Restriction map for
pFN21AB5141
(Circular) (Six-base) MAPSORT of: pFN21AB5141.seq Check: 4799 from: 1 to: 6274
pFN21AB5141 6274 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2161 2326 2161
Size: 165 6109
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4084 278
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3617 1937
Size: 1680 4594
Acc65I G'GTAC_C
Cuts at: 3595 3595
Size: 6274
AclI AA'CG_TT
Cuts at: 1563 4401 4774 1563
Size: 2838 373 3063
Fragments arranged by size:
3063 2838 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 3466 4337 5721 5989 1632
Size: 977 857 871 1384 268 1917
Fragments arranged by size:
1917 1384 977 871 857 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6077
AflIII A'CryG_T
Cuts at: 1203 2919 3393 5173 6150 1203
Size: 1716 474 1780 977 1327
Fragments arranged by size:
1780 1716 1327 977 474
AgeI A'CCGG_T
Cuts at: 5105 5105
Size: 6274
AhdI GACnn_n'nnGTC
Cuts at: 5003 5003
Size: 6274
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3323 1184
Size: 2139 4135
AlwNI CAG_nnn'CTG
Cuts at: 2061 3143 4017 5589 2061
Size: 1082 874 1572 2746
Fragments arranged by size:
2746 1572 1082 874
ApaI G_GGCC'C
Cuts at: 2979 2979
Size: 6274
ApaLI G'TGCA_C
Cuts at: 4331 5487 4331
Size: 1156 5118
ApoI r'AATT_y
Cuts at: 1493 3291 3583 3809 3961 6070 1493
Size: 1798 292 226 152 2109 1697
Fragments arranged by size:
2109 1798 1697 292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4828 160
Size: 872 3796 1606
Fragments arranged by size:
3796 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2234 2978 3599 1870
Size: 73 177 114 744 621 4545
Fragments arranged by size:
4545 744 621 177 114 73
BamHI G'GATC_C
Cuts at: 2415 3604 3971 2415
Size: 1189 367 4718
Fragments arranged by size:
4718 1189 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3595 5050 618
Size: 325 217 627 1808 1455 1842
Fragments arranged by size:
1842 1808 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2235 2682 2748 2979 3003 3593 1391
Size: 844 447 66 231 24 590 4072
Fragments arranged by size:
4072 844 590 447 231 66 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3273 928
Size: 971 1374 3929
Fragments arranged by size:
3929 1374 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3418 3452 4465 4499 1903
Size: 34 50 34 1397 34 1013 34 3678
Fragments arranged by size:
3678 1397 1013 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4166 5376 4166
Size: 1210 5064
BclI T'GATC_A
Cuts at: 1548 3072 1548
Size: 1524 4750
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 4885 136
Size: 107 122 71 631 605 421 2792 1525
Fragments arranged by size:
2792 1525 631 605 421 122 107 71
BglII A'GATC_T
Cuts at: 6269 6269
Size: 6274
BlpI GC'TnA_GC
Cuts at: 3691 3691
Size: 6274
Bme1580I G_kGCm'C
Cuts at: 1790 2410 2979 3134 3444 4335 5491 1790
Size: 620 569 155 310 891 1156 2573
Fragments arranged by size:
2573 1156 891 620 569 310 155
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6274
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3131 4005 4963
Size: 416 53 83 399 24 1714 874 958
Cuts at: 4963 442
Size: 1753
Fragments arranged by size:
1753 1714 958 874 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2463 1056
Size: 1407 4867
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 6274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2913 2971 3372 3437 3486 3581 4934
Size: 935 739 58 401 65 49 95 1353
Cuts at: 4934 1239
Size: 2579
Fragments arranged by size:
2579 1353 935 739 401 95 65 58
49
Bpu10I CC'TnA_GC
Cuts at: 3369 3369
Size: 6274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 2198 4399 5264 5562 2093
Size: 105 2201 865 298 2805
Fragments arranged by size:
2805 2201 865 298 105
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4937 882
Size: 617 3438 2219
Fragments arranged by size:
3438 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4065 493
Size: 711 2861 2702
Fragments arranged by size:
2861 2702 711
BsaBI GATnn'nnATC
Cuts at: 2639 3491 6268 2639
Size: 852 2777 2645
Fragments arranged by size:
2777 2645 852
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2295 2685 4081 4463
Size: 53 83 186 936 762 390 1396 382
Cuts at: 4463 5098 275
Size: 635 1451
Fragments arranged by size:
1451 1396 936 762 635 390 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4707 5105 5379 5526 5937 4707
Size: 398 274 147 411 5044
Fragments arranged by size:
5044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3005 3035 1178
Size: 30 165 30 1602 30 4417
Fragments arranged by size:
4417 1602 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2729 3069 3206 3434 1186
Size: 1543 340 137 228 4026
Fragments arranged by size:
4026 1543 340 228 137
BseYI C'CCAG_C
Cuts at: 2054 2378 2724 5477 5973 2054
Size: 324 346 2753 496 2355
Fragments arranged by size:
2753 2355 496 346 324
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2780 3065 1163
Size: 573 1044 285 4372
Fragments arranged by size:
4372 1044 573 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 3724 4485 5513 1540
Size: 275 186 87 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186 87
BsiHKAI G_wGCw'C
Cuts at: 2109 2214 2235 2682 2748 2796 3003 4335 4420
Size: 105 21 447 66 48 207 1332 85
Cuts at: 4420 5491 2109
Size: 1071 2892
Fragments arranged by size:
2892 1332 1071 447 207 105 85 66
48 21
BsmI GAATG_Cn'
Cuts at: 3785 3878 3785
Size: 93 6181
BsmBI CGTCTCn'nnnn_
Cuts at: 2605 2605
Size: 6274
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2214 2235 2410 2682 2748 2796
Size: 399 319 105 21 175 272 66 48
Cuts at: 2796 2979 3003 3134 3444 3593 4335 4420 5491
Size: 183 24 131 310 149 742 85 1071
Cuts at: 5491 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 319 310 272 183
175 149 131 105 85 66 48 24
21
BspEI T'CCGG_A
Cuts at: 5937 5937
Size: 6274
BspHI T'CATG_A
Cuts at: 4163 5893 4163
Size: 1730 4544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2161 2326 2373 3087 3629 844
Size: 1317 165 47 714 542 3489
Fragments arranged by size:
3489 1317 714 542 165 47
BsrBI CCG'CTC
Cuts at: 1394 1823 4161 1394
Size: 429 2338 3507
Fragments arranged by size:
3507 2338 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4769 4943 65
Size: 1183 3521 174 1396
Fragments arranged by size:
3521 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 2219 4918 5105 1700
Size: 110 320 89 2699 187 2869
Fragments arranged by size:
2869 2699 320 187 110 89
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6274
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6274
BssSI C'ACGA_G
Cuts at: 4334 5346 4334
Size: 1012 5262
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2844 794
Size: 2050 4224
BstBI TT'CG_AA
Cuts at: 5078 5078
Size: 6274
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6274
BstXI CCAn_nnnn'nTGG
Cuts at: 2995 3358 2995
Size: 363 5911
BstYI r'GATC_y
Cuts at: 1972 2415 2592 3336 3604 3971 4356 4373 5825
Size: 443 177 744 268 367 385 17 1452
Cuts at: 5825 5911 5923 6269 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 744 443 385 367 346 268
177 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2787 2787
Size: 6274
BtgI C'CryG_G
Cuts at: 513 1065 2556 3156 513
Size: 552 1491 600 3631
Fragments arranged by size:
3631 1491 600 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5535
Fragments arranged by size:
5535 683 56
BtsI GCAGTG_nn'
Cuts at: 3295 3454 3790 4584 4604 3295
Size: 159 336 794 20 4965
Fragments arranged by size:
4965 794 336 159 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6239
DraI TTT'AAA
Cuts at: 3578 3578
Size: 6274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5281 809
Size: 507 3965 1802
Fragments arranged by size:
3965 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 3243 3721
Size: 54 1425 50 275 18 255 1158 478
Cuts at: 3721 4610 6239 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1158 889 478 275 255 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2085 3721 1537
Size: 275 273 1636 4090
Fragments arranged by size:
4090 1636 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 2871 4204 1353
Size: 1518 1333 3423
Fragments arranged by size:
3423 1518 1333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2428 4861 5235 5381 387
Size: 1064 138 839 2433 374 146 1280
Fragments arranged by size:
2433 1280 1064 839 374 146 138
EcoICRI GAG'CTC
Cuts at: 2233 2680 2746 3001 2233
Size: 447 66 255 5506
Fragments arranged by size:
5506 447 255 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2609 2913 2971 3372 3437 3466
Size: 393 542 435 304 58 401 65 29
Cuts at: 3466 3486 3581 4337 4934 5721 5989 1239
Size: 20 95 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 597 542 435 401 393
304 268 95 65 58 29 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2711 2333
Size: 378 5896
EcoO109I rG'GnC_Cy
Cuts at: 2201 2448 2975 2201
Size: 247 527 5500
Fragments arranged by size:
5500 527 247
EcoRI G'AATT_C
Cuts at: 3291 3583 3961 3291
Size: 292 378 5604
Fragments arranged by size:
5604 378 292
FspI TGC'GCA
Cuts at: 4780 4780
Size: 6274
HaeII r_GCGC'y
Cuts at: 1678 2024 2732 5421 1678
Size: 346 708 2689 2531
Fragments arranged by size:
2689 2531 708 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 3209 3241 4915
Size: 32 135 32 699 32 906 32 1674
Cuts at: 4915 4947 4989 5021 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1674 906 699 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 3618 3864 669
Size: 988 281 535 1145 246 3079
Fragments arranged by size:
3079 1145 988 535 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6274
HpaI GTT'AAC
Cuts at: 3864 3864
Size: 6274
KpnI G_GTAC'C
Cuts at: 3599 3599
Size: 6274
MfeI C'AATT_G
Cuts at: 3873 3873
Size: 6274
MluI A'CGCG_T
Cuts at: 6150 6150
Size: 6274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5388 5572 1305
Size: 4083 184 2007
Fragments arranged by size:
4083 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3245 10
Size: 54 1425 343 1413 3039
Fragments arranged by size:
3039 1425 1413 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2693 3068 3167 4232 4591 4750 518
Size: 648 1527 375 99 1065 359 159 2042
Fragments arranged by size:
2042 1527 1065 648 375 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 2011 2245 2666 3103 3690 4367 5515 5760
Size: 405 234 421 437 587 677 1148 245
Cuts at: 5760 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 587 437 421 405 245
234
NaeI GCC'GGC
Cuts at: 2132 2221 2132
Size: 89 6185
NcoI C'CATG_G
Cuts at: 513 1065 3156 513
Size: 552 2091 3631
Fragments arranged by size:
3631 2091 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6274
NgoMIV G'CCGG_C
Cuts at: 2130 2219 2130
Size: 89 6185
NheI G'CTAG_C
Cuts at: 1052 2459 1052
Size: 1407 4867
NotI GC'GGCC_GC
Cuts at: 2085 3721 2085
Size: 1636 4638
NspI r_CATG'y
Cuts at: 1132 1229 3397 3632 5177 1132
Size: 97 2168 235 1545 2229
Fragments arranged by size:
2229 2168 1545 235 97
PasI CC'CwG_GG
Cuts at: 1348 2810 1348
Size: 1462 4812
PciI A'CATG_T
Cuts at: 3393 5173 3393
Size: 1780 4494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2061 2305 3143 3382 1108
Size: 953 244 838 239 4000
Fragments arranged by size:
4000 953 838 244 239
PfoI T'CCnGG_A
Cuts at: 3414 3558 3414
Size: 144 6130
PmeI GTTT'AAAC
Cuts at: 3578 3578
Size: 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 5856 5888 4343
Size: 32 1481 32 4729
Fragments arranged by size:
4729 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2201 2448 2201
Size: 247 6027
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6274
PsiI TTA'TAA
Cuts at: 3844 3844
Size: 6274
PspOMI G'GGCC_C
Cuts at: 2975 2975
Size: 6274
PspXI vC'TCGA_Gb
Cuts at: 2234 2234
Size: 6274
PstI C_TGCA'G
Cuts at: 830 2244 2424 3531 3626 830
Size: 1414 180 1107 95 3478
Fragments arranged by size:
3478 1414 1107 180 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6274
PvuII CAG'CTG
Cuts at: 2666 3103 2666
Size: 437 5837
SacI G_AGCT'C
Cuts at: 2235 2682 2748 3003 2235
Size: 447 66 255 5506
Fragments arranged by size:
5506 447 255 66
SalI G'TCGA_C
Cuts at: 1936 3616 1936
Size: 1680 4594
SanDI GG'GwC_CC
Cuts at: 2201 2201
Size: 6274
SbfI CC_TGCA'GG
Cuts at: 2424 3626 2424
Size: 1202 5072
ScaI AGT'ACT
Cuts at: 2984 4522 6234 2984
Size: 1538 1712 3024
Fragments arranged by size:
3024 1712 1538
SfcI C'TryA_G
Cuts at: 826 1045 2240 2420 2452 3527 3622 4757 5438
Size: 219 1195 180 32 1075 95 1135 681
Cuts at: 5438 5629 826
Size: 191 1471
Fragments arranged by size:
1471 1195 1135 1075 681 219 191 180
95 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 2093 2093
Size: 6274
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6274
SmaI CCC'GGG
Cuts at: 1872 3601 1872
Size: 1729 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 2213 2234 4378 5279 5541
Size: 197 926 165 105 21 2144 901 262
Cuts at: 5541 820
Size: 1553
Fragments arranged by size:
2144 1553 926 901 262 197 165 105
21
SnaBI TAC'GTA
Cuts at: 493 4065 493
Size: 3572 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6274
SphI G_CATG'C
Cuts at: 1132 3632 1132
Size: 2500 3774
SspI AAT'ATT
Cuts at: 5 52 4198 5
Size: 47 4146 2081
Fragments arranged by size:
4146 2081 47
StuI AGG'CCT
Cuts at: 1517 2096 1517
Size: 579 5695
StyI C'CwwG_G
Cuts at: 513 1065 3024 3156 3713 513
Size: 552 1959 132 557 3074
Fragments arranged by size:
3074 1959 557 552 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3217 4301 4318 4471 4656 5101 325
Size: 1255 1637 1084 17 153 185 445 1498
Fragments arranged by size:
1637 1498 1255 1084 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2922 2982 4520
Size: 274 80 33 51 1443 945 60 1538
Cuts at: 4520 6232 96
Size: 1712 138
Fragments arranged by size:
1712 1538 1443 945 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2193 2225 3005 3037 6234 6266 2193
Size: 32 780 32 3197 32 2201
Fragments arranged by size:
3197 2201 780 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3164 1316
Size: 1848 4426
XbaI T'CTAG_A
Cuts at: 3610 3610
Size: 6274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3031 3031
Size: 6274
XhoI C'TCGA_G
Cuts at: 1943 2234 1943
Size: 291 5983
XmaI C'CCGG_G
Cuts at: 1870 3599 1870
Size: 1729 4545
XmnI GAAnn'nnTTC
Cuts at: 3542 4403 3542
Size: 861 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4082 276
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PsrI RsrII SacII
SapI SexAI SfoI SrfI SwaI