'AATT_y
Cuts at: 1493 2602 2828 2980 5089 1493
Size: 1109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3847 160
Size: 872 2815 1606
Fragments arranged by size:
2815 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2291 2306 2474 2618 1870
Size: 73 153 195 15 168 144 4545
Fragments arranged by size:
4545 195 168 153 144 73 15
BamHI G'GATC_C
Cuts at: 2623 2990 2623
Size: 367 4926
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2614 4069 618
Size: 325 217 627 827 1455 1842
Fragments arranged by size:
1842 1455 827 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2612 1391
Size: 1221 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 2506 928
Size: 971 211 396 3715
Fragments arranged by size:
3715 971 396 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2465 2499 3484 3518 1903
Size: 34 528 34 985 34 3678
Fragments arranged by size:
3678 985 528 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3185 4395 3185
Size: 1210 4083
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3904 136
Size: 107 122 71 631 605 337 1895 1525
Fragments arranged by size:
1895 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5288 5288
Size: 5293
BlpI GC'TnA_GC
Cuts at: 2710 2710
Size: 5293
Bme1580I G_kGCm'C
Cuts at: 1790 2112 2337 3354 4510 1790
Size: 322 225 1017 1156 2573
Fragments arranged by size:
2573 1156 1017 322 225
BmgBI CAC'GTC
Cuts at: 1318 2061 1318
Size: 743 4550
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3024 3982 442
Size: 416 53 83 399 24 1607 958 1753
Fragments arranged by size:
1753 1607 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 3953 1239
Size: 1061 1653 2579
Fragments arranged by size:
2579 1653 1061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3418 4283 4581 3418
Size: 865 298 4130
Fragments arranged by size:
4130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3956 882
Size: 617 2457 2219
Fragments arranged by size:
2457 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2107 3084 493
Size: 711 903 977 2702
Fragments arranged by size:
2702 977 903 711
BsaBI GATnn'nnATC
Cuts at: 5287 5287
Size: 5293
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2238 3100 3482 4117
Size: 53 83 186 936 705 862 382 635
Cuts at: 4117 275
Size: 1451
Fragments arranged by size:
1451 936 862 705 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3726 4124 4398 4545 4956 3726
Size: 398 274 147 411 4063
Fragments arranged by size:
4063 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5068
Fragments arranged by size:
5068 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5293
BseYI C'CCAG_C
Cuts at: 4496 4992 4496
Size: 496 4797
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4720
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2206 2216 2437 2743 3504 4532
Size: 275 186 205 10 221 306 761 1028
Cuts at: 4532 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 306 275 221 205 186
10
BsiHKAI G_wGCw'C
Cuts at: 2483 3354 3439 4510 2483
Size: 871 85 1071 3266
Fragments arranged by size:
3266 1071 871 85
BsmI GAATG_Cn'
Cuts at: 2804 2897 2804
Size: 93 5200
BsmBI CGTCTCn'nnnn_
Cuts at: 2513 2513
Size: 5293
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2337 2483 2612 3354 3439 4510
Size: 399 322 225 146 129 742 85 1071
Cuts at: 4510 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 322 225 146 129
85
BspEI T'CCGG_A
Cuts at: 4956 4956
Size: 5293
BspHI T'CATG_A
Cuts at: 3182 4912 3182
Size: 1730 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2149 2373 2648 844
Size: 1305 224 275 3489
Fragments arranged by size:
3489 1305 275 224
BsrBI CCG'CTC
Cuts at: 1394 1823 2218 3180 1394
Size: 429 395 962 3507
Fragments arranged by size:
3507 962 429 395
BsrDI GCAATG_nn'
Cuts at: 65 1248 3788 3962 65
Size: 1183 2540 174 1396
Fragments arranged by size:
2540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3937 4124 1700
Size: 110 2127 187 2869
Fragments arranged by size:
2869 2127 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5293
BssHII G'CGCG_C
Cuts at: 1925 2530 1925
Size: 605 4688
BssSI C'ACGA_G
Cuts at: 3353 4365 3353
Size: 1012 4281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2061 2577 794
Size: 1267 516 3510
Fragments arranged by size:
3510 1267 516
BstBI TT'CG_AA
Cuts at: 4097 4097
Size: 5293
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5293
BstXI CCAn_nnnn'nTGG
Cuts at: 2344 2344
Size: 5293
BstYI r'GATC_y
Cuts at: 1972 2623 2990 3375 3392 4844 4930 4942 5288
Size: 651 367 385 17 1452 86 12 346
Cuts at: 5288 1972
Size: 1977
Fragments arranged by size:
1977 1452 651 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2442 513
Size: 552 939 438 3364
Fragments arranged by size:
3364 939 552 438
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4554
Fragments arranged by size:
4554 683 56
BtsI GCAGTG_nn'
Cuts at: 2809 3603 3623 2809
Size: 794 20 4479
Fragments arranged by size:
4479 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5258
DraI TTT'AAA
Cuts at: 2597 2597
Size: 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2237 2381 4300 809
Size: 507 921 144 1919 1802
Fragments arranged by size:
1919 1802 921 507 144
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2133 2213
Size: 54 1425 50 275 18 180 123 80
Cuts at: 2213 2349 2434 2445 2523 2740 3629 5258 8
Size: 136 85 11 78 217 889 1629 43
Fragments arranged by size:
1629 1425 889 275 217 180 136 123
85 80 78 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2213 2434 2740 1537
Size: 275 401 221 306 4090
Fragments arranged by size:
4090 401 306 275 221
EarI CTCTTCn'nnn_
Cuts at: 1353 3223 1353
Size: 1870 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3880 4254 4400 387
Size: 1064 138 2291 374 146 1280
Fragments arranged by size:
2291 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 3356 3953 4740 5008 1239
Size: 393 668 1056 597 787 268 1524
Fragments arranged by size:
1524 1056 787 668 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2302 2302
Size: 5293
EcoRI G'AATT_C
Cuts at: 2602 2980 2602
Size: 378 4915
FspI TGC'GCA
Cuts at: 2369 2423 2453 3799 2369
Size: 54 30 1346 3863
Fragments arranged by size:
3863 1346 54 30
FspAI rTGC'GCAy
Cuts at: 2423 2453 2423
Size: 30 5263
HaeII r_GCGC'y
Cuts at: 1678 2174 2551 4440 1678
Size: 496 377 1889 2531
Fragments arranged by size:
2531 1889 496 377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3934 3966 4008 4040 1373
Size: 32 135 32 2362 32 42 32 2626
Fragments arranged by size:
2626 2362 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2883 669
Size: 988 281 699 246 3079
Fragments arranged by size:
3079 988 699 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5293
HpaI GTT'AAC
Cuts at: 2883 2883
Size: 5293
KpnI G_GTAC'C
Cuts at: 2618 2618
Size: 5293
MfeI C'AATT_G
Cuts at: 2892 2892
Size: 5293
MluI A'CGCG_T
Cuts at: 5169 5169
Size: 5293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4407 4591 1305
Size: 3102 184 2007
Fragments arranged by size:
3102 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 10
Size: 54 1425 343 519 2952
Fragments arranged by size:
2952 1425 519 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2246 2345 2447 3251 3610 3769
Size: 648 978 102 99 102 804 359 159
Cuts at: 3769 518
Size: 2042
Fragments arranged by size:
2042 978 804 648 359 159 102 102
99
MspA1I CmG'CkG
Cuts at: 1606 2709 3386 4534 4779 1606
Size: 1103 677 1148 245 2120
Fragments arranged by size:
2120 1148 1103 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2442 513
Size: 552 939 438 3364
Fragments arranged by size:
3364 939 552 438
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5293
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5293
NotI GC'GGCC_GC
Cuts at: 2213 2740 2213
Size: 527 4766
NspI r_CATG'y
Cuts at: 1132 1229 2384 2651 4196 1132
Size: 97 1155 267 1545 2229
Fragments arranged by size:
2229 1545 1155 267 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5293
PciI A'CATG_T
Cuts at: 4192 4192
Size: 5293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5293
PmeI GTTT'AAAC
Cuts at: 2597 2597
Size: 5293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 4875 4907 3362
Size: 32 1481 32 3748
Fragments arranged by size:
3748 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 2302
Size: 5293
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5293
PsiI TTA'TAA
Cuts at: 2863 2863
Size: 5293
PspXI vC'TCGA_Gb
Cuts at: 2474 2474
Size: 5293
PstI C_TGCA'G
Cuts at: 830 2544 2645 830
Size: 1714 101 3478
Fragments arranged by size:
3478 1714 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5293
SalI G'TCGA_C
Cuts at: 1936 2635 1936
Size: 699 4594
SanDI GG'GwC_CC
Cuts at: 2302 2302
Size: 5293
SbfI CC_TGCA'GG
Cuts at: 2645 2645
Size: 5293
ScaI AGT'ACT
Cuts at: 3541 5253 3541
Size: 1712 3581
SfcI C'TryA_G
Cuts at: 826 1045 2540 2641 3776 4457 4648 826
Size: 219 1495 101 1135 681 191 1471
Fragments arranged by size:
1495 1471 1135 681 219 191 101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5293
SmaI CCC'GGG
Cuts at: 1872 2620 1872
Size: 748 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 3397 4298 4560 820
Size: 197 926 531 923 901 262 1553
Fragments arranged by size:
1553 926 923 901 531 262 197
SnaBI TAC'GTA
Cuts at: 493 3084 493
Size: 2591 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5293
SphI G_CATG'C
Cuts at: 1132 2651 1132
Size: 1519 3774
SspI AAT'ATT
Cuts at: 5 52 3217 5
Size: 47 3165 2081
Fragments arranged by size:
3165 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5293
StyI C'CwwG_G
Cuts at: 513 1065 2004 2442 2493 2732 513
Size: 552 939 438 51 239 3074
Fragments arranged by size:
3074 939 552 438 239 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3320 3337 3490 3675 4120 325
Size: 1255 1740 17 153 185 445 1498
Fragments arranged by size:
1740 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3539 5251 96
Size: 274 80 33 51 1443 1562 1712 138
Fragments arranged by size:
1712 1562 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5253 5285 5253
Size: 32 5261
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5293
XbaI T'CTAG_A
Cuts at: 2629 2629
Size: 5293
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 2344
Size: 5293
XhoI C'TCGA_G
Cuts at: 1943 2474 1943
Size: 531 4762
XmaI C'CCGG_G
Cuts at: 1870 2618 1870
Size: 748 4545
XmnI GAAnn'nnTTC
Cuts at: 2318 3422 2318
Size: 1104 4189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2239 3101 276
Size: 53 83 186 936 705 862 2468
Fragments arranged by size:
2468 936 862 705 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI