Restriction map for
pFN21AB5154
(Circular) (Six-base) MAPSORT of: pFN21AB5154.seq Check: 1763 from: 1 to: 6589
pFN21AB5154 6589 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3459 3834 4399 278
Size: 53 83 186 936 1923 375 565 2468
Fragments arranged by size:
2468 1923 936 565 375 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2413 2837 3932 1937
Size: 476 424 1095 4594
Fragments arranged by size:
4594 1095 476 424
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2205 3504 3623 4652 6036 6304 1632
Size: 573 1299 119 1029 1384 268 1917
Fragments arranged by size:
1917 1384 1299 1029 573 268 119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 5488 6465 1203
Size: 4285 977 1327
Fragments arranged by size:
4285 1327 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 5318 5318
Size: 6589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6589
AlwNI CAG_nnn'CTG
Cuts at: 2027 3129 4332 5904 2027
Size: 1102 1203 1572 2712
Fragments arranged by size:
2712 1572 1203 1102
ApaLI G'TGCA_C
Cuts at: 4646 5802 4646
Size: 1156 5433
ApoI r'AATT_y
Cuts at: 1493 2762 2848 3368 3542 3898 4124 4276 6385
Size: 1269 86 520 174 356 226 152 2109
Cuts at: 6385 1493
Size: 1697
Fragments arranged by size:
2109 1697 1269 520 356 226 174 152
86
AseI AT'TA_AT
Cuts at: 160 1032 3474 5143 160
Size: 872 2442 1669 1606
Fragments arranged by size:
2442 1669 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 3068 3914 1870
Size: 73 1125 846 4545
Fragments arranged by size:
4545 1125 846 73
AvrII C'CTAG_G
Cuts at: 3334 3334
Size: 6589
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 3260 3803 3910 5365
Size: 325 217 627 255 1218 543 107 1455
Cuts at: 5365 618
Size: 1842
Fragments arranged by size:
1842 1455 1218 627 543 325 255 217
107
BanII G_rGCy'C
Cuts at: 1391 2169 2306 3172 3908 1391
Size: 778 137 866 736 4072
Fragments arranged by size:
4072 866 778 736 137
BbeI G_GCGC'C
Cuts at: 3807 3807
Size: 6589
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2573 3492 928
Size: 971 674 919 4025
Fragments arranged by size:
4025 971 919 674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2266 2284 3765 4481 5691 2266
Size: 18 1481 716 1210 3164
Fragments arranged by size:
3164 1481 1210 716 18
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5200 136
Size: 107 122 71 631 605 337 3191 1525
Fragments arranged by size:
3191 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3183 6584 3183
Size: 3401 3188
BlpI GC'TnA_GC
Cuts at: 2970 3042 4006 2970
Size: 72 964 5553
Fragments arranged by size:
5553 964 72
Bme1580I G_kGCm'C
Cuts at: 1790 4650 5806 1790
Size: 2860 1156 2573
Fragments arranged by size:
2860 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2163 2626 3169
Size: 416 53 83 399 24 746 463 543
Cuts at: 3169 3326 4320 5278 442
Size: 157 994 958 1753
Fragments arranged by size:
1753 994 958 746 543 463 416 399
157 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 3258 2161
Size: 1097 5492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2154 3132 5249 1239
Size: 915 978 2117 2579
Fragments arranged by size:
2579 2117 978 915
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4714 5579 5877 4714
Size: 865 298 5426
Fragments arranged by size:
5426 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2635 3108 5252 882
Size: 617 1136 473 2144 2219
Fragments arranged by size:
2219 2144 1136 617 473
BsaAI yAC'GTr
Cuts at: 493 1204 3844 4380 493
Size: 711 2640 536 2702
Fragments arranged by size:
2702 2640 711 536
BsaBI GATnn'nnATC
Cuts at: 2559 3024 6583 2559
Size: 465 3559 2565
Fragments arranged by size:
3559 2565 465
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3456 3804 3831 4396
Size: 53 83 186 936 1923 348 27 565
Cuts at: 4396 4778 5413 275
Size: 382 635 1451
Fragments arranged by size:
1923 1451 936 635 565 382 348 186
83 53 27
BsaWI w'CCGG_w
Cuts at: 2481 5022 5420 5694 5841 6252 2481
Size: 2541 398 274 147 411 2818
Fragments arranged by size:
2818 2541 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3856 3886 1178
Size: 30 165 30 2453 30 3881
Fragments arranged by size:
3881 2453 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2118 2907 1186
Size: 932 789 4868
Fragments arranged by size:
4868 932 789
BseYI C'CCAG_C
Cuts at: 2267 3090 3327 3694 3798 5792 6288 2267
Size: 823 237 367 104 1994 496 2568
Fragments arranged by size:
2568 1994 823 496 367 237 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3703 1163
Size: 573 1967 4049
Fragments arranged by size:
4049 1967 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2067 4039 4800 5828 1540
Size: 275 186 66 1972 761 1028 2301
Fragments arranged by size:
2301 1972 1028 761 275 186 66
BsiHKAI G_wGCw'C
Cuts at: 3172 3819 4650 4735 5806 3172
Size: 647 831 85 1071 3955
Fragments arranged by size:
3955 1071 831 647 85
BsmI GAATG_Cn'
Cuts at: 4100 4193 4100
Size: 93 6496
BsmBI CGTCTCn'nnnn_
Cuts at: 3447 3447
Size: 6589
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2306 3172 3819 3908 4650 4735
Size: 399 379 137 866 647 89 742 85
Cuts at: 4735 5806 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 866 742 647 399 379 137
89 85
BspEI T'CCGG_A
Cuts at: 2481 6252 2481
Size: 3771 2818
BspHI T'CATG_A
Cuts at: 2421 4478 6208 2421
Size: 2057 1730 2802
Fragments arranged by size:
2802 2057 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3944 844
Size: 3100 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2063 2126 4476 1394
Size: 429 240 63 2350 3507
Fragments arranged by size:
3507 2350 429 240 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 2357 2440 3506 5084 5258 65
Size: 1183 1109 83 1066 1578 174 1396
Fragments arranged by size:
1578 1396 1183 1109 1066 174 83
BsrFI r'CCGG_y
Cuts at: 1700 1810 2104 3195 5233 5420 1700
Size: 110 294 1091 2038 187 2869
Fragments arranged by size:
2869 2038 1091 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 2363 3708 96
Size: 2267 1345 2977
Fragments arranged by size:
2977 2267 1345
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2451 794
Size: 1657 4932
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstXI CCAn_nnnn'nTGG
Cuts at: 2462 3419 2462
Size: 957 5632
BstYI r'GATC_y
Cuts at: 1972 2142 2725 2740 3183 3766 3919 4286 4671
Size: 170 583 15 443 583 153 367 385
Cuts at: 4671 4688 6140 6226 6238 6584 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 583 583 443 385 367 346
170 153 86 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2307 3807 3878 513
Size: 552 939 303 1500 71 3224
Fragments arranged by size:
3224 1500 939 552 303 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5850
Fragments arranged by size:
5850 683 56
BtsI GCAGTG_nn'
Cuts at: 2833 4105 4899 4919 2833
Size: 1272 794 20 4503
Fragments arranged by size:
4503 1272 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 2853 3893 2853
Size: 1040 5549
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2539 3502 5596 809
Size: 507 1223 963 2094 1802
Fragments arranged by size:
2094 1802 1223 963 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2323 2523
Size: 54 1425 50 275 18 199 294 200
Cuts at: 2523 2660 4036 4925 6554 8
Size: 137 1376 889 1629 43
Fragments arranged by size:
1629 1425 1376 889 294 275 200 199
137 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4036 1537
Size: 275 2224 4090
Fragments arranged by size:
4090 2224 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2310 3439 3590 4519 1353
Size: 729 228 1129 151 929 3423
Fragments arranged by size:
3423 1129 929 729 228 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3400 5176 5550 5696 387
Size: 1064 138 1811 1776 374 146 1280
Fragments arranged by size:
1811 1776 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3170 3170
Size: 6589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2205 3132 3504 3623 4652 5249
Size: 393 522 51 927 372 119 1029 597
Cuts at: 5249 6036 6304 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1029 927 787 597 522 393 372
268 119 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2055 2055
Size: 6589
EcoO109I rG'GnC_Cy
Cuts at: 2024 2782 3237 2024
Size: 758 455 5376
Fragments arranged by size:
5376 758 455
EcoRI G'AATT_C
Cuts at: 3898 4276 3898
Size: 378 6211
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 2977 3807 5736 1678
Size: 1299 830 1929 2531
Fragments arranged by size:
2531 1929 1299 830
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2127 2159 2219 2251 2278
Size: 32 135 32 555 32 60 32 27
Cuts at: 2278 2310 2390 2422 3856 3888 5230 5262 5304
Size: 32 80 32 1434 32 1342 32 42
Cuts at: 5304 5336 1373
Size: 32 2626
Fragments arranged by size:
2626 1434 1342 555 135 80 60 42
32 32 32 32 32 32 32 32
32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 3933 4179 669
Size: 988 281 1995 246 3079
Fragments arranged by size:
3079 1995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6589
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KasI G'GCGC_C
Cuts at: 3803 3803
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 4188 4188
Size: 6589
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2180 3190 5703 5887 1305
Size: 875 1010 2513 184 2007
Fragments arranged by size:
2513 2007 1010 875 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2031 2525 2662 10
Size: 54 1425 343 199 494 137 3937
Fragments arranged by size:
3937 1425 494 343 199 137 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2460 4547 4906 5065 518
Size: 648 1294 2087 359 159 2042
Fragments arranged by size:
2087 2042 1294 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2114 2880 3308 4005 4682 5830 6075 1606
Size: 508 766 428 697 677 1148 245 2120
Fragments arranged by size:
2120 1148 766 697 677 508 428 245
NarI GG'CG_CC
Cuts at: 3804 3804
Size: 6589
NcoI C'CATG_G
Cuts at: 513 1065 2004 3878 513
Size: 552 939 1874 3224
Fragments arranged by size:
3224 1874 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 3947 5492 1132
Size: 97 2718 1545 2229
Fragments arranged by size:
2718 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6589
PciI A'CATG_T
Cuts at: 5488 5488
Size: 6589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6589
PfoI T'CCnGG_A
Cuts at: 2131 3109 2131
Size: 978 5611
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PmlI CAC'GTG
Cuts at: 3844 3844
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2024 2782 2024
Size: 758 5831
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 3636 4159 3636
Size: 523 6066
PstI C_TGCA'G
Cuts at: 830 3941 830
Size: 3111 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
SacI G_AGCT'C
Cuts at: 3172 3172
Size: 6589
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SapI GCTCTTCn'nnn_
Cuts at: 3590 3590
Size: 6589
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SfcI C'TryA_G
Cuts at: 826 1045 2431 3121 3937 5072 5753 5944 826
Size: 219 1386 690 816 1135 681 191 1471
Fragments arranged by size:
1471 1386 1135 816 690 681 219 191
SfoI GGC'GCC
Cuts at: 3805 3805
Size: 6589
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 3916 1872
Size: 2044 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4693 5594 5856 820
Size: 197 926 2750 901 262 1553
Fragments arranged by size:
2750 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 2840 152
Size: 2688 3901
SphI G_CATG'C
Cuts at: 1132 3947 1132
Size: 2815 3774
SspI AAT'ATT
Cuts at: 5 52 3630 4513 5
Size: 47 3578 883 2081
Fragments arranged by size:
3578 2081 883 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 2004 3334 3878 4028 513
Size: 552 939 1330 544 150 3074
Fragments arranged by size:
3074 1330 939 552 544 150
SwaI ATTT'AAAT
Cuts at: 2853 2853
Size: 6589
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2324 2736 4616 4633 4786 4971 5416
Size: 1255 744 412 1880 17 153 185 445
Cuts at: 5416 325
Size: 1498
Fragments arranged by size:
1880 1498 1255 744 445 412 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2363 3708 4835
Size: 274 80 33 51 1443 386 1345 1127
Cuts at: 4835 6547 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1345 1127 386 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6549 6581 6549
Size: 32 6557
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6589
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3416 3416
Size: 6589
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 3914 1870
Size: 2044 4545
XmnI GAAnn'nnTTC
Cuts at: 4718 4718
Size: 6589
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3457 3832 4397 276
Size: 53 83 186 936 1923 375 565 2468
Fragments arranged by size:
2468 1923 936 565 375 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI