Restriction map for
pFN21AB5152
(Circular) (Six-base) MAPSORT of: pFN21AB5152.seq Check: 5370 from: 1 to: 6667
pFN21AB5152 6667 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3458 3458
Size: 6667
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4477 278
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4010 1937
Size: 2073 4594
Acc65I G'GTAC_C
Cuts at: 3988 3988
Size: 6667
AclI AA'CG_TT
Cuts at: 1563 4794 5167 1563
Size: 3231 373 3063
Fragments arranged by size:
3231 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 2212 3587 4730 6114 6382 1632
Size: 421 159 1375 1143 1384 268 1917
Fragments arranged by size:
1917 1384 1375 1143 421 268 159
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6470
AflIII A'CryG_T
Cuts at: 1203 3525 5566 6543 1203
Size: 2322 2041 977 1327
Fragments arranged by size:
2322 2041 1327 977
AgeI A'CCGG_T
Cuts at: 5498 5498
Size: 6667
AhdI GACnn_n'nnGTC
Cuts at: 5396 5396
Size: 6667
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6667
AlwNI CAG_nnn'CTG
Cuts at: 3091 3536 4410 5982 3091
Size: 445 874 1572 3776
Fragments arranged by size:
3776 1572 874 445
ApaI G_GGCC'C
Cuts at: 2415 2718 3583 2415
Size: 303 865 5499
Fragments arranged by size:
5499 865 303
ApaLI G'TGCA_C
Cuts at: 4724 5880 4724
Size: 1156 5511
ApoI r'AATT_y
Cuts at: 1493 3976 4202 4354 6463 1493
Size: 2483 226 152 2109 1697
Fragments arranged by size:
2483 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5221 160
Size: 872 4189 1606
Fragments arranged by size:
4189 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
AvaI C'yCGr_G
Cuts at: 1870 1943 2408 2695 2964 3114 3347 3576 3672
Size: 73 465 287 269 150 233 229 96
Cuts at: 3672 3992 1870
Size: 320 4545
Fragments arranged by size:
4545 465 320 287 269 233 229 150
96 73
BamHI G'GATC_C
Cuts at: 3997 4364 3997
Size: 367 6300
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2237 2321 3131 3413
Size: 325 217 627 239 211 84 810 282
Cuts at: 3413 3470 3988 5443 618
Size: 57 518 1455 1842
Fragments arranged by size:
1842 1455 810 627 518 325 282 239
217 211 84 57
BanII G_rGCy'C
Cuts at: 1391 2052 2415 2685 2718 2735 2865 2963 3080
Size: 661 363 270 33 17 130 98 117
Cuts at: 3080 3127 3583 3986 1391
Size: 47 456 403 4072
Fragments arranged by size:
4072 661 456 403 363 270 130 117
98 47 33 17
BbeI G_GCGC'C
Cuts at: 2030 2325 2030
Size: 295 6372
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2534 3192 928
Size: 971 635 658 4403
Fragments arranged by size:
4403 971 658 635
BbvCI CC'TCA_GC
Cuts at: 2674 2887 2674
Size: 213 6454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2997 3031 4858 4892 1903
Size: 34 1060 34 1827 34 3678
Fragments arranged by size:
3678 1827 1060 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4559 5769 4559
Size: 1210 5457
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6667
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2123 2706 2772
Size: 107 122 71 631 605 451 583 66
Cuts at: 2772 2958 3363 3411 3479 3578 5278 136
Size: 186 405 48 68 99 1700 1525
Fragments arranged by size:
1700 1525 631 605 583 451 405 186
122 107 99 71 68 66 48
BglII A'GATC_T
Cuts at: 6662 6662
Size: 6667
BlpI GC'TnA_GC
Cuts at: 4084 4084
Size: 6667
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2240 2415 2718 3236 3583 3963 4728
Size: 249 201 175 303 518 347 380 765
Cuts at: 4728 5884 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 765 518 380 347 303 249
201 175
BmgBI CAC'GTC
Cuts at: 1318 3528 1318
Size: 2210 4457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2440 4398 5356
Size: 416 53 83 399 24 1023 1958 958
Cuts at: 5356 442
Size: 1753
Fragments arranged by size:
1958 1753 1023 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2707 2902 5327 1239
Size: 1468 195 2425 2579
Fragments arranged by size:
2579 2425 1468 195
Bpu10I CC'TnA_GC
Cuts at: 2170 2569 2674 2887 3935 2170
Size: 399 105 213 1048 4902
Fragments arranged by size:
4902 1048 399 213 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 3022 4792 5657 5955 2174
Size: 848 1770 865 298 2886
Fragments arranged by size:
2886 1770 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3164 3891 5330 882
Size: 617 1665 727 1439 2219
Fragments arranged by size:
2219 1665 1439 727 617
BsaAI yAC'GTr
Cuts at: 493 1204 2650 2904 3211 4458 493
Size: 711 1446 254 307 1247 2702
Fragments arranged by size:
2702 1446 1247 711 307 254
BsaBI GATnn'nnATC
Cuts at: 6661 6661
Size: 6667
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2322 2913 4474
Size: 53 83 186 936 494 295 591 1561
Cuts at: 4474 4856 5491 275
Size: 382 635 1451
Fragments arranged by size:
1561 1451 936 635 591 494 382 295
186 83 53
BsaWI w'CCGG_w
Cuts at: 2523 5100 5498 5772 5919 6330 2523
Size: 2577 398 274 147 411 2860
Fragments arranged by size:
2860 2577 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 2835 2865 3512
Size: 30 165 30 1038 30 364 30 647
Cuts at: 3512 3542 1178
Size: 30 4303
Fragments arranged by size:
4303 1038 647 364 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2194 2875 3518 3533 3563 3566 1186
Size: 1008 681 643 15 30 3 4287
Fragments arranged by size:
4287 1008 681 643 30 15 3
BseYI C'CCAG_C
Cuts at: 2165 2216 2564 2621 2722 3143 3307 3401 3718
Size: 51 348 57 101 421 164 94 317
Cuts at: 3718 5870 6366 2165
Size: 2152 496 2466
Fragments arranged by size:
2466 2152 496 421 348 317 164 101
94 57 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2651 2792 3722 3764 1163
Size: 573 915 141 930 42 4066
Fragments arranged by size:
4066 930 915 573 141 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3483 4117 4878 5906 1540
Size: 275 186 1482 634 761 1028 2301
Fragments arranged by size:
2301 1482 1028 761 634 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 2685 3216 4728 4813 5884 2052
Size: 633 531 1512 85 1071 2835
Fragments arranged by size:
2835 1512 1071 633 531 85
BsiWI C'GTAC_G
Cuts at: 2904 2904
Size: 6667
BsmI GAATG_Cn'
Cuts at: 4178 4271 4178
Size: 93 6574
BsmBI CGTCTCn'nnnn_
Cuts at: 2337 2920 2337
Size: 583 6084
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2052 2240 2415 2685 2718 2735
Size: 399 249 13 188 175 270 33 17
Cuts at: 2735 2865 2963 3080 3127 3216 3236 3583 3963
Size: 130 98 117 47 89 20 347 380
Cuts at: 3963 3986 4728 4813 5884 1391
Size: 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 347 270 249
188 175 130 117 98 89 85 47
33 23 20 17 13
BspEI T'CCGG_A
Cuts at: 2523 6330 2523
Size: 3807 2860
BspHI T'CATG_A
Cuts at: 4556 6286 4556
Size: 1730 4937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3458 4022 844
Size: 2614 564 3489
Fragments arranged by size:
3489 2614 564
BsrBI CCG'CTC
Cuts at: 1394 1823 2310 2317 2486 3640 3679 3829 4554
Size: 429 487 7 169 1154 39 150 725
Cuts at: 4554 1394
Size: 3507
Fragments arranged by size:
3507 1154 725 487 429 169 150 39
7
BsrDI GCAATG_nn'
Cuts at: 65 1248 5162 5336 65
Size: 1183 3914 174 1396
Fragments arranged by size:
3914 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 2160 3239 3293 5311 5498 1700
Size: 110 299 51 1079 54 2018 187 2869
Fragments arranged by size:
2869 2018 1079 299 187 110 54 51
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6667
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6667
BssSI C'ACGA_G
Cuts at: 2816 4727 5739 2816
Size: 1911 1012 3744
Fragments arranged by size:
3744 1911 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3256 3820 794
Size: 2462 564 3641
Fragments arranged by size:
3641 2462 564
BstBI TT'CG_AA
Cuts at: 5471 5471
Size: 6667
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6667
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 2840 3307 2284
Size: 556 467 5644
Fragments arranged by size:
5644 556 467
BstYI r'GATC_y
Cuts at: 1972 3997 4364 4749 4766 6218 6304 6316 6662
Size: 2025 367 385 17 1452 86 12 346
Cuts at: 6662 1972
Size: 1977
Fragments arranged by size:
2025 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3283 3283
Size: 6667
BtgI C'CryG_G
Cuts at: 513 1065 3072 3163 3228 3268 3474 513
Size: 552 2007 91 65 40 206 3706
Fragments arranged by size:
3706 2007 552 206 91 65 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5928
Fragments arranged by size:
5928 683 56
BtsI GCAGTG_nn'
Cuts at: 2785 4183 4977 4997 2785
Size: 1398 794 20 4455
Fragments arranged by size:
4455 1398 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6667
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6632
DraI TTT'AAA
Cuts at: 3971 3971
Size: 6667
DraIII CAC_nnn'GTG
Cuts at: 3229 3229
Size: 6667
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5674 809
Size: 507 4358 1802
Fragments arranged by size:
4358 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 3265 3480
Size: 54 1425 50 275 18 294 1141 215
Cuts at: 3480 3660 4114 5003 6632 8
Size: 180 454 889 1629 43
Fragments arranged by size:
1629 1425 1141 889 454 294 275 215
180 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3480 4114 1537
Size: 275 1668 634 4090
Fragments arranged by size:
4090 1668 634 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2275 3864 4597 1353
Size: 922 1589 733 3423
Fragments arranged by size:
3423 1589 922 733
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2494 2599 5254 5628 5774 387
Size: 1064 138 905 105 2655 374 146 1280
Fragments arranged by size:
2655 1280 1064 905 374 146 138 105
EcoICRI GAG'CTC
Cuts at: 2050 2683 2050
Size: 633 6034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2212 2707 2902 3587 4730 5327
Size: 393 421 159 495 195 685 1143 597
Cuts at: 5327 6114 6382 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1143 787 685 597 495 421 393
268 195 159
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 3117 3197 3782 2366
Size: 751 80 585 5251
Fragments arranged by size:
5251 751 585 80
EcoO109I rG'GnC_Cy
Cuts at: 2412 2439 2530 3088 3571 2412
Size: 27 91 558 483 5508
Fragments arranged by size:
5508 558 483 91 27
EcoRI G'AATT_C
Cuts at: 3976 4354 3976
Size: 378 6289
FseI GG_CCGG'CC
Cuts at: 2113 2113
Size: 6667
FspI TGC'GCA
Cuts at: 5173 5173
Size: 6667
HaeII r_GCGC'y
Cuts at: 1678 2030 2325 3278 3655 5814 1678
Size: 352 295 953 377 2159 2531
Fragments arranged by size:
2531 2159 953 377 352 295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2280 2312 3512 3544 5308
Size: 32 135 32 708 32 1200 32 1764
Cuts at: 5308 5340 5382 5414 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1764 1200 708 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4011 4257 669
Size: 988 281 2073 246 3079
Fragments arranged by size:
3079 2073 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6667
HpaI GTT'AAC
Cuts at: 4257 4257
Size: 6667
KasI G'GCGC_C
Cuts at: 2026 2321 2026
Size: 295 6372
KpnI G_GTAC'C
Cuts at: 3992 3992
Size: 6667
MfeI C'AATT_G
Cuts at: 4266 4266
Size: 6667
MluI A'CGCG_T
Cuts at: 6543 6543
Size: 6667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3313 3874 5781 5965 1305
Size: 2008 561 1907 184 2007
Fragments arranged by size:
2008 2007 1907 561 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3267 3662 10
Size: 54 1425 343 1435 395 3015
Fragments arranged by size:
3015 1435 1425 395 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3209 4625 4984 5143 518
Size: 648 2043 1416 359 159 2042
Fragments arranged by size:
2043 2042 1416 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2187 2743 2836 2940 2978 3533 4083
Size: 480 101 556 93 104 38 555 550
Cuts at: 4083 4760 5908 6153 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 556 555 550 480 245
104 101 93 38
NaeI GCC'GGC
Cuts at: 2111 3241 2111
Size: 1130 5537
NarI GG'CG_CC
Cuts at: 2027 2322 2027
Size: 295 6372
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6115
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6667
NgoMIV G'CCGG_C
Cuts at: 2109 3239 2109
Size: 1130 5537
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6667
NotI GC'GGCC_GC
Cuts at: 3480 4114 3480
Size: 634 6033
NspI r_CATG'y
Cuts at: 1132 1229 3250 3730 4025 5570 1132
Size: 97 2021 480 295 1545 2229
Fragments arranged by size:
2229 2021 1545 480 295 97
PasI CC'CwG_GG
Cuts at: 1348 3085 1348
Size: 1737 4930
PciI A'CATG_T
Cuts at: 5566 5566
Size: 6667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3091 3229 1108
Size: 1983 138 4546
Fragments arranged by size:
4546 1983 138
PfoI T'CCnGG_A
Cuts at: 2061 2061
Size: 6667
PmeI GTTT'AAAC
Cuts at: 3971 3971
Size: 6667
PmlI CAC'GTG
Cuts at: 3211 3211
Size: 6667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4736 4768 6249 6281 4736
Size: 32 1481 32 5122
Fragments arranged by size:
5122 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2439 2530 2439
Size: 91 6576
PshAI GACnn'nnGTC
Cuts at: 1613 3156 1613
Size: 1543 5124
PsiI TTA'TAA
Cuts at: 4237 4237
Size: 6667
PspOMI G'GGCC_C
Cuts at: 2411 2714 3579 2411
Size: 303 865 5499
Fragments arranged by size:
5499 865 303
PspXI vC'TCGA_Gb
Cuts at: 3114 3114
Size: 6667
PstI C_TGCA'G
Cuts at: 830 3174 4019 830
Size: 2344 845 3478
Fragments arranged by size:
3478 2344 845
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6667
PvuII CAG'CTG
Cuts at: 2836 2978 2836
Size: 142 6525
SacI G_AGCT'C
Cuts at: 2052 2685 2052
Size: 633 6034
SalI G'TCGA_C
Cuts at: 1936 4009 1936
Size: 2073 4594
SbfI CC_TGCA'GG
Cuts at: 4019 4019
Size: 6667
ScaI AGT'ACT
Cuts at: 2450 4915 6627 2450
Size: 2465 1712 2490
Fragments arranged by size:
2490 2465 1712
SfcI C'TryA_G
Cuts at: 826 1045 3170 3448 4015 5150 5831 6022 826
Size: 219 2125 278 567 1135 681 191 1471
Fragments arranged by size:
2125 1471 1135 681 567 278 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3578 3578
Size: 6667
SfoI GGC'GCC
Cuts at: 2028 2323 2028
Size: 295 6372
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6667
SmaI CCC'GGG
Cuts at: 1872 2410 2697 3578 3674 3994 1872
Size: 538 287 881 96 320 4545
Fragments arranged by size:
4545 881 538 320 287 96
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 3037 3114 4771 5672 5934
Size: 197 926 210 884 77 1657 901 262
Cuts at: 5934 820
Size: 1553
Fragments arranged by size:
1657 1553 926 901 884 262 210 197
77
SnaBI TAC'GTA
Cuts at: 493 4458 493
Size: 3965 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6667
SphI G_CATG'C
Cuts at: 1132 4025 1132
Size: 2893 3774
SspI AAT'ATT
Cuts at: 5 52 4591 5
Size: 47 4539 2081
Fragments arranged by size:
4539 2081 47
StuI AGG'CCT
Cuts at: 1517 3327 3707 3933 1517
Size: 1810 380 226 4251
Fragments arranged by size:
4251 1810 380 226
StyI C'CwwG_G
Cuts at: 513 1065 4106 513
Size: 552 3041 3074
Fragments arranged by size:
3074 3041 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 2578 2714 3299 3784 3942 4694
Size: 1255 675 323 136 585 485 158 752
Cuts at: 4694 4711 4864 5049 5494 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 752 675 585 485 445 323
185 158 153 136 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 4913 6625
Size: 274 80 33 51 1443 471 2465 1712
Cuts at: 6625 96
Size: 138
Fragments arranged by size:
2465 1712 1443 471 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2835 2867 6627 6659 2835
Size: 32 3760 32 2843
Fragments arranged by size:
3760 2843 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6667
XbaI T'CTAG_A
Cuts at: 4003 4003
Size: 6667
XhoI C'TCGA_G
Cuts at: 1943 3114 1943
Size: 1171 5496
XmaI C'CCGG_G
Cuts at: 1870 2408 2695 3576 3672 3992 1870
Size: 538 287 881 96 320 4545
Fragments arranged by size:
4545 881 538 320 287 96
XmnI GAAnn'nnTTC
Cuts at: 4796 4796
Size: 6667
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4475 276
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BstZ17I
EcoRV FalI FspAI NruI NsiI PacI PsrI RsrII
SacII SanDI SapI SexAI SrfI SwaI XcmI