Restriction map for
pFN21AB5147
(Circular) (Six-base) MAPSORT of: pFN21AB5147.seq Check: 8707 from: 1 to: 5767
pFN21AB5147 5767 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3110 1937
Size: 1173 4594
Acc65I G'GTAC_C
Cuts at: 2378 2527 3088 2378
Size: 149 561 5057
Fragments arranged by size:
5057 561 149
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 3830 5214 5482 1632
Size: 449 1749 1384 268 1917
Fragments arranged by size:
1917 1749 1384 449 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 3055 4666 5643 1203
Size: 1852 1611 977 1327
Fragments arranged by size:
1852 1611 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 2299 3510 5082 2299
Size: 1211 1572 2984
Fragments arranged by size:
2984 1572 1211
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 1870
Size: 73 1149 4545
Fragments arranged by size:
4545 1149 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2378 2527 3088 4543 618
Size: 325 217 627 591 149 561 1455 1842
Fragments arranged by size:
1842 1455 627 591 561 325 217 149
BanII G_rGCy'C
Cuts at: 1391 2098 3086 1391
Size: 707 988 4072
Fragments arranged by size:
4072 988 707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2936 928
Size: 971 1037 3759
Fragments arranged by size:
3759 1037 971
BbvCI CC'TCA_GC
Cuts at: 2304 2844 2304
Size: 540 5227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2693 3659 4869 2693
Size: 966 1210 3591
Fragments arranged by size:
3591 1210 966
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BfrBI ATG'CAT
Cuts at: 2765 2765
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4378 136
Size: 107 122 71 631 605 2706 1525
Fragments arranged by size:
2706 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 3828 4984 1790
Size: 2038 1156 2573
Fragments arranged by size:
2573 2038 1156
BmgBI CAC'GTC
Cuts at: 1318 2605 1318
Size: 1287 4480
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2360 3018 3498
Size: 416 53 83 399 24 943 658 480
Cuts at: 3498 4456 442
Size: 958 1753
Fragments arranged by size:
1753 958 943 658 480 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2241 2241
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2021 2164 2211 2769 4427 1239
Size: 782 143 47 558 1658 2579
Fragments arranged by size:
2579 1658 782 558 143 47
Bpu10I CC'TnA_GC
Cuts at: 2304 2844 2304
Size: 540 5227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2352 4430 882
Size: 617 853 2078 2219
Fragments arranged by size:
2219 2078 853 617
BsaAI yAC'GTr
Cuts at: 493 1204 3056 3558 493
Size: 711 1852 502 2702
Fragments arranged by size:
2702 1852 711 502
BsaBI GATnn'nnATC
Cuts at: 2670 5761 2670
Size: 3091 2676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2520 2550 2767 2782 2797
Size: 30 165 30 1117 30 217 15 15
Cuts at: 2797 2812 1178
Size: 15 4133
Fragments arranged by size:
4133 1117 217 165 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2370 2495 1186
Size: 838 346 125 4458
Fragments arranged by size:
4458 838 346 125
BseYI C'CCAG_C
Cuts at: 4970 5466 4970
Size: 496 5271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5194
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3828 3913 4984 3828
Size: 85 1071 4611
Fragments arranged by size:
4611 1071 85
BsmI GAATG_Cn'
Cuts at: 2050 3278 3371 2050
Size: 1228 93 4446
Fragments arranged by size:
4446 1228 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2036 2595 2036
Size: 559 5208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 3086 3828 3913 4984 1391
Size: 399 308 988 742 85 1071 2174
Fragments arranged by size:
2174 1071 988 742 399 308 85
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 844
Size: 2278 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4262 4436 65
Size: 1183 3014 174 1396
Fragments arranged by size:
3014 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2881 3097 3464 3849 3866 5318 5404 5416
Size: 909 216 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 909 385 367 346 216 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2012 2012
Size: 5767
BtgI C'CryG_G
Cuts at: 513 1065 2079 2482 513
Size: 552 1014 403 3798
Fragments arranged by size:
3798 1014 552 403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2401 3283 4077 4097 2401
Size: 882 794 20 4071
Fragments arranged by size:
4071 882 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 2631 3071 2631
Size: 440 5327
DraIII CAC_nnn'GTG
Cuts at: 2080 2080
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2389 2907 3214
Size: 54 1425 50 275 18 559 518 307
Cuts at: 3214 4103 5732 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 559 518 307 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2575 2916 3697 1353
Size: 1222 341 781 3423
Fragments arranged by size:
3423 1222 781 341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 2081 2164 2211 2769 3830 4427
Size: 393 389 60 83 47 558 1061 597
Cuts at: 4427 5214 5482 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1061 787 597 558 393 389 268
83 60 47
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
EcoRV GAT'ATC
Cuts at: 2644 2644
Size: 5767
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 4914 1678
Size: 3236 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2251 2283 2667 2699 4408
Size: 32 135 32 679 32 384 32 1709
Cuts at: 4408 4440 4482 4514 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1709 679 384 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2968 748
Size: 2220 3547
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 2382 2531 3092 2382
Size: 149 561 5057
Fragments arranged by size:
5057 561 149
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2391 2909 10
Size: 54 1425 343 559 518 2868
Fragments arranged by size:
2868 1425 559 518 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3725 4084 4243 518
Size: 648 2559 359 159 2042
Fragments arranged by size:
2559 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2144 2340 3183 3860 5008 5253 1606
Size: 538 196 843 677 1148 245 2120
Fragments arranged by size:
2120 1148 843 677 538 245 196
NcoI C'CATG_G
Cuts at: 513 1065 2482 513
Size: 552 1417 3798
Fragments arranged by size:
3798 1417 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NsiI A_TGCA'T
Cuts at: 2767 2767
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 2836 3125 4670 1132
Size: 97 1607 289 1545 2229
Fragments arranged by size:
2229 1607 1545 289 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 2299 2368 1108
Size: 972 219 69 4507
Fragments arranged by size:
4507 972 219 69
PfoI T'CCnGG_A
Cuts at: 2549 2549
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PmlI CAC'GTG
Cuts at: 3056 3056
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PstI C_TGCA'G
Cuts at: 830 3119 830
Size: 2289 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2340 2340
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2335 2516 2934 3115 4250 4931 5122
Size: 219 1290 181 418 181 1135 681 191
Cuts at: 5122 826
Size: 1471
Fragments arranged by size:
1471 1290 1135 681 418 219 191 181
181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2482 3206 513
Size: 552 1417 724 3074
Fragments arranged by size:
3074 1417 724 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3794 3811 3964 4149 4594 325
Size: 1255 2214 17 153 185 445 1498
Fragments arranged by size:
2214 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4013 5725 96
Size: 274 80 33 51 1443 2036 1712 138
Fragments arranged by size:
2036 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2782 2814 5727 5759 2782
Size: 32 2913 32 2790
Fragments arranged by size:
2913 2790 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2296 2547 2296
Size: 251 5516
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BstXI BstZ17I EcoICRI EcoNI EcoO109I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI